RESUMEN

MicroRNA-449a (miR-449a) was significantly downregulated in 156 lung cancer tissues (p<0.001). We found that the low expression of miR-449a was highly correlated with cancer recurrence and survival of lung cancer patients. The transient introduction of miR-449a caused cell cycle arrest and cell senescence in A549 and 95D cells. Further studies revealed that E2F3 was a direct target of miR-449a in lung cancer cells. miR-449a also suppressed tumor formation in vivo in nude mice. These results suggest that miR-449a plays an important role in lung cancer tumorigenesis and that miR-449a might predict cancer recurrence and survival of lung cancer patients.

Asunto(s)

Factor de Transcripción E2F3/genética , Neoplasias Pulmonares/genética , MicroARNs/genética , Animales , Carcinogénesis/genética , Puntos de Control del Ciclo Celular/genética , Procesos de Crecimiento Celular/genética , Línea Celular Tumoral , Senescencia Celular/genética , Femenino , Genes Supresores de Tumor , Humanos , Neoplasias Pulmonares/patología , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Persona de Mediana Edad , ARN Interferente Pequeño/administración & dosificación , ARN Interferente Pequeño/genética , Transfección