RESUMEN
The antiviral efficacy of phosphonoformic acid (PFA) was examined on tissue cultures of the human embryonal lungs against the viruses herpes simplex type 1 and 2, herpes zoster, and the human cytomegalovirus using the method of the inhibition of the cytopathic effect and against herpes simplex type 1 and 2 on the tissue cultures of Vero cells using the methods of the inhibition of plaque formation. PFA was demonstrated to inhibit the reproduction of the viruses under study in both tests on tissue cultures. In persistence studies, the cytomegalovirus was not isolated back from the cells of the human embryonal lungs, which gave evidence of its greater sensitivity to PFA. In in vivo experiments, PFA in the form of a 3% ointment suppressed herpetic dermatitis on the guinea-pig skin, when treatment started 6, 24 and 48 hours after infection. In a preliminary experiment on rabbit corneas, an ointment with 3% PFA inhibited herpetic keratoconjunctivitis in time intervals of 1 and 3 hours after infection.
Asunto(s)
Antivirales/uso terapéutico , Infecciones por Herpesviridae/tratamiento farmacológico , Ácido Fosfonoacético/análogos & derivados , Animales , Antivirales/farmacología , Foscarnet , Cobayas , Herpesviridae/efectos de los fármacos , Ácido Fosfonoacético/farmacología , Ácido Fosfonoacético/uso terapéutico , ConejosAsunto(s)
Antivirales , Pirazoles , Quinolinas , Quinolinas/uso terapéutico , Animales , Antivirales/uso terapéutico , Virus de la Encefalomiocarditis , Infecciones por Enterovirus/tratamiento farmacológico , Ratones , Infecciones por Orthomyxoviridae/tratamiento farmacológico , Pirazoles/síntesis química , Pirazoles/uso terapéutico , Quinolinas/síntesis químicaRESUMEN
The antiviral effect of 3 derivatives of benzo(c)fluorenone against encephalomyocarditis (EMC) and vaccinia viruses in mice was compared with that of tilorone hydrochloride (THCl). All 3 derivatives were effective against either virus following single-dose oral application as well as after 4-times repeated subcutaneous (s.c.) treatment, but following oral administration only the VUFB 14162 derivative exerted an antiviral effect corresponding to that of THCl. After s.c. application, however, VUFB 14162 derivative was less toxic than THCl. Two benzo(c)fluorenone derivatives, namely VUFB 14162 and 13431, induced in mouse sera the levels of interferon (IFN), which production kinetics and hyporeactivity phenomenon were comparable with those induced by THCl. Because no IFN was found following administration of the third VUFB 13371 derivative, its antiviral effect consisted probably in an other than IFN-inducing mechanism.
Asunto(s)
Antivirales/uso terapéutico , Infecciones por Enterovirus/tratamiento farmacológico , Fluorenos/uso terapéutico , Tilorona/uso terapéutico , Vaccinia/tratamiento farmacológico , Animales , Antivirales/administración & dosificación , Virus de la Encefalomiocarditis , Inductores de Interferón/uso terapéutico , Ratones , Tilorona/administración & dosificación , Tilorona/análogos & derivadosRESUMEN
The retention behaviour of some nitrobenzene derivatives was studied by reversed-phase chromatography on the macroporous polystyrene gel Separon SE with methanol-water, acetonitrile-water, tetrahydrofuran-water and methanol-water with adjusted pH as the eluents. The effect of the mobile phase and the presence of additional polar and non-polar substituents in the molecule of the solute on the retention were investigated. Using methanol-water (80:20) the contribution of the nitro group to the retention of the molecule is positive for substances containing both polar and non-polar functional groups. The contribution of the nitro group did not change if a mobile phase with adjusted pH was used. With acetonitrile-water and tetrahydrofuran-water the situation was more complicated.