RESUMEN
Obtaining water and renewable energy from the atmosphere provides a potential solution to the growing energy shortage. Leveraging the synergistic inspiration from desert beetles, cactus spines, and rice leaves, here, a multibioinspired hybrid wetting rod (HWR) is prepared through simple solution immersion and laser etching, which endows an efficient water collection from the atmosphere. Importantly, benefiting from the bionic asymmetric pattern design and the three-dimensional structure, the HWR possesses an omnidirectional fog collection with a rate of up to 23 g cm-2 h-1. We further show that the HWR could be combined with a droplet-based electricity generator to convert kinetic energy from falling droplets into electrical energy with a maximum output voltage of 200 V and a current of 2.47 µA to light up 28 LEDs. Collectively, this research provides a strategy for synchronous fog collection and power generation, which is promising for environmentally friendly energy production.
RESUMEN
To date, implant-associated infection is still a significant clinical challenge, which cannot be effectively eliminated by single therapies due to the formation of microbial biofilms. Herein, a pH-responsive nanoplatform was constructed via the in situ growth of zinc sulfide (ZnS) nanoparticles on the surface of Ti3C2 MXene nanosheets, which was subsequently introduced in poly(L-lactic acid) (PLLA) to prepare a composite bone scaffold via selective laser sintering technology. In the acidic biofilm microenvironment, the degradation of ZnS released hydrogen sulfide (H2S) gas to eliminate the biofilm extracellular DNA (eDNA), thus destroying the compactness of the biofilm. Then, the bacterial biofilm became sensitive to hyperthermia, which could be further destroyed under near-infrared light irradiation due to the excellent photothermal property of MXene, finally achieving gas/photothermal synergistic antibiofilm and efficient sterilization. The results showed that the synergistic gas/photothermal therapy for the composite scaffold not only evidently inhibited the formation of biofilms, but also effectively eradicated the eDNA of the already-formed biofilms and killed 90.4% of E. coli and 84.2% of S. aureus under near infrared light irradiation compared with single gas or photothermal therapy. In addition, the composite scaffold promoted the proliferation and osteogenic differentiation of mouse bone marrow mesenchymal stem cells. Thus, the designed scaffold with excellent biofilm elimination and osteogenesis ability has great potential as an alternative treatment for implant-associated bone infections.
Asunto(s)
Hipertermia Inducida , Nitritos , Terapia Fototérmica , Elementos de Transición , Ratones , Animales , Staphylococcus aureus , Osteogénesis , Escherichia coli , Rayos Infrarrojos , Biopelículas , Concentración de Iones de Hidrógeno , Ácido LácticoRESUMEN
Bacterial infection with high morbidity (>30%) seriously affects the defect's healing after bone transplantation. To this end, chemotherapy and photothermal therapy have been utilized for antibacterial treatment owing to their high selectivity and minimal toxicity. However, they also face several dilemmas. For example, bacterial biofilms prevented the penetration of antibacterial agents and local temperatures (over 70 °C) caused by the photothermal therapy damaged normal tissue. Herein, a co-dispersion nanosystem with chemo-photothermal function was constructed via the in situ growth of zeolitic imidazolate framework-8 (ZIF-8) on graphene oxide (GO) nanosheets. In this nanosystem, GO generates a local temperature (â¼50 °C) to increase the permeability of a bacterial biofilm under near-infrared laser irradiation. Then, Zn ions released by ZIF-8 seized this chance to react with the bacterial membrane and inactivate it, thus realizing efficient sterilization in a low-temperature environment. This antibacterial system was incorporated into a poly-l-lactic acid scaffold for bone repair. Results showed that the scaffold showed a high antibacterial rate of 85% against both Escherichia coli and Staphylococcus aureus. In vitro cell tests showed that the scaffold promoted cell proliferation.
Asunto(s)
Estructuras Metalorgánicas , Infecciones Estafilocócicas , Zeolitas , Antibacterianos/farmacología , Escherichia coli , Grafito , Humanos , Estructuras Metalorgánicas/farmacología , Terapia FototérmicaRESUMEN
Black phosphorus (BP) exhibits great potential as antibacterial materials due to its unique photocatalytic activity. However, the unsatisfactory optical absorption and quick recombination of photoinduced electron-hole pairs restrain its photocatalytic antibacterial performance. In this work, silver nanoparticles (AgNPs) were decorated on BP to construct BP@AgNPs nanohybrids and then introduced into poly-l-lactic acid scaffold. Combining the tunable bandgap of BP and the LSPR effect of AgNPs, BP@AgNPs nanohybrids displayed the broaden visible light absorption. Furthermore, AgNPs acted as electron acceptors could accelerate charge transfer and suppress electron-hole recombination. Therefore, BP@AgNPs nanohybrids achieved synergistically enhanced photocatalytic antibacterial activity under visible light irradiation. Fluorescence probe experiment verified that BP@AgNPs promoted the generation of reactive oxygen species, which could disrupt bacteria membrane, damage DNA and oxide proteins, and finally lead to bacteria apoptosis. As a result, the scaffold possessed strong antibacterial efficiency with a bactericidal rate of 97% under light irradiation. Moreover, the scaffold also exhibited good cytocompatibility. This work highlighted a new strategy to develop photocatalytic antibacterial scaffold for bone implant application.
Asunto(s)
Nanopartículas del Metal , Plata , Antibacterianos/farmacología , Luz , Pruebas de Sensibilidad Microbiana , Fósforo , Plata/farmacologíaRESUMEN
Implant-related bacterial infection is a serious complication, which even causes implant failure. Silver (Ag) nanoparticles are broadly used antibacterial agents due to their excellent antibacterial ability and broad-spectrum bactericidal property. However, the significance of burst release cannot be entirely ignored. In this study, Ag doped mesoporous bioactive glasses (Ag-MBG) nanospheres were synthesized using modified Stöber method, then incorporated into poly L-lactic acid (PLLA) matrix to prepare the composite scaffolds via selective laser sintering (SLS) technology. Herein, Mesoporous bioactive glasses (MBG) sol had many negatively-charged silicon hydroxyl groups, which could adsorb positively-charged Ag ions by electrostatic interaction and eventually form Si-O-Ag bonds into MBG. Moreover, MBG promoted osteoblast colonization due to its continuous release of Si ions. The results showed the Ag-MBG/PLLA scaffold could sustainedly release Ag ions for 28 days, and exhibited significantly antibacterial ability against Escherichia coli, its bacterial inhibition rate was over 80%. In addition, the composite scaffold also showed good cytocompatibility. It may be concluded that the prepared Ag-MBG/PLLA scaffold has great potential to repair implant-associated bone infection.
Asunto(s)
Cerámica , Plata , Antibacterianos/farmacología , Cerámica/farmacología , Escherichia coli , Plata/farmacología , Andamios del TejidoRESUMEN
Poly-L-lactic acid (PLLA) lacks osteogenic activity, which limits its application in bone repair. Zinc (Zn) is widely applied to strengthen the biological properties of polymers due to its excellent osteogenic activity. In the present study, Zn-doped mesoporous silica (Zn-MS) particles were synthesized by one-pot hydrothermal method. Then, the particles were induced into PLLA scaffolds prepared by selective laser sintering technique, aiming to improve their osteogenic activity. Our results showed that the synthesized particles possessed rosette-like morphology and uniform mesoporous structure, and the composite scaffold displayed the sustained release of Zn ion in a low concentration range, which was attributed to the shield effect of the PLLA matrix and the strong bonding interaction of Si-O-Zn. The scaffold could evidently promote osteogenesis differentiation of mouse bone marrow mesenchymal stem cells by upregulating their osteogenesis-related gene expression. Besides, Zn-MS particles could significantly increase the compressive strength of the PLLA scaffold because of their rosette-like morphology and mesoporous structure, which can form micromechanical interlocking with the PLLA matrix. The Zn-MS particles possess great potential to improve various polymer scaffold properties due to their advantageous morphology and physicochemical properties.
RESUMEN
Magnetite (Fe3O4) nanoparticles as drug carriers can achieve precise drug target due to their magnetic property. However, they are easy to aggregate in the physiological environment, which obviously limits their application in drug delivery. The development of the Fe-MIL-88B-derived method to construct the Fe3O4-loaded mesoporous carbon (Fe3O4/carbon) system is a feasible strategy to solve the issue. First, iron atoms evenly distribute in the organic links through coordination bonds in Fe-MIL-88B. After the carbonization of Fe-MIL-88B, mesoporous carbon acts as a barrier to prevent the aggregation of Fe3O4 nanoparticles. Herein, Fe-MIL-88B particles were fabricated by the hydrothermal method and then pyrolyzed to construct Fe3O4/carbon systems. Results showed that Fe3O4 nanoparticles uniformly in situ grew on mesoporous carbon generated by the carbonization of organic components. More encouragingly, the Fe3O4/carbon system loaded with DOX demonstrated pH-responsive DOX release, efficient delivery of DOX into cancer cells, and significant cancer cell killing ability. Therefore, the Fe3O4/carbon systems prepared by the Fe-MIL-88B-derived method might open up a way for targeted and controlled drug delivery.
Asunto(s)
Carbono , Nanopartículas , Portadores de Fármacos , Óxido Ferrosoférrico/química , Hierro/químicaRESUMEN
Disc gyroscope manufactured through microelectromechanical systems (MEMS) fabrication processes becomes one of the most critical solutions for achieving high performance. Some reported novel disc constructions acquire good performance in bias instability, scale factor nonlinearity, etc. However, antivibration characteristics are also important for the devices, especially in engineering applications. For multi-ring structures with central anchors, the out-of-plane motions are in the first few modes, easily excited within the vibration environment. The paper presents a multi-ring gyro with good dynamic characteristics, operating at the first resonant mode. The design helps obtain better static performance and antivibration characteristics with anchor points outside of the multi-ring resonator. According to harmonic experiments, the nearest interference mode is located at 30,311 Hz, whose frequency difference is 72.8% far away from working modes. The structures were fabricated with silicon on insulator (SOI) processes and wafer-level vacuum packaging, where the asymmetry is 780 ppm as the frequency splits. The gyro also obtains a high Q-factor. The measured value at 0.15 Pa was 162 k, which makes the structure have sizeable mechanical sensitivity and low noise.
RESUMEN
Arabidopsis heat shock factor HsfA1a is present in a latent, monomeric state under normal conditions; its activation involves heat stress-induced trimerization, binding to heat shock element in target promoters, and the acquisition of transcriptional competence. HsfA1a is an important regulator for heat stress-induced gene expression and thermotolerance. However, it is not clear whether HsfA1a is directly activated by stress and the mechanisms of the stress signaling are poorly understood. We analyzed HsfA1a activation by trimerization and DNA-binding assays in vitro and in vivo in response to heat stress, low/high pH, and hydrogen peroxide treatments. Our results show that purified recombinant HsfA1a was activated by these stress treatments in vitro. The same treatments also induced the binding to HSP18.2 and HSP70 promoters as examined by chromatin immunoprecipitation, and the HsfA1a DNA binding paralleled the mRNA expression of its target genes induced by different stresses. Stress-induced DNA-binding could be reversed, both in vitro and in vivo, by subsequent incubation with reducing agents (DTT, NADPH). These data suggest that HsfA1a can directly sense stress and become activated, and this process is dependent on the redox state. An N-terminal deletion of the amino acid residues from 48 to 74 negatively affected pH- and hydrogen peroxide-, but not heat-stress sensing.
Asunto(s)
Arabidopsis/genética , Proteínas de Unión al ADN/genética , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Proteínas de Choque Térmico/genética , Respuesta al Choque Térmico/genética , Peróxido de Hidrógeno/farmacología , Estrés Oxidativo/genética , Proteínas de Plantas/genética , Factores de Transcripción/genética , Aminoácidos , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , ADN de Plantas , Proteínas de Unión al ADN/metabolismo , Expresión Génica , Proteínas HSP70 de Choque Térmico/genética , Proteínas HSP70 de Choque Térmico/metabolismo , Factores de Transcripción del Choque Térmico , Proteínas de Choque Térmico/metabolismo , Calor , Concentración de Iones de Hidrógeno , Oxidación-Reducción , Proteínas de Plantas/metabolismo , Regiones Promotoras Genéticas , Multimerización de Proteína , ARN Mensajero/metabolismo , Proteínas Recombinantes/metabolismo , Estrés Fisiológico/genética , Factores de Transcripción/metabolismoRESUMEN
Eukaryotic cells have developed diverse strategies to combat the harmful effects of a variety of stress conditions. In the model yeast Saccharomyces cerevisiae, the increased concentration of ethanol, as the primary fermentation product, will influence the membrane fluidity and be toxic to membrane proteins, leading to cell growth inhibition and even death. Though little is known about the complex signal network responsible for alcohol stress responses in yeast cells, several mechanisms have been reported to be associated with this process, including changes in gene expression, in membrane composition, and increases in chaperone proteins that help stabilize other denatured proteins. Here, we review the recent progresses in our understanding of ethanol resistance and stress responses in yeast.
Asunto(s)
Etanol/farmacología , Saccharomyces cerevisiae/fisiología , Estrés Fisiológico/efectos de los fármacos , Aminoácidos/fisiología , Membrana Celular/efectos de los fármacos , Membrana Celular/fisiología , Tolerancia a Medicamentos , Ergosterol/metabolismo , Ácidos Grasos Insaturados/metabolismo , Proteínas Fúngicas/genética , Genes Fúngicos , Proteínas de Choque Térmico/metabolismo , Inositol/fisiología , ATPasas de Translocación de Protón/metabolismo , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/genética , Transducción de Señal/efectos de los fármacos , Transducción de Señal/genética , Transducción de Señal/fisiologíaRESUMEN
The information about DNA-binding sites of regulatory protein is important to understanding the regulatory network of DNA-protein interactions in the genome. In this report we integrated chromatin immunoprecipitation with DNA cloning to isolate genomic sites bound in vivo by heat shock factor HsfA1a in Arabidopsis thaliana. Plantlets were subjected to formaldehyde crosslinking, followed by immunoprecipitation of chromatin. The immunoprecipitated DNA was amplified by PCR and cloned. From a library enriched in putative HsfA1a-binding sites, 21 different genomic fragments were identified (65-332 bp). Six fragments contained known HsfA1a-binding motif (perfect heat shock element). Six fragments contained novel HsfA1a-binding motifs: (1) gap-type, (2) TTC-rich-type, (3) stress responsive element (STRE). Representatives of each were verified by in vitro electrophoretic mobility shift assay. About 81% of the isolated fragments contained the HsfA1a-binding motifs, and/or could be bound by HsfA1a, demonstrating that the method is efficient in the isolation of genomic binding sites of a regulatory protein. The nearest downstream genes to the HsfA1a-binding fragments, which were considered as potential HsfA1a target genes, include a set of classical heat shock protein genes: Hsp17.4, Hsp18.2, Hsp21, Hsp81-1, Hsp101, and several novel genes encoding a non-race specific disease resistance protein and a transmembrane CLPTM1 family protein.
Asunto(s)
Arabidopsis/genética , Proteínas de Unión al ADN/genética , Proteínas de Choque Térmico/genética , Proteínas de Plantas/genética , Factores de Transcripción/genética , Arabidopsis/metabolismo , Sitios de Unión , Inmunoprecipitación de Cromatina , Clonación Molecular , ADN de Plantas/genética , Proteínas de Unión al ADN/metabolismo , Ensayo de Cambio de Movilidad Electroforética , Genes de Plantas , Biblioteca Genómica , Factores de Transcripción del Choque Térmico , Proteínas de Choque Térmico/metabolismo , Respuesta al Choque Térmico/genética , Proteínas de Plantas/metabolismo , Dominios y Motivos de Interacción de Proteínas , Factores de Transcripción/metabolismoRESUMEN
Heat shock factor-DNA interaction is critical for understanding the regulatory mechanisms of stress-induced gene expression in eukaryotes. In this study, we analyzed the in vivo binding of yeast heat shock factor (HSF) to the promoters of target genes ScSSA1, ScSSA4, HSP30 and HSP104, using chromatin immunoprecipitation. Previous work suggested that yeast HSF is constitutively bound to DNA at all temperatures. Expression of HSF target genes is regulated at the post-transcriptional level. However, our results indicated that HSF does not bind to the promoters of ScSSA4 and HSP30 at normal temperature (23 degrees C). Binding to these promoters is rapidly induced by heat stress at 39 degrees C. HSF binds to ScSSA1 and HSP104 promoters under non-stress conditions, but at a low level. Heat stress rapidly leads to a notable increase in the binding of HSF to these two genes. The kinetics of the level of HSF-promoter binding correlate well with the expression of target genes, suggesting that the expression of HSF target genes is at least partially the result of HSF-promoter binding stability and subsequent transcription stimulation.
Asunto(s)
Proteínas de Unión al ADN/genética , Proteínas de Choque Térmico/genética , Estrés Oxidativo/fisiología , Proteínas de Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/fisiología , Factores de Transcripción/genética , Proteínas de Unión al ADN/metabolismo , Proteínas de Choque Térmico/metabolismo , Regiones Promotoras Genéticas/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Factores de Transcripción/metabolismoRESUMEN
Brevibacillus laterosporus is an aerobic spore-forming bacterium with the ability to produce canoe-shaped lamellar parasporal inclusions adjacent to spores. An isolate named G4 was identified as a B. laterosporus which does not produce parasporal crystals and shows significant toxic activity toward nematodes. Crude extracellular protein extract from culture supernatant of B. laterosporus G4 killed the nematodes within 12 h and finally destroyed the targets within 24 h, which suggested possible proteinaceous pathogeny. A homogeneous extracellular protease with nematicidal activities, purified by chromatography, confirmed the hypothesis that it might serve as a pathogenic factor during infection of the G4 strain. Characterization of the purified protease revealed a molecular mass of 30 kDa and optimum activity at pH 10 and 50 degrees C. The protease hydrolyzed relatively broad substrates including collagen and the cuticle of nematodes, and histopathological observations demonstrated the resulting destroyed nematode cuticle upon treatment by purified protease. Our present study reveals that extracellular protease, but not previously reported parasporal crystals, can be employed in infection against invertebrates by the B. laterosporus G4 strain.
Asunto(s)
Bacterias Grampositivas/enzimología , Bacterias Grampositivas/patogenicidad , Nematodos/microbiología , Péptido Hidrolasas/fisiología , Factores de Virulencia/fisiología , Animales , Cromatografía , Colágeno/metabolismo , Estabilidad de Enzimas , Concentración de Iones de Hidrógeno , Proteínas de Insectos/metabolismo , Nematodos/ultraestructura , Péptido Hidrolasas/aislamiento & purificación , Especificidad por Sustrato , TemperaturaRESUMEN
The temporal and spatial binding of proteins on DNA is important to the regulation of genome expression and maintenance. However, examining how the protein-DNA complexes assemble in living cells is challenging. The development of UV-crosslinking/immunoprecipitation (UV-X-ChIP) technique and the progress of its applications show the powerful potential of this method in detecting such binding behavior in vivo. UV light is a zero length crosslinker and is believed to produce less perturbation of the complex than chemical crosslinker. The use of UV laser as UV light source allows the number of photons required for crosslinking to be delivered in nano- or pico- or femtosecond intervals, extremely shortening the irradiation time and achieving higher crosslinking efficiency than conventional UV lamp, thus being well suitable for kinetic studies. UV-X-ChIP technique has been successfully applied on the study of DNA replication, transcription, chromatin structure, and genome-wide location of DNA-binding proteins.
Asunto(s)
Proteínas de Unión al ADN/metabolismo , Rayos Ultravioleta , Animales , Sitios de Unión , Reactivos de Enlaces Cruzados , Proteínas de Unión al ADN/química , Proteínas de Unión al ADN/aislamiento & purificación , Proteínas de Unión al ADN/efectos de la radiación , HumanosRESUMEN
Using UV laser cross-linking and immunoprecipitation we measured the in vivo binding of Arabidopsis heat shock transcription factor HSF1 to the promoters of target genes, Hsp18.2 and Hsp70. The amplification of promoter sequences, co-precipitated with HSF1-specific antibodies, indicated that HSF1 is not bound in the absence of heat stress. Binding to promoter sequences of target genes is rapidly induced by heat stress, continues throughout the heat treatment, and declines during subsequent recovery at room temperature. The molecular mechanisms underlying the differences between Hsp18.2 and Hsp70 in the kinetics of HSF1/promoter binding and corresponding mRNA expression profiles are discussed.
Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/genética , ADN de Plantas/metabolismo , Proteínas de Unión al ADN/metabolismo , Proteínas HSP70 de Choque Térmico/genética , Proteínas de Choque Térmico/genética , Respuesta al Choque Térmico/fisiología , Regiones Promotoras Genéticas/genética , Arabidopsis/citología , Proteínas de Arabidopsis/metabolismo , Células Cultivadas , ADN de Plantas/genética , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Genes de Plantas/genética , Factores de Transcripción del Choque Térmico , Respuesta al Choque Térmico/genética , Unión Proteica , ARN Mensajero/análisis , ARN Mensajero/genética , ARN de Planta/análisis , ARN de Planta/genética , Factores de TranscripciónRESUMEN
The molecular mechanisms underlying the initiation and maintenance of the embryonic pathway in plants are largely unknown. To obtain more insight into these processes, we used subtractive hybridization to identify genes that are upregulated during the in vitro induction of embryo development from immature pollen grains of Brassica napus (microspore embryogenesis). One of the genes identified, BABY BOOM (BBM), shows similarity to the AP2/ERF family of transcription factors and is expressed preferentially in developing embryos and seeds. Ectopic expression of BBM in Arabidopsis and Brassica led to the spontaneous formation of somatic embryos and cotyledon-like structures on seedlings. Ectopic BBM expression induced additional pleiotropic phenotypes, including neoplastic growth, hormone-free regeneration of explants, and alterations in leaf and flower morphology. The expression pattern of BBM in developing seeds combined with the BBM overexpression phenotype suggests a role for this gene in promoting cell proliferation and morphogenesis during embryogenesis.