RESUMEN
This study aims to prove the value of the polyoxazolines polymer family as surfactant in formulations for topical application and as an alternative to PEG overuse. The amphiphilic polyoxazolines (POx) were demonstrated to have less impact on cell viability of mice fibroblasts (NIH3T3) than their PEG counterparts. Mixed micelles, made of POx and phosphatidylcholine, were manufactured using thin film and high pressure homogenizer process. The mixed micelles were optimized to produce nanosized vesicles of about 20â¯nm with a spherical shape and stable over 28â¯days. The natural lipophilic antioxidant, quercetin, was successfully encapsulated (encapsulation efficiency 94⯱â¯4% and drug loading 3.6⯱â¯0.2%) in the mixed micelles with no morphological variation. Once loaded in the formulation, the quercetin impact on cell viability of NIH3T3 was decreased while its antioxidant activity remained unchanged. This work highlights the capacity of amphiphilic POx to create, in association with phospholipids, stable nanoformulations which show promise for topical delivery of antioxidant and ensure skin protection against oxidative stress.
Asunto(s)
Antioxidantes/administración & dosificación , Antioxidantes/química , Oxazolona/análogos & derivados , Polietilenglicoles/química , Polímeros/química , Quercetina/administración & dosificación , Quercetina/química , Administración Tópica , Animales , Línea Celular , Supervivencia Celular/efectos de los fármacos , Química Farmacéutica/métodos , Portadores de Fármacos/química , Sistemas de Liberación de Medicamentos/métodos , Liberación de Fármacos/efectos de los fármacos , Fibroblastos/efectos de los fármacos , Ratones , Micelas , Células 3T3 NIH , Oxazolona/química , Estrés Oxidativo/efectos de los fármacos , Tamaño de la PartículaRESUMEN
Lung cancer is a frequent pathology among women, as a result of tobacco increase. Lung cancer among pregnant women is especially rare when revealed by Pancoast-Tobias syndrome. Foetal injury is possible. The approach is multidisciplinary. Prognosis is bad.
Asunto(s)
Neoplasias Pulmonares/complicaciones , Síndrome de Pancoast/complicaciones , Complicaciones Neoplásicas del Embarazo/etiología , Resultado del Embarazo , Adulto , Cesárea , Resultado Fatal , Femenino , Humanos , Recién Nacido , Neoplasias Pulmonares/patología , Síndrome de Pancoast/patología , Embarazo , Complicaciones Neoplásicas del Embarazo/patología , Pronóstico , Factores de RiesgoRESUMEN
A complete diet was prepared with cooked pieces of meat, beans, cream starch, and water and presented to the rats in two different textures: a blended purée and a rough mixture that required a lot of chewing. We hypothesized that this texture modification might change both anticipatory reflexes and feeding behavior. Feeding rate, meal size, intermeal intervals, and their correlation were monitored in response to each texture. The long-term (6 wk) effect on body weight was assessed. Periprandial plasma glucose, insulin, glucagon, and lipid concentrations were assayed. Whole and background metabolism, respiratory quotient, and locomotion were measured using a computerized calorimeter of original design. In the short term, rats preferred the mixture. However, after 3 wk, they ingested more purée than mixture and gained more body weight per gram of food ingested as purée. Insulin response declined earlier with the mixture. During meals, glycerol and free fatty acid increased earlier with purée, whereas in the postprandial period, glycerol increased earlier with mixture. The metabolic rate, however, was not significantly affected. We concluded that texture, an everyday manipulation performed on food for human consumption, affects not only palatability of ingestants but also their metabolic management in the short and long term.