Asunto(s)
Síndrome de Kearns-Sayre/diagnóstico , Mitocondrias Musculares , Enfermedades Neuromusculares/diagnóstico , Acidosis Láctica/diagnóstico , Adulto , Trastornos Cerebrovasculares/diagnóstico , Deficiencia de Citocromo-c Oxidasa , Diagnóstico Diferencial , Epilepsias Mioclónicas/diagnóstico , Femenino , Humanos , Masculino , Mitocondrias Musculares/ultraestructura , Músculos/patología , Examen Neurológico , SíndromeRESUMEN
The dopamine metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) and the serotonin metabolite 5-hydroxyindoleacetic acid (5-HIAA) were measured repeatedly over a period of up to 5 months at different sites of the brain ventricular system in unrestrained, awake cats. Samples of 10 microliter CSF were analyzed by high pressure liquid chromatography and subsequent electrochemical detection. Concentrations were in the range of 30-130 ng/ml for DOPAC, 110-340 ng/ml for 5-HIAA and 180-750 ng/ml for HVA. The monoamine metabolites were constant even over a period of several months if measured in the same animal but there was a marked interindividual variation. A marked gradient for monoamine metabolites was found when CSF samples from frontal sites of the lateral ventricle were compared to CSF samples from the dorsal lateral ventricle. The concentrations of DOPAC and HVA were higher at frontal sites.
Asunto(s)
Ácido 3,4-Dihidroxifenilacético/líquido cefalorraquídeo , Encéfalo/metabolismo , Ácido Homovanílico/líquido cefalorraquídeo , Ácido Hidroxiindolacético/líquido cefalorraquídeo , Fenilacetatos/líquido cefalorraquídeo , Animales , Gatos , Ventrículos Cerebrales/metabolismo , Dopamina/metabolismo , MasculinoRESUMEN
The concentrations of dopamine, noradrenaline, and dihydroxyphenylacetic acid were determined in the amygdala, neostriatum, neocortex, hippocampus, brain stem, and hypothalamus of cats, which had had 9 to 14 tonic-clonic kindled seizures. No significant biochemical changes were observed compared to control cats. Pharmacological manipulations of dopamine receptors (haloperidol, apomorphine) did not modify the kindling procedure. According to the data it seems unlikely that dopamine plays a major role in amygdaloid kindling.