RESUMEN
The objectives of this study were to evaluate the influence of diet composition on ruminal parameters, more particularly redox potential (Eh). Four Holstein dry dairy cows, fitted with ruminal cannulas, were allocated in a 4 × 4 Latin square design. They were given four experimental hay-based diets D0, D25, D42 and D56 consisting of 0%, 25%, 42% and 56% of ground wheat and barley concentrate mixture, respectively. They were fed at a daily feeding rate of 8.0 kg DM per cow during a 24-day experimental period (a 21-day diet adaptation, three consecutive days of measurement and sampling). The physicochemical parameters, such as pH and Eh, were measured and Clark's exponent (rH) was calculated from 1 h before feeding to 8 h after feeding at 1-h interval. Samples of ruminal fluid were taken at 0, 1, 2, 4, 6 and 8 h after feeding for the determination of volatile fatty acid (VFA) and ammonia N (NH3-N) concentrations. Ruminal bacterial populations were also studied by means of capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) technique to focus on the structure of the ruminal microbiota and the diversity index was calculated. Mean ruminal Eh and rH were not modified by the concentrate-to-forage ratio and averaged - 210 mV and 6.30, respectively, across diets. The pH decreased slightly by 0.10 pH unit between treatments D0 and D56 with an average of 6.58. Nevertheless, the time during which physicochemical parameters remained at nadir value after feeding varied with diets: 2 and 7 h for D0 and 6 and 5 h for D56, respectively for pH and Eh. Moreover, fermentative parameters were altered by treatments: total VFA and NH3-N were greater in D56 (72.2 mM and 17.5 mg/100 ml) compared with D0 (65.2 mM and 14.2 mg/100 ml). However, neither the structure of bacterial populations of the rumen nor the diversity index (Shannon) was altered by treatments.
RESUMEN
The objectives of this study were to evaluate the capacity of 2 dietary feed additives, sodium bicarbonate and live yeast Saccharomyces cerevisiae (strain Sc 47), in optimizing ruminal pH in dairy cows and to determine their modes of action. Three early lactating Holstein cows, fitted with ruminal cannulas, were allocated in a 3 x 3 Latin square design. They were given a total mixed ration as control diet (CD) at a daily feeding rate of 28.0 kg of dry matter (DM)/cow supplemented with 150 g/d of sodium bicarbonate (SBD) or 5 g/d of live yeast (YD) during a 21-d experimental period (14 d of diet adaptation, 4 consecutive days of measurement and sampling and 3 d of transition). The pH and redox potential (E(h)) were measured from 1 h before feeding to 8 h after feeding at 1-h intervals, and samples of ruminal fluid were taken at 0, 2, 4, 6, and 8 h after feeding for the determination of volatile fatty acids and lactate concentrations. Total tract apparent digestibility of the diet was also determined. Ruminal pH fluctuated between 6.53 at feeding and 5.57 at 5 h postfeeding. Mean pH was greater with SBD (6.21) and YD (6.14) compared with CD (5.94), showing that both additives had a pH stabilization effect. The E(h) varied from -88 mV at 1 h before feeding to -165 mV at 1 h after feeding. Mean E(h) and Clark's Exponent (rH) were lower with YD (-149 mV and 7.31, respectively) than with SBD (-137 mV and 7.85, respectively) and CD (-115 mV and 8.05, respectively), indicating that the yeast strengthened the reducing power of the milieu. Total volatile fatty acids were greater in SBD (95.3 mM) and YD (99.4 mM) compared with CD (85.3 mM). Acetate concentration was greater in SBD (60.8 mM) and YD (59.1 mM) compared with CD (53.2 mM). Propionate concentration was greater in YD (25.8 mM) than in SBD (20.0 mM) and CD (18.0 mM). Butyrate remained constant between diets. Mean total lactate concentrations were 16.5, 12.2, and 5.4 mM for CD, SBD, and YD, respectively, with a 67% decrease with YD. Total tract organic matter digestibility was greater for YD (66.6%) compared with SBD (61.7%) and CD (62.2%). The neutral detergent fiber digestibility was greater with YD (41.6%) compared with SBD (34.3%) and CD (29.6%), whereas acid detergent fiber digestibility was greatest in YD (32.3%), intermediate in SBD (24.4%), and lowest in CD (18.1%). By inducing a lower ruminal E(h) and rH, live yeast prevented accumulation of lactate and allowed better fiber digestion, whereas sodium bicarbonate seemed to act only as an exogenous buffer.
Asunto(s)
Bovinos/fisiología , Industria Lechera , Rumen/efectos de los fármacos , Saccharomyces cerevisiae/fisiología , Bicarbonato de Sodio/farmacología , Animales , Bovinos/metabolismo , Dieta/veterinaria , Digestión/efectos de los fármacos , Ácidos Grasos Volátiles/metabolismo , Femenino , Fermentación/efectos de los fármacos , Fermentación/fisiología , Concentración de Iones de Hidrógeno , Ácido Láctico/metabolismo , Oxidación-Reducción/efectos de los fármacos , Rumen/química , Rumen/metabolismo , Rumen/microbiologíaRESUMEN
A sampling and measuring device was set up to measure continuously the pH and the redox potential (Eh) of ruminal content in absence of any gaseous contamination (method 1). It was compared with a conventional suction device in which no precaution was taken to prevent air from coming into contact with the surface of collected samples (method 2). Two fistulated dry cows were used and fed a total mixed ration. Redox potential and pH measurements were performed repeatedly on collected samples using these 2 methods during a 9-h period; each period started 1 h before feeding. The partial pressure of oxygen (log fO2) was calculated from Nernst's equation using pH and Eh values. Results indicated that pH, Eh, and log f(O2) were affected by sampling method. In method 1, pH values ranged from 6.7 to 6.37 and Eh from -173.5 to -216.8 mV. In method 2, pH and Eh values varied, respectively, from 6.93 to 6.49 and from -111.3 to -139.5 mV. The partial pressure of oxygen was 10(6) times lower in samples that were continuously collected than in hand-samples. As a result, method 1 could make accurate measurements of pH and Eh of ruminal content.
Asunto(s)
Bovinos/metabolismo , Industria Lechera/instrumentación , Rumen/química , Animales , Dieta , Femenino , Concentración de Iones de Hidrógeno , Cinética , Oxidación-Reducción , Oxígeno/administración & dosificación , Control de Calidad , Manejo de Especímenes/métodos , Manejo de Especímenes/veterinariaRESUMEN
Three experiments were conducted by in vitro incubations in ruminal fluid to investigate the effects of pH and amounts of linoleic and linolenic acids on the extent of their biohydrogenation, the proportions of conjugated linoleic acid (CLA) and trans-C18:1 as intermediates, and the ratio trans-10:trans-11 intermediates. The effects of pH and amount of linoleic acid were investigated in kinetic studies, and effects of the amount of linolenic acid were studied with 6-h incubations. With identical initial amounts of linoleic acid, its disappearance declined when the mean pH during incubation was under 6.0 compared with a mean pH over 6.5, and when the amount of linolenic acid increased from 10 to 180 mg/160-ml flask, suggesting an inhibition of the isomerization step of the biohydrogenation. Low pH decreased the ratio of trans-10:trans-11 intermediates. With initial amounts of linoleic acid increasing from 100 to 300 mg, the percentage of linoleic acid disappearance declined, but the amount that disappeared increased, without modification of the trans-10:trans-11 ratio, suggesting a maximal capacity of isomerization rather than an inhibition. Moreover, increasing initial linoleic acid resulted in high amounts of trans-C18:1 and an increase of C18:0 that was a linear function of time, suggesting a maximal capacity for the second reduction step of biohydrogenation. High amounts of initial linolenic acid did not affect the amounts of CLA, trans-C18:1, or the ratio trans-10:trans-11. Based on these experiments, a ruminal pH near neutrality with high amount of dietary linoleic acid should modulate the reactions of biohydrogenation in a way that supports CLA and trans-11C18:1 in the rumen.
Asunto(s)
Ácidos Linoleicos/análisis , Ácidos Linoleicos/metabolismo , Ácidos Linolénicos/análisis , Ácidos Linolénicos/metabolismo , Rumen/metabolismo , Animales , Bovinos , Concentración de Iones de Hidrógeno , Hidrogenación , Isomerismo , Cinética , Ácidos Linoleicos Conjugados/análisis , Ácidos Linoleicos Conjugados/metabolismoRESUMEN
The objective of this study was to investigate the relationship between the concentrations of the different ketone bodies in milk and blood and to evaluate these concentrations for the detection of subclinical ketosis. A total of 60 multiparous cows were used. Concentrations of acetone, acetoacetate, and beta-hydroxybutyrate were analyzed quantitatively in blood and milk, and the Ketolac strip test was used for semiquantitative determination of beta-hydroxybutyrate in milk. Cows were defined subclinically ketotic when their concentration of blood beta-hydroxybutyrate was over 1200 micromol/L. High correlation coefficients were observed between blood acetone and blood acetoacetate, and between blood and milk acetone. On the contrary, concentrations of milk and blood beta-hydroxybutyrate were poorly correlated with the other concentrations of ketone bodies. The Ketolac strip test overestimated the concentrations of beta-hydroxybutyrate in milk. For the detection of subclinical ketosis, the best sensitivity-specificity combination was obtained with the determination of acetoacetate in blood or milk, with threshold concentrations of 125 and 50 micromol/L, respectively. Determination of beta-hydroxybutyrate in the milk via an enzymatic analysis or via the Ketolac strip test provided valuable results, with threshold concentrations of 70 and 100 micromol/L, respectively. The simplicity of use of the Ketolac strip test makes it a valuable way to investigate subclinical ketosis.
Asunto(s)
Enfermedades de los Bovinos/diagnóstico , Bovinos/fisiología , Cuerpos Cetónicos/análisis , Cetosis/veterinaria , Leche/química , Ácido 3-Hidroxibutírico/análisis , Acetoacetatos/análisis , Acetona/análisis , Animales , Femenino , Cetosis/diagnóstico , Tiras Reactivas , Sensibilidad y Especificidad , Valores Limites del UmbralRESUMEN
Four dairy cows fitted with a duodenal cannula were used in a 4 x 4 Latin square design to investigate the effects of daily duodenal infusion of 500 g of fatty acids (containing mainly C16:0, C18:0, or cis-C18:1) on fecal concentrations of fatty acids, fatty acid profiles of milk fat, and solid fat content of butter. Fecal concentrations of C16:0 and especially of C18:0 were increased by duodenal infusion. Infusion with C16:0 increased the proportion of C16:0 in milk fat and delayed softening of butter when the temperature rose. Infusion with C18:0 resulted only in a slight increase of C18:0 proportion in milk fat and did not significantly affect solid fat in butter between -10 and 30 degrees C. With the infusion of cis-C18:1, the proportion of cis-C18:1 in milk fat was more than twice that of control, to the detriment of C16:0. Butter contained low proportion of solid fat, even at low temperatures. Increasing C16:0 or cis-C18:1 in milk fatty acid via duodenal infusion can be used to study their specific effects on butter characteristics, but, because of a low transfer from infusion to milk, this method is less efficient with C18:0.
Asunto(s)
Mantequilla/análisis , Duodeno , Leche/química , Ácido Oléico/administración & dosificación , Ácido Palmítico/administración & dosificación , Ácidos Esteáricos/administración & dosificación , Animales , Bovinos , Digestión , Ácidos Grasos/análisis , Heces/química , Femenino , Calor , Lípidos/análisis , Ácido Oléico/análisis , Ácido Palmítico/análisis , Ácidos Esteáricos/análisisRESUMEN
Twenty multiparous Holstein cows were used in a 16-wk trial. A block of 10 cows received a control diet, based on corn silage, and the other block of 10 cows successively received four diets with 1) an extruded blend of canola meal and canola seeds, 2) canola meal and whole canola seeds, 3) canola meal and ground canola seeds, or 4) canola meal and calcium salts of canola oil fatty acids. Canola fat represented about 2% of dietary dry matter. Compared to control cows, treated cows had similar dry matter intake, milk production, and daily milk output of true protein or fat. Protein contents of milk was decreased by all treatments, with a lower effect of extruded or whole canola seeds. Milk fat contents was lowered by all treatments, extruded seeds and calcium salts resulting in most important effects. All treatments lowered the percentage of fatty acids with 12 to 16 carbons in milk fat, increased C18:0 and cis-C18:1 percentages, and the proportion of liquid fat in butter between 0 and 12 degrees C. Calcium salts and, to a lesser extent extruded seeds, resulted in most important improvements of milk fatty acid profile and butter softness, whereas whole seeds had low effects.
Asunto(s)
Mantequilla , Bovinos/fisiología , Grasas Insaturadas en la Dieta/administración & dosificación , Ácidos Grasos Monoinsaturados/administración & dosificación , Ácidos Grasos/administración & dosificación , Leche/química , Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Mantequilla/análisis , Ácidos Grasos/análisis , Ácidos Grasos Monoinsaturados/química , Femenino , Calor , Lactancia , Lípidos/análisis , Proteínas de la Leche/análisis , Aceite de Brassica napusRESUMEN
The effect of dietary lipids on the fatty acid (FA) profile of cows' milk fat is mainly dependent on digestive processes and mammary gland uptake and metabolism of FA. The objective of this study was to determine the separate effects of high arterial concentrations of 16:0, 18:0 and cis-18:1(n-9) on uptake, synthesis and 18:0 desaturation rate in the mammary gland of lactating dairy cows, via arterio-venous differences and mammary gland balance of FA. In a 4 x 4 Latin square, four lactating Holstein cows with cannula in the proximal duodenum were infused duodenally with a mixture providing daily 0 (C treatment) or 500 g FA with mainly 16:0 (P treatment), 18:0 (S treatment) or cis-18:1(n-9) (O treatment). Significantly higher arterial concentrations of infused FA in arterial plasma nonesterified FA and triglycerides (NETGFA) were observed with P and O treatments, but the effect of the S treatment was much lower. Arterio-venous differences of NETGFA increased with arterial concentrations. The number of synthesized FA in the mammary gland was not significantly affected by duodenal infusion of FA. Mean chain length was significantly reduced by P and O treatments, suggesting an effect of mammary gland uptake of long-chain FA on the termination process of mammary gland synthesis of FA. Across all treatments, 4:0 mammary gland balance increased linearly (r = 0.67, P = 0.004) with mammary gland FA uptake. Mammary gland desaturation of 18:0 to cis-18:1(n-9) averaged 52% and was not significantly affected by treatments, but was reduced by trans-18:1 mammary gland uptake. Uptake, synthesis and desaturation of FA by the mammary gland of dairy cows are affected by arterial concentrations of 16:0, 18:0 and cis-18:1(n-9).
Asunto(s)
Ácidos Grasos/administración & dosificación , Lactancia , Glándulas Mamarias Animales/metabolismo , Leche/metabolismo , Ácido Palmítico/administración & dosificación , Ácidos Esteáricos/administración & dosificación , Animales , Bovinos , Dieta , Duodeno , Ácidos Grasos/biosíntesis , Ácidos Grasos/sangre , Ácidos Grasos no Esterificados/sangre , Femenino , Glándulas Mamarias Animales/irrigación sanguínea , Ácidos Esteáricos/metabolismo , Triglicéridos/sangreRESUMEN
Dairy cows fitted with ruminal, duodenal and ileal cannulas were utilized to investigate the effects of feeding with Ca soaps (CaS) of palm fatty acids (FA) and rapeseed FA. Diets compared were control diet based on maize silage and concentrate, and two diets with 40 g CaS of palm oil FA or rapeseed oil FA/kg diet, replacing part of the concentrates of the control diet. Total digestibilities of dry matter, fibre and fat, and ruminal fermentation were not significantly altered by giving CaS; the extent of ruminal biohydrogenation of total unsaturated C18 FA was significantly reduced by both CaS diets. Apparent intestinal digestibility of FA was not different among diets, although the amount of FA absorbed with the CaS diets was twice that with the control diet. No difference among diets was observed for milk production, or fat and protein contents. Giving CaS diets decreased the proportions of 4:0 to 14:0 FA in milk fat, and increased cis-18:1n-9, compared with control diet. The rapeseed diet lowered the content of 16:0, and increased the contents of 18:0 and trans-18:1n-7. CaS diets did not result in a marked increase of polyunsaturated FA content in milk fat. Butter from cows fed on the CaS diets contained more liquid fat at 6 and 14 degrees C than butter from the cows fed on the control diet. Incorporating CaS, particularly those from rapeseed, in dairy cows' diets increased C18 FA in milk and improved butter spreadability.
Asunto(s)
Mantequilla/análisis , Calcio de la Dieta/farmacología , Bovinos/metabolismo , Dieta/veterinaria , Digestión/efectos de los fármacos , Ácidos Grasos Insaturados/farmacología , Leche/química , Amoníaco/análisis , Amoníaco/metabolismo , Animales , Calcio de la Dieta/administración & dosificación , Calcio de la Dieta/metabolismo , Bovinos/fisiología , Digestión/fisiología , Duodeno/química , Duodeno/metabolismo , Ácidos Grasos Insaturados/administración & dosificación , Ácidos Grasos Insaturados/metabolismo , Ácidos Grasos Volátiles/análisis , Ácidos Grasos Volátiles/metabolismo , Femenino , Fermentación , Concentración de Iones de Hidrógeno , Íleon/química , Íleon/metabolismo , Lactancia/fisiología , Leche/metabolismo , Rumen/química , Rumen/metabolismo , Rumen/fisiologíaRESUMEN
Four lactating cows fitted with ruminal, duodenal, and ileal cannulas were used to determine the effects of extrusion of whole horse beans on ruminal CP degradation and absorption of AA from the small intestine. Diets contained 15% CP, of which 46% was provided by the raw or extruded whole horse beans. Spot samples of duodenal and ileal digesta were collected during a 72-h period. The markers Cr-EDTA, YbCl3, and purines were used for liquid, particulate, and bacteria, respectively. Extruded whole horse beans increased AA flow to the duodenum and disappearance in the small intestine. Diets containing extruded whole horse beans increased availability of total essential AA in the small intestine compared with diets containing raw whole horse beans.
Asunto(s)
Aminoácidos/metabolismo , Alimentación Animal , Bovinos/fisiología , Proteínas en la Dieta/metabolismo , Digestión , Fabaceae , Plantas Medicinales , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Proteínas en la Dieta/administración & dosificación , Femenino , Absorción Intestinal , Rumen/metabolismoRESUMEN
Four cannulated, lactating cows were used to determine the effects of extruding white lupin seeds (WLS) on intraruminal CP degradation and absorption of AA from the small intestine. Raw lupin seeds (RWLS) and extruded lupin seeds (EWLS) were fed in diets containing 15.5% CP and composed of 22.4% WLS, 55.9% corn silage, 10.1% maize grain, and 10.6% Italian ryegrass on a DM basis; WLS provided approximately 50% of the total dietary CP. Spot samples of digesta were collected from the proximal duodenum and terminal ileum during 72 h. Chromium EDTA, YbCl3, and purines were used as liquid, particulate, and bacterial markers, respectively. Because of the extensive ruminal degradation of dietary N with the RWLS diet compared with the EWLS diet (64.2 vs 38.8%), less total AA flowed to the duodenum (1,748 vs 2,347 g/d), and because of the decrease of availability (67.2 vs 79.0% of entering), absorption from the small intestine was lowest with this diet (1,175 vs 1,855 g/d). The apparent digestion of individual AA in the small intestine (percentage entering) ranged from 46.5 to 88.4 when cows consumed RWLS and from 58.9 to 94.4 when they were fed the EWLS diet. Among the diets, apparent absorption of histidine, lysine, glycine, and serine were higher than those of other AA; also, valine, alanine, and proline showed intestinal absorptions that were somewhat lower than those of the remainder of the AA.
Asunto(s)
Aminoácidos/farmacocinética , Alimentación Animal , Bovinos/fisiología , Proteínas en la Dieta/metabolismo , Digestión , Alimentación Animal/análisis , Animales , Disponibilidad Biológica , Fabaceae , Femenino , Manipulación de Alimentos , Tránsito Gastrointestinal , Calor , Absorción Intestinal , Mucosa Intestinal/metabolismo , Intestinos/fisiología , Plantas Medicinales , Rumen/fisiología , SemillasRESUMEN
Experiments were conducted to determine the effects of supplementing chopped ryegrass hay with 0, 5, 10, or 20% fat coated with ruminal bypass protein (Prolip on ruminal digestion by cows and nutrient digestibility by sheep. Ruminal disappearances of DM, ADF, and NDF were measured by the in sacco technique using a cannulated cow. Degradation of DM, ADF, and NDF was not affected by protected fat supplementation at the 5, 10, or 20% level during the first 16 h in the rumen. In addition, supplementation with Prolip did not influence in vitro fermentation characteristics such as pH and VFA patterns. A digestibility experiment with six mature sheep in a switch-back design showed no decrease in feed intake or other undesired effects between unsupplemented (basal) and Prolip-supplemented diets. Whatever the diet fed to sheep, fiber digestibility was similar; however, DM and nitrogen digestibilities were significantly higher relative to the basal diet when ryegrass hay was substituted at 20% (64.2 vs 59.0% and 74.1 vs 71.1%, respectively). Ether extract digestion was enhanced by increasing level of Prolip; the corresponding values were 48.0, 64.9, 74.6, and 84.6% at 0, 5, 10, or 20% Prolip. Likewise, Prolip supplementation at the 10 or 20% level augmented digestibility of energy (62.7 or 65.9 vs 59.2%). Nitrogen and energy retentions were improved due to the decreases in fecal and(or) urinary excretion(s).
Asunto(s)
Alimentación Animal , Grasas de la Dieta/metabolismo , Digestión , Rumen/fisiología , Ovinos/fisiología , Animales , Grasas de la Dieta/administración & dosificación , Fibras de la Dieta/metabolismo , Proteínas en la Dieta/administración & dosificación , Fermentación , Alimentos Fortificados , Rumen/microbiologíaRESUMEN
Four mature lactating Holstein cows fitted with permanent ruminal, duodenal and ileal cannulae were used to study the effect of extrusion at 195 degrees C of beans (Vicia faba cv Castel) on organic matter (OM), nitrogen (N) and starch degradation in the rumen and their flow to and absorption from the small intestine. The test protein sources, raw beans (RB) and extruded beans (EB), provided about 45% of the dietary protein. The diets were composed of 23.1% beans, 56.2% corn silage, 10.1% corn grain and 10.7% Italian rye-grass hay on a DM basis; the diets were isocaloric (4.5 Mcal/kg of DM) and isonitrogenous (14% of DM). Cr-EDTA, YbCl3 and purines were used as liquid, particulate and bacterial markers respectively. Extruding the beans did not influence intraruminal pH (6.6), ammonia-N (99 mg/l) and volatile fatty acids (97 mM/l) concentrations. Apparent digestibility in total tract of energy, OM, N and starch were not affected with inclusion of EB instead of RB, the corresponding mean values were: 66, 68, 64 and 95% of intake. Apparent ruminal digestion of OM, starch and N in the forestomach were 39, 58 and 52% for diets containing RB and 38, 72 and 45% for EB diets; efficiency of bacterial protein synthesis (gN/kg organic matter truly digested in the stomach) was higher for EN diets compared with RN diets (25 vs 22). Eating diets including EB increased non-ammonia-N, bacterial-N and dietary-N flows (g/d) to the duodenum compared with diets containing RB: 409 vs 366, 216 vs 194 and 193 vs 172 respectively; while starch flow was reduced (1.5 vs 2.2 kg/d) and OM flow was unchanged (9.8 kg/d). Apparent digestion from the small intestine (g/d) of nonammonia-N, bacterial-N and dietary-N were higher for EB diets: 268 vs 229, 181 vs 160 and 87 vs 69 respectively; meanwhile, starch digestion decreased (975 vs 1,612). The PDIA, PDIN and PDIE contents (g/kg of DM) of the RB were 33, 175 and 127 respectively; the corresponding values after extrusion were: 58, 147 and 178.
Asunto(s)
Alimentación Animal , Bovinos/fisiología , Fabaceae , Intestino Delgado/metabolismo , Lactancia , Nitrógeno/metabolismo , Plantas Medicinales , Almidón/metabolismo , Animales , Digestión , Femenino , Calor , Absorción IntestinalRESUMEN
Four lactating cows fitted with permanent ruminal, duodenal and ileal cannulae were used to study the effect of extrusion of whole lupin seeds at 195 degrees C (Lupinus albus cv Lublanc) on organic matter (OM) and nitrogen (N) degradation in the rumen and their flow to and absorption from the small intestine. Raw whole lupin seeds (RWLS) and extruded whole lupin seeds (EWLS) were fed in diets containing 15.5% crude protein and composed of 22.6% whole lupin seeds, 56.5% corn silage, 10.2% corn grain and 10.7% Italian ray-grass on a DM basis, supplemented with vitamins and minerals. Chromium ethylenediaminotetraacetic (Cr-EDTA) and ytterbium chloride (YbCl3) were used as liquid and particulate markers respectively, while purines and 15N ammonium sulfate were utilized as bacterial markers. Cows fed EWLS had a similar ruminal ammonia N and volatile fatty acid concentrations and efficiency of bacterial protein synthesis compared to those fed the RWLS diet. Total tract OM and N digestion were not affected by inclusion of EWLS instead of RWLS; the corresponding mean values were 70 and 71%. Apparent degradation of OM and N in the rumen were 44 and 64% for diets containing RWLS, and 40 and 39% for EWLS diets. Feeding diets including EWLS both increased non ammonia N and dietary N flow to the duodenum compared with diets containing RWLS (472 vs 357 g/d) and (263 vs 153 g/d) respectively. Absorption from the small intestine (g/d and % entering) of dietary N was higher for EWLS diets (146 vs 62 g/d; 34 vs 15%). The PDIA, PDIE and PDIN contents (g/kg of DM) of RWLS were 18, 94 and 245 respectively; the corresponding values after extrusion were 145, 220 and 220.
Asunto(s)
Alimentación Animal , Bovinos/fisiología , Proteínas en la Dieta/metabolismo , Digestión , Absorción Intestinal , Proteínas de Plantas/metabolismo , Rumen/metabolismo , Animales , Femenino , Fermentación , Nitrógeno/metabolismo , Semillas , SolubilidadRESUMEN
Four rumen and proximal duodenum fistulated non-lactating Holstein cows were used to determine the effect of extrusion at 120 degrees C of whole horse beans (Vicia faba cv Talo) on in vitro nitrogen (N) solubility and in situ degradation of dry matter (DM) and crude protein (CP) in the rumen and intestine. Cows were fed a ration of 30% whole horse beans (WHB) and 70% Italian rye-grass hay. The degradation of DM and CP was estimated using nylon bags suspended in the rumen for 2, 4, 7, 16, 24 and 48 h; the effective ruminal degradability of DM and CP was evaluated assuming a ruminal outflow rate of 0.06/h. Bags incubated in the rumen for 16 h were introduced into the small intestine through the duodenal cannula and subsequently recovered in the feces. Extrusion of WHB reduced N-solubility in buffer solution (21.1 vs 74.9%). Processing diminished the effective rumen degradability of DM (74.6 vs 80.4%) and CP (70.2 vs 89.2%). Meanwhile, the amounts of DM and CP digested in the intestine increased: 9.6 vs 1.4% and 25.2 vs 3.0% respectively. Therefore, feeds containing extruded WHB increase the availability of dietary proteins in the intestine compared with diets containing raw WHB.
Asunto(s)
Alimentación Animal , Bovinos/fisiología , Proteínas en la Dieta/metabolismo , Digestión , Fabaceae , Mucosa Intestinal/metabolismo , Plantas Medicinales , Rumen/metabolismo , Animales , Disponibilidad Biológica , SolubilidadRESUMEN
The effect of whole lupin seeds (Lupinus albus cv Lublanc) at 120, 150 and 195 degrees C on in situ nitrogen degradability (Dg.N) was measured by the nylon bag technique using fistulated non-lactating Holstein cows. The N degradation was evaluated in nylon bags suspended in the rumen; heating the seeds at 120, 150 and 195 degrees C decreased the Dg.N value: 83.9, 72.9 and 53.0 respectively vs 95.3% (rumen outflow rate of 0.06/h). To estimate the total N disappearing in the digestive tract, bags were incubated in the rumen for 16 h, then in a pepsin bath for 2 h and then introduced into the duodenum for subsequently recovery in feces. The whole tract degradability of N was always high, approximately 98.3%. The amounts of N which disappeared in the intestine increased from 3.1 (untreated seeds) to 15.1, 26.3 and 44.7% as the temperature rose to 120, 150 and 195 degrees C respectively. The PDIN and PDIE contents (g/kg of DM) of the raw whole lupin seeds were 224 and 84 respectively; extrusion elevated these values by 10-32% for PDIN and 57-194% for PDIE. The augmentation in the supply of dietary proteins to the postruminal parts as a result of extrusion could rapidly benefit high yielding cows.