RESUMEN

Flavonoids are compounds with a benzopyranic structure that exhibits multiple pharmacological activities. They are known for their venotonic activity, but their mechanism of action remains unclear. It is thought that, as this mechanism is mediated by prostaglandins, these compounds may interfere with the arachidonic acid (AA) cascade. These assays are designed to measure the antiplatelet aggregation capacity of quercetin, rutin, diosmetin, diosmin, and hidrosmin, as well as to evaluate a potential structure-activity ratio. In this paper, several studies on platelet aggregation at different concentrations (from 0.33 mM to 1.5 mM) of different flavone compounds are conducted, measuring platelet aggregation by impedance aggregometry, and the cyclooxygenase (COX) activity by metabolites generated, including the activity of the pure recombinant enzyme in the presence of these polyphenols. The results obtained showed that quercetin and diosmetin aglycones have a greater antiplatelet effect and inhibit the COX enzyme activity to a greater extent than their heterosides; however, the fact that greater inhibition of the pure recombinant enzyme was achieved by heterosides suggests that these compounds may have difficulty in crossing biological membranes. In any case, in view of the results obtained, it can be concluded that flavonoids could be useful as coadjuvants in the treatment of cardiovascular pathologies.

Asunto(s)

RESUMEN

BACKGROUND: Progressive ischemic stroke is a common cerebrovascular disease with high morbidity. This study aimed to investigate the relationship between changes of Thromboxane B2 (TXB2), 6-keto-prostaglandin Fla (6-k-PGFla), and blood glucose (BG) levels with progressive ischemic stroke. METHODS: A total of 106 patients with progressive ischemic stroke admitted to our hospital from December 2016 to December 2018 were recruited as the observation group, and 110 patients who received physical examination in our hospital during the same period were selected as the control group. The levels of TXB2, 6-k-PGFla, and BG in different groups were compared, the related risk factors affecting the prognosis of patients with progressive ischemic stroke were analyzed, and the receiver operating characteristic (ROC) curve was drawn to evaluate the predictive value of TXB2, 6-k-PGFla, and BG for the prognostic mortality of patients with progressive ischemic stroke. RESULTS: The levels of TXB2, 6-k-PGFla, and BG in the observation group were significantly higher than those in the control group (P<0.05). The prognostic mortality of participants with abnormally increased expression of TXB2, 6-k-PGFla, and BG was significantly higher than that of patients with normal expression of TXB2, 6-k-PGFla, and BG (P<0.05). Hypertension, diabetes, collateral circulatory disorders, hyperlipidemia, TXB2 (abnormal increase), 6-k-PGFla (abnormal increase), and BG (abnormal increase) were risk factors affecting the prognosis of patients with progressive ischemic stroke (P<0.05). The area under the curve (AUC) of the ROC curve showed that TXB2, 6-k-PGFla, BG, and the combination of them were 0.846, 0.893, 0.835, and 0.971, respectively, showing that the AUC of the combination of them was the largest. CONCLUSIONS: Hypertension, diabetes, collateral circulatory disorders, hyperlipidemia, TXB2 (abnormal increase), 6-k-PGFla (abnormal increase), and BG (abnormal increase) are risk factors affecting the prognosis of patients with progressive ischemic stroke. The combined detection of the 3 indicators showed high sensitivity and specificity in evaluating the prognostic mortality of patients with progressive ischemic stroke, indicating that clinicians might improve the early diagnosis rate of progressive ischemic stroke by combining the detection of TXB2, 6-k-PGFla, and BG to predict the prognosis of patients.

Asunto(s)

RESUMEN

@#Chemical constituents and biological activities of the Mitrella kentii leaf oil were investigated in this study. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were used to determine the chemical constituents of the oil. The oil was evaluated for its ability to inhibit prostaglandin E2 (PGE2 ) and thromboxane B2 (TXB2 ) productions in human whole blood using a radioimmunoassay technique. Its inhibitory effect on plateletactivating factor (PAF) receptor binding with rabbit platelets using 3 H-PAF as a ligand and its free radical scavenging effect on DPPH were also investigated. Caryophyllene oxide (33.8%w/w), E,Z-farnesol (6.9%), benzyl benzoate (6.5%w/w) and viridiflorol (6.5%w/w) were among the major components of the oil. Even though weak inhibitory activities were observed in both PGE2 and TXB2 assays, significant results were obtained in both PAF receptor binding inhibition and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging effect with IC50 value of 6.6 µg/mL and 155.6 µg/mL respectively. These promising activities warrant the development of the oil as an anti-inflammatory agent.

RESUMEN

Little is known about the pathomechanism of pulmonary infections caused by Acanthamoeba sp. Therefore, the aim of this study was to determine whether Acanthamoeba sp. may affect the expression and activity of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), resulting in the altered levels of their main products, prostaglandins (PGE2) and thromboxane B2 (TXB2), in lungs of immunocompetent or immunosuppressed hosts. Acanthamoeba sp. induced a strong expression of COX-1 and COX-2 proteins in the lungs of immunocompetent mice, which, however, did not result in significant differences in the expression of PGE2 and TXB2. Our immunohistochemical analysis showed that immunosuppression induced by glucocorticoids in Acanthamoeba sp.-infected mice caused a decrease in COX-1 and COX-2 (not at the beginning of infection) in lung tissue. These results suggest that similar to COX-2, COX-1 is an important mediator of the pathophysiology in experimental pulmonary acanthamoebiasis. We suggest that the signaling pathways important for Acanthamoeba sp. induction of lung infection might interact with each other and depend on the host immune status.

Asunto(s)

RESUMEN

Recent studies have shown promising results concerning the effectiveness of 3D plates in terms of stabilization of condylar fractures. Despite the use of new techniques and new materials, we can still observe certain side effects, including the immune reaction of the body, which may lead to the excessive inflammation. The aim of this paper was to determine how the production of prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) in THP-1 monocytes/macrophages is influenced by the titanium 3D plates and dedicated screws. The experiments were conducted on THP-1 monocytic cell line and macrophages derived from a THP-1cells. The concentrations of PGE2 and TXB2 released were measured by using immunoassay kit. Verification of plate-induced activation of THP-1 monocytes and macrophages and initiation of inflammatory reaction was conducted by flow cytometry. Despite some differences in the content of the implant devices our results showed that these plates did not statistically significantly increase the production of these prostanoids. Osteosynthesis of condylar fractures using 3D titanium mini-plates seems to be a good alternative to traditional plates due to their lack of stimulating the cyclooxygenase-dependent production of prostanoids; limiting the development of inflammatory reactions.

Asunto(s)

RESUMEN

The aim of this study was to determine whether Hymenolepis diminuta may affect the expression and activity of cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2), resulting in the altered levels of their main products - prostaglandins (PGE2) and thromboxane B2 (TXB2). The study used the same experimental model as in our previous studies in which we had observed changes in the transepithelial ion transport, tight junctions and in the indicators of oxidative stress, in both small and large intestines of rats infected with H. diminuta. In this paper, we investigated not only the site of immediate presence of the tapeworm (jejunum), but also a distant site (colon). Inflammation related to H. diminuta infection is associated with the increased expression and activation of cyclooxygenase (COX), enzyme responsible for the synthesis of PGE2 and TXB2, local hormones contributing to the enhanced inflammatory reaction in the jejunum and colon in the infected rats. The increased COX expression and activity is probably caused by the increased levels of free radicals and the weakening of the host's antioxidant defense induced by the presence of the parasite. Our immunohistochemical analysis showed that H. diminuta infection affected not only the intensity of the immunodetection of COX but also the enzyme protein localization within intestinal epithelial cells - from the entire cytoplasm to apical/basal regions of cells, or even to the nucleus.

Asunto(s)

RESUMEN

@#ObjectiveTo study the relevant pathogenic factors, Thromboxane B2 (TXB2), oxidized low density lipoprotein (OxLDL), lipoprotein(a) (Lp(a)), and homocysteine (Hcy), in patients with cerebral infarction and the correlation among them. Methods205 patients and 40 health persons (the control) were measured with the plasma TXB2, 6-keto-prostaglandin F1α (PGF1α) and TXB2/6-keto-PGF1α (T/6-K), OxLDL, Lp(a), Hcy within 24 h. Results and ConclusionThe levels of plasma TXB2, T/6-K，OxLDL, Lp(a), and Hcy significantly increased compared with the controls (P<0.01). OxLDL was correlated with Lp(a); TXB2 was correlated with T/6-K and Hcy; T/6-K was correlated with OxLDL, Lp（a）.

RESUMEN

@#Objective To assess the level of plasma thromboxane B2(TXB2),6-keto-prostaglandin F1α(6-keto-PGF1α) of patients with acute cerebral infarction,so as to know the changes of platelet function on acute cerebral vascular diseases.MethodsThe level of plasma TXB2,6-keto-PGF1α and TXB2/6-keto-PGF1α(T/6-K) of 205 patients with acute cerebral infarction were measured within 24 hours and on the 7th day and 14th day,and were compared with the normal controls(n=40).ResultsThe levels of plasma TXB2,6-keto-PGF1α of patients within 24 hours,on the 7th day and 14th day were significantly higher than those in the normal controls(P<0.01).The level of plasma TXB2 was the highest on the 7th day and decreased on 14th day.The level of plasma 6-keto-PGF1α was the highest on the 14th day.T/6-K was higher than those in the normal controls within 24 hours and on the 7th day;it was highest on the 7th day and decreased on 14th day to level of the normal controls(P>0.05).ConclusionThe determine on levels of plasma TXB2,6-keto-PGF1α has important significance for knowing the changes of platelet function on acute cerebral infarction.The balance between TXB2 and 6-keto-PGF1α is important in keeping the blood smoothly.

RESUMEN

@#Objective To assess the level of plasma thromboxane B2(TXB2),6-keto-prostaglandin F1α(6-keto-PGF1α) of patients with acute cerebral infarction,so as to know the changes of platelet function on acute cerebral vascular diseases.MethodsThe level of plasma TXB2,6-keto-PGF1α and TXB2/6-keto-PGF1α(T/6-K) of 205 patients with acute cerebral infarction were measured within 24 hours and on the 7th day and 14th day,and were compared with the normal controls(n=40).ResultsThe levels of plasma TXB2,6-keto-PGF1α of patients within 24 hours,on the 7th day and 14th day were significantly higher than those in the normal controls(P<0.01).The level of plasma TXB2 was the highest on the 7th day and decreased on 14th day.The level of plasma 6-keto-PGF1α was the highest on the 14th day.T/6-K was higher than those in the normal controls within 24 hours and on the 7th day;it was highest on the 7th day and decreased on 14th day to level of the normal controls(P>0.05).ConclusionThe determine on levels of plasma TXB2,6-keto-PGF1α has important significance for knowing the changes of platelet function on acute cerebral infarction.The balance between TXB2 and 6-keto-PGF1α is important in keeping the blood smoothly.

RESUMEN

Objective To investigate the protective effect of prostaglandin E1 (PGE1) on platelet during cardiopulmonary bypass (CPB).Methods A total of 24 patients undergoing cardiac valve replacement were randomly divided into the experimental group (n=12) and the control group (n=12).The levels of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1?(6-K-PGF1?) in serum were measured at different intervals during and after CPB. The ultrastructure of platelets was also observed by transmission electron microscope.Results The levels of TXB2 and 6-K-PGF1? during CPB were significantly higher than those before CPB in the both groups(P

RESUMEN

The effects of eicosapentaenoic acid (EPA) on experimental thrombosis and its mechanism were studied. The results showed that eicosapentaenoic acid had a significant antithrombolic effect both in vivo and in vitro . EPA also had a fibrinolytic activity and can shorten euglobulin lysis time. Our studies also showed that EPA can inhibit platelet aggregation in rats. The plasma concentration of 6-keto-PGF1a and TXB2 was measured by radioimmunoassay. The results showed that EPA can reduce the contents of TXB2 and raise 6-keto-PGF1a/TXB2 ratio.

RESUMEN

Objective To study the effect and its mechanism of Modified Wenjing Decoction(Decoction for warming channels)on vasomotion in patients with menstrual disease of blood stasis due to cold accumulation.Methods The 50 patients of emmeniopathy with blood stasis due to cold accumulation were successively treated with Modified Wenjing Decoction for three menstrual cycles.The therapeutic effect and changes of nitrous oxide(NO),endothelin(ET),thromboxane B2(TXB2),6 ketone prostaglandin F1?(6-keto-PGF1?)of menstrual blood,reproductive hormones estradiol(E2)and progesterone(P)before and after treatment were observed.Results Of the 50 patients,19(38.0%)were short-term cured,24(48.0%)effective,and 7(14.0%)ineffective.After treatment,the levels of menstrual blood ET and TXB2 were decreased significantly(P

RESUMEN

Objective: To study the influences of Tiaozhixin(TZX) on NO/ET, 6 keto PGF 1a /TXB 2 and hemorrheology rats with hyperlipoidemia. Methods: The rat hyperlipoidemia and early atheroscleorsis models were established by feeding high lipid diet for 40 days. Meanwhile TZX was taken by oral administration at the dosages of 40、80g/kg. The levels of NO、ET 1、6 keto PGF 1a and TXB 2 in serum were determind, and the hemorrheology markers were observed. Results: TZX could raise the levels of NO in serumobviourly ET 1 level of the normal and model rats. The large dose of TZX could increase 6 keto PGF 1a content remarkably which benefits maintenance of the balances of 6 keto PGF 1a /TXB 2. It could lower the whole blood specific viscosity, whole blood reduction specific viscosity, plasma specific viscosity; aggregation index of RBC; shorten RBC electrophoresis time; also decrease fibrinogen content; inhibit the platelet aggregation of normal rats induced by ADP. Conclusion: TZX can improve the abnormal hemorrheology and recover the balance of TXB 2/6 keto PGF 1a and NO/ET of rats with hyperlipoidemia, which might be one of mechanisms of antiatherosclerosis action.

RESUMEN

Acute ligation of the common carotid artery on one side internal carotid artery on opposite side resulted in experimental cerebral ischemia in rabbit. Measurement of thromboxane B_2 (TXB_2) and 6-Keto-prostaglandin F_(1?)(6-Keto-PGF_(1?))in rabbit plasma were done by radioimmunoassay. Blood samples were collected from the peripheral vein and internal carotid vein. The results were as follows:1. The concentration of TXB_2 in rabbit cerebral ischemia (ligation after 30 min.) was significantly (P

RESUMEN

This paper reports the measurement of thromboxane B2 (TXB2) levels in guinea pig died of anaphylactic shock by the radioimmunassay test (RIST). 1. Statistically significant difference of plasma TXB2 level was existed between the control group (9.63?1.77ng/ml), test group before shock (9.26?3.01ng/ml) and the test group after shock (penicillin group 30.36?10.72ng/ml;serum group 40.10?6.51ng/ml) (P