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The impact of postnatal leuprolide acetate treatment on reproductive characteristics in a rodent model of polycystic ovary syndrome.
Serrano Mujica, Lady Katerine; Bertolin, Kalyne; Bridi, Alessandra; Glanzner, Werner Giehl; Rissi, Vitor Braga; de Camargo, Flávia de Los Santos; Zanella, Renato; Prestes, Osmar Damian; Moresco, Rafael Noal; Antoniazzi, Alfredo Quites; Dias Gonçalves, Paulo Bayard; Premaor, Melissa Orlandin; Comim, Fabio Vasconcellos.
Afiliación
  • Serrano Mujica LK; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Bertolin K; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Bridi A; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Glanzner WG; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Rissi VB; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • de Camargo FL; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Zanella R; Laboratory of Pesticide Residue Analysis-LARP, Chemistry Department, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Prestes OD; Laboratory of Pesticide Residue Analysis-LARP, Chemistry Department, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Moresco RN; Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Antoniazzi AQ; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Dias Gonçalves PB; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Premaor MO; Department of Clinical Medicine, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
  • Comim FV; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil; Department of Clinical Medicine, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: fcomim@ufsm.br.
Mol Cell Endocrinol ; 442: 125-133, 2017 02 15.
Article en En | MEDLINE | ID: mdl-27988272
In this study, a GnRH agonist, leuprolide acetate (LA), was given as a single depot injection before 48 h of life to Wistar female rats allotted to prenatal (E16-18) and postnatal androgenization (day 5 of life) by the use of testosterone propionate, looking for reproductive endpoints. Remarkably, a single injection of LA increased the estrus cycles in the postnatal group (PostN) from 0% to 25% of the estrus cycles in the postnatal LA treated group (PostN L). LA also reduced the serum testosterone levels and cysts and atretic follicles in PostN L in contrast with rats (>100 days) from the PostN group (p = 0.04). Prenatally androgenized rats (PreN) exhibited significant modifications in the hypothalamic genes, such as Gnrh. To the best of our knowledge, this is the first study to show that blockage of the GnRH axis with leuprolide acetate depot prevented the development of typical features (anovulation, cysts, atretic follicles) in a postnatal testosterone propionate rat model of PCOS.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndrome del Ovario Poliquístico / Reproducción / Leuprolida Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Cell Endocrinol Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Irlanda

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndrome del Ovario Poliquístico / Reproducción / Leuprolida Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Cell Endocrinol Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Irlanda