RESUMEN
The results of screening more than 23,000 serum samples from persons belonging to risk groups, as well as those not belonging to such groups, in Moscow, Vilnius and Klaipeda are presented. Screening was carried out with the use of an assay system manufactured by the Scientific and Industrial Amalgamation "Antigen" (USSR). In this screening 3 HIV carriers were detected; of these, 2 were foreign students from two African countries.
Asunto(s)
Síndrome de Inmunodeficiencia Adquirida/prevención & control , Portador Sano/prevención & control , Anticuerpos Anti-VIH/sangre , VIH-1/inmunología , Tamizaje Masivo/métodos , Síndrome de Inmunodeficiencia Adquirida/epidemiología , Síndrome de Inmunodeficiencia Adquirida/inmunología , Donantes de Sangre , Portador Sano/epidemiología , Portador Sano/inmunología , Ensayo de Inmunoadsorción Enzimática , Seropositividad para VIH/epidemiología , Seropositividad para VIH/inmunología , Humanos , Immunoblotting , Lituania/epidemiología , Moscú/epidemiología , Factores de RiesgoAsunto(s)
Serodiagnóstico del SIDA/métodos , Serodiagnóstico del SIDA/instrumentación , Electroforesis en Gel de Poliacrilamida/instrumentación , Electroforesis en Gel de Poliacrilamida/métodos , Estudios de Evaluación como Asunto , Reacciones Falso Negativas , Reacciones Falso Positivas , Técnica del Anticuerpo Fluorescente , Productos del Gen gag/inmunología , Anticuerpos Anti-VIH/sangre , Humanos , Immunoblotting/instrumentación , Immunoblotting/métodos , Precursores de Proteínas/inmunología , Productos del Gen gag del Virus de la Inmunodeficiencia HumanaRESUMEN
One of the variants of IFA using a conjugate on the basis of anti-HIV-IgG was used for control of the content of human immunodeficiency virus (HIV) antigen at different stages of production of a test system for serodiagnosis of AIDS. This method permits HIV antigen quantitation in virus lysates, its nonpurified concentrates, and in native culture fluid in titres from 2000 to 60,000 and from less than 27 to greater than 729, respectively. In most cases, high titres of antigen in the liquid fraction of the culture corresponded to high values of the antigen-containing cells on the basis of immunofluorescence data and signs of intensive HIV production in electron microscopic culture control. The authors developed a method allowing to determine the antigen titre in the study material using only one dilution (1:9) of it in IFA technique.
Asunto(s)
Serodiagnóstico del SIDA/métodos , Antígenos VIH/análisis , Juego de Reactivos para Diagnóstico , Técnica del Anticuerpo Fluorescente , Humanos , Inmunoglobulina G/análisis , Inmunoglobulina G/inmunologíaRESUMEN
An increase in values of both kinetic parameters (Km and Vmax) of the delta-aminolevulinate dehydratase reaction was observed in blood of rats poisoned with lead. The Km value of the reaction was increased, while Vmax--decreased, after addition of Pb2+ into rat blood in vitro. The results obtained as well as the data published in literature suggest that a compensatory mechanism appears to function in vivo, where a substrate, delta-aminolevulenic acid, plays a triggering role.