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Objective To investigate the clinical feasibility and effectiveness of 3-D printing (3DP) combined with 3-D laparoscopic nephron-sparing surgery (LNSS) for partial endogenous renal cell carcinoma.Methods A retrospective analysis was made of the clinical data of 79 patients with partial endogenous renal cell carcinoma who were admitted to our department from July 2015 to October 2018.There were 46 males and 33 females.Their average age was (50.9 ± 7.9) years old,ranged from 33 to 68 years old.Tumor stages were T1aN0M0 in 53 cases and T1bN0M0 in 26 cases.The preoperative serum creatinine ranged from 40 to 107 μmol/L,with an average of (72.4 ± 14.2) μmol/L.The preoperative GFR ranged from 19 to 54 ml/min,with an average of (40.2 ± 6.2) ml/min.Thirty-four patients underwent 2-D laparoscopic nephron-sparing surgery (2DLNSS) based on preoperative enhanced CT scans.Forty-five patients underwent 3-D printing (3DP) based on three-dimensional reconstruction of renal CT scans.Seventeen patients underwent 2-D laparoscopic nephron-sparing surgery guided by 3-D printing model(3DP-2DLNSS),and 28 patients underwent 3-D laparoscopic nephron-sparing surgery guided by 3-D printing (3DP-3DLNSS).Serum creatinine levels ranged from 42 to 122 μmol/L with an average of (86.3 ± 14.8) μmol/L,and creatinine levels ranged from 8 to 66 μmol/L with an average of (19.1 ± 14.1) μmol/L.Six months after operation,the GFR of the kidney was 9-36 ml/min with an average of (21.4 ± 6.4)ml/min,and the fluctuation range was 6-40 ml/min with an average of (19.2 ± 8.8) ml/min.There was no statistical difference in the incidence of complications and pathological types after operation.Results There was no statistical difference in general data of preoperative patients.In intraoperative and post-operative statistics,the time of exploring renal artery was shorter than that of 2DLNSS (33.7 ± 7.5) min in 3DP-2DLNSS (28.3 ± 8.2,P =0.015) min and 3DP-3DLNSS (27.8 ± 6.5,P =0.002) min.In tumor detection time,3 DP-2DLNSS was shorter than 2DLNSS group (41.2 ± 6.6 vs.46.5 ± 6.9 min,P =0.012),and 3 DP-3DLNSS was shorter than 3DP-2DLNSS (35.4 ± 7.3 vs.41.2 ± 6.6 min,P =0.009).In warm ischemia time,3DP-2DLNSS min was shorter than 2DLNSS (23.5 ±9.7 vs.33.9 ±7.5 min P ＜0.001),and 3DP-3DLNSS was shorter than 3DP-2DLNSS (18.3 ± 4.6 vs.23.5 ± 9.7,P =0.023).In surgical time,3DP-2DLNSS (115.7 ± 23.0) min and 3DP-3DLNSS (103.3 ± 22.8) min were shorter than 2DLNSS (132.4 ± 28.9) min (P =0.031,P ＜ 0.001).In intraoperative bleeding volume,3 DP-3 DLNSS was less than 2DLNSS (117.9 ± 17.9 vs.130.6 ± 16.8,P =0.009) ml.Fasting for 1 to 4 days after operation,with an average of (1.7 ± 0.8) days.The indwelling catheterization ranged from 1 to 8 days after operation,with an average of (3.9 ± 1.3) days.Negative pressure drainage was removed 2-9 days after operation,with an average of (4.9 ± 1.4) days.And the hospitalization 5-11 days after operation,with an average of (7.3 ± 1.5) days.Conclusions Preoperative 3D printing combined with intraoperative 3D laparoscopic nephron sparing surgery for partial endogenous renal tumors is safe and effective,which is superior to the previous CT scan alone and intraoperative 2D laparoscopic treatment.

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Células Madre Adultas/patología , Transformación Celular Neoplásica/patología , Leiomioma/patología , Leiomiomatosis/patología , Miometrio/patología , Células Madre Neoplásicas/patología , Neoplasias Uterinas/patología , Células Madre Adultas/efectos de los fármacos , Células Madre Adultas/metabolismo , Transformación Celular Neoplásica/efectos de los fármacos , Transformación Celular Neoplásica/metabolismo , Disruptores Endocrinos/toxicidad , Epigénesis Genética/efectos de los fármacos , Femenino , Predisposición Genética a la Enfermedad , Humanos , Leiomioma/inducido químicamente , Leiomioma/genética , Leiomioma/metabolismo , Leiomiomatosis/inducido químicamente , Leiomiomatosis/genética , Leiomiomatosis/metabolismo , Mutación , Miometrio/efectos de los fármacos , Miometrio/metabolismo , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Células Madre Neoplásicas/efectos de los fármacos , Células Madre Neoplásicas/metabolismo , Neoplasias Uterinas/inducido químicamente , Neoplasias Uterinas/genética , Neoplasias Uterinas/metabolismo

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Compared with surgery, radiotherapy, chemotherapy and other conventional cancer treatments, near-infrared (NIR) photothermal therapy mediated by gold nanoparticles had advantages of non-invasive, safe, non-drug resistance and small side effects. Especially for tongue cancer and other head and neck tumors, NIR gold nanoparticle-mediated photothermal therapy is more conducive to preserve the anatomic and functional integrity of stomatognathic system, improving the life quality of patients. However, the toxic organic phase substances are commonly used in conventional gold nanoparticles preparation process in order to increase the stability of the material which limited its application in the biomedical field. In this study, a new type of gold nanoflower (GNF) particles were synthesized by seeding approach in aqueous phase, with better biocompatibility and free of organic substances. Then, the photo-thermal conversion curve under the irradiation of NIR laser ray were investigated. The absorption peak of prepared GNF particles was 719 nm and they had high efficient photothermal conversion in near-infrared region. Furthermore, we investigated the inhibition effect of NIR photothermal therapy with different laser power mediated by the GNF particles on human tongue carcinoma cells by detecting the relative growth rate, cell cycle, and apoptosis and necrosis. The results showed that NIR photothermal therapy mediated by prepared GNF particles could inhibit proliferation of tongue carcinoma Tca-8113 cells obviously, and the relative growth rate of Tca-8113 cells decreased along with the increasing of GNF concentration and laser power. At last, we validated the expression stabilities of 12 commonly used reference genes (18S, ACTB, ALAS1, GAPDH, TBP, HPRT1, RPL29, HMBS, PPIA, PUM1, GUSB, and B2M) after the above treatment through real-time quantitative polymerase chain reaction (RT-qPCR). Three commonly used software, geNorm, NormFinder, and BestKeeper are used to compare stability between reference genes. The final ranking of the candidate reference genes expression stability was TBP > PPIA > RPL29 > B2M > 18S rRNA > GAPDH > GUSB > ACTB > HPRT1 > ALAS1 > HMBS > PUM1. The combination of TBP + PPIA is best for normalizing the relative quantitation. The results suggested that GNF particles had high photothermal conversion in NIR region and was ideal mediated material for tumor NIR photothermal therapy. NIR photothermal therapy mediated by GNFs could inhibit the growth rate of tongue carcinoma Tca-8113 cells obviously. The recommend reference genes can be used for improving the accuracy of gene expression studies in the molecular mechanism of near-infrared (NIR) photothermal therapy mediated by the gold nanoflower (GNF) particles.

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Objective To investigate the impacts of emotion intelligence and sex difference on the process of emotion regulation.Methods The reaction time and the strength of emotion regulation were recorded in the emotion perception and emotion regulation task.Results The males with higher EI scores reacted more quickly in emotion perception and were more efficient in positive emotion regulation than those with lower EI scores (reaction time:(1 821±120) ms ＜ (2 320±133)ms,F(1.87)=3.87,P=0.049;strength of emotion regulation:2.11 ± 0.21 ＞ 0.81 ± 0.23;F(1,87) =17.31,P＜ 0.001),while the females with higher EI scores reacted more slowly in emotion perception and were more efficient in negative emotion regulation than those with lower EI scores (reaction time:(2 250± 110) ms ＞ (1 939± 115) ms,F(1,87) =6.73,P =0.011;strength of emotion regulation:1.60±0.18 ＞ 0.7±0.18;F(1,87)=12.12,P=0.001).Conclusion The results showed that for men,the EI was associated with efficiency in processing emotion and regulating positive emotions,while for women,the EI was associated with efficiency in regulating negative emotions.

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Objective To evaluate the impact of different surgical approach on postoperative renal function in patients with synchronous sporadic bilateral renal cell carcinoma ( SSBRCC) .Methods The medical records of 28 patients with SSBRCC were reviewed.Of all these cases, 21 patients were male and 7 were female, and the mean age was 51 ( 25-63 ) years.None of these cases had family history of renal carcinoma.The treatment and other clinical data were analyzed retrospectively.Results All of the 28 patients underwent bilateral surgeries.Single procedures were performed on 16 patients, of which, one underwent bilateral radical nephrectomy ( RN) , 8 bilateral nephron-sparing surgery ( NSS) and 7 unilateral NSS combined with contralateral RN.Staged procedures were performed on 12 patients, of which bilateral NSS was on 5 patients (NSS-NSS group), NSS followed by RN (NSS-RN group) on 3 patients and RN followed by NSS ( RN-NSS group) on 4 patients.In term of postoperative renal function preservation for SSBRCC patients, the standard NSS was better than RN.Between different staged procedures, NSS-NSS was the preferred choice, and the NSS-RN was superior to the RN-NSS.Conclusions In term of preservation of postoperative renal function, the staged procedures were recommended, and NSS was preferred in the first stage.In deciding the second stage surgical approaches, the surgeons should take the renal function preservation and the tumor size into consideration.

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<p><b>OBJECTIVE</b>To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining paeoniflorin and albiflorin in Paeoniae Radix Alba.</p><p><b>METHOD</b>Using paeoniflorin as the internal reference substance, the relative correction factor (RCF) of albiflorin was determined by HPLC and UPLC with good reproducibility. The contents of paeoniflorin in 16 samples of Paeoniae Radix Alba were authentically determined by the external standard method, and the content of albiflorin was calculated according to the RCF. The contents of these two components in the samples were determined with the external standard method.</p><p><b>RESULT</b>No siginificant differences between the quantitative results of QAMS method and external standard method were observe.</p><p><b>CONCLUSION</b>It is a convenient and accurate method to determine multi-components when some authentic standard substances were unavailable. It can be used to control the quality of Paeoniae Radix Alba</p>

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Benzoatos , Hidrocarburos Aromáticos con Puentes , Cromatografía Líquida de Alta Presión , Métodos , Glucósidos , Monoterpenos , Paeonia , Química

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<p><b>OBJECTIVE</b>To establish a HPLC method of characteristic chromatographic profile for the quality control of Paeoniae Radix.</p><p><b>METHOD</b>The 67 batches of samples were analyzed on a Polaris C18-A column with a gradient elution of acetonitrile and phosphate solution (pH 3.0) at a flow rate of 1.0 mL x min(-1) and detected at 230 nm.</p><p><b>RESULT</b>Nine main marker peaks were identified and semi-quantificated. By the similarity evaluation software for chromatographic fingerprint of traditional Chinese medicine (Version 2004A) and hierarchical clustering analysis, 67 batches of Paeoniae Radix were classified.</p><p><b>CONCLUSION</b>The method can be applied for quality assessment of Paeoniae Radix.</p>

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Cromatografía Líquida de Alta Presión , Métodos , Paeonia , Química , Control de Calidad

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<p><b>OBJECTIVE</b>To study the anti-platelet effect and influence of Radix Paeoniae Rubra and Radix Paeoniae Alba on rat's endogenous metabolites by animal experiment and UPLC-MS based metabolomic method.</p><p><b>METHOD</b>After administration of 80% ethanol extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba for 6 d, the serum samples were analyzed by ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) to find out the potential biomarker.</p><p><b>RESULT</b>Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition on platelet coacervation, and the effect of Radix Paeoniae Alba is better than that of Radix Paeoniae Rubra. Malic acid, alpha-acetone dicarboxylic acid, leukotrieneA4 (LTA4), prostaglandinE2 (PGE2) and prostaglandin F2alpha (PGF2alpha) are proved to be significant expressed biomarkers.</p><p><b>CONCLUSION</b>Metabolomics is helpful for the further research of the mechanism of anti-platelet action of Radix Paeoniae Rubra and Radix Paeoniae Alba.</p>

Asunto(s)

Animales , Masculino , Ratas , Cromatografía Liquida , Métodos , Análisis de los Mínimos Cuadrados , Espectrometría de Masas , Métodos , Metabolómica , Paeonia , Extractos Vegetales , Farmacología , Inhibidores de Agregación Plaquetaria , Farmacología , Ratas Sprague-Dawley

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<p><b>OBJECTIVE</b>To compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis.</p><p><b>METHOD</b>To establish the rats model of toes swelling caused by carrageenan, study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba. The serum samples were analyzed by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS), to find out the potential identification biomarker by PLS-DA.</p><p><b>RESULT</b>Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition to swelling caused by carrageenan in 0.5-1 h, and the extract of Radix Paeoniae Rubra also show significant inhibition in 2-3 h. Glutathione( GSH), gamma-aminobutyric acid (GABA), prostaglandin F2alpha (PGF2alpha), prostaglandinE3 (PGE3), leukotrieneA4 (LTA4), prostaglandinE2 ( PGE2) are proven to be significant expressed biomarkers. Radix Paeoniae Rubra and Radix Paeoniae Alba may have great influence on PGF2alpha and PGE3. There was also significant difference between the effects of Radix Paeoniae Rubra and Radix Paeoniae Alba, which suggested the difference of anti-inflammatory between the two herbs.</p><p><b>CONCLUSION</b>The results of metabolomics are related with the results of classic pharmaco- experiment, which is helpful for the further research of the mechanism of action of Radix Paeoniae Rubra and Radix Paeoniae Alba.</p>

Asunto(s)

Animales , Masculino , Ratas , Alprostadil , Sangre , Antiinflamatorios , Farmacología , Cromatografía Liquida , Métodos , Dinoprost , Sangre , Metabolómica , Paeonia , Extractos Vegetales , Farmacología , Ratas Sprague-Dawley , Espectrometría de Masa por Ionización de Electrospray , Métodos