Metabolomics study of anti-platelet effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by UPLC-MS / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 698-701, 2011.
Article
en Zh
| WPRIM
| ID: wpr-247406
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the anti-platelet effect and influence of Radix Paeoniae Rubra and Radix Paeoniae Alba on rat's endogenous metabolites by animal experiment and UPLC-MS based metabolomic method.</p><p><b>METHOD</b>After administration of 80% ethanol extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba for 6 d, the serum samples were analyzed by ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) to find out the potential biomarker.</p><p><b>RESULT</b>Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition on platelet coacervation, and the effect of Radix Paeoniae Alba is better than that of Radix Paeoniae Rubra. Malic acid, alpha-acetone dicarboxylic acid, leukotrieneA4 (LTA4), prostaglandinE2 (PGE2) and prostaglandin F2alpha (PGF2alpha) are proved to be significant expressed biomarkers.</p><p><b>CONCLUSION</b>Metabolomics is helpful for the further research of the mechanism of anti-platelet action of Radix Paeoniae Rubra and Radix Paeoniae Alba.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Espectrometría de Masas
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Inhibidores de Agregación Plaquetaria
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Extractos Vegetales
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Análisis de los Mínimos Cuadrados
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Cromatografía Liquida
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Ratas Sprague-Dawley
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Paeonia
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Metabolómica
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Métodos
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2011
Tipo del documento:
Article