In situ hybridization study of vascular cell adhesion molecule-1 messenger RNA expression in rheumatoid synovium.
J Autoimmun
; 8(6): 947-57, 1995 Dec.
Article
en En
| MEDLINE
| ID: mdl-8824717
Upregulation of vascular cell adhesion molecule-1 (VCAM-1) has been implicated in various pathological conditions. In this study, we used in situ hybridization to determine the cell types expressing VCAM-1 mRNA in rheumatoid synovium. Synovial tissues from seven rheumatoid arthritis (RA) and four osteoarthritis (OA) patients were examined for VCAM-1 mRNA expression. In situ hybridization as well as immunohistochemical analysis showed that VCAM-1 expression was mainly localized to the hyperplastic synovial lining cells and to a lesser extent endothelial cells and synovial fibroblasts in rheumatoid synovium. VCAM-1 expression in OA is less prominent. Hyperplastic synovial lining cells expressing VCAM-1 were mainly CD13+, CD14+, CD33+ and HLA-DR+. Together with morphological features, this suggests that they are type A macrophage-like synovial cells. Our findings indicate that overexpression of VCAM-1 may contribute to the formation of hyperplastic lining layers in the synovium seen in this disorder.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoartritis
/
Artritis Reumatoide
/
Nódulo Reumatoide
/
Membrana Sinovial
/
ARN Mensajero
/
Molécula 1 de Adhesión Celular Vascular
Límite:
Humans
Idioma:
En
Revista:
J Autoimmun
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
1995
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido