RESUMEN
When bound to Escherichia coli ribosomes and irradiated with near-UV light, various derivatives of yeast tRNA(Phe) containing 2-azidoadenosine at the 3' terminus form cross-links to 23 S rRNA and 50 S subunit proteins in a site-dependent manner. A and P site-bound tRNAs, whose 3' termini reside in the peptidyl transferase center, label primarily nucleotides U2506 and U2585 and protein L27. In contrast, E site-bound tRNA labels nucleotide C2422 and protein L33. The cross-linking patterns confirm the topographical separation of the peptidyl transferase center from the E site domain. The relative amounts of label incorporated into the universally conserved residues U2506 and U2585 depend on the occupancy of the A and P sites by different tRNA ligands and indicates that these nucleotides play a pivotal role in peptide transfer. In particular, the 3'-adenosine of the peptidyl-tRNA analogue, AcPhe-tRNA(Phe), remains in close contact with U2506 regardless of whether its anticodon is located in the A site or P site. Our findings, therefore, modify and extend the hybrid state model of tRNA-ribosome interaction. We show that the 3'-end of the deacylated tRNA that is formed after transpeptidation does not immediately progress to the E site but remains temporarily in the peptidyl transferase center. In addition, we demonstrate that the E site, defined by the labeling of nucleotide C2422 and protein L33, represents an intermediate state of binding that precedes the entry of deacylated tRNA into the F (final) site from which it dissociates into the cytoplasm.
Asunto(s)
Escherichia coli/genética , ARN Bacteriano/metabolismo , ARN Ribosómico 23S/metabolismo , ARN de Transferencia/metabolismo , Conformación de Ácido Nucleico , ARN Bacteriano/química , ARN Ribosómico 23S/química , ARN de Transferencia/químicaRESUMEN
The peptidyl transferase center of the Escherichia coli ribosome encompasses a number of 50S-subunit proteins as well as several specific segments of the 23S rRNA. Although our knowledge of the role that both ribosomal proteins and 23S rRNA play in peptide bond formation has steadily increased, the location, organization, and molecular structure of the peptidyl transferase center remain poorly defined. Over the past 10 years, we have developed a variety of photoaffinity reagents and strategies for investigating the topography of tRNA binding sites on the ribosome. In particular, we have used the photoreactive tRNA probes to delineate ribosomal components in proximity to the 3' end of tRNA at the A, P, and E sites. In this article, we describe recent experiments from our laboratory which focus on the identification of segments of the 23S rRNA at or near the peptidyl transferase center and on the functional role of L27, the 50S-subunit protein most frequently labeled from the acceptor end of A- and P-site tRNAs. In addition, we discuss how these results contribute to a better understanding of the structure, organization, and function of the peptidyl transferase center.
Asunto(s)
Escherichia coli/genética , Peptidil Transferasas/metabolismo , ARN Bacteriano/metabolismo , ARN Ribosómico 23S/metabolismo , ARN de Transferencia/metabolismo , Ribosomas/metabolismo , Sitios de Unión , Peptidil Transferasas/química , Relación Estructura-ActividadRESUMEN
2,6-Diazido-9-(beta-D-ribofuranosyl)purine was prepared by the reaction of 2,6-dichloro-9-(beta-D-ribofuranosyl)purine with sodium azide. The nucleoside was bisphosphorylated with pyrophosphoryl chloride to form 2,6-diazido-9-(beta-D-ribofuranosyl)purine 3',5'-bisphosphate. This product was labeled with 32P using T4 polynucleotide kinase to exchange the 5' phosphate with the gamma phosphate of [gamma-32P]ATP. When yeast tRNA(Phe) containing 2,6-diazido-9-(beta-D-ribofuranosyl)purine at the 3' terminus was bound to the P site of the Escherichia coli ribosome in the presence of poly(U) and irradiated with 300-nm light, the photoreactive tRNA derivative became cross-linked exclusively to the 50S subunit. The label was attached to proteins L27 and L33 as well as to the 23S rRNA.
Asunto(s)
Adenosina/análogos & derivados , Marcadores de Afinidad/síntesis química , Azidas/síntesis química , Reactivos de Enlaces Cruzados/síntesis química , Escherichia coli/química , ARN de Transferencia/química , Ribosomas/química , Adenosina/síntesis química , Centrifugación por Gradiente de Densidad , Escherichia coli/ultraestructura , Concentración de Iones de Hidrógeno , Conformación Molecular , Fotoquímica , ARN de Hongos/química , ARN de Transferencia de Fenilalanina/química , Espectrofotometría Ultravioleta , Sacarosa , Levaduras/metabolismoRESUMEN
Photoreactive tRNA derivatives have been used extensively for investigating the interaction of tRNA molecules with their ligands and substrates. Recombinant RNA technology facilitates the construction of such tRNA probes through site-specific incorporation of photoreactive nucleosides. The general strategy involves preparation of suitable tRNA fragments and their ligation either to a photoreactive nucleotide or to each other. tRNA fragments can be prepared by site-specific cleavage of native tRNAs, or synthesized by enzymatic and chemical means. A number of photoreactive nucleosides suitable for incorporation into tRNA are presently available. Joining of tRNA fragments is accomplished either by RNA ligase or by DNA ligase in the presence of a DNA splint. The application of this methodology to the study of tRNA binding sites on the ribosome is discussed, and a model of the tRNA-ribosome complex is presented.
Asunto(s)
Reactivos de Enlaces Cruzados/química , ARN de Transferencia/química , ARN/química , Fotoquímica , ARN/genética , Sondas ARN , Empalme del ARN , ARN de Transferencia/genéticaRESUMEN
Yeast tRNA(Phe), containing the photoreactive nucleoside 2-azidoadenosine at position 37 within the anticodon loop, has been cross-linked to the aminoacyl-tRNA (A) and peptidyl-tRNA (P) binding sites of the Escherichia coli ribosome. The 30S subunit was exclusively labeled in each case, and cross-linking occurred to both protein and 16S rRNA. Electrophoretic and immunological analyses demonstrated that S7 was the only 30S-subunit protein covalently attached to the tRNA. However, digestion of the A and P site-labeled S7 with trypsin revealed a unique pattern of cross-linked peptide(s) at each site. Thus, while the anticodon loop of tRNA is in close proximity to protein S7 at both the A and P sites, it neighbors a different portion of the protein molecule in each. The placement of the aminoacyl- and peptidyl-tRNA binding sites is discussed in relationship to recent models of the 30S ribosomal subunit.
Asunto(s)
Anticodón/química , ARN de Transferencia de Fenilalanina/química , Proteínas Ribosómicas/química , Proteínas Bacterianas/química , Sitios de Unión , Escherichia coli , Fotoquímica , ARN de Hongos/química , ARN de Transferencia de Fenilalanina/efectos de la radiación , Proteínas Ribosómicas/efectos de la radiación , Saccharomyces cerevisiae/genéticaRESUMEN
Two analogs of the anticodon arm of yeast tRNAPhe (residues 28-43), in which G43 was replaced by the photoreactive nucleosides 2-azidoadenosine and 8-azidoadenosine, have been used to create 'zero-length' cross-links to ribosomal components at the peptidyl-tRNA binding site (P site) of 30 S subunits from the Escherichia coli ribosome. To prepare the analogs, 2-azidoadenosine and 8-azidoadenosine bisphosphates were first ligated to the 3' end of the anticodon-containing dodecanucleotide ACmUGmAAYA psi m5CUG from yeast tRNAPhe. The trinucleotide CAG was then joined to the 5' end of the resulting tridecanucleotide in a subsequent ligation. Both analogs bound to poly(U)-programmed 30 S subunits with affinities similar to that of the unmodified anticodon arm from yeast tRNAPhe. Irradiation of noncovalent complexes containing the photolabile analogs, poly(U) and 30 S ribosomal subunits with 300 nm light led to the covalent attachment of the anticodon arms to proteins S13 and S19. Further analysis revealed that S13 accounted for about 80%, and S19 for about 20%, of the cross-linked material. Labeling of these two proteins with 'zero-length' cross-linking probes provides useful information about the location and orientation of P site-bound tRNA on the ribosome and permits a test of recently proposed models of the three-dimensional structure of the 30 S subunit.
Asunto(s)
Anticodón/metabolismo , Escherichia coli/genética , Sondas de Oligonucleótidos/síntesis química , ARN de Transferencia de Fenilalanina/metabolismo , Ribosomas/metabolismo , Adenosina/análogos & derivados , Marcadores de Afinidad , Azidas , Secuencia de Bases , Sitios de Unión , Escherichia coli/metabolismo , Cinética , Modelos Estructurales , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Ribosomas/efectos de la radiación , Ribosomas/ultraestructuraRESUMEN
Photoreactive derivatives of yeast tRNA(Phe) containing 2-azidoadenosine (2N3A) at position 73 or 76 have been crosslinked to the peptidyl site of Escherichia coli ribosomes. Covalent tRNA-ribosome attachment was dependent upon the replacement of adenosine by 2N3A in the tRNA, irradiation with 300-nm light, and the presence of poly(U). In all cases, the modified tRNAs became crosslinked exclusively to 50S ribosomal subunits. While the tRNA derivative containing 2N3A at position 73 labeled only protein L27, that containing 2N3A at position 76 labeled proteins L15, L16, and L27 as well as a segment of the 23S rRNA. The site of crosslinking in the rRNA was identified as guanosine-1945, which lies within a highly conserved sequence adjacent to a number of modified bases and has not until now been identified at the peptidyltransferase center. On the basis of these results, and previously reported crosslinks from tRNA containing 8-azidoadenosine in the 3'-terminal -A-C-C-A sequence [Wower, J., Hixson, S. S. & Zimmermann, R. A. (1988) Biochemistry 27, 8114-8121], we propose a model for the arrangement of tRNA molecules at the peptidyl and aminoacyl sites that is consistent with most of the information available about the location of the peptidyltransferase center and the decoding domain of the E. coli ribosome.
Asunto(s)
Aciltransferasas/metabolismo , Adenosina/análogos & derivados , Azidas/farmacología , Reactivos de Enlaces Cruzados/farmacología , Escherichia coli/enzimología , Peptidil Transferasas/metabolismo , ARN de Transferencia Aminoácido-Específico/metabolismo , ARN de Transferencia de Fenilalanina/metabolismo , Proteínas Ribosómicas/metabolismo , Ribosomas/enzimología , Adenosina/farmacología , Modelos Estructurales , ARN Ribosómico 23S/metabolismo , ARN de Transferencia/metabolismo , Proteínas Ribosómicas/aislamiento & purificación , Ribosomas/ultraestructura , Relación Estructura-ActividadRESUMEN
2-Azidoadenosine was synthesized from 2-chloroadenosine by sequential reaction with hydrazine and nitrous acid and then bisphosphorylated with pyrophosphoryl chloride to form 2-azidoadenosine 3',5'-bisphosphate. The bisphosphate was labeled in the 5'-position using the exchange reaction catalyzed by T4 polynucleotide kinase in the presence of [gamma-32P]ATP. Polynucleotide kinase from a T4 mutant which lacks 3'-phosphatase activity (ATP:5'-dephosphopolynucleotide 5'-phosphotransferase, EC 2.7.1.78) was required to facilitate this reaction. 2-Azidoadenosine 3',5'-[5'-32P]bisphosphate can serve as an efficient donor in the T4 RNA ligase reaction and can replace the 3'-terminal adenosine of yeast tRNAPhe with little effect on the amino acid acceptor activity of the tRNA. In addition, we show that the modified tRNAPhe derivative can be photochemically cross-linked to the Escherichia coli ribosome.
Asunto(s)
Adenosina Difosfato/análogos & derivados , Azidas , Escherichia coli/metabolismo , ARN de Transferencia/metabolismo , Ribosomas/metabolismo , Adenosina Difosfato/síntesis química , Adenosina Difosfato/metabolismo , Marcadores de Afinidad , Cromatografía en Capa Delgada , Cinética , Fosforilación , Fotoquímica , Polinucleótido 5'-Hidroxil-Quinasa/metabolismo , ARN de Transferencia de Fenilalanina/metabolismo , Fagos T/enzimologíaRESUMEN
A simple method has been developed for the preparation of 5'-32P-labeled 8-azidoadenosine 3',5'-bisphosphate (p8N3Ap) for use in photoaffinity labeling studies. Irradiation of a complex between p8N3Ap and bovine pancreatic ribonuclease A (RNase A) with light of 300-350 nm led to the covalent attachment of the nucleotide to the enzyme. RNase A could also be labeled in the dark with prephotolyzed p8N3Ap. In either case, the nucleotide reacted with the same tryptic peptide, encompassing amino acids 67-85 of the protein. The site of labeling was determined to be either Thr-78 or Thr-82, both of which are close to or at the pyrimidine binding site of the enzyme. This result is consistent with recent nuclear magnetic resonance and X-ray studies which indicate that 8-substituted adenine nucleotides interact with the pyrimidine binding site of RNase A.
Asunto(s)
Adenosina Difosfato/análogos & derivados , Marcadores de Afinidad/metabolismo , Azidas/metabolismo , Ribonucleasa Pancreática/metabolismo , Adenosina Difosfato/síntesis química , Adenosina Difosfato/metabolismo , Azidas/síntesis química , Indicadores y Reactivos , Cinética , Mapeo Peptídico , Radioisótopos de Fósforo , Fotoquímica , TripsinaRESUMEN
The 3'-terminal -A-C-C-A sequence of yeast tRNA(Phe) has been modified by replacing either adenosine-73 or adenosine-76 with the photoreactive analogue 8-azidoadenosine (8N3A). The incorporation of 8N3A into tRNA(Phe) was accomplished by ligation of 8-azidoadenosine 3',5'-bisphosphate to the 3' end of tRNA molecules which were shortened by either one or four nucleotides. Replacement of the 3'-terminal A76 with 8N3A completely blocked aminoacylation of the tRNA. In contrast, the replacement of A73 with 8N3A has virtually no effect on the aminoacylation of tRNA(Phe). Neither substitution hindered binding of the modified tRNAs to Escherichia coli ribosomes in the presence of poly(U). Photoreactive tRNA derivatives bound noncovalently to the ribosomal P site were cross-linked to the 50S subunit upon irradiation at 300 nm. Nonaminoacylated tRNA(Phe) containing 8N3A at either position 73 or position 76 cross-linked exclusively to protein L27. When N-acetylphenylalanyl-tRNA(Phe) containing 8N3A at position 73 was bound to the P site and irradiated, 23S rRNA was the main ribosomal component labeled, while smaller amounts of the tRNA were cross-linked to proteins L27 and L2. Differences in the labeling pattern of nonaminoacylated and aminoacylated tRNA(Phe) containing 8N3A in position 73 suggest that the aminoacyl moiety may play an important role in the proper positioning of the 3' end of tRNA in the ribosomal P site. More generally, the results demonstrate the utility of 8N3A-substituted tRNA probes for the specific labeling of ribosomal components at the peptidyltransferase center.
Asunto(s)
Adenosina/análogos & derivados , Marcadores de Afinidad/farmacología , Azidas/farmacología , Reactivos de Enlaces Cruzados/farmacología , Escherichia coli/metabolismo , Aminoacil-ARN de Transferencia/metabolismo , Ribosomas/metabolismo , Adenosina/farmacología , Conformación de Ácido Nucleico , Fotoquímica , Puromicina/farmacología , Aminoacil-ARN de Transferencia/efectos de los fármacosRESUMEN
The 2-thiocytidine residue at position 32 of tRNA1Arg from Escherichia coli was modified specifically with three photoaffinity reagents of different lengths, and the corresponding N-acetylarginyl-tRNA1Arg derivatives were cross-linked to the P site of E. coli 70S ribosomes by irradiation. Covalent attachment was dependent upon the presence of a polynucleotide template and exposure to light of the appropriate wavelength. From 4% to 6% of the noncovalently bound tRNA became cross-linked to the ribosome as a result of photolysis, and attachment to the P site was confirmed by the reactivity of arginine in the covalent complexes toward puromycin. Analysis of the irradiated ribosomes by sucrose-gradient sedimentation at low Mg2+ concentration revealed that the tRNA was associated exclusively with the 30S subunit in all cases. Two of the N-acetylarginyl-tRNA1Arg derivatives were attached primarily to ribosomal proteins whereas the third was cross-linked mainly to 16S RNA. Partial RNase digestion of the latter complex demonstrated that the tRNA had become attached to the 3' third of the rRNA molecule. In addition, the tRNA-rRNA bond was shown to be susceptible to cleavage by hydroxylamine and mercaptoethanol.
Asunto(s)
Anticodón/metabolismo , Escherichia coli/genética , ARN Ribosómico/efectos de la radiación , Aminoacil-ARN de Transferencia/efectos de la radiación , ARN de Transferencia/metabolismo , Ribosomas/efectos de la radiación , Rayos Ultravioleta , Cinética , Fotoquímica , ARN Ribosómico/metabolismo , Aminoacil-ARN de Transferencia/metabolismo , Ribosomas/metabolismoRESUMEN
The dihydrouracil residue at position 20 of Escherichia coli tRNAGly1 has been replaced by the photoaffinity reagent, N-(4-azido-2-nitrophenyl)glycyl hydrazide (AGH). The location of the substituent was confirmed by the susceptibility of the modified tRNA to cleavage with aniline. When N-acetylglycyl-tRNAGly1 derivatized with AGH was bound noncovalently to the P site of E. coli 70 S ribosomes, 5-6% on average was photochemically cross-linked to the ribosomal particles in a reaction requiring poly(G,U), irradiation and the presence of the AGH label in the tRNA. Approximately two-thirds of the covalently attached tRNA was associated with 16 S RNA in the 30 S subunit. This material was judged to be in the P site by the criterion of puromycin reactivity. As partial RNAase digestion of the tRNA-16 S RNA complex produced labeled fragments from both 5' and 3' segments of the rRNA, there appeared to be more than one site of cross-linking in the 30 S subunit. The small amount of N-acetylglycyl-tRNAGly1 associated with the 50 S subunit was also linked mainly to rRNA, but it was not puromycin-reactive.
Asunto(s)
Aminoacil-ARN de Transferencia/metabolismo , Ribosomas/metabolismo , Marcadores de Afinidad/metabolismo , Azidas/metabolismo , Sitios de Unión , Electroforesis en Gel de Poliacrilamida , Escherichia coli/genética , Conformación de Ácido Nucleico , Fotoquímica , Puromicina/farmacología , Ribonucleasas/metabolismo , Uridina/metabolismoAsunto(s)
Azidas , Enzimas/metabolismo , Acetofenonas , Oxidorreductasas de Alcohol/metabolismo , Animales , Sitios de Unión , Anhidrasas Carbónicas/metabolismo , Gliceraldehído-3-Fosfato Deshidrogenasas/metabolismo , Yodoacetatos , NAD/análogos & derivados , Pepsina A/metabolismo , Conejos , Saccharomyces cerevisiae/enzimología , Sulfanilamidas , PorcinosAsunto(s)
Escherichia coli/metabolismo , ARN Ribosómico/metabolismo , ARN de Transferencia/metabolismo , Ribosomas/metabolismo , Rayos Ultravioleta , Escherichia coli/efectos de la radiación , Glutamatos , Lisina , ARN Ribosómico/efectos de la radiación , ARN de Transferencia/efectos de la radiación , Ribonucleasa T1 , Ribosomas/efectos de la radiaciónAsunto(s)
Marcadores de Afinidad/síntesis química , Aminoacil-ARN de Transferencia/farmacología , Ribosomas/metabolismo , Sitios de Unión , Métodos , Conformación Molecular , N-Formilmetionina , Conformación de Ácido Nucleico , Factores de Iniciación de Péptidos , Fenilalanina , Fotoquímica , ARN de Transferencia/metabolismo , Ribosomas/efectos de los fármacos , Relación Estructura-Actividad , Tiouridina/farmacología , ValinaRESUMEN
The synthesis of the photochemically labile bifunctional reagent p-azidophenacyl bromide (1) is described. This compound may be covalently attached to a known site of an enzyme or other macromolecule by nucleophilic displacement at the alpha-bromo ketone moiety. Subsequent irradiation of the bound reagent gives a nitrene which may insert into a second portion of the enzyme forming a cross-link. Regeant 1 proved to be an excellent inhibitor of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase.