RESUMEN
Subclinical, repeated exposures of F344 rats to sarin resulted in brain alterations in densities of chlonergic receptor subtypes that may be associated with memory loss and cognitive dysfunction. The exposures also depressed the immune system. The rat appears to be a good model for studying the effects of subclinical exposure to a nerve gas.
Asunto(s)
Sustancias para la Guerra Química/efectos adversos , Trastornos del Conocimiento/etiología , Trastornos de la Memoria/etiología , Síndrome del Golfo Pérsico , Receptores Colinérgicos/análisis , Sarín/efectos adversos , Animales , Autorradiografía , Encéfalo/efectos de los fármacos , Encéfalo/patología , Modelos Animales de Enfermedad , Sistema Inmunológico/efectos de los fármacos , Exposición por Inhalación , Ratas , Ratas Endogámicas F344 , Receptores Colinérgicos/efectos de los fármacosRESUMEN
We have previously shown that T cells from rats exposed chronically to cigarette smoke or nicotine (NT) exhibit T cell anergy and decreased proliferation to T cell mitogens. Effects of chronic NT on T cell function persist for at least 2 weeks after the termination of NT treatment. Moreover, these effects of NT are causally related to the decreased Ca(2+) response to T cell receptor (TCR) ligation and constitutive activation of protein tyrosine kinase (PTK) and phospholipase C (PLC)-gamma1 activities. Acute NT treatment also suppresses the Con A-induced T cell proliferation; however, it is not known whether the mechanism(s) by which acute and chronic NT treatments inhibit T cell proliferation are identical. To evaluate this question, LEW rats were acutely treated with NT (1 mg/kg body wt) for 1, 2, or 24 h by an ip injection or implanted with constant-release miniosmotic pumps containing saline or NT (1 mg/kg body wt/day) for a 3-week chronic exposure. Inhibition of Con A-induced proliferation of peripheral blood cells (PBC) by both acute and chronic treatments was reversed by the inhibitor of nicotinic acetylcholine receptors, mecamylamine (MEC), indicating that these receptors are required for T cell proliferation. However, the effect of acute NT on the Con A response was short lived (i.e., observed at 1 and 2 h but not at 24 h after NT administration) and was seen in PBC but not in spleen cells. Unlike the chronic treatment, acute NT administration neither suppressed significantly the TCR-mediated [Ca(2+)](i) response nor did it cause the constitutive activation of PTK and PLC-gamma1 activities in blood lymphocytes. Acute, but not chronic, NT administration increased the plasma corticosterone concentration, and this increase was also inhibited by MEC. Moreover, adrenalectomy abrogated the acute but not chronic NT effects on the Con A response. Thus, the acute and chronic effects of NT on T lymphocytes are mechanistically distinct phenomena. Whereas chronic administration of NT causes T cell anergy, acute effects are primarily mediated via the activation of the hypothalamus-pituitary-adrenal axis.
Asunto(s)
Sistema Inmunológico/efectos de los fármacos , Nicotina/toxicidad , Adrenalectomía , Animales , Calcio/metabolismo , Concanavalina A/farmacología , Corticosterona/sangre , Sistema Hipotálamo-Hipofisario/efectos de los fármacos , Activación de Linfocitos/efectos de los fármacos , Masculino , Sistema Hipófiso-Suprarrenal/efectos de los fármacos , Proteínas Tirosina Quinasas/metabolismo , Ratas , Ratas Endogámicas LewRESUMEN
Chronic exposure of mice and rats to cigarette smoke affects T-cell responsiveness that may account for the decreased T-cell proliferative and T-dependent antibody responses in humans and animals exposed to cigarette smoke. However, the mechanism by which cigarette smoke affects the T cell function is not clearly understood. Our laboratory has shown that chronic exposure of rats to nicotine inhibits the antibody-forming cell response, impairs the antigen-mediated signaling in T cells, and induces T cell anergy. To determine the mechanism of cigarette smoke-induced immunosuppression and to compare it with chronic nicotine exposure, rats were exposed to diluted, mainstream cigarette smoke for up to 30 months or to nicotine (1 mg/kg b.wt./24 h) via miniosmotic pumps for 4 weeks, and evaluated for immunological function in vivo and in vitro. This article presents evidence suggesting that T cells from long-term cigarette smoke-exposed rats exhibit decreased antigen-mediated proliferation and constitutive activation of protein tyrosine kinase and phospholipase C-gamma1 activities. Moreover, spleen cells from smoke-exposed and nicotine-treated animals have depleted inositol-1, 4,5-trisphosphate-sensitive Ca(2+) stores and a decreased ability to raise intracellular Ca(2+) levels in response to T cell antigen receptor ligation. These results suggest that chronic smoking causes T cell anergy by impairing the antigen receptor-mediated signal transduction pathways and depleting the inositol-1,4, 5-trisphosphate-sensitive Ca(2+) stores. Moreover, nicotine may account for or contribute to the immunosuppressive properties of cigarette smoke.
Asunto(s)
Calcio/metabolismo , Inmunidad/efectos de los fármacos , Inositol 1,4,5-Trifosfato/fisiología , Nicotiana , Plantas Tóxicas , Transducción de Señal/inmunología , Humo/efectos adversos , Linfocitos T/inmunología , Animales , Células Productoras de Anticuerpos/efectos de los fármacos , Antígenos/inmunología , Cotinina/sangre , Activación Enzimática/efectos de los fármacos , Femenino , Estado de Salud , Isoenzimas/metabolismo , Nicotina/farmacología , Agonistas Nicotínicos/farmacología , Fosfolipasa C gamma , Proteínas Tirosina Quinasas/metabolismo , Ratas , Ratas Endogámicas F344 , Complejo Receptor-CD3 del Antígeno de Linfocito T/efectos de los fármacos , Complejo Receptor-CD3 del Antígeno de Linfocito T/inmunología , Transducción de Señal/efectos de los fármacos , Bazo/citología , Bazo/efectos de los fármacos , Bazo/inmunología , Linfocitos T/efectos de los fármacos , Fosfolipasas de Tipo C/metabolismoRESUMEN
We have studied the in vitro effects of lead (Pb) as Pb-acetate (0. 1-1000 ppm) on the activation of rat spleen (SP) cells. At a concentration of 0.5 to 200 ppm, Pb augmented the uptake of [3H]thymidine, progression of SP cells through the cell cycle, and allogeneic and syngeneic mixed lymphocyte reactions. However, at concentrations above 200 ppm, Pb inhibited the proliferation of these cells. To understand the cellular and molecular basis of these responses, we examined the effects of Pb on the proliferation of isolated T and/or B cell populations. Pb failed to stimulate the proliferation of isolated T and B cells; however, the addition of gamma-irradiated B cells to T cell cultures or irradiated T cells to B cell cultures resulted in Pb-induced incorporation of [3H]thymidine. On the other hand, macrophages were unable to reconstitute this response. Pb also induced a significant rise in the intracellular concentration of inositol 1,4,5-trisphosphate in SP cells; however, unlike the activation of lymphocytes through the antigen receptors, Pb did not significantly stimulate protein tyrosine kinase activity. These observations suggest that Pb facilitates the T cell-B cell interaction-dependent proliferation of lymphocytes through a signaling pathway(s) independent of the antigen receptor.
Asunto(s)
Linfocitos B/efectos de los fármacos , Comunicación Celular/efectos de los fármacos , Plomo/farmacología , Activación de Linfocitos/efectos de los fármacos , Linfocitos T/efectos de los fármacos , Animales , Linfocitos B/citología , Linfocitos B/inmunología , Ciclo Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Inositol 1,4,5-Trifosfato/metabolismo , Activación de Linfocitos/fisiología , Prueba de Cultivo Mixto de Linfocitos , Masculino , Proteínas Tirosina Quinasas/metabolismo , Ratas , Ratas Endogámicas , Bazo/citología , Bazo/efectos de los fármacos , Bazo/metabolismo , Estimulación Química , Linfocitos T/citología , Linfocitos T/inmunologíaRESUMEN
Tobacco smoking may predispose humans to respiratory disease, and may be a compounding risk factor in HIV infection and progression to AIDS. We have demonstrated that chronic exposure of mice and rats to cigarette smoke or nicotine inhibits T cell responsiveness, which may account for the decreased antibody response to T-dependent antigens seen in these animals. This inhibition may result from aberrant antigen-mediated signaling and depletion of IP3-sensitive Ca2+ stores in nicotine-treated animals. Moreover, nicotine appears to moderate the inflammation associated with turpentine-induced sterile abscess and influenza infection. These anti-inflammatory properties of nicotine may account for longer survival of nicotine-treated than control mice lethally infected with influenza virus. However, because inflammation is required for clearance of many pathogens, nicotine-treated mice exhibit significantly higher titers of influenza virus following infection. These results offer an explanation for the higher susceptibility to some infectious diseases, but greater resistance to some inflammatory diseases among human smokers.
Asunto(s)
Inmunosupresores/farmacología , Nicotina/farmacología , Receptores de Antígenos de Linfocitos T/efectos de los fármacos , Linfocitos T/efectos de los fármacos , Absceso/inducido químicamente , Absceso/inmunología , Animales , Calcio/metabolismo , Fiebre/etiología , Virus de la Influenza A/efectos de los fármacos , Inositol 1,4,5-Trifosfato/metabolismo , Ratones , Infecciones por Orthomyxoviridae/inmunología , Neumonía Viral/inmunología , Ratas , Ratas Endogámicas Lew , Receptores de Antígenos de Linfocitos T/inmunología , Receptores de Antígenos de Linfocitos T/metabolismo , Linfocitos T/fisiología , TrementinaRESUMEN
Nicotine (NT) treatment impairs T-cell receptor (TCR)-mediated signaling, leading to the arrest of T cells in the G1 phase of the cell cycle and inhibition of the antibody plaque-forming cell (AFC) response to sheep red blood cells (SRBC). This paper summarizes some of the previous findings related to cigarette smoke/NT and the immune response, and presents preliminary evidence suggesting that mice chronically treated with NT (0.5 mg/day/kg body weight) have a depressed inflammatory response in the turpentine-induced abscess model of inflammation. This ability of nicotine to attenuate an inflammatory response may also be the cause of reduced mortality of chronically nicotine-treated mice from acute influenza A pneumonitis. Moreover, in LEW rats, decreased anti-SRBC AFC responses were also observed after intracerebroventricular (i.c.v.) administration of relatively small concentrations of NT (28 micrograms/day/kg body weight) which, when given peripherally, did not affect the AFC response. In vitro the addition of NT to T cells increased protein tyrosine kinase (PTK) activity and intracellular Ca2+ concentration [Ca2+]i. These results support the hypothesis that NT alters immune responses by directly interacting with T cells, as well as indirectly through brain-immune interactions.
Asunto(s)
Sistema Inmunológico/efectos de los fármacos , Inmunidad/efectos de los fármacos , Nicotina/farmacología , Agonistas Nicotínicos/farmacología , Absceso/tratamiento farmacológico , Animales , Antiinflamatorios no Esteroideos/farmacología , Temperatura Corporal/efectos de los fármacos , Calcio/metabolismo , Técnica de Placa Hemolítica , Virus de la Influenza A , Inyecciones Intraventriculares , Masculino , Ratones , Actividad Motora/efectos de los fármacos , Nicotina/administración & dosificación , Agonistas Nicotínicos/administración & dosificación , Infecciones por Orthomyxoviridae/metabolismo , Infecciones por Orthomyxoviridae/fisiopatología , Proteínas Tirosina Quinasas/metabolismo , Ratas , Ratas Endogámicas Lew , Linfocitos T/efectos de los fármacos , Linfocitos T/inmunología , Linfocitos T/metabolismoRESUMEN
Cigarette smoke is a major health risk factor which significantly increases the incidence of diseases including lung cancer and respiratory infections. This increased susceptibility may result from cigarette smoke-induced impairment of the immune system. While the acute effects of cigarette smoke on the immune system are less clear, chronic exposure to cigarette smoke or nicotine causes T cell unresponsiveness. This apparent T cell anergy may account for or contribute to the immunosuppressive and anti-inflammatory properties of cigarette smoke/nicotine. Nicotine-induced immunosuppression may result from its direct effects on lymphocytes, indirectly through its effects on the neuroendocrine system, or both.
Asunto(s)
Neuroinmunomodulación/efectos de los fármacos , Nicotina/inmunología , Agonistas Nicotínicos/inmunología , Fumar/inmunología , Humanos , Neuroinmunomodulación/inmunologíaRESUMEN
Previously, we have shown that both T and B lymphocytes from chronically nicotine-treated (NT) animals exhibit tolerance to activation by Ags (ligation of Ag receptors), as indicated by their decreased ability to mobilize intracellular calcium and, at least in T cells, arrest of cells in the G0/G1 phase of the cell cycle. Herein, we demonstrate that NT T cells significantly lose their ability to up-regulate inositol trisphosphate synthesis in response to TCR ligation or nonspecific activation of G proteins by AIF-4. However, increases in cAMP concentrations of T cells following activation of G protein-sensitive adenylate cyclase by cholera or pertussis toxin were not significantly affected by the nicotine treatment. Interestingly, compared with control T cells, the background levels of inositol trisphosphate were significantly elevated in NT T cells, indicating some degree of activation in these cells. This inference was further supported by observations that naive T cells from NT animals exhibit tyrosine phosphorylation of several substrates, including phospholipase C-gamma1, which were either absent or underphosphorylated in unstimulated control T cells. Moreover, when, after 4-wk nicotine treatment, nicotine pumps were removed and serum cotinine levels fell to background, inhibition of the Ab-forming cells and Ca2+ responses continued for at least 2 more wk. These results suggest that chronic in vivo nicotine exposure leads to T cell anergy and may contribute to nicotine/cigarette smoke-induced immunosuppression.
Asunto(s)
Tolerancia Inmunológica/efectos de los fármacos , Nicotina/farmacología , Linfocitos T/efectos de los fármacos , Linfocitos T/inmunología , Compuestos de Aluminio/farmacología , Animales , Formación de Anticuerpos , Antígenos/administración & dosificación , Calcio/metabolismo , Eritrocitos/inmunología , Fluoruros/farmacología , Humanos , Inositol 1,4,5-Trifosfato/metabolismo , Isoenzimas/metabolismo , Masculino , Nicotina/administración & dosificación , Fosfolipasa C gamma , Fosforilación , Proteínas Tirosina Quinasas/metabolismo , Ratas , Ratas Endogámicas Lew , Receptores de Antígenos de Linfocitos T/metabolismo , Ovinos , Transducción de Señal , Fumar/efectos adversos , Fumar/inmunología , Linfocitos T/metabolismo , Fosfolipasas de Tipo C/metabolismo , Tirosina/metabolismoRESUMEN
Previous work has demonstrated that chronic exposure of rats to cigarette smoke causes inhibition of the antibody-forming cell (AFC) response and that the particulate phase of cigarette smoke, containing most of the nicotine in cigarette smoke, is essential for immunosuppression. Using intradermally implanted miniosmotic pumps, LEW rats were exposed to nicotine or its principal metabolite, cotinine, at the rate of about 14 micrograms/hr for 3-4 weeks. Serum cotinine levels in nicotine-treated (NT) animals of 219 +/- 40 ng/ml (on Day 10) were comparable to average human smokers. No significant differences between control (CON) and NT animals were observed in the distribution of lymphocyte subsets. However, nicotine, but not cotinine, treatment for 3 to 4 weeks inhibited both the T-dependent and T-independent AFC responses and proliferation to anti-CD3. Con A response was observed in 4-week but not in 3-week NT animals. Cell cycle analysis revealed that upon stimulation with Con A or anti-CD3, in spite of comparable surface expression of IL-2 receptors and class II MHC molecules, significantly fewer NT T cells entered the S and G2/M phases than CON T cells, indicating an arrest in the G0/G1 phase. Furthermore, B and T cells from NT animals were unable to elevate the intracellular calcium levels normally in response to ligation of antigen receptors, although Ca2+ responses of salivary gland cells to acetylcholine were normal. Thus, nicotine may significantly contribute to the immunosuppressive effects of chronic smoking by inducing a state of anergy in lymphocytes and may be related to their impaired response to antigen-induced signaling.
Asunto(s)
Linfocitos B/efectos de los fármacos , Nicotina/toxicidad , Transducción de Señal/efectos de los fármacos , Linfocitos T/efectos de los fármacos , Animales , Células Productoras de Anticuerpos/inmunología , Linfocitos B/inmunología , Linfocitos B/metabolismo , Calcio/análisis , Ciclo Celular/efectos de los fármacos , Cotinina/toxicidad , Genisteína , Antígenos de Histocompatibilidad Clase II/análisis , Isoflavonas/farmacología , Activación de Linfocitos/efectos de los fármacos , Prueba de Cultivo Mixto de Linfocitos , Subgrupos Linfocitarios/efectos de los fármacos , Subgrupos Linfocitarios/inmunología , Masculino , Ratas , Ratas Endogámicas Lew , Receptores de Antígenos/fisiología , Receptores de Interleucina-2/análisis , Organismos Libres de Patógenos Específicos , Linfocitos T/inmunología , Linfocitos T/metabolismoRESUMEN
Signaling between intracellular Ca2+ stores and cell membrane channels or transporters is important to Ca(2+)-based second messenger systems. Two hypotheses, the capacitative and the Ca(2+)-induced Ca(2+)-influx models have been proposed to explain aspects of this signaling. In this study, we examined the applicability of these models in neuroendocrine (PC12), neuronal (dorsal root ganglion), immune (spleen), and fibroblast (3T3) cells. We used thapsigargin (TPG) to deplete specific intracellular Ca2+ stores and to increase the cytoplasmic Ca2+ concentration ([Ca2+]), and Ca2+ free medium to prevent Ca2+ influx and lower cytoplasmic [Ca2+]. We demonstrate that, although TPG causes an increase of [Ca2+]i in all cells examined, the subsequent stimulation of Ca2+ influx varies from high in spleen, to moderate in 3T3 and PC12, to undetectable in DRG cells. All cell types exhibited Ca2+ influx when Ca2+ was added to the medium following an exposure to Ca(2+)-free medium. Without added provisions, the two aforementioned hypotheses are inadequate in explaining the TPG-induced Ca(2+)-influx in all cell types. These results support the hypothesis of the existence of unique Ca2+ channels or transporters in spleen cells that operate subsequent to TPG treatment and are distinct from the voltage-gated Ca2+ channels and Ca(2+)-activated non-selective cation channels present in excitable cells.
Asunto(s)
Células 3T3/efectos de los fármacos , Calcio/fisiología , Neuronas/efectos de los fármacos , Células PC12/efectos de los fármacos , Sistemas de Mensajero Secundario/efectos de los fármacos , Bazo/efectos de los fármacos , Terpenos/farmacología , Células 3T3/fisiología , Animales , ATPasas Transportadoras de Calcio/antagonistas & inhibidores , Compartimento Celular/efectos de los fármacos , Embrión de Pollo , Retículo Endoplásmico/efectos de los fármacos , Retículo Endoplásmico/enzimología , Ganglios Espinales/citología , Ganglios Espinales/efectos de los fármacos , Líquido Intracelular/efectos de los fármacos , Líquido Intracelular/metabolismo , Masculino , Ratones , Modelos Biológicos , Neuronas/fisiología , Especificidad de Órganos , Células PC12/fisiología , Ratas , Ratas Endogámicas F344 , Bazo/citología , TapsigarginaRESUMEN
Experimental evidence on the immunomodulating effects of ethanol is contradictory and, in animals, the immunological effects of long-term alcohol intake may depend on the age of animal, amount of alcohol consumed, and nutritional composition of the administered diet. In this study, immunological effects of pair-feeding a 35% ethanol-containing Bio-Serv liquid diet for 6 weeks were evaluated using two major histocompatibility complex (MHC)-compatible inbred strains of rats (F344 and LEW). Food intake, rate of gain in body weight, and percentages of B cells, T cells, and T cell subtypes were not affected by ethanol intake. Also, proliferative responses of lymphocytes to T and B cell mitogens were similar in control and ethanol-fed groups of the two strains. Ethanol consumption had no significant influence on spleen weights and the antibody plaque-forming cell (PFC) response in F344 rats; however, in LEW rats, ethanol ingestion leads to a significant decrease (about 16%; p < 0.012) in spleen weight and a > 75% reduction in the PFC response. These results suggest that a non-MHC-encoded gene(s) regulates the ethanol-mediated immunosuppression of the PFC response. Thus, LEW-F344 combination may provide an excellent model to characterize genetic factors which determine sensitivity/resistance to immunological effects of ethanol ingestion.
Asunto(s)
Etanol/toxicidad , Genes , Tolerancia Inmunológica/efectos de los fármacos , Bazo/efectos de los fármacos , Consumo de Bebidas Alcohólicas , Animales , Formación de Anticuerpos/efectos de los fármacos , Linfocitos B/efectos de los fármacos , Linfocitos B/inmunología , Corticosterona/sangre , Ingestión de Alimentos/efectos de los fármacos , Femenino , Hígado/efectos de los fármacos , Activación de Linfocitos/efectos de los fármacos , Complejo Mayor de Histocompatibilidad , Masculino , Ratas , Ratas Endogámicas F344 , Ratas Endogámicas Lew , Bazo/citología , Linfocitos T/efectos de los fármacos , Linfocitos T/inmunología , Aumento de Peso/efectos de los fármacosRESUMEN
The murine acquired immunodeficiency syndrome (MAIDS) is caused in susceptible C57BL/6 (B6) mice by a defective murine leukemia virus (MuLV) and resembles human AIDS in several respects. The disease is characterized by hypergammaglobulinemia, polyclonal B cell activation, lymphadenopathy, and generalized immunosuppression within 5-8 weeks postinfection. The virus has been shown to infect B cells and macrophages and both T and B cells are required for MAIDS development. The manner in which T cells contribute to the disease process is not known. We report here that this retroviral infection leads to induction of a Thy-CD4+T cell subpopulation capable of transferring all the symptoms of MAIDS disease to normal B6 and B6 nu/nu. Essentially 100% of T cells recovered from B6 nu/nu mice, injected with CD4+ T cells from B6 MAIDS animal, is of the Thy-CD4+ phenotype. The proliferation of these T cells in culture and their ability to cause MAIDS in SCID mice is totally dependent on the presence of B cells. These T cells do not exhibit significant V beta restriction of their T cell receptors (TCR) and, by PCR analysis, have defective virus-specific sequences in the cellular genome. By several criteria, however, these cells do not produce the infectious virus. These results suggest that a B-cell-dependent population of CD4+ T cells from MAIDS animals, in the absence of detectable infectious virus production, has the ability to transfer MAIDS-like disease.
Asunto(s)
Linfocitos T CD4-Positivos/microbiología , Síndrome de Inmunodeficiencia Adquirida del Murino/etiología , Síndrome de Inmunodeficiencia Adquirida/etiología , Animales , Linfocitos B/inmunología , Secuencia de Bases , Linfocitos T CD4-Positivos/inmunología , Cartilla de ADN/genética , Virus Defectuosos/genética , Humanos , Virus de la Leucemia Murina/genética , Activación de Linfocitos , Cooperación Linfocítica , Ratones , Ratones Endogámicos C57BL , Ratones Desnudos , Ratones SCID , Datos de Secuencia Molecular , Síndrome de Inmunodeficiencia Adquirida del Murino/inmunología , Síndrome de Inmunodeficiencia Adquirida del Murino/microbiologíaRESUMEN
Rat autoreactive T cells (ATs) recognize a membrane component(s) on syngeneic B cells in association with class II MHC antigens resulting in proliferation of ATs as well activation and differentiation of B cells. Results presented herein indicate that ATs recognize a stimulating antigen(s) SA, in association with class II MHC antigens, on the B cell surface. Our studies using inhibitors of carbohydrate and protein synthesis suggest that SA is a glycoprotein(s) with a high turnover rate but is not an immunoglobulin. Treatment of B cells with mannosidase abrogates their ability to stimulate AT proliferation. Furthermore, pretreatment of B cells with GNA (a lectin from Galanthus nivalis that reacts with free terminal-mannose residues on glycoconjugates) also inhibits their ability to stimulate ATs. However, these treatments do not affect the competence of B cells to stimulate an allogeneic MLR or present a conventional antigen to T cells. The frequency of CD4+ T cells proliferating in response to syngeneic B cells is very high (0.2-0.5%) and is in line with frequencies seen in "superantigen"-type responses. Moreover, T cell receptors expressed on ATs use mainly V beta 6, V beta 11, and V beta 8 regions. Based on these data, SA appears to be a fast turnover, terminal mannose-containing, superantigen-like glycoprotein on the B cell surface.
Asunto(s)
Autoantígenos/inmunología , Linfocitos B/inmunología , Glicoproteínas/inmunología , Manosa/análisis , Linfocitos T/inmunología , Animales , Autoantígenos/química , Linfocitos B/efectos de los fármacos , Galanthus , Glicoproteínas/química , Activación de Linfocitos/efectos de los fármacos , Activación de Linfocitos/inmunología , Manosa/inmunología , Manosidasas/farmacología , Ratas , Ratas Endogámicas ACI , Ratas Endogámicas F344RESUMEN
We have previously reported that chronic exposure of rats to cigarette smoke inhibits the antibody-forming cell (AFC) response to both T-dependent and T-independent antigens and may reflect B cell dysfunction. In this communication we extend these studies to show that T cell functions are normal in chronically smoke-exposed rats (SM) as judged by their responses to mitogens and "nominal" or alloantigens. While B cells from SM respond significantly to the B cell mitogen lipopolysaccharide (LPS), they fail to proliferate in response to anti-IgM (anti-mu) or to produce significant AFC response to sheep red blood cells. On the basis of the number of rosettes formed with trinitrophenylated (TNP) horse red blood cells; the frequency of TNP-binding cells (TNP-ABC) in the spleens of SM is comparable to sham control rats. However, the proliferation of TNP-ABC to TNP-Brucella abortus is significantly decreased in SM. These differences in SM B cell responses, i.e., between LPS and anti-mu/antigen, may to be related to the ability of LPS to bypass a portion of the membrane signal transduction cascade. These results suggest that cigarette smoke affects an early step(s) in the antigen-dependent B cell signal transduction pathway.
Asunto(s)
Linfocitos B/inmunología , Inmunidad Innata , Fumar/efectos adversos , Animales , Antígenos de Superficie/inmunología , Linfocitos B/efectos de los fármacos , División Celular , Membrana Celular/inmunología , Activación de Linfocitos/efectos de los fármacos , Masculino , Mitógenos/farmacología , Ratas , Ratas Endogámicas F344 , Linfocitos T/efectos de los fármacos , Linfocitos T/inmunologíaRESUMEN
Evidence has been presented to show that CD4+ autoreactive T cell lines (ATs)2 in the rat require periodic stimulation with syngeneic spleen cells for in vitro proliferation. This proliferation can be blocked by treatment of the stimulator (spleen) cells with mAb to Ia antigens. Although ATs are Ia+ and can activate the allogeneic MLR, they fail to be autostimulatory. Fractionation of the spleen cells revealed that ATs can be stimulated with B cells and not by macrophages, although the latter were efficient in several accessory cell functions, including antigen presentation, lectin-dependent T cell activation and allogenic MLR response. Moreover, B cells proliferated and differentiated in response to AT cells. These data are compatible with a model in which ATs respond to hitherto undetermined B cell membrane antigen(s) in association with MHC class II antigens. These results may have important implications in understanding autoimmune responses.
Asunto(s)
Autoinmunidad , Linfocitos B/inmunología , Activación de Linfocitos , Linfocitos T/inmunología , Animales , Formación de Anticuerpos , Antígenos de Diferenciación de Linfocitos T/inmunología , Linfocitos T CD4-Positivos/inmunología , Línea Celular , Antígenos de Histocompatibilidad Clase II/inmunología , Técnicas In Vitro , Prueba de Cultivo Mixto de Linfocitos , Ratas , Ratas EndogámicasRESUMEN
The formation of DNA adducts in the nasal, lung, and liver tissues of rats exposed daily to fresh smoke from a University of Kentucky reference cigarette (2R1) for up to 40 weeks was examined. The amount of smoke total particulate matter (TPM) inhaled and the blood carboxyhemoglobin (COHb) values averaged 5-5.5 mg smoke TPM/day/rat and 5.5%, respectively. The pulmonary AHH activity measured at the termination of each experiment showed an average increase of about two- to threefold in the smoke-exposed groups. These observations suggested that animals effectively inhaled both gaseous and particulate phase constituents of cigarette smoke. DNAs from nasal, lung, and liver tissue were extracted and analyzed by an improved 32P-postlabeling procedure. The results showed that the mainstream cigarette smoke induced a spectrum of at least four new DNA adducts in the nasal mucosa of the exposed rats and the magnitude of these adducts increased with the duration of exposure. In the lung tissue, the smoke exposure induced an accumulation of one DNA adduct, which upon cessation of exposure for 19 weeks was reduced by about 75%. Smoke-related adducts were not detected in the liver, a nontarget tissue. Selective chromatography and butanol extractability suggested that the nasal and lung DNA adducts are aromatic and/or hydrophobic in nature and that the smoke-related lung DNA-adduct may contain polar group(s). These data demonstrate the DNA-damaging potential of long term fresh cigarette smoke exposure and suggest the ability of the tissue to partially recover from such damage following cessation of the exposure.
Asunto(s)
ADN/metabolismo , Pulmón/metabolismo , Mucosa Nasal/metabolismo , Nicotiana , Plantas Tóxicas , Humo , Envejecimiento , Animales , Hígado/metabolismo , Masculino , Ratas , Ratas EndogámicasRESUMEN
Chronic inhalation of cigarette smoke in rats preferentially inhibited the plaque-forming cell (PFC) response of lung-associated lymph nodes (LALN) to sheep red blood cells (SRBC), compared to anatomically distant lymph nodes. Inhibition of the antibody response in LALN of smoke-exposed animals was first detected at 21 weeks of smoke inhalation and was well established by the 27th week of smoke exposure. After prolonged exposure (greater than 34 weeks) to cigarette smoke, similar smoke-induced changes in PFC response took place in other lymphoid tissues as well. Cigarette smoke affected the response of LALN cells to a T cell-dependent antigen (SRBC). Exposure to cigarette smoke, however, did not alter the relative percentages of W3/13-positive (T cells) or Ig-positive (B cells) cells, nor did it alter the relative percentages of T cell subsets as scored by their surface phenotypes, i.e., T helper (W3/25+) or T suppressor/cytotoxic (OX-8+) cells. The percentage of phagocytic cells and the accessory cell functions of macrophages remained comparable between sham and smoke-exposed animals. Exposure to cigarette smoke did not significantly alter the response of LALN cells to T cell mitogens (concanavalin A and phytohemagglutinin). However, response to a T cell-independent antigen trinitrophenyl Brucella abortus was also significantly reduced. These results show that cigarette exposure in the rat results in a decreased antibody response and this exposure to cigarette smoke may primarily affect the B cell function.
Asunto(s)
Formación de Anticuerpos , Nicotiana , Plantas Tóxicas , Humo/efectos adversos , Animales , Antígenos/administración & dosificación , Pulmón/inmunología , Ganglios Linfáticos/inmunología , Activación de Linfocitos , Tejido Linfoide/inmunología , Macrófagos/fisiología , Ratas , Ratas EndogámicasRESUMEN
Highly purified rat Ia-negative (OX-6-) and Ia-positive (OX-6+) T cells were employed to examine the requirement for accessory cells (AC) and/or soluble factors in the activation of resting T cells with Con A, PHA, sodium periodate, or antigen. A variety of cells were employed as AC, including Ia-positive and Ia-negative macrophages (M phi), gamma-irradiated (2000 rad) or non-irradiated OX-6+ T cells, and several Ia-negative adenovirus-transformed rat embryo fibroblast cell lines. Our results suggested that for the expression of IL-2 receptors (IL-2R) and proliferation of OX-6- T cells in response to Con A, PHA, or antigen, there was an obligatory requirement for the presence of AC which could not be overcome by the addition of IL-1 and/or IL-2. Activation of OX-6- T cells with antigen required the presence of Ia+ AC, while activation with mitogens could be initiated with Ia- AC. M phi were efficient in AC function in all responses tested, while the AC function of OX-6+ T cells (TAPC) proved discriminatory under different conditions. The optimal response to PHA required much higher concentrations of TAPC as AC than for the Con A response. TAPC failed to stimulate sodium periodate-treated T cells under any conditions tested. Furthermore, when TAPC were employed as AC, their antigen-presenting ability was radiosensitive, while their AC function for Con A and PHA was radioresistant. These results suggest that molecules involved in T cell-AC interactions may differ, depending on the source of AC and/or type of the proliferative stimulus provided to T cells. This data has been discussed in the context of T-cell activation.
Asunto(s)
Células Presentadoras de Antígenos/fisiología , Activación de Linfocitos , Linfocitos T/inmunología , Animales , Concanavalina A/farmacología , Antígenos de Histocompatibilidad Clase II/inmunología , Interleucina-1/farmacología , Interleucina-2/farmacología , Macrófagos/inmunología , Ácido Peryódico/farmacología , Fitohemaglutininas/farmacología , Ratas , Ratas Endogámicas F344 , Receptores Inmunológicos/fisiología , Receptores de Interleucina-2 , Linfocitos T/efectos de los fármacos , Linfocitos T/ultraestructuraRESUMEN
Eleven adult male stumptailed monkeys (Macaca arctoides) were chronically exposed to either a low dose (human equivalent of 1 pack/day) or a high dose (human equivalent of 3 packs/day) of high-tar, high-nicotine University of Kentucky reference cigarette smoke for 4-8 years. Several parameters of their immunological response were compared to six nonsmoked control animals. The results from these experiments suggest that cigarette smoking does not significantly affect the response of spleen cells to the mitogens phytohemagglutinin or lipopolysaccharide. However, spleen cells from animals subjected to the heavy dose of cigarette smoke demonstrated a significant reduction in their natural killer cell-mediated lytic activity and a decreased response to concanavalin A. These results suggest that cigarette smoking may have a differential effect on lymphocyte subpopulations, and that the effects on the immune response are related to the dose of cigarette smoke.
Asunto(s)
Inmunidad Innata , Células Asesinas Naturales/inmunología , Contaminación por Humo de Tabaco/efectos adversos , Animales , Hidrocarburo de Aril Hidroxilasas/metabolismo , Activación de Linfocitos , Macaca , Masculino , Plantas Tóxicas , Bazo/citología , Bazo/inmunología , Distribución Tisular , NicotianaRESUMEN
We have examined the characteristics of a rat suppressor T cell (Ts) that inhibited the antigen-dependent proliferative response of antigen-primed T cells. The kinetics of in vitro induction of Ts from lymph node T cells obtained from antigen-primed rats indicated that Ts were induced in the presence of the priming antigen within 48 hr of culturing. The Ts produced during the first 48 hr of in vitro cultures were radiosensitive (2000 rad) but became partially radioresistant within the next 48 hr of culturing. In the presence but not the absence of priming antigen, Ts inhibited the antigen-dependent proliferative response to the priming antigen as well as to heterologous antigens. Suppression appeared to be mediated via a nondialyzable suppressor factor (TsF). The induction of Ts in cultures required the presence of OX-6-/OX-8- T cells, antigen-presenting cells, and the antigen. Although a majority of cells recovered from the induced cultures were OX-8+, there was no evidence that OX-8+ antigen expression per se was related to Ts activity. Addition of highly purified IL 2 augmented the Ts-mediated suppression. The immunoregulatory implications of these findings are discussed.