RESUMEN
Musk melon is the favorite fruit with a high market value in Japan, and the fragrance is one of the major factors determining the fruit quality of melon. In this study, mutant melon hairy roots which had been induced by means of the T-DNA insertion mutagenesis were found to produce volatile compounds with the fruity fragrance of mature melon. The volatile compounds were extracted and identified by GLC-mass spectrometry. Some essential oils such as (Z)-3-hexenol, (E)-2-hexenal, 1-nonanol, and (Z)-6-nonenol were stably synthesized by these hairy roots despite the increased number of subcultures. The productivity of these compounds by the best hairy root line was shown to be considerably higher than naturally ripened melon fruits.
Asunto(s)
Cucurbitaceae/fisiología , Odorantes/análisis , Aceites Volátiles/análisis , Raíces de Plantas/fisiología , Cucurbitaceae/genética , ADN Bacteriano , ADN de Cadena Simple , Cromatografía de Gases y Espectrometría de Masas , Mutagénesis Insercional , Aceites Volátiles/metabolismoRESUMEN
New polyisoprenepolyols (hypsiziprenol AA and BA) were isolated from an edible mushroom (Hypsizigus marmoreus). These polyols occur as a mixture of homologous polyisoprene derivatives with 40-70 carbon atoms. Analyses by FAB/MS in the positive and negative ion modes are complementary with each other in that the former provides information on the number of hydroxy groups present while the latter specifies the isoprenoid sequence, and thus become a powerful tool for analyzing the structures of polyisoprenepolyols. No polyisoprenepolyols obtained here were found to have antitumor activity on NCI-H292 and EL-4 cell lines.
Asunto(s)
Agaricales/química , Alcoholes Grasos/química , Terpenos/química , Antineoplásicos/química , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Alcoholes Grasos/farmacología , Humanos , Indicadores y Reactivos , Espectrometría de Masa Bombardeada por Átomos Veloces , Gusto , Terpenos/farmacología , Células Tumorales CultivadasRESUMEN
Three limonoid glycosides were isolated from Citrus unshiu peels, and their structures were determined based on MS and NMR spectroscopic data as nomilinic acid 17-O-beta-D-glucopyranoside (1), methyl nomilinate 17-O-beta-D-glucopyranoside (2), and obacunone 17-O-beta-D-glucopyranoside (3). In particular, the location of the sugar moiety was clearly determined by the B/E constant linked scan FABMS method. No limonoid glycosides obtained here were found to have antitumor activity in NCI-H292 and EL-4 cell lines.
Asunto(s)
Citrus/química , Glicósidos/química , Glicósidos/aislamiento & purificación , Animales , Secuencia de Carbohidratos , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Espectroscopía de Resonancia Magnética , Ratones , Datos de Secuencia Molecular , Células Tumorales CultivadasRESUMEN
Fast atom bombardment (FAB) mass spectrometry is a powerful tool for analyzing the structure of polyisoprenepolyols. Analyses in the positive and negative ion mode are complementary in that the former provides data on the number of hydroxy groups present while the latter provides data on the isoprenoid sequence. Some of this information is already available from routine FAB mass spectra. More detailed information is contained in the linked scan spectra.
Asunto(s)
Basidiomycota/química , Polímeros/química , Espectroscopía de Resonancia Magnética , Polímeros/aislamiento & purificación , Espectrometría de Masa Bombardeada por Átomos VelocesRESUMEN
Four cyclic peptides were isolated from young unshiu (unripe fruit), orange and amanatsu peelings, and their structures were established on the basis of FAB-MS (CID method) and 2D-NMR spectroscopic data, and by chemical evidence. They were each found to consist of seven or eight amino acids.