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Pre-harvest sprouting is a frequent problem for wheat culture that can be simulated by laboratory-based germination. Despite reducing baking properties, wheat sprouting has been shown to increase the bioavailability of some nutrients. It was investigated whether wheat cultivars bearing distinct grain texture characteristics (BRS Guaraim, soft vs. BRS Marcante, hard texture) would have different behavior in terms of the changes in phytochemical compounds during germination. Using LC-Q-TOF-MS, higher contents of benzoxazinoids and flavonoids were found in the hard cultivar than in the soft one. Free phytochemicals, mainly benzoxazinoids, increased during germination in both cultivars. Before germination, soft and hard cultivars had a similar profile of matrix-bound phytochemicals, but during germination, these compounds have been shown to decrease only in the hard-texture cultivar, due to decreased levels of phenolic acids (trans-ferulic acid) and flavonoids (apigenin) that were bound to the cell wall through ester-type bonds. These findings confirm the hypothesis that hard and soft wheat cultivars have distinct behavior during germination concerning the changes in phytochemical compounds, namely the matrix-bound compounds. In addition, germination has been shown to remarkably increase the content of benzoxazinoids and the antioxidant capacity, which could bring a health-beneficial appeal for pre-harvested sprouted grains.

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Imidacloprid (IMI) is an insecticide used worldwide, a neonicotinoid that could cause toxicity in non-target organisms. Zebrafish (Danio rerio) is a model organism widely used in different fields of research such as behavioral studies, biochemical parameters as well as neurotoxicity research. Here, we investigate whether the exposure to three concentrations (0.15, 15, and 45 µg/L) of IMI for 96 h alters responses in zebrafish. Oxidative stress parameters and acetylcholinesterase activity (AChE) as well as the behavioral responses of locomotion were measured. IMI exposure decreased distance traveled in fish exposed to the 45 µg/L. In the exploratory activity, time spent and transitions to the top area of the water column decreased in fish exposed to all concentrations of IMI. In addition, exposures to 45 and 15 µg/L of IMI decreased episodes of erratic movement in the zebrafish. Exposures to IMI at a concentration of 45 µg/L decreased the time spent in erratic movements and increased the time spent with no movement (i.e., "freezing"). Glutathione S-transferase (GST) activity was increased in the brain of zebrafish exposed for 96 h to concentrations of 0.15 and 45 µg/L. Brain AChE activity was reduced and the levels of carbonyl protein (CP) increased in brain of zebrafish at concentrations of 15 and 45 µg/L. Lipid peroxidation measured by TBARS and, also non-protein thiols (NPSH) did not show any variation in the brain of zebrafish exposed to IMI. Changes in the activity of cholinergic neurotransmitters in the brain tissues of zebrafish indicate IMI toxicity. Exposures of fish over 96 h to IMI at a nominal concentration of 45 µg/L caused more extensive sublethal responses in zebrafish, but this concentration is well above those expected in the aquatic environment. Studies are warranted to evaluate the effects on behavior and biomarker responses in fish exposed over longer periods to IMI at environmentally relevant concentrations.

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Brazil is one of the largest consumers of pesticides in the world, and these chemicals present a high contamination risk for the country's water bodies. The mechanisms of mobilization and transport of pesticides from cropland to river systems are controlled by runoff and erosion processes occurring at the catchment scale. In addition to the excessive use of pesticides, the transport processes of these substances are also accelerated by inadequate soil management and the absence of soil conservation measures at the catchment scale. The current research relied on hydrological monitoring to investigate the transport and persistence of pesticides in response to hydrological dynamics. The study was conducted in the Conceição River watershed where runoff and suspended sediment fluxes are continuously monitored at the outlet. This study area is representative of the grain production system in southern Brazil including the application of large amounts of pesticides combined with extensive runoff and erosion problems. Sample collection in the river for pesticide analysis included the analysis of both water and suspended sediment. The sediment deposit analysis was performed in a single location at 4 depths. Results demonstrate the occurrence of pesticides including simazine, 2,4-D, carbendazim, imidacloprid, tebuconazole, propiconazole, tetraconazole, and trifloxystrobin in water, while glyphosate and AMPA were detected in suspended sediments, and AMPA and carbendazim were found in sediment deposits. The study demonstrated the strong dependence of the mechanisms of pesticide mobilization and transport in the catchment with the intra- and interevent variability of hydro-sedimentary processes. Pesticide detections can be related to several factors, including the magnitude of the rainfall event, the period of pesticide application, or the transport of suspended sediment. All these factors are correlated, and the mechanisms of transportation play an important role in the connections between sink and sources. The results suggest that pesticide monitoring should take into account the runoff and erosion pathways in each particular catchment, and it should especially include the monitoring of major rainfall events for identifying and quantifying the occurrence of pesticides in the environment. The transport of pesticides indicates to be potentiated by intensive pesticide use, the magnitude of rainfall-runoff events, and the absence of runoff control measures (e.g., terracing). These results demonstrate that water and soil conservation techniques should be planned and coordinated at the watershed scale to reduce the connectivity of water and sediment flows from agricultural areas to river systems with the implementation of effective runoff control practices. This will control the mobilization agents (runoff), as well as limit the connection between the sources and the water bodies.

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The high demand for food consequently increases the entry of agricultural residues into water resources, and this phenomenon can affect non-target organisms in different ways. Environmentally relevant pesticide effects (per se or in combinations) are scarce in the scientific literature. Therefore, the aim of this study was to investigate: (1) the presence of pesticide residues in an important Brazilian source of water supply and power generation (Jacuí river), during 1 year of monitoring. (2) in a laboratory study verify the effects of the most frequently, herbicide, fungicide, and insecticide found in Jacuí river (individualized or in a mixture) on biochemical parameters in different tissues of Oreochromis niloticus. Twenty pesticide residues were detected in superficial water samples, and two of them are banned in Brazilian territory. Atrazine (0.56 µg L-1), azoxystrobin (0.024 µg L-1), and imidacloprid (0.11 µg L-1) were the most frequently herbicide, fungicide, and insecticide, respectively, found in the river and were used in the laboratory assay. O. niloticus exposed to the pesticide mixture exhibited more biochemical effects than individualized exposure groups. This response can be a result of the combined pesticide effects, culminating in an additive or synergistic effect, depending on the biomarker. In individual exposure groups, atrazine presented the most pronounced alterations, followed by azoxystrobin and imidacloprid. Overall, pesticide exposure increased levels of oxidative stress parameters, reduced antioxidant enzyme activities, and induced acetylcholinesterase activity. These findings highlight the threat to aquatic organisms which may be exposed to a miscellaneous of toxic compounds in the environment.

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A wide variety of pharmaceuticals are discharged in water courses on a daily basis due to their incomplete removal from effluent in treatment plants. The aim of the current study was to assess the occurrence, fate and removal of pharmaceuticals from effluent and sludge samples collected in the biggest sanitary sewer plant in Southern Brazil. In total, 13 pharmaceuticals were detected in the influent through UHPLC-MS/M - paracetamol and caffeine recorded the highest concentrations, 137.98 and 35.29 µg L-1, respectively. The treated effluent presented 11 compounds. Antibiotics were the class recording the widest diversity; metronidazole showed the lowest concentration (0.023 µg L-1) and sulfamethoxazole presented the highest concentration (1.374 µg L-1) in influent samples. Seven pharmaceuticals were absorbed by the sludge; among them, one finds caffeine, ciprofloxacin and ofloxacin, which were quantified both in the effluent and in the sludge. On the other hand, doxycycline, fenbendazole, norfloxacin and tetracycline were only detected in sludge samples - their concentrations ranged from 0.026 to 5.034 mg kg-1. Clindamycin, oxytetracycline, sulfathiazole and trimethoprim concentrations increased throughout the treatment. There were high paracetamol and caffeine removal rates (>97%), and it may have happened due to degradation, photodegradation or chemical reaction. Ciprofloxacin and ofloxacin removal rate exceeded 83% mainly due to their sorption by sludge. Finally, the mass balance analysis highlighted high pharmaceutical loads (511.466 g d-1) discharged into recipient waterbodies. This outcome demands broadening the removal of these pharmaceuticals from sewage.

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Brazilian freshwater ecosystems are continuously exposed to pesticides and domestic sewage. The Uruguay River was chosen for this study because of its international importance, as it flows through Brazil, Argentina, and Uruguay. It receives contaminants such as pesticides and domestic residues. Thus, the aim of this study to assess the accumulation of pesticides in muscle of the fish Astyanax jacuhiensis, its biochemical responses, and the presence of pesticides in water. In total, seven pesticides were registered in water from both river sites. Eight pesticides were detected in fish muscle. The biochemical responses showed that brain lipid peroxidation (LPO) and protein carbonyl (PC) in A. jacuhiensis were higher in the summer. Muscle showed the highest LPO levels in the spring and the highest PC in the summer. Liver LPO and PC levels were higher in the spring and summer. In the gills, the PC was higher in the spring and the LPO in the spring and winter. In the brain and in the gills, glutathione-S-transferase activity was high in the summer and autumn. Catalase activity was lower during the winter and spring. Non-protein thiol (NPSH) levels were lower in the brain in the winter and spring. Muscle tissue showed lower NPSH in the winter (site 1). Liver NPSH showed increased levels in liver in the spring and winter (site 2). The biochemical results clearly is related to pesticides and/or to the presence of other contaminants in the water such as metals or domestic sewage. The accumulation of pesticides in fish muscle added evidence that pesticides have been used in the area surrounding the Uruguay River. In conclusion, the biomarkers assayed in the present study could be used in future investigations considering other sampling sites along Uruguay River.

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Sewage effluent effects on the biochemical parameters of Astyanax bimaculatus organs were investigateted. Treated sewage was collected in a treatment plant; 43 compounds, among them, pharmaceuticals and hormones, were investigated. Caffeine, ciprofloxacin, clindamycin, ofloxacin, oxytetracycline, paracetamol, sulfadiazine, sulfamethoxazole, sulfathiazole and tylosin waste was detected in the collected material. Fish were divided into four groups: control, TSE (treated sewage effluent), TSE + P (TSE with increased concentration of five pharmaceuticals) and PTSE (TSE + P post-treated with O3/H2O2/UV). Biochemical parameters were evaluated in different organs after 14-day exposure. TBARS levels increased significantly in the brain of animals in the TSE and TSE + P groups in comparison to the control. There was significant reduction in TBARS levels recorded for the liver, muscle and gills of animals in the PTSE group in comparison to those of animals in the other groups. AChE activity reduced in the muscle of animals in the groups showing the highest pharmaceutical concentrations. CAT activity in the liver of animals in groups exposed to pharmaceutical effluent was inhibited. GST activity increased in brain of animals in the TSE + P and PTSE groups, whereas reduced levels of this activity were observed in liver of animals in the TSE group. Increased GST activity was observed in the brain of animals in TSE + P and PTSE groups. Based on integrated biomarker response values, the TSE + P group presented greater changes in the analyzed parameters. Results point out that pharmaceutical waste can cause oxidative stress, as well as affect biochemical and enzymatic parameters in Astyanax sp. Post-treatment can also reduce damages caused to fish, even in case of the likely formation of metabolites. Based on these results, these metabolites can be less toxic than the original compounds; however, they were not able to fully degrade the pharmaceutical waste found in the sewage, which can interfere in fish metabolism.

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World food production is directly related to human population growth. Chemicals are constantly applied to pest control in crops to increase productivity. Therefore, sustainable alternatives are needed to reduce environmental impacts. The biochemical responses in liver and muscle of Astyanax lacustris collected in different rice planting systems were analysed. Ten fish were collected in organic rice cultivation systems and conventional as well as water and sediment for pesticide analysis. In water from conventional system, bentazon (56.1 µg L-1), fipronil (0.226 µg L-1) and propoxur (0.141 µg L-1) were found, while azoxystrobin and quinclorac were below the limit of quantification (LOQ). There were no pesticides in the sediment from the conventional system. In the water of the organic system, only propoxur (below the LOQ) was registered. Metalaxil (0.025 µg kg-1) were verified in the sediment, while diphenoconazole, dinoxifene and tebuconazole were below the LOQ. The presence of these pesticides in crops with an organic production system may be related to proximity to crops to conventional production systems. Besides this, the amount is very low as compared with conventional system. Muscle glycogen, protein and amino acid levels were higher in fish collected in organic ponds. Lactate and ammonia levels were higher in conventional cultures. Lipids and proteins had greater oxidative damage in both tissues in the conventional system. Although pesticides were detected in organic sediments, the parameters of metabolic and oxidative damage were probably related by exposure to higher concentrations of pesticide in the water or by the lower oxygen content of conventional systems. However, fish mortality was not observed during collections. Despite being an anthropic environment, areas of organic cultivation seem to present better conditions for the survival of A. lacustris.

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Reservoirs are lentic man-made waterbodies resulting from river damming processes. Pollutants coming from adjacent areas can accumulate in the water and sediment of these modified freshwater environments. Fish are often found in reservoirs occupying several trophic niches. Biochemical biomarkers are early warning signals of environmental disturbance to an organism. It is essential to understand how pollutants, abiotic variables and biochemical biomarker responses behave throughout the seasons to implement biomonitoring programs. Loricariichthys anus and Geophagus brasiliensis were collected, and abiotic variables were seasonally measured for one year, at six sampling sites in Passo Real reservoir, in a subtropical region of Southern Brazil. Biochemical biomarkers were analyzed in four tissues of both fish species, as well as metal and pesticide concentrations in the reservoir's water and sediment. Redundancy analysis (RDA) was carried out to find the temporal relationship between biomarkers and environmental variables. RDA has clearly shown the separation of seasons for both species. Azoxystrobin, simazine and propoxur were the pesticides mostly contributing to the variation, whereas metals had lesser contribution to it. Seasonality appears to be the main factor explaining biomarkers' variability. PERMANOVA has confirmed the effect of temperature and dissolved oxygen on biomarkers of both fish species. Thus, it is hard to differentiate if the fluctuation in biomarkers' responses only reflects the normal state of organisms or it is a biological consequence from negative effects of fish exposure to several types of pollution (sewage, pesticides, and fertilizers) entering this aquatic system. In this study, to circumvent the seasonality issue on biomonitoring, the analysis of biomarkers on these fish should not be carried out in organs directly affected by temperature (such as liver and gills), or during reproduction periods (mainly in Spring).

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Intensive agricultural and livestock activities demand high pesticide use and, consequently, contaminants reach aquatic ecosystems. In the lower Jacuí River, southern Brazil, there is a lack of knowledge about pesticide residues in water samples and the biochemical responses in native fish species. Thus, this study aimed to estimate the influence of pesticide residues and water parameters to biomarker responses in the native fish Astyanax spp. We performed seasonal biomonitoring in 2017 with water samples and fish collections. Biomarkers of oxidative stress, antioxidants, biotransformation, and neurotoxicity were analyzed in fish tissues. Fourteen pesticide residues were detected; they presented correlations with detoxification enzyme and oxidative stress biomarkers. These data indicate that most of variations can be related to the pesticide presence in water indicating high aquatic pollution in this place.

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The total cultivated area in Brazil reached to 62 million ha in 2018, with the predominance of genetically modified soybean and corn (36 and 17 million ha, respectively) in no-tillage systems. In 2018, 5.3 × 105 Mg of active ingredient of pesticides was applied in cropfields, representing about 7.3 L of commercial product by habitant. However, the monitoring of water courses contamination by pesticides remains scarce and is based on traditional grab sampling systems. In this study, we used the grab (water) and passive sampling (Polar Organic Chemical Integrative Sampler-POCIS) to monitor pesticide contamination in the river network of a representative agricultural catchment of southern Brazil. We selected 18 sampling sites located in tributaries and in the main course of the Guaporé River, in Rio Grande do Sul State, with different land use predominance including forest, urban, and agricultural areas. Altogether, 79 and 23 pesticides were, respectively, analyzed in water and POCIS samples. The water of Guaporé River and its tributaries were highly contaminated by many pesticides, especially by four herbicides (2,4-D, atrazine, deethyl-atrazine, and simazine), three fungicides (carbendazim, tebuconazole, and epoxiconazole), and one insecticide (imidacloprid). The amount, type, and concentration of pesticides detected were completely different depending on the sampling technic used. POCIS was effective to discriminate the contamination according to the main land use of each sampling site. The monitored areas with the predominance of soybean cultivation under no-tillage tended to have higher concentrations of fungicide, while in the more diversified region, the herbicides showed higher values. The presence of five herbicides used in corn and grassland forage production was correlated with areas of integrated crop-livestock systems, in contrast to higher contamination by 2,4-D in areas of intensive production of soybean and winter cereals.

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Aquatic environments are affected by the use of pesticides in agricultural areas near rivers. To assess the impact of pesticide residues on affected environments Danio rerio (zebrafish) embryos have become an alternative model for biomonitoring studies. In the present study, zebrafish embryos were used as bioindicator of water quality in the Vacacaí river, located in the city of Santa Maria, southern Brazil. We hypothesized that it would be possible to observe changes in the biomarkers tested in the embryos. Exposures were performed over a total of eight months during the year 2018 using water collected in a river located near agricultural areas. Twenty-four pesticides were found in river water samples. The most frequently found were atrazine, quinclorac and clomazone. During exposure (96 h) spontaneous movement, the heart rate and hatching rate were evaluated. After the exposure time the embryos were euthanized for biochemical assays. We analyzed biomarkers such as thiobarbituric acid reactive substance (TBARS), acetylcholinesterase (AChE), glutathione S-transferase (GST) and catalase (CAT). We observed increases in GST and TBARS, especially during periods of major water contamination such as January, February, October, and November. Pesticides can affect the development of native species that reproduce during periods of high agricultural production. These results demonstrate the potential use of biochemical parameters combined with developmental and behavioral analyses in zebrafish embryos for biomonitoring studies.

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The increasing need for animal protein has exerted pressures on the current animal production system. One of the alternatives found by producers to improve animal performance has been the use of veterinary drugs, especially antibiotics. However, its indiscriminate use can be a risk to the environmental balance of the producing locations since Brazil has approximately 42 million pigs. With this herd, pig farming represents a great potential for generating environmental impacts. As an alternative use of the large volume of liquid pig manure generated, many producers have been using this slurry in the soil as a bio fertilizer. Residues of veterinary drugs are capable of accumulation with environmental matrices and leach into water resources. In this sense, it is mandatory to dedicate more efforts on the study of techniques and processes for the treatment of organic effluents contaminated by veterinary drugs. Low-cost and environmentally friendly alternative treatment systems are necessary, in order to minimize the entry of these contaminants into the environment. Therefore, the composting process, defined as aerobic microbial decomposition process of organic matter, can be an alternative for treating the effluents contaminated by veterinary drugs. This review to aims to create awareness in the academic community regarding the veterinary drug residues and their contamination potential in different environmental matrices, as well as evaluating the composting process as a technique to minimize the impacts of the swine activity waste on the environment.

A crescente necessidade por proteína animal tem exercido pressões no atual sistema produtivo animal. Uma das alternativas encontradas pelos produtores para melhorar o desempenho animal tem sido a utilização de medicamentos veterinários, principalmente os antibióticos. No entanto, seu uso indiscriminado, pode ser um risco ao equilíbrio ambiental dos locais produtores, pois o Brasil possui aproximadamente 42 milhões de cabeça de suínos. Com este rebanho, a atividade suinícola representa um grande potencial de geração de impactos ambientais. Como alternativa de uso para o grande volume dos dejetos líquidos de suínos gerados, muitos produtores vêm utilizando o chorume no solo como biofertilizante. Os resíduos de medicamentos veterinários possuem capacidade de acumulação junto às matrizes ambientais, de serem lixiviados para os recursos hídricos. Neste sentido fazem-se necessários maiores estudos sobre técnicas e processos de tratamento de efluentes orgânicos contaminados por fármacos veterinários. Sistemas alternativos de tratamento de baixo custo e ambientalmente viáveis, são necessidades para a minimização da entrada no ambiente destes contaminantes. Logo, o processo de compostagem que pode ser definido como um processo de decomposição microbiana aeróbia da matéria orgânica pode ser uma alternativa para o tratamento dos efluentes contaminados por fármacos veterinários. O presente trabalho revisional tem como objetivo esclarecer a comunidade acadêmica quanto aos resíduos de medicamentos veterinários e sua potencialidade de contaminação nas diversas matrizes ambientais e avaliar a compostagem como técnica para a minimização dos impactos dos resíduos da atividade suinícola junto ao meio ambiente.

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The increasing need for animal protein has exerted pressures on the current animal production system. One of the alternatives found by producers to improve animal performance has been the use of veterinary drugs, especially antibiotics. However, its indiscriminate use can be a risk to the environmental balance of the producing locations since Brazil has approximately 42 million pigs. With this herd, pig farming represents a great potential for generating environmental impacts. As an alternative use of the large volume of liquid pig manure generated, many producers have been using this slurry in the soil as a bio fertilizer. Residues of veterinary drugs are capable of accumulation with environmental matrices and leach into water resources. In this sense, it is mandatory to dedicate more efforts on the study of techniques and processes for the treatment of organic effluents contaminated by veterinary drugs. Low-cost and environmentally friendly alternative treatment systems are necessary, in order to minimize the entry of these contaminants into the environment. Therefore, the composting process, defined as aerobic microbial decomposition process of organic matter, can be an alternative for treating the effluents contaminated by veterinary drugs. This review to aims to create awareness in the academic community regarding the veterinary drug residues and their contamination potential in different environmental matrices, as well as evaluating the composting process as a technique to minimize the impacts of the swine activity waste on the environment.(AU)

A crescente necessidade por proteína animal tem exercido pressões no atual sistema produtivo animal. Uma das alternativas encontradas pelos produtores para melhorar o desempenho animal tem sido a utilização de medicamentos veterinários, principalmente os antibióticos. No entanto, seu uso indiscriminado, pode ser um risco ao equilíbrio ambiental dos locais produtores, pois o Brasil possui aproximadamente 42 milhões de cabeça de suínos. Com este rebanho, a atividade suinícola representa um grande potencial de geração de impactos ambientais. Como alternativa de uso para o grande volume dos dejetos líquidos de suínos gerados, muitos produtores vêm utilizando o chorume no solo como biofertilizante. Os resíduos de medicamentos veterinários possuem capacidade de acumulação junto às matrizes ambientais, de serem lixiviados para os recursos hídricos. Neste sentido fazem-se necessários maiores estudos sobre técnicas e processos de tratamento de efluentes orgânicos contaminados por fármacos veterinários. Sistemas alternativos de tratamento de baixo custo e ambientalmente viáveis, são necessidades para a minimização da entrada no ambiente destes contaminantes. Logo, o processo de compostagem que pode ser definido como um processo de decomposição microbiana aeróbia da matéria orgânica pode ser uma alternativa para o tratamento dos efluentes contaminados por fármacos veterinários. O presente trabalho revisional tem como objetivo esclarecer a comunidade acadêmica quanto aos resíduos de medicamentos veterinários e sua potencialidade de contaminação nas diversas matrizes ambientais e avaliar a compostagem como técnica para a minimização dos impactos dos resíduos da atividade suinícola junto ao meio ambiente.(AU)

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Epilithic biofilms are communities of microorganisms composed mainly of microbial cells, extracellular polymeric substances from the metabolism of microorganisms, and inorganic materials. Biofilms are a useful tool to assess the impact of anthropic action on aquatic environments including the presence of pesticide residues such as glyphosate. The present work seeks to monitor the occurrence of glyphosate and AMPA residues in epilithic biofilms occurring in a watershed. For this, epilithic biofilm samples were collected in the Guaporé River watershed in the fall and spring seasons of 2016 at eight points. Physicochemical properties of the water and biofilms were determined. The determination of glyphosate and AMPA was performed using an ultra-high performance liquid chromatograph coupled to a tandem mass spectrometer. The concentrations of glyphosate and AMPA detected in epilithic biofilms vary with the season (from 90 to 305 µg kg-1 for glyphosate and from 50 to 240 µg kg-1 for AMPA, in fall and spring, respectively) and are strongly influenced by the amount of herbicide applications. Protected locations and those with poor access not demonstrate the presence of these contaminants. In the other seven points of the Guaporé River watershed, glyphosate was detected in concentrations ranging from 10 to 305 µg kg-1, and concentrations of AMPA ranged from 50 to 670 µg kg-1. An overview of the contamination in the Guaporé watershed shows that the most affected areas are located in the Marau sub-watershed, which are strongly influenced by the presence of the city of Marau. This confirms the indiscriminate use of glyphosate in the urban area (weed control, domestic gardens and horticulture) and constitutes a problem for human and animal health. The results showed that biofilms can accumulate glyphosate resulting from the contamination of water courses and are sensitive to the sources of pollution and pesticides present in rivers.

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The increasing need for animal protein has exerted pressures on the current animal production system. One of the alternatives found by producers to improve animal performance has been the use of veterinary drugs, especially antibiotics. However, its indiscriminate use can be a risk to the environmental balance of the producing locations since Brazil has approximately 42 million pigs. With this herd, pig farming represents a great potential for generating environmental impacts. As an alternative use of the large volume of liquid pig manure generated, many producers have been using this slurry in the soil as a bio fertilizer. Residues of veterinary drugs are capable of accumulation with environmental matrices and leach into water resources. In this sense, it is mandatory to dedicate more efforts on the study of techniques and processes for the treatment of organic effluents contaminated by veterinary drugs. Low-cost and environmentally friendly alternative treatment systems are necessary, in order to minimize the entry of these contaminants into the environment. Therefore, the composting process, defined as aerobic microbial decomposition process of organic matter, can be an alternative for treating the effluents contaminated by veterinary drugs. This review to aims to create awareness in the academic community regarding the veterinary drug residues and their contamination potent

A crescente necessidade por proteína animal tem exercido pressões no atual sistema produtivo animal. Uma das alternativas encontradas pelos produtores para melhorar o desempenho animal tem sido a utilização de medicamentos veterinários, principalmente os antibióticos. No entanto, seu uso indiscriminado, pode ser um risco ao equilíbrio ambiental dos locais produtores, pois o Brasil possui aproximadamente 42 milhões de cabeça de suínos. Com este rebanho, a atividade suinícola representa um grande potencial de geração de impactos ambientais. Como alternativa de uso para o grande volume dos dejetos líquidos de suínos gerados, muitos produtores vêm utilizando o chorume no solo como biofertilizante. Os resíduos de medicamentos veterinários possuem capacidade de acumulação junto às matrizes ambientais, de serem lixiviados para os recursos hídricos. Neste sentido fazem-se necessários maiores estudos sobre técnicas e processos de tratamento de efluentes orgânicos contaminados por fármacos veterinários. Sistemas alternativos de tratamento de baixo custo e ambientalmente viáveis, são necessidades para a minimização da entrada no ambiente destes contaminantes. Logo, o processo de compostagem que pode ser definido como um processo de decomposição microbiana aeróbia da matéria orgânica pode ser uma alternativa para o tratamento dos efluentes contaminados p

RESUMEN

The increasing need for animal protein has exerted pressures on the current animal production system. One of the alternatives found by producers to improve animal performance has been the use of veterinary drugs, especially antibiotics. However, its indiscriminate use can be a risk to the environmental balance of the producing locations since Brazil has approximately 42 million pigs. With this herd, pig farming represents a great potential for generating environmental impacts. As an alternative use of the large volume of liquid pig manure generated, many producers have been using this slurry in the soil as a bio fertilizer. Residues of veterinary drugs are capable of accumulation with environmental matrices and leach into water resources. In this sense, it is mandatory to dedicate more efforts on the study of techniques and processes for the treatment of organic effluents contaminated by veterinary drugs. Low-cost and environmentally friendly alternative treatment systems are necessary, in order to minimize the entry of these contaminants into the environment. Therefore, the composting process, defined as aerobic microbial decomposition process of organic matter, can be an alternative for treating the effluents contaminated by veterinary drugs. This review to aims to create awareness in the academic community regarding the veterinary drug residues and their contamination potent

A crescente necessidade por proteína animal tem exercido pressões no atual sistema produtivo animal. Uma das alternativas encontradas pelos produtores para melhorar o desempenho animal tem sido a utilização de medicamentos veterinários, principalmente os antibióticos. No entanto, seu uso indiscriminado, pode ser um risco ao equilíbrio ambiental dos locais produtores, pois o Brasil possui aproximadamente 42 milhões de cabeça de suínos. Com este rebanho, a atividade suinícola representa um grande potencial de geração de impactos ambientais. Como alternativa de uso para o grande volume dos dejetos líquidos de suínos gerados, muitos produtores vêm utilizando o chorume no solo como biofertilizante. Os resíduos de medicamentos veterinários possuem capacidade de acumulação junto às matrizes ambientais, de serem lixiviados para os recursos hídricos. Neste sentido fazem-se necessários maiores estudos sobre técnicas e processos de tratamento de efluentes orgânicos contaminados por fármacos veterinários. Sistemas alternativos de tratamento de baixo custo e ambientalmente viáveis, são necessidades para a minimização da entrada no ambiente destes contaminantes. Logo, o processo de compostagem que pode ser definido como um processo de decomposição microbiana aeróbia da matéria orgânica pode ser uma alternativa para o tratamento dos efluentes contaminados p

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In this study, a GnRH agonist, leuprolide acetate (LA), was given as a single depot injection before 48 h of life to Wistar female rats allotted to prenatal (E16-18) and postnatal androgenization (day 5 of life) by the use of testosterone propionate, looking for reproductive endpoints. Remarkably, a single injection of LA increased the estrus cycles in the postnatal group (PostN) from 0% to 25% of the estrus cycles in the postnatal LA treated group (PostN L). LA also reduced the serum testosterone levels and cysts and atretic follicles in PostN L in contrast with rats (>100 days) from the PostN group (p = 0.04). Prenatally androgenized rats (PreN) exhibited significant modifications in the hypothalamic genes, such as Gnrh. To the best of our knowledge, this is the first study to show that blockage of the GnRH axis with leuprolide acetate depot prevented the development of typical features (anovulation, cysts, atretic follicles) in a postnatal testosterone propionate rat model of PCOS.

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The use of pesticides is directly linked to improvements in productivity and to the preservation of coconut palms. However pesticide analysis is necessary to determine whether pesticide residues in the food products containing coconut are within the maximum residue limits (MRLs), ensuring the quality of these products. This work aimed to develop a method for multiresidue determination of ten pesticides in coconut water and pulp using QuEChERS and LC-MS/MS. The method was effective in terms of selectivity, linearity, matrix effect, accuracy and precision, providing LOD of 3µgkg(-1), LOQ of 10µgkg(-1) and recoveries between 70 and 120% with RSD lower than 20%. The developed method was applied to 36 samples in which residues of carbendazim, carbofuran, cyproconazole and thiabendazole were found below the LOQ in coconut water and pulp.

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