RESUMEN
Bioactive polyphenols in grapes are influenced by grape variety and cultivation conditions. The Vitis labrusca L. varieties are cultivated in tropical regions and used for grape juice production. We hypothesized that polyphenols from tropical grape juices would beneficially affect redox homeostasis in humans. Therefore, the effects of acute consumption of organic and conventional grape juices from V labrusca L. on antioxidants biomarkers were investigated in healthy individuals. In a controlled, randomized, crossover, intervention trial, 24 individuals were assigned to drink 400 mL of conventional juice, organic juice, or water. Each intervention was followed by a 14-day washout period. Blood samples were obtained before and 1 hour after acute intake and analyzed for erythrocyte reduced glutathione, serum total antioxidant capacity, antioxidant enzymes in erythrocytes, and glucose and uric acid in serum. The ingestion of both grape juices resulted in elevated levels of reduced glutathione (P< .001) and serum total antioxidant capacity (P< .05) and increased activity of catalase (P< .001), superoxide dismutase (P< .001), and glutathione peroxidase (P< .05) compared with the control intervention, with no significant differences between grape juices (P< .05). The intake of juices did not affect significantly the concentrations of glucose or uric acid. Grape juice polyphenols were associated with increased antioxidants, and the chemical differences between organic and conventional juices were not predictive of the observed responses. The results suggest a bioactive potential of V labrusca L. juices to improve redox homeostasis, which is involved in defense against oxidative stress in humans.
Asunto(s)
Antioxidantes/análisis , Bebidas , Glucemia/análisis , Frutas/química , Ácido Úrico/sangre , Vitis/química , Adulto , Catalasa/sangre , Estudios Cruzados , Eritrocitos/química , Femenino , Alimentos Orgánicos , Glutatión/sangre , Glutatión Peroxidasa/sangre , Humanos , Cinética , Masculino , Persona de Mediana Edad , Oxidación-Reducción , Estrés Oxidativo/efectos de los fármacos , Superóxido Dismutasa/sangre , Clima TropicalRESUMEN
We describe a girl with Diamond-Blackfan anemia with accompanying red cell enolase deficiency. At the age of 9 yr old, the patient received allogeneic bone marrow transplantation from her HLA-identical sister who had normal red cell enolase activity. While the post transplant DNA analysis with short tandem repeat has continuously demonstrated a stable mixed chimerism on follow-up, the patient remains transfusion independent and continues to show a steady increase in red cell enolase activity for over two and a half years following bone marrow transplantation.
Asunto(s)
Anemia de Diamond-Blackfan , Trasplante de Médula Ósea , Eritrocitos/enzimología , Fosfopiruvato Hidratasa/metabolismo , Trasplante Homólogo , Anemia de Diamond-Blackfan/sangre , Anemia de Diamond-Blackfan/enzimología , Anemia de Diamond-Blackfan/cirugía , Células de la Médula Ósea/citología , Células de la Médula Ósea/metabolismo , Niño , Preescolar , Femenino , Humanos , Lactante , Fosfopiruvato Hidratasa/genéticaRESUMEN
We describe a girl with Diamond-Blackfan anemia with accompanying red cell enolase deficiency. At the age of 9 yr old, the patient received allogeneic bone marrow transplantation from her HLA-identical sister who had normal red cell enolase activity. While the post transplant DNA analysis with short tandem repeat has continuously demonstrated a stable mixed chimerism on follow-up, the patient remains transfusion independent and continues to show a steady increase in red cell enolase activity for over two and a half years following bone marrow transplantation.