Asunto(s)
Anemia , Coriocarcinoma , Enfermedades en Gemelos , Enfermedades Placentarias , Complicaciones Neoplásicas del Embarazo , Gemelos Dicigóticos , Adulto , Anemia/embriología , Coriocarcinoma/sangre , Femenino , Hemoglobina Fetal/análisis , Humanos , Recién Nacido , Masculino , Embarazo , Gemelos Dicigóticos/sangre , alfa-Fetoproteínas/análisisRESUMEN
We have successfully generated a Drosophila model of human polyglutamine (polyQ) diseases by the targeted expression of expanded-polyQ (ex-polyQ) in the Drosophila compound eye. The resulting eye degeneration is progressive and ex-polyQ dosage- and ex-polyQ length-dependent. Furthermore, intergenerational changes in repeat length were observed in homozygotes, with concomitant changes in the levels of degeneration. Through genetic screening, using this fly model, we identified loss-of-function mutants of the ter94 gene that encodes the Drosophila homolog of VCP/CDC48, a member of the AAA+ class of the ATPase protein family, as dominant suppressors. The suppressive effects of the ter94 mutants on ex-polyQ-induced neurodegeneration correlated well with the degrees of loss-of-function, but appeared not to result from the inhibition of ex-polyQ aggregate formation. In the ex-polyQ-expressing cells of the late pupa, an upregulation of ter94 expression was observed prior to cell death. Co-expression of ter94 with ex-polyQ severely enhanced eye degeneration. Interestingly, when ter94 was overexpressed in the eye by increasing the transgene copies, severe eye degeneration was induced. Furthermore, genetical studies revealed that ter94 was not involved in grim-, reaper-, hid-, ced4-, or p53-induced cell death pathways. From these observations, we propose that VCP is a novel cell death effector molecule in ex-polyQ-induced neurodegeneration, where the amount of VCP is critical. Control of VCP expression may thus be a potential therapeutic target in ex-polyQ-induced neurodegeneration.
Asunto(s)
Apoptosis/fisiología , Proteínas de Ciclo Celular/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/fisiología , Enfermedades Neurodegenerativas/metabolismo , Péptidos , Repeticiones de Trinucleótidos/genética , Adenosina Trifosfatasas , Animales , Apoptosis/genética , Proteínas de Ciclo Celular/química , Modelos Animales de Enfermedad , Drosophila melanogaster/genética , Ojo/crecimiento & desarrollo , Ojo/fisiopatología , Mutación , Enfermedades Neurodegenerativas/inducido químicamente , Enfermedades Neurodegenerativas/genética , Fenotipo , Repeticiones de Trinucleótidos/fisiología , Proteína que Contiene ValosinaRESUMEN
We report herein the case of a ruptured liver abscess that resulted in pneumoperitoneum. A patient with diabetes mellitus presented with symptoms of acute abdomen. The plain abdominal radiograph and computed tomography findings revealed abdominal free air and a gas-containing liver abscess, whereby a diagnosis of a ruptured liver abscess was made. An emergency operation was performed, and the abscess was drained followed by peritoneal lavage and the administration of appropriate antibiotics. To the best of our knowledge, very few cases of spontaneous pneumoperitoneum occurring secondary to the rupture of a gas-containing liver abscess have been encountered in Japan.
Asunto(s)
Absceso Hepático/complicaciones , Neumoperitoneo/etiología , Antibacterianos/uso terapéutico , Complicaciones de la Diabetes , Drenaje , Humanos , Absceso Hepático/patología , Absceso Hepático/cirugía , Masculino , Persona de Mediana Edad , Lavado Peritoneal , Rotura , Tomografía Computarizada por Rayos XRESUMEN
Although IL-10 has been implicated in the pathogenesis of several autoimmune diseases, the mechanisms by which this cytokine mediates inflammatory lesions remain to be elucidated. Exocrine gland destruction is an important early step in the development of Sjögren's syndrome. To better understand the role of IL-10 in Sjögren's syndrome, we made transgenic mice in which the mouse IL-10 gene was regulated by the human salivary amylase promoter. Transgenic expression of IL-10 induced apoptosis of glandular tissue destruction and lymphocyte infiltration consisting primarily of Fas-ligand (FasL)+ CD4+ T cells, as well as in vitro up-regulation of FasL expression on T cells. These data suggest that overexpression of IL-10 in the glands and their subsequent Fas/FasL-mediated bystander tissue destruction is a causal factor in the development of this disease.
Asunto(s)
Interleucina-10/fisiología , Síndrome de Sjögren/etiología , Receptor fas/fisiología , Animales , Proteína Ligando Fas , Humanos , Glicoproteínas de Membrana/fisiología , Ratones , Ratones Transgénicos , Receptores de Antígenos de Linfocitos T alfa-beta/genética , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
BACKGROUND: A novel class of inherited human neurodegenerations is now known to be caused by expanded CAG repeats encoding polyglutamines. Polyglutamine-containing protein fragments have been shown to accumulate as aggregates in the nucleus and in the cytoplasm, and to induce cell death when expressed in cultured cells, leading to the proposal that polyglutamine aggregation is an important step in the pathogenesis. Supporting this, nuclear inclusions containing expanded polyglutamines have been identified in neurones from the brains of patients and in neurones from transgenic mouse models of this class of neural disorders. RESULTS: We analysed the consequences of polyglutamine expression in PC12 neuronal cells. Activated SEK1 accumulated with nuclear but not cytoplasmic polyglutamine aggregations, which consequently triggers cell death. Cell death induced by polyglutamine expression was inhibited by a dominant-negative SEK1 (DN-SEK1), but not by DN-SEK1 tagged with a nuclear export signal. Steady state SEK1 expression itself was enhanced two to three-fold. Nuclearly aggregated polyglutamines, which were identified in PML bodies, co-localized with not only activated SEK1 but also activated c-Jun. We also observed that nuclear inclusion-positive neurones from brains with Huntington's disease expressed SEK1. CONCLUSIONS: This study provides molecular links between the neurodegeneration observed in polyglutamine diseases, cell death signalling kinase cascades and nuclear subdomains related to cell death. We propose that the nuclear PML bodies containing polyglutamine aggregates activate the SEK1-JNK kinase cascade, resulting in the transduction of a death signal.
Asunto(s)
Muerte Celular/fisiología , Núcleo Celular/metabolismo , MAP Quinasa Quinasa 4 , Quinasas de Proteína Quinasa Activadas por Mitógenos/metabolismo , Neuronas/fisiología , Animales , Encéfalo/fisiopatología , Núcleo Celular/ultraestructura , Citoplasma/metabolismo , Activación Enzimática , Proteínas Fluorescentes Verdes , Humanos , Proteínas Luminiscentes/análisis , Proteínas Luminiscentes/genética , Ratones , Ratones Transgénicos , Quinasas de Proteína Quinasa Activadas por Mitógenos/genética , Degeneración Nerviosa/genética , Neuronas/citología , Células PC12 , Péptidos/química , Péptidos/genética , Ratas , Transfección , Repeticiones de Trinucleótidos , Ubiquitinas/metabolismoRESUMEN
It is unclear whether organ-specific autoantigens are critical for the development of primary Sjögren's syndrome (SS). A 120-kilodalton organ-specific autoantigen was purified from salivary gland tissues of an NFS/sld mouse model of human SS. The amino-terminal residues were identical to those of the human cytoskeletal protein alpha-fodrin. The purified antigen induced proliferative T cell responses and production of interleukin-2 and interferon-gamma in vitro. Neonatal immunization with the 120-kilodalton antigen prevented the disease in mice. Sera from patients with SS reacted positively with purified antigen and recombinant human alpha-fodrin protein, whereas those from patients with systemic lupus erythematosus and rheumatoid arthritis did not. Thus, the immune response to 120-kilodalton alpha-fodrin could be important in the initial development of primary SS.
Asunto(s)
Autoantígenos/inmunología , Proteínas Portadoras/inmunología , Proteínas de Microfilamentos/inmunología , Síndrome de Sjögren/inmunología , Secuencia de Aminoácidos , Animales , Artritis Reumatoide/inmunología , Autoanticuerpos/biosíntesis , Autoanticuerpos/inmunología , Autoantígenos/aislamiento & purificación , Proteínas Portadoras/aislamiento & purificación , Células Cultivadas , Modelos Animales de Enfermedad , Humanos , Inmunización , Immunoblotting , Interferón gamma/biosíntesis , Interleucina-2/biosíntesis , Lupus Eritematoso Sistémico/inmunología , Activación de Linfocitos , Ratones , Ratones Endogámicos , Proteínas de Microfilamentos/aislamiento & purificación , Datos de Secuencia Molecular , Especificidad de Órganos , Proteínas Recombinantes de Fusión/inmunología , Glándulas Salivales/inmunología , Síndrome de Sjögren/prevención & control , Linfocitos T/inmunologíaRESUMEN
AIMS: To investigate the possibility of an immune response to retroviral antigens or of detecting retrovirus in Sjögren's syndrome. METHODS: Retroviruses were sought in labial salivary glands and peripheral blood mononuclear cells from patients with Sjögren's syndrome by immunoblotting assay, immunohistochemical assay, polymerase chain reaction (PCR), reverse transcriptase (RT) activity assay, and transmission electron microscopy. RESULTS: Sera from five of 15 patients with Sjögren's syndrome (33%) reacted against p24 group specific antigen (gag) of human immunodeficiency virus (HIV). Labial salivary gland biopsy specimens from seven of the 15 patients with Sjögren's syndrome (47%) contained an epithelial cytoplasmic protein reactive with a monoclonal antibody to p24 of HIV. PCR was performed to detect HIV and human T lymphotropic virus type I (HTLV-I) genes from salivary gland tissues and peripheral blood mononuclear cells from patients with Sjögren's syndrome. Mn2+ dependent, Mg2+ independent RT activity was detected in the salivary gland tissues in three of 10 patients. A-type-like retroviral particles were observed in epithelial cells of salivary glands by transmission electron microscopy. Target genes for HIV and HTLV-I were not found in any of the salivary gland tissues or peripheral blood mononuclear cells from Sjögren's syndrome patients. CONCLUSIONS: The data suggest the presence of an unknown retrovirus similar to HIV in the salivary gland which might be involved in the pathogenesis of a subpopulation in Sjögren's syndrome.
Asunto(s)
Retroviridae/aislamiento & purificación , Glándulas Salivales/virología , Síndrome de Sjögren/virología , Adulto , Anciano , Anciano de 80 o más Años , Western Blotting , ADN Viral/aislamiento & purificación , Femenino , VIH/enzimología , VIH/inmunología , VIH/aislamiento & purificación , Anticuerpos Anti-VIH/sangre , Antígenos VIH/aislamiento & purificación , Anticuerpos Anti-HTLV-I/sangre , Virus Linfotrópico T Tipo 1 Humano/enzimología , Virus Linfotrópico T Tipo 1 Humano/inmunología , Virus Linfotrópico T Tipo 1 Humano/aislamiento & purificación , Humanos , Inmunohistoquímica , Leucocitos Mononucleares/virología , Microscopía Electrónica , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , ADN Polimerasa Dirigida por ARN/metabolismo , Retroviridae/enzimología , Retroviridae/genética , Retroviridae/inmunologíaRESUMEN
We report a case of a giant malignant phyllodes tumor examined by(99m)Tc-ses-tamibi (MIBI) mammoscintigraphy. The patient was a 51 year-old woman who complained of bleeding from a large mass in her right breast. The tumor was well circumscribed, with an ulcerized surface. The accumulation of(99m)Tc-MIBI in the tumor was recognized on(99m)Tc-MIBI scintigraphy. A standard radical mastectomy was performed with a wide margin of skin. The resected specimen measured 20 X 17 X 13 cm, weighed 2100 g and was histologically diagnosed as a malignant phyllodes tumor. The skin defect was reconstructed by a rectus abdominis musculocutaneous flap, with good cosmetic results.(99m)Tc-MIBI scintigraphy may have the potential to distinguish a malignant from benign phyllodes tumors.
RESUMEN
We report a case of a giant malignant phyllodes trmor examinde by &sup99m; Tc-sestamibi (MIBI) mammoscintigraphy. The patient was a 51 yera-old woman who complainedof bleeding from a large mass in her right breast. The tumor was well circumscribed, with an ulcerized surface. The accumulation of &sup99m; Tc-MIBI in the tumor was recognized on &sup99m; Tc-MIBI scintigraphy. A standard radical mastectomy was performed with a wide margin of skin. The resected specimen measured 20x17x13cm, weighed 2100g and was histologically diagnosed as a malignant phyllodes tumor. The skin defect was reconstructed by a rectus abdominis musculocutaneous flap, with good cosmetic results. &sup99m; Tc-MIBI scintigraphy may have the potential to distinguish a malignant from benign phyllodes tumors.
Asunto(s)
Endotelinas/biosíntesis , Hepatectomía/métodos , Trasplante de Hígado/fisiología , Donantes de Tejidos , Alanina Transaminasa/sangre , Aspartato Aminotransferasas/sangre , Presión Sanguínea , Dióxido de Carbono/sangre , Endotelinas/sangre , Familia , Humanos , L-Lactato Deshidrogenasa/sangre , Oxígeno/sangre , Presión Parcial , RadioinmunoensayoRESUMEN
Expression of local cytokine genes including interleukin 10 (IL-10) and IL-12 was analyzed in the salivary gland tissues of MRL/lpr mice with Sjögren's syndrome. We demonstrate a significant role of IL-10 and IL-12 in vivo during development of Sjögren's syndrome in MRL/lpr mice. IL-10 mRNA expression was detected before the onset of disease and was upregulated during the course of autoimmune sialadenitis by RT-PCR. A predominant level of expression of IL-12 mRNA was also detected earlier in the proinflammatory stage of autoimmune sialadenities. Moreover, MHC class II (I-Ak) mRNA was detected before the onset of inflammatory lesions until older ages in the salivary glands of MRL/lpr mice. These results suggest that endogenous IL-10 and IL-12 may play important roles on immune-mediated destruction of the salivary glands during development of organ-specific autoimmunity in MRL/lpr mice.
Asunto(s)
Enfermedades Autoinmunes/fisiopatología , Regulación de la Expresión Génica , Interleucina-10/fisiología , Interleucina-12/fisiología , Glándulas Salivales/metabolismo , Síndrome de Sjögren/fisiopatología , Receptor fas/fisiología , Animales , Enfermedades Autoinmunes/genética , Secuencia de Bases , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Femenino , Antígenos de Histocompatibilidad Clase II/biosíntesis , Antígenos de Histocompatibilidad Clase II/genética , Interleucina-10/biosíntesis , Interleucina-10/genética , Interleucina-12/biosíntesis , Interleucina-12/genética , Ratones , Ratones Endogámicos BALB C , Ratones Mutantes , Datos de Secuencia Molecular , Especificidad de Órganos , Reacción en Cadena de la Polimerasa , ARN Mensajero , Glándulas Salivales/patología , Sialadenitis/etiología , Sialadenitis/genética , Sialadenitis/fisiopatología , Síndrome de Sjögren/genética , Bazo/metabolismo , Bazo/patología , Receptor fas/genéticaAsunto(s)
Hepatectomía/métodos , Trasplante de Hígado/métodos , Núcleo Familiar , Donantes de Tejidos , Adulto , Factores de Edad , Aspartato Aminotransferasas/sangre , Bilirrubina/sangre , Niño , Preescolar , Recuento de Eritrocitos , Femenino , Venas Hepáticas , Humanos , Lactante , Trasplante de Hígado/fisiología , Masculino , Persona de Mediana Edad , Estudios RetrospectivosRESUMEN
The influence of graft size-matching on tissue oxygenation and metabolic capability was studied in living related partial liver transplantations for 47 pediatric patients. Their age ranged from 4 months to 17 years 3 months, their body weight from 4.0 to 58.0 kg, graft weight from 191 to 440 g, and graft weight/recipient body weight ratio from 0.61% to 6.0%. Tissue oxygenation and its heterogeneity were investigated by measuring oxygen saturation of hemoglobin in the liver sinusoid (SO2), coefficient of variation of SO2, and arterial ketone body ratio. The metabolic capacity of the graft was investigated by measuring bilirubin clearance, recovery of cholesterol esterification, and ketone body production. In infants with a relatively large liver graft, both intra- and extracellular oxygenation remained low soon after reperfusion but recovered to the control value by the end of the operation. In adolescent recipients of a relatively small graft, by contrast, synthetic and detoxification capacities were relatively deficient; however, these improved with time. These results indicate that sufficient tissue oxygenation and liver regeneration are essential for successful liver transplantation with relatively large and small grafts, respectively.
Asunto(s)
Trasplante de Hígado/patología , Trasplante de Hígado/fisiología , Consumo de Oxígeno , Adolescente , Bilirrubina/metabolismo , Peso Corporal , Niño , Preescolar , Ésteres del Colesterol/metabolismo , Femenino , Hemoglobinas/metabolismo , Humanos , Lactante , Cuerpos Cetónicos/sangre , Cuerpos Cetónicos/metabolismo , Hígado/metabolismo , Hígado/patología , Regeneración Hepática , Masculino , Tamaño de los Órganos , Donantes de TejidosRESUMEN
We extended the indication for living related partial liver transplantation from pediatric to adult cases. Our first case was a 49-year-old woman with primary biliary cirrhosis. Her sister's left lobe, weighing 280 g, was employed as a graft, and the graft weight/recipient's body weight ratio was calculated as 0.59%. To decrease the metabolic load to the relatively small graft, the total bilirubin was decreased from a maximum value of 75.0 mg/d1 to the most recent preoperative value of 36.2 mg/dl by plasma exchange. Intraoperative recovery of tissue oxygenation and its heterogeneity were satisfactory due to a relatively high blood supply. A postoperative decrease in bilirubin and increase in cholesterol esterification were facilitated, concomitant with regeneration of the graft, which weighed 280 g, to 860 cm3 at 3 weeks. Linear regression analysis with respect to tissue oxygenation and metabolic capacity obtained in pediatric cases were applied to this adult case.
Asunto(s)
Trasplante de Hígado , Hígado/metabolismo , Oxígeno/metabolismo , Niño , Femenino , Humanos , Persona de Mediana EdadRESUMEN
Upregulation of vascular cell adhesion molecule-1 (VCAM-1) has been implicated in various pathological conditions. In this study, we used in situ hybridization to determine the cell types expressing VCAM-1 mRNA in rheumatoid synovium. Synovial tissues from seven rheumatoid arthritis (RA) and four osteoarthritis (OA) patients were examined for VCAM-1 mRNA expression. In situ hybridization as well as immunohistochemical analysis showed that VCAM-1 expression was mainly localized to the hyperplastic synovial lining cells and to a lesser extent endothelial cells and synovial fibroblasts in rheumatoid synovium. VCAM-1 expression in OA is less prominent. Hyperplastic synovial lining cells expressing VCAM-1 were mainly CD13+, CD14+, CD33+ and HLA-DR+. Together with morphological features, this suggests that they are type A macrophage-like synovial cells. Our findings indicate that overexpression of VCAM-1 may contribute to the formation of hyperplastic lining layers in the synovium seen in this disorder.
Asunto(s)
Artritis Reumatoide/inmunología , Osteoartritis/inmunología , ARN Mensajero/metabolismo , Nódulo Reumatoide/inmunología , Membrana Sinovial/inmunología , Molécula 1 de Adhesión Celular Vascular/inmunología , Artritis Reumatoide/patología , Expresión Génica , Humanos , Hibridación in Situ , Osteoartritis/patología , Nódulo Reumatoide/patología , Membrana Sinovial/patología , Molécula 1 de Adhesión Celular Vascular/genéticaRESUMEN
We have analysed the role of ICAM-1 and LFA-1 during development of autoimmune sialadenitis in MRL/lpr mice by direct analysis of RNA obtained from the salivary gland tissues, and the therapeutic effects with antibody administration on adoptive transfer system into SCID mice. The expression of cell adhesion molecules was assessed by using reverse transcriptase polymerase chain reaction (RT-PCR) and Southern blot analysis, and immunohistochemical analysis. Up-regulated expression of ICAM-1 mRNA was observed before the onset of inflammatory lesions in the salivary glands at 1 month and 2 months old, and thereafter LFA-1 mRNA was expressed within the typical inflammatory lesions, resembling human Sjögren's syndrome in MRL/lpr mice. Immunohistochemically, ICAM-1 was localized exclusively in the endothelial cells of varying sized blood vessels before the onset of disease, and LFA-1 expressing inflammatory cells were found within these lesions. When the therapeutic effects in vivo were examined, antibodies to ICAM-1 in combination with anti-LFA-1 prevented adoptive transfer of Sjögren's syndrome in MRL/lpr mice into SCID mice, while no significant effect was found when treated with either antibody. These findings indicate that in Sjögren's syndrome-like autoimmune lesions in MRL/lpr mice the ICAM-1/LFA-1 pathway may play a crucial role in the initiation and subsequent progression of T cell-mediated autoimmunity in the salivary and lacrimal glands of MRL/lpr mice.
Asunto(s)
Molécula 1 de Adhesión Intercelular/inmunología , Antígeno-1 Asociado a Función de Linfocito/inmunología , Sialadenitis/inmunología , Animales , Secuencia de Bases , Cartilla de ADN/química , Femenino , Expresión Génica , Inmunización Pasiva , Molécula 1 de Adhesión Intercelular/genética , Ratones , Ratones Mutantes , Ratones SCID/inmunología , Datos de Secuencia Molecular , ARN Mensajero/genética , Síndrome de Sjögren/inmunología , Síndrome de Sjögren/prevención & controlRESUMEN
In this study, we have identified the source of nitric oxide (NO) produced in the human inflammatory joints by analyzing expression of inducible NO synthase. In ex vivo organ cultures, both inflammatory synovium and cartilage from patients with rheumatoid arthritis produced NO. The NO production was suppressed by NG-monomethyl-L-arginine, an inhibitor of NO synthase. The amount of NO produced by the synovium correlated with the proportion of CD14+ cells in the corresponding tissue (r = 0.8, P < 0.05). Immunohistochemical analysis as well as in situ hybridization showed that inducible NO synthase was predominantly expressed in synovial lining cells, endothelial cells, chondrocytes, and to a lesser extent, in infiltrating mononuclear cells and synovial fibroblasts. The synovial lining cells and the infiltrating cells expressing inducible NO synthase were identified where CD14+ cells were located. Together with morphological features, this suggests that they are type A synoviocytes. NO production from freshly isolated synoviocytes and chondrocytes was up-regulated by in vitro stimulation with a combination of IL-TNF-beta, TNF-alpha, and LPS. In summary, the present results suggest that NO is produced primarily by CD14+ synoviocytes, chondrocytes, and endothelial cells in inflammatory joints of arthritides. NO production can be upregulated by cytokines present in inflamed joints. The increased NO production may thus contribute to the pathological features in inflammatory arthritides.
Asunto(s)
Artritis Reumatoide/metabolismo , Cartílago Articular/metabolismo , Óxido Nítrico Sintasa/biosíntesis , Óxido Nítrico/biosíntesis , Membrana Sinovial/metabolismo , Cartílago Articular/patología , Citocinas/metabolismo , Femenino , Humanos , Receptores de Lipopolisacáridos/metabolismo , Masculino , Persona de Mediana Edad , Técnicas de Cultivo de Órganos , Membrana Sinovial/patología , Regulación hacia ArribaRESUMEN
We measured lymphocyte energy charge (LEC) in septic patients after hepatectomy to clarify the energy metabolism of lymphocytes in relation to arterial ketone body ratio (AKBR) reflecting the hepatic mitochondrial redox potential. Sixteen patients with AKBR above 0.7 (state A) tolerated their operations well without postoperative infectious episodes and their LEC (0.895 +/- 0.005, mean +/- SEM) was significantly (p < 0.001) higher than that (0.841 +/- 0.010) of 9 patients with AKBR from 0.7 to 0.4 (state B). Four of the 9 state B patients had multiple organ failure (MOF) with sepsis as a trigger. AKBR in 7 of 9 state B patients decreased and remained below 0.4 (state C). These state C patients showed significantly reduced LEC (0.781 +/- 0.024; p < 0.001, p < 0.05, compared with that in states A and B, respectively) and finally died of MOF with septic state. These results suggest that the severe and prolonged impairment of energy metabolism in the liver may be accompanied with the metabolic derangement of lymphocytes.
Asunto(s)
Células Sanguíneas/metabolismo , Metabolismo Energético , Hepatectomía , Infecciones/sangre , Linfocitos/metabolismo , Anciano , Anciano de 80 o más Años , Arterias , Femenino , Humanos , Cuerpos Cetónicos/sangre , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias , Sales de Tetrazolio/análisis , Tiazoles/análisisRESUMEN
The effect of the gabexate mesilate (Gab) on thrombin and plasmin generation following liver resection in cirrhotic patients was studied. Six cirrhotic patients received an infusion of Gab after liver resection (Gab group), and another 6 patients did not receive such treatment (Con group). The parameters measured were thrombin-antithrombin complex (TAT), plasmin-antiplasmin complex (PAP) and D-dimer. The real increases of D-dimer and PAP were significantly higher in Con group after surgery while no significant difference was observed in the increase of TAT. These results show that Gab suppresses plasmin generation and following D-dimer production more effectively than thrombin generation after hepatic resection.