RESUMEN

In Trial 1, rams (n=26) were fed different levels (0, 250,000, 500,000 or 750,000 IU) of vitamin D(3) for 4 days to determine the most effective dose to increase blood calcium concentrations. Trial 2 consisted of feeding feedlot lambs (n=40) different levels (0 or 750,000 IU) of vitamin D(3) for 14 days to determine if vitamin D(3) could improve the tenderness of lamb muscles. Lambs were slaughtered and the M. longissimus lumborum, M. biceps femoris, M. semitendinosus, and M. semimembranosus were removed after chilling, cut into chops, and assigned to an aging period (5, 10 or 15 days) for Warner-Bratzler shear force (WBS) determination. In Trial 1, feed intake and weight gain were lower for rams supplemented with 500,000 IU of vitamin D(3) compared to all other groups. Blood calcium concentrations were not different between groups, although the 750,000 IU group tended (P<0.10) to have higher blood calcium concentrations on day 5 of the trial compared to controls. In Trial 2, blood calcium concentrations were not different between the treated and control groups, however, treated lambs had higher (P<0.01) calcium concentrations in both the liver and kidneys. Control chops from the M. longissimus lumborum had lower (P<0.05) WBS values than chops from vitamin D(3) fed lambs, but no other muscles were affected by vitamin D(3) feeding. An interaction between treatment and aging was observed for the M. biceps femoris, with chops from vitamin D(3) fed lambs having lower WBS values at 5 days aging, but chops from control lambs having lower WBS values at 15 days aging. WBS values decreased for the M. longissimus lumborum, M. semitendinosus, and M. semimembranosus with increasing aging time. Vitamin D(3) supplementation was not an effective means of improving the tenderness characteristics of lamb muscles.

RESUMEN

Endometrial glands secrete molecules hypothesized to support conceptus growth and development. In sheep, endometrial gland morphogenesis occurs postnatally and can be epigenetically ablated by neonatal progestin exposure. The resulting stable adult uterine gland knockout (UGKO) phenotype was used here to test the hypothesis that endometrial glands are required for successful pregnancy. Mature UGKO ewes were bred repeatedly to fertile rams, but no pregnancies were detected by ultrasound on Day 25. Day 7 blastocysts from normal superovulated ewes were then transferred synchronously into Day 7 control or UGKO ewes. Ultrasonography on Days 25-65 postmating indicated that pregnancy was established in control, but not in UGKO ewes. To examine early uterine-embryo interactions, four control and eight UGKO ewes were bred to fertile rams. On Day 14, their uteri were flushed. The uterus of each control ewe contained two filamentous conceptuses of normal length. Uteri from four UGKO ewes contained no conceptus. Uteri of three UGKO ewes contained a single severely growth-retarded tubular conceptus, whereas the remaining ewe contained a single filamentous conceptus. Histological analyses of these uteri revealed that endometrial gland density was directly related to conceptus survival and developmental state. Day 14 UGKO uteri that were devoid of endometrial glands did not support normal conceptus development and contained either no conceptuses or growth-retarded tubular conceptuses. The Day 14 UGKO uterus with moderate gland development contained a filamentous conceptus. Collectively, these results demonstrate that endometrial glands and, by inference, their secretions are required for periimplantation conceptus survival and development.

Asunto(s)

Desarrollo Embrionario , Desarrollo Embrionario y Fetal , Endometrio/fisiología , Ovinos/embriología , Animales , Blastocisto/fisiología , Dinoprost/administración & dosificación , Dinoprost/farmacología , Implantes de Medicamentos , Transferencia de Embrión , Endometrio/anatomía & histología , Endometrio/efectos de los fármacos , Femenino , Interferón Tipo I/análisis , Embarazo , Proteínas Gestacionales/análisis , Pregnenodionas/administración & dosificación , Pregnenodionas/farmacología , Progestinas/administración & dosificación , Progestinas/farmacología , Superovulación , Ultrasonografía Prenatal

RESUMEN

Ovine endometrial gland development is a postnatal event that can be inhibited epigenetically by chronic exposure of ewe lambs to a synthetic progestin from birth to puberty. As adults, these neonatally progestin-treated ewes lack endometrial glands and display a uterine gland knockout (UGKO) phenotype that is useful as a model for study of endometrial function. Here, objectives were to determine: 1) length of progestin exposure necessary from birth to produce the UGKO phenotype in ewes; 2) if UGKO ewes display normal estrous cycles; and 3) if UGKO ewes could establish and/or maintain pregnancy. Ewe lambs (n = 22) received a Norgestomet (Nor) implant at birth and every two weeks thereafter for 8 (Group I), 16 (Group II), or 32 (Groups III and IV) weeks. Control ewe lambs (n = 13) received no Nor treatment (Groups V and VI). Ewes in Groups I, II, III, and VI were hemihysterectomized (Hhx) at 16 weeks of age. After puberty, the remaining uterine horn in Hhx ewes was removed on either Day 9 or 15 of the estrous cycle (Day 0 = estrus). Histological analyses of uteri indicated that progestin exposure for 8, 16, or 32 weeks prevented endometrial adenogenesis and produced the UGKO phenotype in adult ewes. Three endometrial phenotypes were consistently observed in Nor-treated ewes: 1) no glands, 2) slight glandular invaginations into the stroma, and 3) limited numbers of cyst- or gland-like structures in the stroma. Overall patterns of uterine progesterone, estrogen, and oxytocin receptor expression were not different in uteri from adult cyclic control and UGKO ewes. However, receptor expression was variegated in the ruffled luminal epithelium of uteri from UGKO ewes. Intact UGKO ewes displayed altered estrous cycles with interestrous intervals of 17 to 43 days, and they responded to exogenous prostaglandin F(2 approximately ) (PGF) with luteolysis and behavioral estrus. During the estrous cycle, plasma concentrations of progesterone in intact control and UGKO ewes were not different during metestrus and diestrus, but levels did not decline in many UGKO ewes during late diestrus. Peak peripheral plasma concentrations of PGF metabolite, in response to an oxytocin challenge on Day 15, were threefold lower in UGKO compared to control ewes. Intact UGKO ewes bred repeatedly to intact rams did not display evidence of pregnancy based on results of ultrasound. Collectively, results indicate that 1) transient, progestin-induced disruption of ovine uterine development from birth alters both structural and functional integrity of the adult endometrium; 2) normal adult endometrial integrity, including uterine glands, is required to insure a luteolytic pattern of PGF production; and 3) the UGKO phenotype, characterized by the absence of endometrial glands and a compact, disorganized endometrial stroma, limits or inhibits the capacity of uterine tissues to support the establishment and/or maintenance of pregnancy.

Asunto(s)

Estro/fisiología , Útero/metabolismo , Animales , Dinoprost/análogos & derivados , Dinoprost/sangre , Dinoprost/metabolismo , Femenino , Hibridación in Situ , Oxitocina/farmacología , Fenotipo , Embarazo , Pregnenodionas/farmacología , Progesterona/sangre , Progesterona/metabolismo , Radioinmunoensayo , Receptores de Estrógenos/biosíntesis , Receptores de Estrógenos/efectos de los fármacos , Receptores de Progesterona/biosíntesis , Receptores de Progesterona/efectos de los fármacos , Ovinos

RESUMEN

The response to natural infection by gastrointestinal nematodes was evaluated in three groups of lambing mature ewes: 16 Florida Native; 21 Rambouillet; and 18 1/2 Florida Native x1/2 Rambouillet (F1 generation). Fecal egg counts (FEC), packed cell volume (PCV) and plasma protein levels were determined periodically through a one-year period. The highest FEC was recorded in Rambouillet ewes, with most of the means higher than 1000 eggs per gram (EPG). Florida Native and F1 ewes showed similar trends in FEC, with most of the means below 500 EPG. The highest mean PCV values were recorded in Florida Native ewes, followed in decreasing order by F1 and Rambouillet ewes. F1 ewes showed the lowest plasma protein levels and Florida Natives the highest. Fecal cultures revealed the highest percentages of Haemonchus larvae in Rambouillet ewes (>64%) and the lowest percentages in Florida Native ewes. Florida Native and F1 ewes were more resistant to gastrointestinal nematode infections than were Rambouillet ewes. High negative correlation coefficients between FEC x PCV and FEC x plasma protein levels coincided with peaks in mean FEC of Rambouillet (May and July), Florida Native and F1 ewes (October). This study showed that crossbreeding Rambouillet and Florida Native sheep resulted in the production of F1 ewes resistant against gastrointestinal nematode infections.

Asunto(s)

Hemoncosis/veterinaria , Haemonchus/crecimiento & desarrollo , Parasitosis Intestinales/veterinaria , Enfermedades de las Ovejas/parasitología , Animales , Antihelmínticos/farmacología , Antihelmínticos/uso terapéutico , Proteínas Sanguíneas/análisis , Cruzamientos Genéticos , Resistencia a Medicamentos , Heces/parasitología , Femenino , Hemoncosis/genética , Hemoncosis/parasitología , Haemonchus/efectos de los fármacos , Hematócrito/veterinaria , Parasitosis Intestinales/genética , Parasitosis Intestinales/parasitología , Masculino , Recuento de Huevos de Parásitos/veterinaria , Embarazo , Ovinos , Enfermedades de las Ovejas/genética

RESUMEN

Interferon tau (IFNtau) is the antiluteolytic signal produced by the conceptus of ruminants. Intrauterine administration of recombinant ovine IFNtau suppresses expression of endometrial estrogen receptor (ER) and oxytocin receptor (OTR) in the luminal and superficial glandular epithelia to abrogate the production of luteolytic prostaglandin F(2alpha) (PGF(2alpha)) pulses. Subcutaneous (s.c.) injections of recombinant ovine (o) IFNtau appear to extend the interestrous interval by altering uterine PGF(2alpha) response to oxytocin. The present study tested the hypothesis that antiluteolytic effects of roIFNtau injected into the uterine lumen (paracrine) or s.c. (endocrine) are equivalent in suppressing expression of endometrial ER and OTR and inducing uterine expression of type I IFN-regulated Mx and ubiquitin cross-reactive proteins (UCRP). Sixteen cyclic ewes were fitted with uterine catheters on Day 5 (Day 0 = estrus), were assigned randomly to receive treatment with control proteins or roIFNtau (2 x 10(7) antiviral units/day) by either intrauterine or s.c. injections from Days 11 to 15, and were ovariohysterectomized on Day 16. Results indicated that expression of ER and OTR mRNAs in endometrial epithelium was suppressed by intrauterine but not by s.c. injections of roIFNtau. Intrauterine injections of roIFNtau increased expression of Mx and UCRP mRNA in the endometrium. Subcutaneous injections of roIFNtau increased endometrial Mx mRNA levels but not UCRP mRNA. Unexpectedly, intrauterine and s.c. injections of roIFNtau were equally effective in inducing expression of Mx and UCRP mRNA in the corpus luteum. Although s.c. injections of roIFNtau induced Mx mRNA in the endometrial epithelium, s.c. injections of roIFNtau did not abrogate activation of the uterine luteolytic mechanism by suppressing epithelial ER and OTR expression. Therefore, results of this study failed to support the assumption that endocrine roIFNtau mimics antiluteolytic effects of paracrine IFNtau to improve pregnancy rates in sheep.

Asunto(s)

Endometrio/efectos de los fármacos , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Interferón Tipo I/administración & dosificación , Interferón Tipo I/farmacología , Proteínas Gestacionales/administración & dosificación , Proteínas Gestacionales/farmacología , Animales , Antivirales/biosíntesis , Antivirales/genética , Proteínas Portadoras/biosíntesis , Proteínas Portadoras/genética , Femenino , GTP Fosfohidrolasas/biosíntesis , GTP Fosfohidrolasas/genética , Proteínas de Unión al GTP/biosíntesis , Proteínas de Unión al GTP/genética , Hibridación in Situ , Inyecciones Subcutáneas , Leucina Zippers , Proteínas de Resistencia a Mixovirus , Embarazo , Biosíntesis de Proteínas , Proteínas/genética , Receptores de Estrógenos/biosíntesis , Receptores de Estrógenos/genética , Receptores de Oxitocina/biosíntesis , Receptores de Oxitocina/genética , Proteínas Recombinantes/administración & dosificación , Proteínas Recombinantes/farmacología , Ovinos , Ubiquitinas/análogos & derivados , Ubiquitinas/biosíntesis , Ubiquitinas/genética , Útero

RESUMEN

The Cu status of mature, crossbred ewes fed two sources (CuSO4 vs. Cu proteinate) and three levels (10, 20, or 30 mg/kg) of dietary Cu was determined in a 73-d feeding trial. Ewes (n = 30) were fed a basal diet containing rice meal feed, cottonseed hulls, cottonseed meal, meat and bone meal, cracked corn, and vitamin-mineral supplements at 2.5% of BW to meet NRC requirements for protein, energy, macrominerals, and microminerals, excluding Cu. The basal diet contained 5 mg/kg Cu, 113 mg/kg Fe, .1 mg/kg Mo, and .17% S. Copper sulfate or Cu proteinate was added to the basal diet to supply 10, 20, or 30 mg/kg of dietary copper in a 2x3 factorial arrangement of treatments. Ewes were housed in 3.7- x 9.1-m pens in an open-sided barn. Blood samples were collected on d 28 and 73. Ewes were slaughtered on d 74, and liver and other tissues were collected to determine Cu concentrations. An interaction (P = .08) occurred between source and level for liver Cu. The interaction existed due to an increase in liver Cu concentrations when ewes were fed increasing dietary Cu from CuSO4 but not when fed Cu proteinate diets. There was no source x level interaction (P>.10) for the blood constituents measured. On d 73, plasma ceruloplasmin activity was greater (P<.05) in ewes fed Cu proteinate than in those fed CuSO4 (33.1 vs. 26.8 microM x min(-1) x L(-1)). Increasing the concentration of dietary Cu did not affect (P>.10) plasma ceruloplasmin. Packed cell volume (PCV), red blood cell count (RBC), white blood cell count, whole blood hemoglobin (wHb), plasma hemoglobin, and plasma Cu were similar between sources of Cu. Ewes fed 20 mg/kg Cu had lower (P<.05) PCV, RBC, and wHb than those fed 10 or 30 mg/kg Cu diets. Feeding up to 30 mg/kg Cu from these sources did not cause an observable Cu toxicity during the 73-d period.

Asunto(s)

Alimentación Animal , Sulfato de Cobre/administración & dosificación , Cobre/administración & dosificación , Estado Nutricional , Proteínas/administración & dosificación , Ovinos/metabolismo , Animales , Bilis/química , Cobre/análisis , Cobre/sangre , Cobre/farmacocinética , Sulfato de Cobre/farmacocinética , Proteínas/farmacocinética , Lana/química

RESUMEN

This experiment was carried out to compare the worm burden and cellular responses in the abomasal mucosa and blood of Florida Native and Rambouillet lambs and also in animals produced by crosses of these two breeds (generations F1 and F2). Animals were exposed to infection by gastrointestinal nematodes on three different occasions. The first infection was natural, occurring while they were suckling lambs. After weaning, they were kept indoors for 53 days and then were allowed to graze a contaminated pasture for 50 days for a second natural infection. The third infection was an artificial challenge with 6000 Haemonchus contortus infective larvae. The highest mean fecal egg counts (FEC) values were found in Rambouillet lambs followed in decreasing order by F1, F2 and Florida Native lambs. Throughout the trial, most of the high mean packed cell volumes and plasma protein levels were recorded in the F2 lambs; in contrast, most of the low values were found in the Rambouillet lambs. During the natural infection period, the highest percentages of larvae in the fecal cultures of the lambs were Haemonchus. However, high percentages of Trichostrongylus larvae were found particularly in Florida Native lambs. The mean number of blood eosinophils increased after the artificial challenge, reached a peak 21 days after infection and then declined. The highest and lowest blood eosinophil means were recorded in F2 and Florida Native lambs, respectively. The H. contortus burden was significantly higher in Rambouillet and in F1 lambs than in Florida Native and F2 lambs (p < 0.05), while no significant differences were recorded among eosinophil, mast cell and globule leucocyte counts in the abomasal mucosa (p > 0.05). The highest correlation coefficient recorded at the end of this study was between FEC and worm burden (r = 0.7). These two parameters showed a moderate negative correlation with PCV, plasma protein and mast cell counts in the abomasal mucosa. The results obtained in this study indicate that crossbreeding Florida Native and Rambouillet sheep can be a rapid way to combine and improve the characteristics of these two breeds. The parasitological results were promising, however, more studies will be necessary to verify the impact of crossbreeding in other traits.

Asunto(s)

Hemoncosis/veterinaria , Haemonchus/inmunología , Enfermedades de las Ovejas/inmunología , Abomaso/inmunología , Abomaso/parasitología , Animales , Animales Lactantes , Proteínas Sanguíneas/análisis , Cruzamiento , Cruzamientos Genéticos , Eosinófilos/citología , Heces/parasitología , Femenino , Hemoncosis/genética , Hemoncosis/inmunología , Hemoncosis/prevención & control , Hematócrito/veterinaria , Inmunidad Innata , Recuento de Leucocitos/veterinaria , Masculino , Mastocitos/citología , Recuento de Huevos de Parásitos/veterinaria , Refractometría/veterinaria , Ovinos , Enfermedades de las Ovejas/genética , Enfermedades de las Ovejas/prevención & control

RESUMEN

Suffolk and Targhee ewes (30 each) with single or twin lambs were used in four periods beginning in late gestation and continuing through weaning to evaluate breed differences in milk production, lamb BW, and DMI by ewes and lambs. In Periods 1 (late gestation) and 2 (early lactation), ewes (Period 1) and ewes with lambs (Period 2) were individually penned, fed .45 kg of barley x ewe(-1) x d(-1) and allowed ad libitum access to chopped alfalfa. Ewes and lambs grazed native range in Periods 3 and 4. Grazed forage DMI was estimated using chromic oxide. Estimates of milk production were obtained by handmilking. Average lamb age was 4, 45, and 73 d at the beginning of Periods 2, 3, and 4, respectively. Milk production tended (P = .20) to be greater for Suffolk than for Targhee ewes. Targhee ewes produced 85% more (P = .001) wool than Suffolk ewes. From 33 d prepartum to 89 d postpartum, Suffolk ewes consistently weighed more (P = .001) than Targhee ewes. Suffolk ewe BW loss (-.15 kg/d) was greater (P = .01) than Targhee ewe BW loss (-.02 kg/d) from 33 d prepartum to 6 d postpartum. From 6 to 89 d postpartum BW gain did not differ (P = .69; .05 kg/d) between breeds. From birth to 89 d postpartum, Suffolk lambs consistently weighed more than Targhee lambs (P = .003). From birth to 89 d postpartum, ADG was greater for Suffolk than for Targhee lambs (P = .006). Targhee ewes consumed 25% more (P = .01) feed over the course of the study than did Suffolk ewes. Grazed forage DMI by Targhee lambs was 26% greater (P = .01) than DMI by Suffolk lambs. When meat production is the primary income from sheep, one potential advantage of Suffolks compared with Targhees is more rapid gain with less feed intake.

Asunto(s)

Animales Lactantes/fisiología , Ingestión de Alimentos/fisiología , Lactancia/fisiología , Ovinos/fisiología , Alimentación Animal , Animales , Peso Corporal , Cruzamiento , Dieta/normas , Dieta/veterinaria , Femenino , Leche/química , Poaceae , Periodo Posparto/fisiología , Gemelos , Lana/crecimiento & desarrollo

RESUMEN

Targhee ewes with single or twin lambs were used in four trials during early lactation through weaning to evaluate ewe milk production (MP), kilograms of lamb weaned, and forage intake by ewes and lambs. During Trial 1, ewes with lambs were individually penned, fed .45 kg of barley-ewe-1.d-1 and allowed ad libitum access to chopped alfalfa hay. Trials 2, 3, and 4 were conducted on three different types of Western range environments. Forage intake of ewes in Trials 2, 3, and 4 and of lambs in Trials 3 and 4 was estimated using chromic oxide. Estimates of milk production were obtained by hand-milking. Average lamb age was 4, 50, 78, and 106 d at the beginning of Trials 1, 2, 3, and 4, respectively. Milk production was greater (P = .01) for ewes nursing twins than for those nursing singles at d 4 and 14 in Trial 1. In Trials 2, 3, and 4, all ewes had similar (P > .18) milk production. Ewe DMI during Trials 1 and 2 was greater (P < .05) for ewes nursing twin lambs than for those nursing singles. There was no difference (P > .88) in DMI between ewes with single and those with twin lambs in Trials 3 and 4. Forage DMI by twin lambs, expressed as either kilograms.ewe-1.day-1 or percentage of lamb BW, was higher (P = .01) than intake by single lambs in Trials 3 and 4.(ABSTRACT TRUNCATED AT 250 WORDS)

Asunto(s)

Animales Lactantes/fisiología , Ingestión de Alimentos , Lactancia/fisiología , Ovinos/fisiología , Alimentación Animal , Crianza de Animales Domésticos , Animales , Animales Lactantes/crecimiento & desarrollo , Cruzamiento , Femenino , Poaceae , Distribución Aleatoria , Análisis de Regresión , Ovinos/crecimiento & desarrollo , Gemelos , Aumento de Peso

RESUMEN

Twelve ruminally cannulated wethers (four with duodenal cannulas) were used in a replicated 4 x 4 Latin square to investigate effects of barley variety and restricted vs ad libitum intake on site, rate, and extent of digestion. Diets were based on Ottus (52.7% starch; O) or Steptoe (56.7% starch; S) barley fed at either 90% of prestudy ad libitum feed intake (R) or ad libitum consumption (A). Diets were formulated to be equal in starch content (38%). Periods consisted of 10 d for adaptation followed by 10 d in which fecal, duodenal, ruminal, and in situ collections were made. With the exception of ruminal starch digestion, barley variety did not interact with level of feed intake (P > .26). Intake of OM was 1,009, 996, 1,124, and 881 g.wether-1.d-1 for O, S, A, and R, respectively. Intake by R wethers of OM and starch was 78% (P < .01) of intake by A wethers. Duodenal flow of OM and ruminal pH were not affected (P > .28) by either intake restriction or barley variety. Although restricted intake did not affect ruminal (P = .84) and duodenal (P = .39) ammonia nitrogen, the O barley had higher (P = .01) ruminal and duodenal ammonia nitrogen than did S barley. Restricted intake had no effect (P > .30) on rate of ruminal barley digestion, ruminal and total tract starch digestion, or total tract OM digestion. This may be due to the rapid digestion rate of barley.(ABSTRACT TRUNCATED AT 250 WORDS)

Asunto(s)

Alimentación Animal , Digestión , Ingestión de Alimentos , Hordeum , Ovinos/fisiología , Animales , Duodeno/fisiología , Concentración de Iones de Hidrógeno , Masculino , Rumen/química , Rumen/fisiología , Almidón/metabolismo , Factores de Tiempo

RESUMEN

A centrifugal method has been evaluated for measuring the strength of Vero Green Monkey kidney cell adhesion to growth surfaces. The centrifugal force necessary to remove cells gave a quantitative measure of cell adhesion and hence the quality of the growth surface. After being subjected to high gravity forces, both the remaining attached cells and the detached cells were viable, indicating the detachment process did not simply rupture the cell. Electron microscope examination of growth surfaces after cell detachment suggested that remnants related to filopodia remained.

Asunto(s)

Adhesión Celular , Animales , Línea Celular , Centrifugación/métodos , Chlorocebus aethiops , Riñón , Microscopía Electrónica de Rastreo , Factores de Tiempo

RESUMEN

Photoelectron spectroscopic examination of treated plastic surfaces showed that surface oxidation, primarily as carboxyl groups, was responsible for formation of good growth surfaces. Gas-plasma studies indicated that only very short exposures were required and that the effect was confined to a thin surface layer that produced adhesive surfaces. Highly adhesive surfaces were produced using oxidizing chemicals. Studies with a polymeric ester demonstrated the importance of unesterified carboxyl groups for high adhesiveness.

Asunto(s)

Adhesión Celular , Animales , Ácidos Carboxílicos , Línea Celular , Chlorocebus aethiops , Riñón , Oxígeno , Plásticos

RESUMEN

The feasibility of using columnar reactors containing immobilized microorganisms for the rapid estimation of BOD was demonstrated in this study. Dilutions of three types of industrial effluents were tested by the BOD5 test and by this experimental system. A high degree of correlation (r = 0.98) was observed between results of the two tests. The mean standard error of estimation of the experimental system was 11%.

RESUMEN

Media containing the fluorogenic compound 8-anilino-1-naphthalene sulfonic acid may be used to discriminate between gram-positive and gram-negative bacteria and to differentiate between various species of bacteria. Fluorescent light emitted from colonies of gram-negative bacteria on 8-anilino-1-naphthalene sulfonic acid-containing agar was visually more intense than that on gram-positive bacteria. The emitted light from the gram-negative bacteria differed in wave-lengths from that of light emitted by colonies of gram-positive bacteria. The fluorescent intensity of colonies on complete 8-anilino-1-napthalene sulfonic acid agar supplemented with 1% of single substrates varied depending on the bacterial species, thus allowing the development of profiles used to identify 12 different species.

Asunto(s)

Naftalenosulfonatos de Anilina/farmacología , Bacterias/clasificación , Fluorescencia , Técnicas Bacteriológicas , Pared Celular , Medios de Cultivo , Escherichia coli/efectos de los fármacos , Especificidad de la Especie , Staphylococcus aureus/efectos de los fármacos

RESUMEN

A survey of 26 fluorophores revealed 5 which were non-inhibitory to Staphylococcus aureus and Escherichia coli and produced low background and high colony fluorescence.

Asunto(s)

Colorantes/farmacología , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Naftalenosulfonatos/farmacología , Staphylococcus aureus/crecimiento & desarrollo , Agar , Medios de Cultivo , Staphylococcus aureus/efectos de los fármacos , Relación Estructura-Actividad

Asunto(s)

Manejo de Especímenes/métodos , Anaerobiosis , Clostridium/efectos de los fármacos , Aceites , Siliconas/farmacología

Asunto(s)

Movimiento Celular , Quimiotaxis de Leucocito , Terminología como Asunto , Leucocitos/fisiología

Asunto(s)

Movimiento Celular , Quimiotaxis de Leucocito , Leucocitos/fisiología , Terminología como Asunto

Asunto(s)

Quimiotaxis , Leucocitos/fisiología , Receptores de Droga , Adhesión Celular , Membrana Celular , AMP Cíclico/metabolismo , Humanos , Modelos Biológicos , Neutrófilos/metabolismo

Asunto(s)

Movimiento Celular , Quimiotaxis , Leucocitos/fisiología , Movimiento Celular/efectos de los fármacos , Colchicina/farmacología , Citoplasma/fisiología , Humanos , Técnicas In Vitro , Leucocitos/citología , Películas Cinematográficas , Fotomicrografía