RESUMEN
The development of colorectal cancer involves some morphological changes, and in the initial stage, pre-neoplastic lesions called aberrant crypt foci (ACF) appear. Thus, an intervention with sources of bioactive compounds such as Hibiscus sabdariffa L., rich in phenolic compounds and anthocyanins, could attenuate the risk of developing these lesions due to its antioxidant, anti-inflammatory and anti-proliferative properties. Therefore, the aim of this study was to evaluate the effects of 5% and 10% supplementation of dehydrated H. sabdariffa calyces (DHSC) during the development of 1,2-dimethylhydrazine-induced preneoplastic lesions in male BALB/c mice. The characterization of DHSC was carried out. The in vivo experiment lasted 12 weeks, and the animals were randomly divided into 3 experimental groups: the control group (CON) and the supplemented groups with 5% DHSC and 10% DHSC. The activities of liver enzymes catalase and superoxide dismutase were determined. In addition, ACF, short chain fatty acids (SCFA), presence of inflammatory infiltrates, goblet cells and leukocytes in the colonic mucosa were quantified. There was a significant reduction in ACF and the presence of inflammatory infiltrates in the colon of animals in groups 5DHSC and 10DHSC. In addition, the 10DHSC group showed an increase in the activity of the catalse enzyme, in the production of butyrate and in the presence of NK cells in the colon, in addition to more hypertrophied goblet cells. Based on these findings, it is suggested that DHSC supplementation may be recommended to attenuate cellular responses in the early stage of preneoplastic lesions.
RESUMEN
This systematic review analyzes the methodologies of Brazilian studies that have used indices to evaluate dietary quality. A systematic search was performed of electronic databases (Lilacs, Medline, SciELO and Scopus) with no restriction on the year of publication of the studies. Original, Brazilian articles were selected that assessed dietary quality using the Healthy Eating Index (HEI) or its revised versions. The descriptors were as follows: dietary quality; healthy eating index; and dietary quality index. Atotal of 45 articles were selected, of which 60% analyzed dietary quality using an instrument called the Healthy Eating Index and the rest used an index of dietary quality. Of the analyzed studies, 68.9% classified dietary quality. Most of the studies used ten items to evaluate dietary quality and were not standardized regarding methodologies. A total of 33.3% of the studies related the index to socioeconomic and demographic factors, food items and health conditions. Differences in terms of nomenclature and methodologies made it difficult to compare these studies of dietary quality.
Asunto(s)
Dieta Saludable , Dieta/normas , Brasil , Humanos , Evaluación Nutricional , Factores SocioeconómicosRESUMEN
Resumo Esta revisão sistemática objetivou analisar metodologias de estudos brasileiros que utilizam índices para avaliação da qualidade da dieta. Realizou-se busca sistemática em bases eletrônicas de dados (Lilacs, Medline, SciELO e Scopus), sem fazer restrição ao ano de publicação dos estudos. Foram selecionados artigos originais, nacionais, que avaliassem a qualidade da dieta pelo Healthy Eating Index (HEI) ou suas versões revisadas. Utilizou-se os descritores: qualidade da dieta, índice de alimentação saudável, índice de qualidade da dieta e respectivos termos em inglês. Foram selecionados 45 artigos, dos quais, 60% analisaram a qualidade da dieta por instrumento denominado índice de alimentação saudável e o restante por índice de qualidade da dieta. Dos estudos analisados, 68,9% classificaram a qualidade da dieta. A maioria dos estudos utilizou 10 itens para avaliação da qualidade da dieta e não apresentaram padronização quanto às metodologias. Dos estudos, 33,3% relacionaram o índice com fatores socioeconômicos e demográficos, itens alimentares e condições de saúde. Diferenças de nomeação e metodologias dificultam comparações entre os estudos de avaliação da qualidade da dieta.
Abstract This systematic review analyzes the methodologies of Brazilian studies that have used indices to evaluate dietary quality. A systematic search was performed of electronic databases (Lilacs, Medline, SciELO and Scopus) with no restriction on the year of publication of the studies. Original, Brazilian articles were selected that assessed dietary quality using the Healthy Eating Index (HEI) or its revised versions. The descriptors were as follows: dietary quality; healthy eating index; and dietary quality index. Atotal of 45 articles were selected, of which 60% analyzed dietary quality using an instrument called the Healthy Eating Index and the rest used an index of dietary quality. Of the analyzed studies, 68.9% classified dietary quality. Most of the studies used ten items to evaluate dietary quality and were not standardized regarding methodologies. A total of 33.3% of the studies related the index to socioeconomic and demographic factors, food items and health conditions. Differences in terms of nomenclature and methodologies made it difficult to compare these studies of dietary quality.
Asunto(s)
Humanos , Dieta/normas , Dieta Saludable , Factores Socioeconómicos , Brasil , Evaluación NutricionalRESUMEN
BACKGROUND: The only treatment for celiac disease is the gluten-free diet. Few studies have assessed the nutritional adequacy of this diet, especially of B vitamins related to homocysteine metabolism. The aim of this study was to assess the nutritional status and serum concentrations of B vitamins involved in homocysteine metabolism, and to determine whether the dietary intake of these vitamins are meeting Dietary Reference Intakes in celiac patients. METHODS: A cross-sectional study enrolled a total of 20 celiac patients (36.3 ± 13.7 years old; 65% women), following strict gluten-free diet (GFD) and 39 healthy controls matched by sex and age. The dietary intake was assessed by 3-day food records, and serum concentrations of homocysteine and vitamins B6, B12, and folate were determined after overnight fasting. Comparisons between the two groups were performed by Student's t test or Mann-Whitney U-test, for continuous variables. Pearson's chi-square test or Fisher's exact test was used for categorical variables. An alpha level of 5% were considered significant. RESULTS: Celiac patients had lower serum folate concentrations (7.7 ± 3.5 ng/mL, P < 0.05) than controls. All celiac patients had folate intake below the Estimated Average Requirement (EAR) (130.8 ± 53.6 µg/d). However, only a small proportion of celiac patients had hyperhomocysteinemia. CONCLUSIONS: Celiac patients treated with GFD presented inadequacy of dietary folate intake and low-serum concentrations of folate, suggesting that more attention should be given to the quality of the nutrients offered by the GFD, as it constitutes a lifelong treatment.
Asunto(s)
Enfermedad Celíaca/sangre , Ácido Fólico/sangre , Homocisteína/sangre , Vitamina B 12/sangre , Vitamina B 6/sangre , Complejo Vitamínico B/sangre , Adulto , Enfermedad Celíaca/dietoterapia , Estudios Transversales , Registros de Dieta , Dieta Sin Gluten , Femenino , Humanos , Masculino , Necesidades Nutricionales , Estado NutricionalRESUMEN
PURPOSE: Evaluate the effect of flaxseed, olive and fish oil on the lipid profile, preservation of villosities and lymphocyte migration in the intestinal mucosa of Wistar rats. METHODS: Thirty Wistar male rats were divided into four groups, which received the AIN-93M diet, with changes only to their lipid source: flaxseed, olive, fish, and soy oil (control group). The serum was separated for the biochemical parameter analysis. A histological evaluation was performed in the ileal portion. RESULTS: The group which was fed fish oil presented lower values when compared to the other treatments for Total Cholesterol, High-density Lipoprotein Cholesterol and Triacylglycerol (p<0.05). The animals treated with fish and olive oils presented better intestinal villosities preservation. Less deposition of lymphocytes was observed in the flaxseed group (p<0.001). CONCLUSIONS: This study demonstrated that flaxseed, olive and fish oils present different responses than soy oil for the intestinal mucosa preservation and lymphocyte proliferation in Wistar rats.
OBJETIVO: Avaliar o efeito dos óleos de linhaça, oliva e peixe no perfil lipídico, preservação das vilosidades e migração de linfócitos na mucosa intestinal de ratos Wistar. MÉTODOS: Trinta ratos Wistar foram divididos em quarto grupos e receberam dieta AIN-93M, modificando para cada grupo apenas a fonte lipídica: óleo de linhaça, oliva, peixe e soja ( grupo controle). O soro foi separado para análise dos parâmetros bioquímicos. A análise histológica foi realizada na porção ileal. RESULTADOS: O grupo que recebeu óleo de peixe apresentou menores valores de colesterol total, lipoproteína de alta densidade e triacilglicerol (p<0.05). Os animais tratados com óleo de peixe e oliva apresentaram melhor preservação das vilosidades intestinais. Menor deposição de linfócitos foi observado no grupo tratado com óleo de linhaça (p<0.001). CONCLUSÃO: Este estudo demonstrou que os óleos de linhaça, oliva e peixe apresentam diferentes respostas em relação ao óleo de soja na preservação da mucosa intestinal e proliferação de linfócitos em ratos Wistar.
Asunto(s)
Animales , Masculino , Ratas , Mucosa Intestinal/metabolismo , Metabolismo de los Lípidos , Lípidos/sangre , Linfocitos/metabolismo , Análisis de Varianza , Movimiento Celular/efectos de los fármacos , Grasas de la Dieta/metabolismo , Grasas de la Dieta/farmacología , Aceites de Pescado/metabolismo , Aceites de Pescado/farmacología , Mucosa Intestinal/efectos de los fármacos , Aceite de Linaza/metabolismo , Aceite de Linaza/farmacología , Lípidos/análisis , Linfocitos/efectos de los fármacos , Modelos Animales , Aceites de Plantas/metabolismo , Aceites de Plantas/farmacología , Ratas Wistar , Aceite de Soja/metabolismo , Aceite de Soja/farmacologíaRESUMEN
PURPOSE: Evaluate the effect of flaxseed, olive and fish oil on the lipid profile, preservation of villosities and lymphocyte migration in the intestinal mucosa of Wistar rats. METHODS: Thirty Wistar male rats were divided into four groups, which received the AIN-93M diet, with changes only to their lipid source: flaxseed, olive, fish, and soy oil (control group). The serum was separated for the biochemical parameter analysis. A histological evaluation was performed in the ileal portion. RESULTS: The group which was fed fish oil presented lower values when compared to the other treatments for Total Cholesterol, High-density Lipoprotein Cholesterol and Triacylglycerol (p<0.05). The animals treated with fish and olive oils presented better intestinal villosities preservation. Less deposition of lymphocytes was observed in the flaxseed group (p<0.001). CONCLUSIONS: This study demonstrated that flaxseed, olive and fish oils present different responses than soy oil for the intestinal mucosa preservation and lymphocyte proliferation in Wistar rats.(AU)
OBJETIVO: Avaliar o efeito dos óleos de linhaça, oliva e peixe no perfil lipídico, preservação das vilosidades e migração de linfócitos na mucosa intestinal de ratos Wistar. MÉTODOS: Trinta ratos Wistar foram divididos em quarto grupos e receberam dieta AIN-93M, modificando para cada grupo apenas a fonte lipídica: óleo de linhaça, oliva, peixe e soja ( grupo controle). O soro foi separado para análise dos parâmetros bioquímicos. A análise histológica foi realizada na porção ileal. RESULTADOS: O grupo que recebeu óleo de peixe apresentou menores valores de colesterol total, lipoproteína de alta densidade e triacilglicerol (p<0.05). Os animais tratados com óleo de peixe e oliva apresentaram melhor preservação das vilosidades intestinais. Menor deposição de linfócitos foi observado no grupo tratado com óleo de linhaça (p<0.001). CONCLUSÃO: Este estudo demonstrou que os óleos de linhaça, oliva e peixe apresentam diferentes respostas em relação ao óleo de soja na preservação da mucosa intestinal e proliferação de linfócitos em ratos Wistar.(AU)
Asunto(s)
Ratas , Aceite de Linaza/administración & dosificación , Aceite de Linaza/efectos adversos , Aceites de Pescado/administración & dosificación , Aceites de Pescado/efectos adversos , Grasas Vegetales , Ratas , Dieta , Dieta , Linfocitos/microbiología , Mucosa IntestinalRESUMEN
PURPOSE: Evaluate the effect of flaxseed, olive and fish oil on the lipid profile, preservation of villosities and lymphocyte migration in the intestinal mucosa of Wistar rats. METHODS: Thirty Wistar male rats were divided into four groups, which received the AIN-93M diet, with changes only to their lipid source: flaxseed, olive, fish, and soy oil (control group). The serum was separated for the biochemical parameter analysis. A histological evaluation was performed in the ileal portion. RESULTS: The group which was fed fish oil presented lower values when compared to the other treatments for Total Cholesterol, High-density Lipoprotein Cholesterol and Triacylglycerol (p<0.05). The animals treated with fish and olive oils presented better intestinal villosities preservation. Less deposition of lymphocytes was observed in the flaxseed group (p<0.001). CONCLUSIONS: This study demonstrated that flaxseed, olive and fish oils present different responses than soy oil for the intestinal mucosa preservation and lymphocyte proliferation in Wistar rats.