P-Azidophenacyl bromide, a versatile photolabile bifunctional reagent. Reaction with glyceraldehyde-3-phosphate dehydrogenase.
Biochemistry
; 14(19): 4251-4, 1975 Sep 23.
Article
en En
| MEDLINE
| ID: mdl-1237309
The synthesis of the photochemically labile bifunctional reagent p-azidophenacyl bromide (1) is described. This compound may be covalently attached to a known site of an enzyme or other macromolecule by nucleophilic displacement at the alpha-bromo ketone moiety. Subsequent irradiation of the bound reagent gives a nitrene which may insert into a second portion of the enzyme forming a cross-link. Regeant 1 proved to be an excellent inhibitor of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetofenonas
/
Gliceraldehído-3-Fosfato Deshidrogenasas
Límite:
Animals
Idioma:
En
Revista:
Biochemistry
Año:
1975
Tipo del documento:
Article
Pais de publicación:
Estados Unidos