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PURPOSE: As more treatments emerge for advanced, stage IV non-small-cell lung cancer (NSCLC), oncologists have difficulty predicting functional resiliency versus functional decline throughout cancer treatment. Our study evaluates functional resilience among patients with advanced NSCLC. METHODS: Functional status was evaluated through 12 months of follow-up based on disability score using the modified EQ-5D-5L (mEQ-5D-5L) survey. Participants were classified into 4 groups: functional maintenance, decline, resilient, or variable. Characteristics of 207 participants with newly diagnosed NSCLC included demographics, comorbidities, baseline Eastern Cooperative Oncology Group (ECOG) performance status (PS), mEQ-5D-5L scores, psychological symptoms, and lung cancer-specific symptoms. Treatment toxicity and grade were recorded. Resilience was defined as improvement from baseline disability scores. A 1-point increase in functional status score represents a 0.5 standard deviation change on the mEQ-5D-5L. Differences between the 4 groups were determined through Fisher's exact test or ANOVA. Kaplan-Meier curves describe overall survival (baseline through 18 months) stratified by baseline mEQ-5D-5L scores. RESULTS: Among participants, 42.0 % maintained functional status, 37.7 % experienced functional decline, 10.6 % were resilient, and 9.7 % had variable functional status. Participants with the best baseline function (score of 0) had the longest overall survival and participants with the worst baseline function (score of 5 + ) had the shortest overall survival. Among the healthiest patients, early score increases indicated shorter overall survival. Baseline ECOG PS was not associated with overall survival (p = 0.47). CONCLUSION: Baseline functional status may help better predict functional resiliency and overall survival than ECOG PS among patients receiving treatment for advanced NSCLC.

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Introduction: Preparing the school system for a future crisis requires the ability to examine the effectiveness of schools' functioning during distant learning and their level of preparedness for future crises. Functional resilience (FR) is defined as the ability to maintain vital operational continuity in the face of disturbance. The study objectives included to develop a FR index of schools and to evaluate and validate it. Methods: To enable examination of the study objectives, the study design included tool development, followed by a validation process among 20 content experts. Concurrently, an eDelphi process for building an inclusive index, based on various components of resilience was conducted. The final study tool consists of four tailored questionnaires to examine perceptions of key stakeholders, i.e.- teachers, principals, parents, and highschool students regarding communication, psychosocial aspects, perceived stress, infrastructure, resources, pedagogic support, digital literacy, and perceived FR. Using an internet panel, the tool was disseminated cross-sectionally among the four groups of stakeholders. Results: The results showed high reliability of most of the scales developed. Furthermore, a high consensus level was reached on the relative importance of each component/ stakeholder to the schools FR. The findings further suggest that there were no significant differences in the composite FR score based on characteristics such as school type/ size/geographic location. However, the findings revealed interesting variations among stakeholders, with findings suggesting greater vulnerability among some. Discussion: To increase resilience and preparedness for future adversities that school systems may face, it is recommended to periodically incorporate an assessment based on a structured tool.

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Acute environmental perturbations are reported to induce deterministic microbial community assembly, while it is hypothesized that chronic perturbations promote development of alternative stable states. Such acute or chronic perturbations strongly impact on the pre-adaptation capacity to the perturbation. To determine the importance of the level of microbial pre-adaptation and the community assembly processes following acute or chronic perturbations in the context of hydrocarbon contamination, a model system of pristine and polluted (hydrocarbon-contaminated) sediments was incubated in the absence or presence (discrete or repeated) of hydrocarbon amendment. The community structure of the pristine sediments changed significantly following acute perturbation, with selection of different phylotypes not initially detectable. Conversely, historically polluted sediments maintained the initial community structure, and the historical legacy effect of chronic pollution likely facilitated community stability. An alternative stable state was also reached in the pristine sediments following chronic perturbation, further demonstrating the existence of a legacy effect. Finally, ecosystem functional resilience was demonstrated through occurrence of hydrocarbon degradation by different communities in the tested sites, but the legacy effect of perturbation also strongly influenced the biotic response. This study therefore demonstrates the importance of perturbation chronicity on microbial community assembly processes and reveals ecosystem functional resilience following environmental perturbation.

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Pollutants are continuously released into surface waters, which decrease the dissolved oxygen (DO) concentration and leads to the formation of black-odorous water, especially in slow-flowing urban lakes and enclosed small ponds. In situ treatment by artificial aeration or water cycling, coupled with biofilm, can address this problem without occupying large amounts of land. In this study, we designed a novel sponge-based aerobic biofilm reactor (SABR) and evaluated its performance in purifying urban surface water under different conditions. In the urban lake water treatment, the continuous inflow results revealed that the NH4+-N and NO2--N concentrations in the effluent were stable and remained lower than 0.10 mg/L and 0.05 mg/L, respectively. Abrupt increases in the NH4+-N and NO2--N concentrations in the influent and sudden increases in the NH4+-N and NO2--N concentrations in the effluent were observed, and only 4 to 8 days were required for the concentrations to decline below 0.10 mg/L and 0.05 mg/L, respectively. Increases in the polyurethane sponge filling ratios in the SABRs can reduce the DO concentration but do not affect NH4+-N removal. When no biodegradable organic matter was present in the enclosed surface water, the degradation time of NH4+-N from 14.22 to 0.10 mg/L was only 9 days when SABRs were combined with water cycling, which was shorter than the time needed by water cycling alone (16 days), and most of the NH4+-N was converted to NO3--N. When massive amounts of biodegradable organic matter were present in the enclosed surface water, 22 days were required to remove the NH4+-N when SABRs were combined with water cycling. Our results indicated that organic matter could be used as a carbon source to eliminate the produced NO3--N in SABRs. Therefore, the newly developed bioreactor provides an effective approach for treating N-polluted urban surface waters.

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Climate change is expected to increase extreme weather events, such as more extreme drought and rainfall incidences, with consequences for ecosystem carbon (C) cycling. An understanding of how drying and rewetting (DRW) events affect microbe-mediated soil processes is therefore critical to the predictions of future climate. Here, a reciprocal-transplant experiment was conducted using two soils originated from distinct climate and agricultural managements to evaluate how soil biotic and abiotic properties regulate soil respiration and its resilience to simulated DRW cycles. We found that regardless of the DRW intensity, the effects of microbial community on soil respiration and its resilience to DRW cycles were dependent on soil type. Soil microbial communities yielded higher respiration rates and resilience in native than foreign soils under both one and four DRW cycles, supporting the "home-field advantage" hypothesis. Structural equation modeling demonstrated that soil pH and total C directly influenced soil respiration, but effects of soil abiotic properties on respiration resilience were mediated by microbial communities. Among microbial drivers, the microbial C utilization capacity (as characterized by community-level physiological profile, C-acquisition enzyme activities and microbial metabolic quotients) was the best predictor of respiration resilience to DRW cycles, followed by microbial biomass carbon/nitrogen ratio and microbial community composition. Our study suggests that soil microbial communities may have adapted to their historical conditions, which facilitates the resilience of soil respiration to changing environments, but this adaptation may accelerate C loss from soils facing increasingly variable climate.

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Symbiosis between prokaryotes and eukaryotes is a widespread phenomenon that has contributed to the evolution of eukaryotes. In cockroaches, two types of symbionts coexist: an endosymbiont in the fat body (Blattabacterium), and a rich gut microbiota. The transmission mode of Blattabacterium is vertical, while the gut microbiota of a new generation is mainly formed by bacterial species present in feces. We have carried out a metagenomic analysis of Blattella germanica populations, treated and non-treated with two antibiotics (vancomycin and ampicillin) over two generations to (1) determine the core of bacterial communities and potential functions of the gut microbiota and (2) to gain insights into the mechanisms of resistance and resilience of the gut microbiota. Our results indicate that the composition and functions of the bacteria were affected by treatment, more severely in the case of vancomycin. Further results demonstrated that in an untreated second-generation population that comes from antibiotic-treated first-generation, the microbiota is not yet stabilized at nymphal stages but can fully recover in adults when feces of a control population were added to the diet. This signifies the existence of a stable core in either composition and functions in lab-reared populations. The high microbiota diversity as well as the observed functional redundancy point toward the microbiota of cockroach hindguts as a robust ecosystem that can recover from perturbations, with recovery being faster when feces are added to the diet.

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There is growing recognition of the essential services provided to humanity by functionally intact ecosystems. Freshwater ecosystems are found throughout agricultural and urban landscapes and provide a wide range of ecosystem services, but globally they are also amongst the most vulnerable. In particular, ponds (lentic waters typically less than 2 ha), provide natural flood management, sequester carbon and hold significant cultural value. However, to inform their management it is important to understand (1) how functional diversity varies in response to disturbance and (2) the link between biodiversity conservation and ecosystem function. In this study, a meta-analysis of seven separate pond studies from across England and Wales was carried out to explore the effect of urban and agricultural land-use gradients, shading, emergent vegetation, surface area and pH upon groups of functionally similar members of the macroinvertebrate fauna. Functional effect groups were first identified by carrying out a hierarchical cluster analysis using body size, voltinism and feeding habits (18 categories) that are closely related to biogeochemical processes (e.g. nutrient and carbon recycling). Secondly, the influence of the gradients upon effect group membership (functional redundancy-FR) and the breadth of traits available to aid ecosystem recovery (response diversity) was assessed using species counts and functional dispersion (FDis) using 12 response traits. The effect of land-use gradients was unpredictable, whilst there was a negative response in both FR and FDis to shading and positive responses to increases in emergent vegetation cover and surface area. An inconsistent association between FDis and FR suggested that arguments for taxonomic biodiversity conservation to augment ecosystem functioning are too simplistic. Thus, a deeper understanding of the response of functional diversity to disturbance could have greater impact with decision-makers who may relate better to the loss of ecosystem function in response to environmental degradation than species loss alone.

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