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1.
Brain Behav Immun ; 80: 500-511, 2019 08.
Artículo en Inglés | MEDLINE | ID: mdl-31022457

RESUMEN

Overweight and obesity are a worldwide pandemic affecting billions of people. These conditions have been associated with a chronic low-grade inflammatory state that is recognized as a risk factor for a range of somatic diseases as well as neurodevelopmental disorders, anxiety disorders, trauma- and stressor-related disorders, and affective disorders. We previously reported that the ingestion of a high-fat diet (HFD; 45% fat kcal/g) for nine weeks was capable of inducing obesity in rats in association with increased reactivity to stress and increased anxiety-related defensive behavior. In this study, we conducted a nine-week diet protocol to induce obesity in rats, followed by investigation of anxiety-related defensive behavioral responses using the elevated T-maze (ETM), numbers of FOS-immunoreactive cells after exposure of rats to the avoidance or escape task of the ETM, and neuroinflammatory cytokine expression in hypothalamic and amygdaloid nuclei. In addition, we investigated stress-induced cutaneous thermoregulatory responses during exposure to an open-field (OF). Here we demonstrated that nine weeks of HFD intake induced obesity, in association with increased abdominal fat pad weight, increased anxiety-related defensive behavioral responses, and increased proinflammatory cytokines in hypothalamic and amygdaloid nuclei. In addition, HFD exposure altered avoidance- or escape task-induced FOS-immunoreactivity within brain structures involved in control of neuroendocrine, autonomic, and behavioral responses to aversive stimuli, including the basolateral amygdala (BLA) and dorsomedial (DMH), paraventricular (PVN) and ventromedial (VMH) hypothalamic nuclei. Furthermore, rats exposed to HFD, relative to control diet-fed rats, responded with increased tail skin temperature at baseline and throughout exposure to an open-field apparatus. These data are consistent with the hypothesis that HFD induces neuroinflammation, alters excitability of brain nuclei controlling neuroendocrine, autonomic, and behavioral responses to stressful stimuli, and enhances stress reactivity and anxiety-like defensive behavioral responses.


Asunto(s)
Regulación de la Temperatura Corporal/fisiología , Dieta Alta en Grasa/efectos adversos , Neuroinmunomodulación/fisiología , Amígdala del Cerebelo/metabolismo , Animales , Ansiedad/metabolismo , Trastornos de Ansiedad/metabolismo , Corticosterona , Hipotálamo/metabolismo , Masculino , Obesidad , Proteínas Proto-Oncogénicas c-fos/metabolismo , Ratas , Ratas Wistar , Aumento de Peso
2.
Genet Mol Res ; 16(2)2017 May 10.
Artículo en Inglés | MEDLINE | ID: mdl-28510257

RESUMEN

Water deficit is the main reason for instability in the context of soybean culture. The development of strategies for the selection of more tolerant genotypes is necessary. These strategies include the use of polyethylene glycol 6000 solutions (PEG-6000) for conducting the germination test under conditions of water restriction. Thus, the objective of this study was to determine the osmotic potential and the main characteristics that promote the discrimination of soybean genotypes with regard to water stress tolerance during germination and the vigor test. Thirteen soybean cultivars were used. The seeds were allowed to germinate on sheets of germitest paper moistened in solution with PEG-6000, simulating different levels of water availability, which is expressed as osmotic potential (0.0, -0.2, -0.4, and -0.6 MPa). We assessed germination, length, and dry mass for seedlings and seeds, as well as reserve dynamics. Germination and variables related to the dynamics of reservation have great influence on the expression of variability in environments under stress. Among the different osmotic potentials, the -0.2 MPa was the most efficient for the expression of genetic variability among the cultivars. Conducting the germination test with PEG-6000 solution to -0.2 MPa was efficient for selecting soybean cultivars tolerant to water stress. This was accomplished by evaluating the percentage of germination, along with variables related to the dynamics of reservation.


Asunto(s)
Sequías , Genotipo , Germinación/genética , Glycine max/genética , Fitomejoramiento/métodos , Selección Genética , Adaptación Fisiológica , Presión Osmótica , Glycine max/crecimiento & desarrollo , Glycine max/fisiología
3.
Rev Sci Instrum ; 88(12): 125005, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-29289169

RESUMEN

Signal attenuation limits the operating range in wireless communications and location. To solve the reduced range problem, we can use low-frequency signals in combination with magnetic sensing. We propose the use of an optically pumped magnetometer as a sensor and realize a proof-of-principle detection of binary phase shift keying (BPSK) modulated signals. We demonstrate a ranging enhancement by exploiting both the magnetometer's intrinsic sensitivity of below 1 pT/Hz1/2 and its 1 kHz operating bandwidth through the use of BPSK signals.

4.
Rev Sci Instrum ; 79(1): 013709, 2008 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-18248041

RESUMEN

We present the design and fabrication of a magnetoresistive imaging system capable of scanning 256 channels simultaneously along linear ranges of either 4 or 13 mm. High speed electronics read the channels and transfer the data to a computer that builds and displays the images. The system was successfully used to image magnetic tapes for real-time forensic validation purposes and can also be applied in other noninvasive imaging applications.


Asunto(s)
Aumento de la Imagen/instrumentación , Interpretación de Imagen Asistida por Computador/instrumentación , Magnetismo/instrumentación , Procesamiento de Señales Asistido por Computador , Transductores , Grabación en Video/instrumentación , Diseño de Equipo , Análisis de Falla de Equipo , Aumento de la Imagen/métodos , Interpretación de Imagen Asistida por Computador/métodos , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Grabación en Video/métodos
5.
J Res Natl Inst Stand Technol ; 113(1): 1-10, 2008.
Artículo en Inglés | MEDLINE | ID: mdl-27096108

RESUMEN

In this paper we present the development of a magnetic moment reference material for low moment magnetic samples. We first conducted an inter-laboratory comparison to determine the most useful sample dimensions and magnetic properties for common instruments such as vibrating sample magnetometers (VSM), SQUIDs, and alternating gradient field magnetometers. The samples were fabricated and then measured using a vibrating sample magnetometer. Their magnetic moments were calibrated by tracing back to the NIST YIG sphere, SRM 2853.

6.
J Res Natl Inst Stand Technol ; 108(2): 125-34, 2003.
Artículo en Inglés | MEDLINE | ID: mdl-27413599

RESUMEN

A potential low magnetic moment standard reference material (SRM) was studied in an interlaboratory comparison. The mean and the standard deviation of the saturation moment m s, the remanent moment m r, and the intrinsic coercivity H c of nine samples were extracted from hysteresis-loop measurements. Samples were measured by thirteen laboratories using inductive-field loopers, vibrating-sample magnetometers, alternating-gradient force magnetometers, and superconducting quantum-interference-device magnetometers. NiFe films on Si substrates had saturation moment measurements reproduced within 5 % variation among the laboratories. The results show that a good candidate for an SRM must have a highly square hysteresis loop (m r/m s > 90 %), H c ≈ 400 A·m(-1) (5 Oe), and m s ≈ 2 × 10(-7) A·m(2) (2 × 10(-4) emu).

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