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Microencapsulation of probiotic cells is now under special attention because it is considered as the best method for improving the survivability of probiotics. In the present study, alginate, alginate-skim milk and chitosan coated alginate-skim milk coated microspheres of probiotics such as L.rhamnosus and L.plantarum were prepared. The encapsulating efficiency was found to be above 96% for L.plantarum and above 98% for L.rhamnosus. The skim milk was found to be a better matrix for encapsulation and chitosan coating, making the microspheres stronger compared to uncoated microspheres. The stability study of encapsulated and non-encapsulated probiotic was done for a month. The tolerance of encapsulated probiotics in artificial gastric fluid and release profile of probiotic in simulated intestinal fluid was also studied. This is the first study where chitosan coated alginate-skim milk was used for probiotic encapsulation and suggests promising encapsulating/enfolding material for improving the probiotics survivability.

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In this study, a bionanocomposite based food packaging film was designed to reduce environmental hazards. Cellulose nanoparticles (CNP) were prepared from the non-edible part of jackfruit (Artocarpus heterophyllus) by alkaline treatment, bleaching, and acid hydrolysis. The newly designed bionanocomposite film consists of CNP, polyvinyl alcohol (PVA) and fennel seed essential oil (EO). The composition of the film was optimized using central composite design of response surface methodology. Quadratic models were developed for responses such as elongation at break, tensile strength, free radical scavenging (antioxidant) activity, and antimicrobial activity. The optimized film reported seven-fold increase in tensile strength and six-fold in elongation at break compared to PVA film due to incorporation of CNP. Quality of the optimized film was further confirmed by evaluating morphological features, surface characteristics, physical and thermal properties. The results suggest that the newly designed EO incorporated CNP-PVA bionanocomposite film as a better alternative for traditional food packaging materials.

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BACKGROUND AND OBJECTIVES: To determine the immunohistochemical (IHC) localization of basement membrane component laminin in histological grades of oral squamous cell carcinoma (OSCC). The purpose of this study was to demonstrate the loss of continuity of the basement membrane in OSCC using an antibody directed against laminin using advanced polymer staining system. MATERIALS AND METHODS: A total of 30 cases of OSCC: 10 cases of well differentiated squamous cell carcinom (WDSCC), 10 cases of moderately differentiated squamous cell carcinoma (MDSCC), and 10 cases of poorly differentiated squamous cell carcinoma (PDSCC) were subjected to heat-induced antigen retrieval method using ethylene-di-amine-tetraacetic acid buffer in a microwave oven. Then the sections were stained with anti-laminin polyclonal antibody and visualized using super sensitive polymer horseradish peroxidase detection system. In each case, the integrity of the basement membrane laminin was assessed by using statistical analysis. RESULTS: Statistical analysis showed a decreased distribution of laminin from WDSCC to MDSCC to PDSCC (P value 0.0573). The intracytoplasmic staining of laminin gradually increased from WDSCC to MDSCC to PDSCC (P value 0.0198). INTERPRETATION AND CONCLUSION: WDSCC cases showed more laminin expression in basement membrane around the tumor islands and less loss of continuity compared to MDSCC and PDSCC cases suggesting a greater enzymatic degradation of basement membrane components in MDSCC and PDSCC than WDSCC. The loss of structural basement membrane laminin and the presence of laminin in the tumor cells of PDSCC cases suggest that laminin helps in tumor invasion. The expression of laminin in the basement membrane may be a useful parameter to evaluate tumor histologic differentiation and aggressiveness.

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Ameloblastoma is a benign locally invasive epithelial odontogenic tumour comprising 1% of all tumours and cysts arising in the jaws. It is commonly found in the third and fourth decade in the molar ramus region of the mandible. Among all types of ameloblastoma, multicystic ameloblastoma is believed to be locally aggressive lesion that has the tendency for recurrence. In this report we present a large multicystic ameloblastoma in the left body-ramus region of the mandible in a 55-year-old woman. This large lesion was diagnosed with the help of CT and was successfully managed by hemimandibulectomy with simultaneous reconstruction using iliac crest bone.

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