RESUMEN
The role of brain cholesterol in Alzheimer's disease (AD) is currently a matter of debate. Experimental evidence suggests that reducing circulating and brain cholesterol protects against AD, however recent data indicate that low membrane cholesterol results in neurode-generation and that the cholesterol synthesis catalyst seladin-1 is down-regulated in AD-affected brain regions. We previously reported a significant correlation between resistance to amyloid toxicity and content of membrane cholesterol in differing cultured cell types. Here we provide evidence that Abeta42 pre-fibrillar aggregates accumulate more slowly and in reduced amount at the plasma membrane of human SH-SY5Y neuroblastoma cells overexpressing seladin-1 or treated with PEG-cholesterol than at the membrane of control cells. The accumulation was significantly increased in cholesterol-depleted cells following treatment with the specific seladin-1 inhibitor 5,22E-cholestadien-3-ol or with methyl-beta-cyclodextrin. The resistance to amyloid toxicity and the early cytosolic Ca2+ rise following exposure to Abeta42 aggregates were increased and prevented, respectively, by increasing membrane cholesterol whereas the opposite effects were found in cholesterol-depleted cells. These results suggest that seladin-1-dependent cholesterol synthesis reduces membrane-aggregate interaction and cell damage associated to amyloid-induced imbalance of cytosolic Ca2+. Our findings extend recently reported data indicating that seladin-1 overexpression directly enhances the resistance to Abeta toxicity featuring seladin-1/DHCR 24 as a possible new susceptibility gene for sporadic AD.
Asunto(s)
Péptidos beta-Amiloides/toxicidad , Colesterol/biosíntesis , Membranas/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Neuroblastoma/metabolismo , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH/metabolismo , Enfermedad de Alzheimer/genética , Enfermedad de Alzheimer/metabolismo , Membrana Celular/metabolismo , Células Cultivadas , Estructuras Celulares/metabolismo , Humanos , Neuroblastoma/patologíaRESUMEN
An acylphosphatase (AcPase) overexpression study was carried out on SH-SY5Y neuroblastoma cells, using a green fluorescent fusion protein (AcP-GFP), with GFP acting as a reporter protein. The cellular proliferation rate was significantly reduced by overexpression of AcPase by a factor of ten. In contrast, clones transfected with two inactive AcPase mutants showed a growth rate comparable to control cells. This suggests that AcPase catalyzes the proliferative down-regulation. AcPase-overexpressing clones showed a physiological mortality rate as assessed by an MTT reduction test and by evaluation of necrotic markers. DNA fragmentation analysis and assays of caspase-3 and poly (ADP-ribose) polymerase (PARP)-active fragments showed no evidence of any apoptotic pattern. AcPase overexpression led to a marked increase in PARP activity as well as Bcl-2 content; these are commonly up-regulated during differentiative processes in neuronal cells. In fact, the typical differentiation marker, growth-associated-protein 43, was significantly up-regulated. Microscopic observations also showed a clear increase in the differentiative phenotype in AcPase-overexpressing cells. Our results clearly show that AcPase plays a primary causative role in neuronal differentiation.
Asunto(s)
Ácido Anhídrido Hidrolasas/genética , Diferenciación Celular/fisiología , Neuroblastoma/metabolismo , Ácido Anhídrido Hidrolasas/biosíntesis , Apoptosis/fisiología , Biomarcadores , División Celular , Genes Reporteros , Oxidación-Reducción , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , AcilfosfatasaRESUMEN
BACKGROUND: It is well documented that exercise reduces the risk of thromboembolic disease, possibly by increasing the plasma concentration of anticoagulant-antithrombotic compounds. OBJECTIVES: As plasma glycosaminoglycans (GAGs) play a role in the anticoagulant-antithrombotic potential of plasma, to examine the concentration and profile of these compounds in well trained, long distance runners and sedentary subjects. METHODS: Plasma GAGs were measured in 10 male, long distance runners and 10 sedentary counterparts before and after ergometric tests. GAGs were extracted, purified, and identified by electrophoretic and enzymatic methods, and measured as hexosamine. RESULTS: Plasma GAGs found in sedentary subjects were slow migrating heparan sulphates I and II, keratan sulphate I, and chondroitin 4-6-sulphate. Those found in trained athletes were slow migrating heparan sulphate I, chondroitin 4-6-sulphate (or keratan sulphate I), and fast migrating heparan sulphate. Total plasma concentrations of GAGs were higher in athletes than in sedentary subjects at rest. In sedentary subjects, plasma GAGs did not change after cycle ergometric exercise at 80% of their anaerobic threshold. However, the appearance of a novel band of heparan sulphate migrating faster than fast migrating heparan sulphate was observed in athletes after exercise. CONCLUSIONS: Exercise changes the amount and profile of plasma GAGs; these changes may play a role in protecting subjects who practise aerobic sports against developing cardiovascular disease.
Asunto(s)
Glicosaminoglicanos/sangre , Carrera/fisiología , Adulto , Antropometría , Sulfatos de Condroitina/sangre , Prueba de Esfuerzo/métodos , Heparitina Sulfato/sangre , Humanos , Sulfato de Queratano/sangre , Estilo de Vida , MasculinoRESUMEN
To identify early adaptive processes of cardiac remodeling (CR) in response to volume overload, we investigated the molecular events that may link intracellular Ca(2+) homeostasis alterations and cardiomyocyte apoptosis. In swine heart subjected to aorto-cava shunt for 6, 12, 24, 48 and 96 h sarcoplasmic reticulum (SR) Ca(2+) pump activity was reduced until 48 h (-30%), but a recovery of control values was found at 96 h. The decrease in SR Ca(2+)-ATPase (SERCA2a) expression at 48 h, was more marked (-60%) and not relieved by a subsequent recovery, while phospholamban (PLB) concentration and phosphorylation were unchanged at all the considered times. Conversely, acylphosphatase activity and expression significantly increased from 48 to 96 h (+40%). Bcl-2 expression increased significantly from 6 to 24 h, but at 48 h, returned to control values. At 48 h, microscopic observations showed that overloaded myocardium underwent substantial damage and apoptotic cell death in concomitance with an enhanced Fas/Fas-L expression. At 96 h, apoptosis appeared attenuated, while Fas/Fas-L expression was still higher than control values and cardiomyocyte hypertrophy became to develop. These data suggest that in our experimental model, acylphosphatase could be involved in the recovery of SERCA2a activity, while cardiomyocyte apoptosis might be triggered by a decline in Bcl-2 expression and a concomitant activation of Fas.
Asunto(s)
Ácido Anhídrido Hidrolasas/fisiología , Cardiomiopatías/metabolismo , Remodelación Ventricular/fisiología , Animales , Apoptosis , Calcio/metabolismo , Proteínas de Unión al Calcio/metabolismo , ATPasas Transportadoras de Calcio/biosíntesis , ATPasas Transportadoras de Calcio/metabolismo , Volumen Cardíaco , Cardiomiopatías/patología , Electrocardiografía , Proteína Ligando Fas , Hemodinámica , Glicoproteínas de Membrana/biosíntesis , Proteínas Proto-Oncogénicas/biosíntesis , ATPasas Transportadoras de Calcio del Retículo Sarcoplásmico , Porcinos , Factores de Tiempo , Receptor fas/biosíntesis , AcilfosfatasaRESUMEN
Ca2+ transport by sarco/endoplasmic reticulum, tightly coupled with the enzymatic activity of Ca2+ -dependent ATPase, controls the cell cycle through the regulation of genes operating in the critical G, to S checkpoint. Experimental studies demonstrated that acylphosphatase actively hydrolyses the phosphorylated intermediate of sarco/endoplasmic reticulum calcium ATPase (SERCA) and therefore enhances the activity of Ca2+ pump. In this study we found that SH-SY5Y neuroblastoma cell division was blocked by entry into a quiescent G0-like state by thapsigargin, a high specific SERCA inhibitor, highlighting the regulatory role of SERCA in cell cycle progression. Addition of physiological amounts of acylphosphatase to SY5Y membranes resulted in a significant increase in the rate of ATP hydrolysis of SERCA. In synchronized cells a concomitant variation of the level of acylphosphatase isoenzymes opposite to that of intracellular free calcium during the G1 and S phases occurs. Particularly, during G1 phase progression the isoenzymes content declined steadily and hit the lowest level after 6 h from G0 to G1 transition with a concomitant significant increase of calcium levels. No changes in free calcium and acylphosphatase levels upon thapsigargin inhibition were observed. Moreover, a specific binding between acylphosphatase and SERCA was demonstrated. No significant change in SERCA-2 expression was found. These findings suggest that the hydrolytic activity of acylphosphatase increase the turnover of the phosphoenzyme intermediate with the consequences of an enhanced efficiency of calcium transport across endoplasmic reticulum and a subsequent decrease in cytoplasmic calcium levels. A hypothesis about the modulation of SERCA activity by acylphosphatase during cell cycle in SY5Y cells in discussed.
Asunto(s)
Ácido Anhídrido Hidrolasas/metabolismo , ATPasas Transportadoras de Calcio/metabolismo , Calcio/metabolismo , Ácido Anhídrido Hidrolasas/genética , Sustitución de Aminoácidos , Ciclo Celular/fisiología , Medio de Cultivo Libre de Suero , Relación Dosis-Respuesta a Droga , Inhibidores Enzimáticos/farmacología , Citometría de Flujo , Humanos , Neuroblastoma , Pruebas de Precipitina , ATPasas Transportadoras de Calcio del Retículo Sarcoplásmico , Tapsigargina/farmacología , Células Tumorales Cultivadas , AcilfosfatasaRESUMEN
Amyotrophic lateral sclerosis (ALS) is a progressive fatal disorder, which results from the degeneration of motor neurons in the brain and spinal cord. Approximately 20% of the inherited autosomal dominant cases are due to mutations within the gene coding for Cu/Zn superoxide dismutase 1 (SOD1), a cytosolic homodimeric enzyme that catalyzes the dismutation of toxic superoxide anion. We investigated the presence of SOD1 gene mutations and activity alterations in two unrelated families of ALS patients from Elba, an island of central Italy. No mutation in SOD1 exon 1 to 5 and no activity alteration were observed in all members of the two analyzed ALS families (FALS). These data show an apparent heterogeneous distribution of ALS patients with SOD1 gene mutations among different populations and suggest that another genetic locus could be involved in the disease.
Asunto(s)
Esclerosis Amiotrófica Lateral/genética , Mutación/genética , Superóxido Dismutasa/genética , Análisis Mutacional de ADN/estadística & datos numéricos , Exones/genética , Femenino , Humanos , Italia , Masculino , Persona de Mediana Edad , Linaje , Superóxido Dismutasa-1RESUMEN
Intracellular levels of glutathione (GSH), glutathione disulphide (GSSG), glutamic acid and gamma-glutamyl cysteine synthetase (gamma-GCS) were measured in lymphoblast lines from patients with familial and sporadic Alzheimer's disease (AD) and from age-matched controls. Lymphoblasts carrying presenilins (PS) and amyloid precursor protein (APP) genes mutations showed significantly decreased GSH content with respect to controls. Levels of GSSG and glutamic acid, as well as the activity of gamma-GCS were not significantly different in lymphoblasts carrying genes mutations as compared with control cells. These results indicate that even peripheral cells not involved in the neurodegenerative process of AD show altered GSH content when carrying PS and APP genes mutations. The provided data appear to be in accordance with the known alteration of GSH levels in central nervous system and strengthen the hypothesis of oxidative stress as an important, possibly crucial mechanism in the pathogenesis of AD.
Asunto(s)
Enfermedad de Alzheimer/sangre , Enfermedad de Alzheimer/genética , Glutatión/metabolismo , Linfocitos/metabolismo , Precursor de Proteína beta-Amiloide/genética , Glutamato-Cisteína Ligasa/metabolismo , Ácido Glutámico/metabolismo , Disulfuro de Glutatión/metabolismo , Humanos , Activación de Linfocitos , Proteínas de la Membrana/genética , Oxidación-Reducción , Presenilina-1RESUMEN
In cardiac and skeletal muscle Ca2+ translocation from cytoplasm into sarcoplasmic reticulum (SR) is accomplished by different Ca2+-ATPases whose functioning involves the formation and decomposition of an acylphosphorylated phosphoenzyme intermediate (EP). In this study we found that acylphosphatase, an enzyme well represented in muscular tissues and which actively hydrolyzes EP, had different effects on heart (SERCA2a) and fast twitch skeletal muscle SR Ca2+-ATPase (SERCA1). With physiological acylphosphatase concentrations SERCA2a exhibited a parallel increase in the rates of both ATP hydrolysis and Ca2+ transport; in contrast, SERCA1 appeared to be uncoupled since the stimulation of ATP hydrolysis matched an inhibition of Ca2+ pump. These different effects probably depend on phospholamban, which is associated with SERCA2a but not SERCA1. Consistent with this view, the present study suggests that acylphosphatase-induced stimulation of SERCA2a, in addition to an enhanced EP hydrolysis, may be due to a displacement of phospholamban, thus to a removal of its inhibitory effect.
Asunto(s)
Ácido Anhídrido Hidrolasas/farmacología , ATPasas Transportadoras de Calcio/efectos de los fármacos , Músculo Esquelético/efectos de los fármacos , Miocardio/metabolismo , Retículo Sarcoplasmático/efectos de los fármacos , Ácido Anhídrido Hidrolasas/genética , Ácido Anhídrido Hidrolasas/metabolismo , Adenosina Trifosfato/metabolismo , Animales , Calcio/metabolismo , Proteínas de Unión al Calcio/fisiología , ATPasas Transportadoras de Calcio/metabolismo , Bovinos , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Relación Dosis-Respuesta a Droga , Corazón/efectos de los fármacos , Músculo Esquelético/metabolismo , Mutación , Orgánulos/efectos de los fármacos , Orgánulos/metabolismo , Fosfatos/metabolismo , Fosforilación/efectos de los fármacos , Pruebas de Precipitina , Conejos , Retículo Sarcoplasmático/metabolismo , AcilfosfatasaRESUMEN
An open reading frame encoding a putative acylphosphatase was found in Drosophila melanogaster. The corresponding gene product shows 40% identity and 22 additional amino acid residues at the C-terminus as compared to muscle- and common-type human acylphosphatases. Moreover, all the residues involved in the catalytic mechanism of vertebrate enzymes are conserved in the D. melanogaster acylphosphatase. The D. melanogaster protein and a deletion mutant, similar in length to vertebrate acylphosphatases, were produced by cloning the corresponding cDNA in Escherichia coli. The wild-type enzyme is a protein with a well-established three-dimensional fold and a markedly reduced conformational stability as compared to vertebrate isoenzymes. The specific activity of the enzyme is significantly lower than that found in vertebrate enzymes though the substrate binding capability is basically unaltered. The deletion of 22 residues does not cause a significant change in k(cat), while affecting the apparent binding parameters. This work suggests that the genes encoding the vertebrate enzymes originate from an ancestor gene by duplication and subsequent evolution.
Asunto(s)
Ácido Anhídrido Hidrolasas/genética , Drosophila melanogaster/enzimología , Evolución Molecular , Isoenzimas/genética , Filogenia , Ácido Anhídrido Hidrolasas/química , Ácido Anhídrido Hidrolasas/metabolismo , Secuencia de Aminoácidos , Animales , Clonación Molecular , Secuencia Conservada , Drosophila melanogaster/genética , Escherichia coli , Humanos , Isoenzimas/química , Isoenzimas/metabolismo , Cinética , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Sistemas de Lectura Abierta , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Vertebrados , AcilfosfatasaRESUMEN
Levels of acylphosphatase isoenzymes and free intracellular calcium have been investigated in cultured SH-SY5Y human neuroblastoma cells under stimulation with all-trans retinoic acid and phorbol-12-myristate-13-acetate. Under these conditions morphological and functional characteristics demonstrated the differentiation of SH-SY5Y cells towards neuronal phenotype. Retinoic acid treatment caused a progressive and synchronous increase of the organ common-type acylphosphatase and of free intracellular calcium but not of the muscle-type acylphosphatase. Phorbol-12-myristate-13-acetate treatment gave rise to a peak of the muscle-type acylphosphatase levels during the early differentiation stage whereas organ common-type isoenzyme and free calcium levels show a pattern similar to that observed in retinoic acid-treated cells. These evidences indicate that the two acylphosphatase isoenzymes play different roles in SH-SY5Y differentiation and that during this process the expression of organ common-type acylphosphatase increases in a synchronous way with intracellular free calcium concentration.
Asunto(s)
Ácido Anhídrido Hidrolasas/efectos de los fármacos , Calcio/metabolismo , Proteínas de Neoplasias/efectos de los fármacos , Neuroblastoma/metabolismo , Ácido Anhídrido Hidrolasas/metabolismo , Carcinógenos/farmacología , Diferenciación Celular/efectos de los fármacos , División Celular/efectos de los fármacos , Humanos , Proteínas de Neoplasias/metabolismo , Neuroblastoma/patología , Ésteres del Forbol/farmacología , Factores de Tiempo , Tretinoina/farmacología , Células Tumorales Cultivadas/efectos de los fármacos , AcilfosfatasaRESUMEN
BACKGROUND: Acylphosphatase (ACP) is a low molecular weight phosphomonohydrolase catalyzing with high specificity the hydrolysis of the carboxyl-phosphate bond present in acylphosphates. The enzyme is thought to regulate metabolic processes in which acylphosphates are involved, such as glycolysis and the production of ribonucleotides. Furthermore the enzyme is capable of hydrolyzing the phospho-aspartyl intermediate formed during the action of membrane pumps such as (Ca2++Mg2+) ATPase. Although the tertiary structure of a muscle ACP has been determined by NMR spectroscopy, little is known about the catalytic mechanism of ACP and further structures might provide an increased understanding. RESULTS: The structure of 'common type' ACP from bovine testis has been determined by X-ray crystallography to a resolution of 1.8 A. The structure has been refined to an R factor of 17.0 % using all data between 15 and 1.8 A. The binding of a sulphate and a chloride ion in the active centre allows a detailed description of this site. The overall protein folds of common type and muscle ACP are similar but their loops have very different conformations. These differences, in part, are probably caused by the binding of the ions in the active site of the common type form. The phosphate-binding loop of ACP shows some remarkable similarities to that of low molecular weight protein tyrosine phosphatase. CONCLUSIONS: The active site of ACP has been located, enabling a reaction mechanism to be suggested in which the phosphate moiety bound to Arg23 acts as a base, abstracting a proton from a nucleophilic water molecule liganded to Asn41. The transition-state intermediate is stabilized by the phosphate-binding loop. We suggest the catalysis to be substrate assisted, which probably explains why this enzyme can only hydrolyze acylphosphates.
Asunto(s)
Ácido Anhídrido Hidrolasas/química , Testículo/enzimología , Animales , Sitios de Unión , Bovinos , Cristalografía por Rayos X , Espectroscopía de Resonancia Magnética , Masculino , Modelos Químicos , Datos de Secuencia Molecular , Unión Proteica , Pliegue de Proteína , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , AcilfosfatasaRESUMEN
Levels of free intracellular calcium have been measured on two cell lines of cultured human fibroblasts carrying the genetic lesions occurring in Duchenne and Becker dystrophies. Both cell lines elicited a markedly higher content of the cation (98 nM and 57 nM, respectively) than control fibroblasts (35 nM). Differences toward controls were statistically significant (p < 0.01). Dystrophic fibroblasts were also found to possess a significantly reduced amount by about 50% of muscular acylphosphatase isoenzyme as compared to normal cells. As acylphosphatase was demonstrated to be involved in the regulation of Ca2+-ATPase activity from different sources, a hypothesis was formulated that could explain the disruption of calcium homeostasis as an effect of the altered acylphosphatase activity.
Asunto(s)
Ácido Anhídrido Hidrolasas/metabolismo , Calcio/metabolismo , Isoenzimas/metabolismo , Distrofias Musculares/metabolismo , Piel/metabolismo , Adolescente , División Celular , Línea Celular , Células Cultivadas , Fibroblastos , Humanos , Masculino , Músculo Esquelético/enzimología , Valores de Referencia , AcilfosfatasaRESUMEN
Ca2+ transport by cardiac sarcoplasmic reticulum is tightly coupled with the enzymatic activity of Ca2+-dependent ATPase, which forms and decomposes an intermediate phosphoenzyme. Heart sarcoplasmic reticulum Ca2+ pump is regulated by cAMP-dependent protein kinase (PKA) phospholamban phosphorylation, which results in a stimulation of the initial rates of Ca2+ transport and Ca2+ ATPase activity. In the present studies we found that acylphosphatase from heart muscle, used at concentrations within the physiological range, actively hydrolyzes the phosphoenzyme of cardiac sarcoplasmic reticulum Ca2+ pump, with an apparent Km on the order of 10(-7) M, suggesting an high affinity of the enzyme for this special substrate. In unphosphorylated vesicles acylphosphatase enhanced the rate of ATP hydrolysis and Ca2+ uptake with a concomitant significant decrease in apparent Km for Ca2+ and ATP. In vesicles whose phospholamban was PKA-phosphorylated, acylphosphatase also stimulated the rate of Ca2+ uptake and ATP hydrolysis but to a lesser extent, and the Km values for Ca2+ and ATP were not significantly different with respect to those found in the absence of acylphosphatase. These findings suggest that acylphosphatase, owing to its hydrolytic effect, accelerates the turnover of the phosphoenzyme intermediate with the consequence of an enhanced activity of Ca2+ pump. It is known that phosphorylation of phospholamban results in an increase of the rate at which the phosphoenzyme is decomposed. Thus, as discussed, a competition between phospholamban and acylphosphatase effect on the phosphoenzyme might be proposed to explain why the stimulation induced by this enzyme is less marked in PKA-phosphorylated than in unphosphorylated heart vesicles.
Asunto(s)
Ácido Anhídrido Hidrolasas/metabolismo , Proteínas de Unión al Calcio/metabolismo , ATPasas Transportadoras de Calcio/metabolismo , Miocardio/enzimología , Retículo Sarcoplasmático/enzimología , Adenosina Trifosfato/metabolismo , Animales , Autorradiografía , Calcio/metabolismo , Bovinos , Activación Enzimática , Hidrólisis , Fosforilación , AcilfosfatasaRESUMEN
Acylphosphatase (AcPase), an enzyme that modulates the activity of Ca(2+)-ATPase by hydrolysing its phosphorylated moiety, has been found to be significantly higher in cultured skin fibroblasts from donors affected by early onset familial Alzheimer's disease (EOFAD) with PS-1 and PS-2 gene mutations. Of the two known isoenzymes of acylphosphatase, only the erythrocyte one accounts for the total increase in activity. No relevant alteration was observed in phosphotyrosine phosphatase activity (PTPase), in Ca(2+)-ATPase and Na+, K(+)-ATPase activities of the same cells as compared to age-matched controls. This finding could suggest a possible explanation for the calcium-dependent biochemical alterations previously described in Alzheimer's disease fibroblasts.
Asunto(s)
Ácido Anhídrido Hidrolasas/metabolismo , Enfermedad de Alzheimer/enzimología , Proteínas de la Membrana/genética , Mutación/fisiología , Anciano , Enfermedad de Alzheimer/genética , ATPasas Transportadoras de Calcio/metabolismo , Citosol/enzimología , Femenino , Fibroblastos/metabolismo , Humanos , Isoenzimas/metabolismo , Masculino , Persona de Mediana Edad , Presenilina-1 , Proteínas Tirosina Fosfatasas/metabolismo , Piel/citología , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , AcilfosfatasaRESUMEN
Acylphosphatase purified from heart muscle actively hydrolyzes the phosphoenzyme intermediate of cardiac sarcoplasmic reticulum Ca(2+)-ATPase. This effect was evident with acylphosphatase concentrations (up to 100 units/mg sarcoplasmic reticulum protein) that fall within the physiological range, and the low value of the apparent Km, on the order of 10(-7)M, suggests a high affinity towards this special substrate. Moreover, acylphosphatase addition to sarcoplasmic reticulum vesicles significantly enhanced the rate of Ca(2+)-dependent ATP hydrolysis. Maximal stimulation, observed with 100 units/mg vesicular protein, resulted in an ATPase activity which was about two folds over basal value. The same acylphosphatase concentration increased at a similar extent the rate of ATP driven Ca2+ influx into sarcoplasmic reticulum vesicles. Taken together these findings lead to suppose that acylphosphatase, owing to its hydrolytic activity, induces an accelerated turnover of the phosphoenzyme intermediate, whence an overall stimulation of heart sarcoplasmic reticulum Ca2+ pump, affecting both ATP hydrolysis and Ca2+ influx.
Asunto(s)
Ácido Anhídrido Hidrolasas/farmacología , ATPasas Transportadoras de Calcio/metabolismo , Calcio/metabolismo , Miocardio/metabolismo , Retículo Sarcoplasmático/metabolismo , Animales , Transporte Biológico/efectos de los fármacos , Bovinos , Hidrólisis/efectos de los fármacos , Miocardio/ultraestructura , AcilfosfatasaRESUMEN
A new method for the determination of haemoglobin concentration in human whole blood has been devised. The assay takes advantage of the lysis of erythrocytes that occurs when blood penetrates the pores of a microporous membrane by a strong capillary effect. The released haemoglobin reacts with the reagents previously dried on the membrane, diffuses through the pores of the membrane and produces a uniform coloured spot whose reflectance at 556 nm can be evaluated at the side to that of application of the sample. In the experimental conditions used by authors, a linear relationship was obtained when the reciprocal reflectance was plotted against the haemoglobin concentration. Data obtained by the reflectometric method were compared with reference values on the same samples from centralized laboratories. Statistical analysis gave a correlation coefficient of 0.985 and only 4% of all haemoglobin determinations were outside the 95% confidence interval. No interference was observed by haematocrit, erythrocyte count, mean corpuscular volume or mean corpuscular haemoglobin concentration and leukocyte count. This method proved to be useful for rapid, accurate and precise assay of total haemoglobin on both capillary and venous blood.
Asunto(s)
Hemoglobinometría/métodos , Hemoglobinas/análisis , Recuento de Células Sanguíneas , Interpretación Estadística de Datos , Índices de Eritrocitos , Estudios de Evaluación como Asunto , Hematócrito , Hemoglobinometría/instrumentación , Hemólisis , Humanos , Membranas Artificiales , Filtros Microporos , Análisis de Regresión , EspectrofotometríaRESUMEN
Single crystals of a 'common-type' acylphosphatase from bovine testis have been grown. Crystals belong to space group C2 and have cell dimensions a = 64.6 A, b = 36.5 A, c = 45.2 A and beta = 104.8 and contain one monomer per asymmetric unit. The crystals diffract better than 2.0 A resolution and are well suited for an X-ray structure determination.
Asunto(s)
Ácido Anhídrido Hidrolasas/química , Cristalografía por Rayos X , Animales , Bovinos , Cristalización , Masculino , Testículo/enzimología , AcilfosfatasaRESUMEN
In vitro experiments demonstrated the ability of acylphosphatase to hydrolyze the phosphorylated intermediate that is formed during the activity of Na+, K(+)- and Ca(2+)-ATPases of mammalian cells membranes. In order to investigate the effect of this enzyme on intracellular cation levels, a synthetic gene for human muscle acylphosphatase has been expressed in E. coli strains BL21 and JM101. Intracellular total steady-state calcium concentration, as measured by isotopic exchange, was significantly higher in transformed cells as compared to controls and the rising was dependent on the level of acylphosphatase expression. Accordingly also free intracellular calcium concentration, as measured by Fura-2 fluorescence, increased in transformed cells. On the other hand, phosphate levels were not affected by the expression of acylphosphatase, while sodium and rubidium levels increase in transformed cells. Intracellular pH resulted to be slightly affected by the expression of acylphosphatase, cytoplasm of transformed JM101 bacteria being more alkaline (pH 7.45) as compared to control cells (pH 7.40). On the basis of these results, it can be suggested that acylphosphatase acts in vivo by regulating the cation transport in E. coli.
Asunto(s)
Ácido Anhídrido Hidrolasas/biosíntesis , Calcio/metabolismo , Escherichia coli/metabolismo , Regulación Enzimológica de la Expresión Génica/genética , Ácido Anhídrido Hidrolasas/genética , Transporte Biológico Activo/genética , ATPasas Transportadoras de Calcio/metabolismo , Escherichia coli/enzimología , Escherichia coli/genética , Fura-2 , Humanos , Concentración de Iones de Hidrógeno , Hidrólisis , Músculos/enzimología , Fosforilación/efectos de los fármacos , Potasio/metabolismo , Sodio/metabolismo , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Espectrometría de Fluorescencia , AcilfosfatasaRESUMEN
Acylphosphatase purified from cardiac muscle actively hydrolyzes the phosphoenzyme intermediate of heart sarcolemma Na+,K(+)-ATPase. This effect occurred with acylphosphatase amounts (up to 800 units/mg membrane protein) that fall within the physiological range and the low value of the apparent Km (0.69 x 10(-7) M) indicates a considerable affinity of the enzyme towards this specific substrate. Acylphosphatase addition to purified sarcolemmal vesicles significantly increased the rate of Na+,K(+)-dependent ATP hydrolysis. Maximal stimulation, observed with 800 units/mg protein, resulted in an ATPase activity which was about 2-fold over basal value. The same acylphosphatase amounts significantly stimulated, in a similar and to an even greater extent, the rate of ATP driven Na+ transport into sarcolemmal vesicles. These findings lead to suppose that an accelerated hydrolysis of the phosphoenzyme may result in an enhanced activity of heart sarcolemmal Na+,K+ pump, therefore suggesting a potential role of acylphosphatase in the control of this active transport system.
Asunto(s)
Ácido Anhídrido Hidrolasas/metabolismo , Miocardio/enzimología , Sarcolema/enzimología , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Adenosina Trifosfato/metabolismo , Adenosina Trifosfato/farmacología , Animales , Transporte Biológico Activo , Bovinos , Hidrólisis , Fosforilación , Potasio/farmacología , Sodio/metabolismo , Sodio/farmacología , Especificidad por Sustrato , AcilfosfatasaRESUMEN
An erythrocyte isoenzyme of acylphosphatase was purified from bovine red cells. The protein was characterized as regards the kinetic parameters and amino acid sequence. A simple and rapid sequencing strategy, based on a few experiments, was used for reconstructing the primary structure of the enzyme, since the purification procedure gave a very low yield. The length of the polypeptide chain is 100 residues. Comparison with the analogous human isoenzyme indicates that the primary structure is about 90% conserved. The presence of two additional residues at the acetylated N-terminus confirms the hypervariability for this region found in other acylphosphatases.