RESUMEN
OBJECTIVES: To explore the gender differences in the concomitant articular injuries after acute lateral patellar dislocation (LPD). METHODS: Magnetic resonance images were prospectively analyzed in 166 patients after an acute LPD. Concomitant articular injuries included bone contusion, medial patellofemoral ligament (MPFL) injury, articular cartilage lesion, and vastus medialis obliquus (VMO) lesion. Statistical analyses were performed between the patient's gender and the incidence of concomitant articular injuries in adolescent and adult subgroups. RESULTS: The incidence of partial and complete MPFL tear in adolescent males and females were (45%, 50%) and (63.2%, 29.8%), respectively. Compared with adolescent females, adolescent males showed higher incidence of complete MPFL tear (P = 0.049). The incidence of articular cartilage lesion of patella in adolescent males and females were 40% and 21.1%, respectively. Compared with adolescent females, adolescent males showed higher incidence of articular cartilage lesion of the patella (P = 0.043). No correlations were identified in other injuries in the adolescent group. The incidence of partial and complete MPFL tear in adult males and females were (34.4%, 65.6%) and (56.8%, 37.8%), respectively. Compared with adult females, adult males showed higher incidence of complete MPFL tear (P = 0.036). The incidence of articular cartilage lesion of patella in adult males and females were 56.3% and 32.4%, respectively. Compared with adult females, adult males showed higher incidence of articular cartilage lesion of patella (P = 0.047). The incidence of VMO injury in adult males and females were 59.4% and 35.1%, respectively. Compared with adult females, adult males showed higher incidence of VMO injury (P = 0.044). No correlations were identified in other injuries in the adult group. CONCLUSIONS: Compared with females, males predispose to complete MPFL tear and articular cartilage lesion of patella after acute LPD. Compared with female adults, male adults predispose to VMO injury.
Asunto(s)
Cartílago Articular , Luxación de la Rótula , Articulación Patelofemoral , Adolescente , Adulto , Cartílago Articular/diagnóstico por imagen , Femenino , Humanos , Articulación de la Rodilla , Ligamentos Articulares/diagnóstico por imagen , Masculino , Rótula/diagnóstico por imagen , Luxación de la Rótula/diagnóstico por imagen , Luxación de la Rótula/epidemiologíaRESUMEN
OBJECTIVES: The purpose of this study was to investigate the injury characteristics of medial patellofemoral ligament (MPFL), and to analyse the correlations between the injury patterns of MPFL and articular cartilage lesions of the lateral femoral condyle in adults with acute lateral patellar dislocation (LPD). METHODS: Magnetic resonance (MR) images were prospectively obtained in 121 consecutive adults with acute LPD. Images were acquired using standardised protocols and these were independently evaluated by two radiologists. RESULTS: Forty-eight cases of partial MPFL tear and 71 cases of complete MPFL tear were identified. Injuries occurred at an isolated femoral attachment (FEM) in 48 cases, an isolated patellar insertion (PAT) in 36 cases and an isolated mid-substance (MID) in five cases. More than one site of injury to the MPFL (COM) was identified in 30 cases. The prevalence rate of chondral and osteochondral lesions of the lateral femoral condyle were 4.2% (2/48) and 6.3% (3/48) in the FEM subgroup, 19.4% (7/36) and 22.2% (8/36) in the PAT subgroup and 6.7% (2/30) and 13.3% (4/30) in the COM subgroup, respectively. The PAT subgroup showed significantly higher prevalence rate of chondral and osteochondral lesions in the lateral femoral condyle when compared with the FEM subgroup. The prevalence rate of chondral and osteochondral lesions of the lateral femoral condyle were 8.5% (6/71) and 19.7% (14/71) in the complete MPFL tear subgroup and 10.4% (5/48) and 4.2% (2/58) in the partial MPFL tear subgroup, respectively. The subgroup of the complete MPFL tear showed significantly higher prevalence rate of osteochondral lesions in the lateral femoral condyle when compared with the subgroup of the partial MPFL tear. CONCLUSIONS: Firstly, the MPFL is most easily injured at the FEM, and secondly at the PAT in adults after acute LPD. The complete MPFL tear is more often concomitant with osteochondral lesions of the lateral femoral condyle than the partial MPFL tear. The isolated patellar-sided MPFL tear is more easily concomitant with chondral lesions and osteochondral lesions of the lateral femoral condyle than the isolated femoral-sided MPFL tear.
Asunto(s)
Cartílago Articular/diagnóstico por imagen , Traumatismos de la Rodilla/diagnóstico por imagen , Ligamentos Articulares/diagnóstico por imagen , Imagen por Resonancia Magnética , Rótula/diagnóstico por imagen , Luxación de la Rótula/diagnóstico por imagen , Cartílago Articular/lesiones , Cartílago Articular/patología , Femenino , Humanos , Inestabilidad de la Articulación , Traumatismos de la Rodilla/complicaciones , Traumatismos de la Rodilla/patología , Ligamentos Articulares/lesiones , Ligamentos Articulares/patología , Imagen por Resonancia Magnética/métodos , Masculino , Rótula/lesiones , Rótula/patología , Luxación de la Rótula/diagnóstico , Prevalencia , Estudios Prospectivos , Reproducibilidad de los ResultadosRESUMEN
OBJECTIVES: The purpose of this study was to investigate the injury characteristics of medial patellofemoral ligament (MPFL), and to analyse the correlations between the injury patterns of MPFL and articular cartilage lesions of the lateral femoral condyle in children and adolescents with acute lateral patellar dislocation (LPD). METHODS: Magnetic resonance (MR) images were prospectively obtained in 127 consecutive children and adolescents with acute LPD. Images were acquired using standardised protocols and these were independently evaluated by two radiologists. RESULTS: Fifty-four cases of partial MPFL tear and 69 cases of complete MPFL tear were identified. Injuries occurred at an isolated patellar insertion (PAT) in 47 cases, an isolated femoral attachment (FEM) in 41 cases and an isolated mid-substance (MID) in four cases. More than one site of injury to the MPFL (COM) was identified in 31 cases. The prevalence rate of chondral and osteochondral lesions of the lateral femoral condyle were 23.4% (11/47) and 29.8% (14/47) in the PAT subgroup, 7.3% (3/41) and 9.8% (4/41) in the FEM subgroup and 25.8% (8/31) and 32.3% (10/31) in the COM subgroup, respectively. The PAT and COM subgroups showed significantly higher prevalence rate of chondral and osteochondral lesions in the lateral femoral condyle when compared with the FEM subgroup. The prevalence rate of chondral and osteochondral lesions of the lateral femoral condyle were 17.4% (12/69) and 30.4% (21/69) in the complete MPFL tear subgroup and 20.4% (11/54) and 13% (7/54) in the partial MPFL tear subgroup, respectively. The subgroup of the complete MPFL tear showed significantly higher prevalence rate of osteochondral lesions in the lateral femoral condyle when compared with the subgroup of the partial MPFL tear. CONCLUSIONS: Firstly, the MPFL is most easily injured at the PAT, and secondly at the FEM in children and adolescents after acute LPD. The complete MPFL tear is more often concomitant with osteochondral lesions of the lateral femoral condyle than the partial MPFL tear. The isolated patellar-sided MPFL tear and the combined MPFL tear are more easily concomitant with chondral lesions and osteochondral lesions of the lateral femoral condyle than the isolated femoral-sided MPFL tear.
Asunto(s)
Cartílago Articular/lesiones , Traumatismos de la Rodilla/diagnóstico , Ligamentos Articulares/lesiones , Imagen por Resonancia Magnética , Rótula/lesiones , Luxación de la Rótula/diagnóstico , Enfermedad Aguda , Adolescente , Cartílago Articular/patología , Niño , China/epidemiología , Femenino , Humanos , Traumatismos de la Rodilla/complicaciones , Traumatismos de la Rodilla/patología , Ligamentos Articulares/patología , Imagen por Resonancia Magnética/métodos , Masculino , Rótula/patología , Luxación de la Rótula/etiología , Prevalencia , Estudios Prospectivos , Reproducibilidad de los Resultados , RoturaRESUMEN
OBJECTIVES: The purpose of this study was to compare the diagnostic performance of high-frequency ultrasound with MR in the evaluation of medial patellofemoral ligament (MPFL) lesions after acute lateral patellar dislocation (LPD). METHODS: High-frequency ultrasound and MR images were prospectively obtained in 97 consecutive patients with acute LPD. Images were acquired using standardised protocols and were independently evaluated by two radiologists. The MPFL was assessed at three sites (patellar insertion, femoral attachment, and mid-substance) for signs of injury. RESULTS: Of a total of 291 sites in 97 MPFLs, 127 showed proven MPFL tear at surgery, including 51 sites of complete tear and 76 sites of partial tear. In a site-based analysis, the sensitivity, specificity, and accuracy of high-frequency ultrasound was 90.8%, 96.3%, and 94.6%, respectively, for partial MPFL tear and 86.3%, 96.3%, and 94%, respectively, for complete tear. For MR, the sensitivity, specificity, and accuracy was 81.6%, 95.7%, and 91.3%, respectively, for partial MPFL tear and 80.4%, 95.7%, and 92.1%, respectively, for complete tear. There was no statistical difference between high-frequency ultrasound and MR in the assessment of partial (P = 0.1, 0.777, 0.155) or complete (P = 0.425, 0.777, 0.449) MPFL lesions. Interobserver agreement was very good for high-frequency ultrasound and good for MR. CONCLUSIONS: Data suggest that high-frequency ultrasound and MR have similar diagnostic performance in the evaluation of MPFL lesions after acute LPD. KEY POINTS: ⢠High-frequency ultrasound and MR were able to detect MPFL lesions after acute lateral patellar dislocation. ⢠High-frequency ultrasound and MR showed similarly high accuracy in diagnosing MPFL lesions. ⢠Interobserver agreement was very good for high-frequency ultrasound and good for MR.
Asunto(s)
Traumatismos de la Rodilla/diagnóstico , Ligamentos Articulares/lesiones , Imagen por Resonancia Magnética/métodos , Rótula/lesiones , Luxación de la Rótula/diagnóstico , Enfermedad Aguda , Adolescente , Adulto , Niño , Femenino , Humanos , Traumatismos de la Rodilla/complicaciones , Ligamentos Articulares/diagnóstico por imagen , Ligamentos Articulares/patología , Masculino , Rótula/diagnóstico por imagen , Rótula/patología , Luxación de la Rótula/etiología , Estudios Prospectivos , Reproducibilidad de los Resultados , Rotura , Ultrasonografía , Adulto JovenRESUMEN
20(S)-protopanaxadiol (PPD(S)) and 20(R)-protopanaxadiol (PPD(R)), the main metabolites of ginsenosides Rg3(S) and Rg3(R) in black ginseng, are potential candidates for anti-cancer therapy due to their pharmacological activities such as anti-tumor properties. In the present study, we report the preparation of PPD(S, R) by a combination of steaming and biotransformation treatments from ginseng. Aspergillus niger was isolated from soil and showed a strong ability to transform Rg3(S, R) into PPD(S, R) with 100% conversion. Furthermore, the enzymatic reactions were analyzed by reversed-phase HPLC, showing the biotransformation pathways: Rg3(S)-->Rh2(S)-->PPD(S) and Rg3(R)-->Rh2(R)-->PPD(R), respectively. In addition, 12 ginsenosides including 3 pairs of epimers, namely Rg3(S), Rg3(R), Rh2(S), Rh2(R), PPD(S) and PPD(R), were simultaneously determined by reversed-phase HPLC. Our study may be highly applicable for the preparation of PPD(S) and PPD(R) for medicinal purposes and also for commercial use.
Asunto(s)
Aspergillus niger/enzimología , Ginsenósidos/química , Ginsenósidos/metabolismo , Panax/metabolismo , Sapogeninas/química , Sapogeninas/metabolismo , Antineoplásicos/química , Antineoplásicos/metabolismo , Biotransformación , Cromatografía Líquida de Alta Presión , Cromatografía de Fase Inversa , EstereoisomerismoRESUMEN
To optimize ginsenosides hydrolyzing beta-glucosidase production from Aspergillus niger, response surface methodology was carried out in two stages. The Plackett-Burman design was achieved to screen the important variables that influence beta-glucosidase production. Among 10 variables (wheat bran, soybean powder, CaCl(2), ginsenosides, KH(2)PO(4), MgSO(4), polyethylene glycol (PEG), medium volume, inoculum size, and stirring speed), it was found that wheat bran, KH(2)PO(4), and stirring speed had significant effect on beta-glucosidase activity due to very low p-values (p<0.05). Subsequently, wheat bran, KH(2)PO(4), and stirring speed were further optimized using central composite design. The optimal beta-glucosidase production was predicted to be 4650.14 U/ml with the combination of factors (wheat bran, 34.51 g/l; KH(2)PO(4), 1.78 g/l; stirring speed, 161.60 rpm/min). Finally, under optimal fermentation conditions, ginsenoside Rb(1) was converted to Rd and F(2) by A. niger within 10 min. Little compound K was detected at 30 min, and finally F(2) was completely transformed to compound K within 8 h. The putative conversion pathway of Rb(1) by A. niger was Rb(1), Rd, F(2), and compound K.
Asunto(s)
Aspergillus niger/efectos de los fármacos , Aspergillus niger/enzimología , Ginsenósidos/metabolismo , beta-Glucosidasa/biosíntesis , Cromatografía Líquida de Alta Presión , Medios de Cultivo , Interpretación Estadística de Datos , Evaluación Preclínica de Medicamentos , Espectrofotometría Ultravioleta , Estimulación Química , Triticum/químicaRESUMEN
The 1-3-2 composite is made of 1-3 composite and ceramic base. Its effective properties are calculated based on the linear piezoelectric theory and uniform field theory. The influence of piezoelectric phase volume fraction and composite aspect (thickness/width) on resonance characteristic of square 1-3-2 piezoelectric composite plate has been researched. In addition, some 1-3-2 composite samples were fabricated by dice-fill technology. The resonance frequency of samples was investigated. The results show that the experiment agrees well with the calculation. The pure thickness resonance mode of 1-3-2 composite will be gained when the volume fraction of ceramic bottom is less than 30%; that of ceramic rods is in the range of 30 approximately 80% and the ratio of thickness to width is less than 0.35.