Alteration of intracellular free calcium and acylphosphatase levels in differentiating SH-SY5Y neuroblastoma cells.
Biochem Mol Biol Int
; 43(3): 633-41, 1997 Oct.
Article
en En
| MEDLINE
| ID: mdl-9352082
Levels of acylphosphatase isoenzymes and free intracellular calcium have been investigated in cultured SH-SY5Y human neuroblastoma cells under stimulation with all-trans retinoic acid and phorbol-12-myristate-13-acetate. Under these conditions morphological and functional characteristics demonstrated the differentiation of SH-SY5Y cells towards neuronal phenotype. Retinoic acid treatment caused a progressive and synchronous increase of the organ common-type acylphosphatase and of free intracellular calcium but not of the muscle-type acylphosphatase. Phorbol-12-myristate-13-acetate treatment gave rise to a peak of the muscle-type acylphosphatase levels during the early differentiation stage whereas organ common-type isoenzyme and free calcium levels show a pattern similar to that observed in retinoic acid-treated cells. These evidences indicate that the two acylphosphatase isoenzymes play different roles in SH-SY5Y differentiation and that during this process the expression of organ common-type acylphosphatase increases in a synchronous way with intracellular free calcium concentration.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Calcio
/
Ácido Anhídrido Hidrolasas
/
Proteínas de Neoplasias
/
Neuroblastoma
Límite:
Humans
Idioma:
En
Revista:
Biochem Mol Biol Int
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Reino Unido