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Numerous biochemical processes are involved in fruit maturation, such as ethylene production, phenolic compounds accumulation, and antioxidant enzymes production. Therefore, the aim of the present work was the evaluation of ethylene production, and the bioactive compounds change in the exocarp and mesocarp of five peach [Prunus persica (L.)] cultivars during three ripening stages, (1) early ripening (ER), (2) commercial maturation, and (3) full ripening (FR) in order to establish the best stage to harvest each peach variety. The experiment was applied to five peach cultivars growing within an arid bioclimatic environment covering the whole peach production season: two early cultivars, Flordastar and Early Maycrest; one variety of mid-season Rubirich; and two late cultivars, Sweet Cap and O'Henry. Ethylene production, phenolic compounds, and oxidative stress through antioxidant enzyme activities (catalase, peroxidases [PODs] Class III, and ascorbate-POD), malondialdehyde (MDA), and hydrogen peroxide (H2 O2 ) production were determined in the exocarp and mesocarp of peach fruits. The results showed a significant increase in ethylene production during fruit ripening. However, a parallel decrease in the level of phenolic compounds as well as in antioxidant enzyme activities was observed. The FR stage was also characterized by an important accumulation of MDA and H2 O2 . In conclusion, important changes in fruit quality associated with the production level of ethylene were observed. Fruits harvested during the ER stage would be more suitable for delivering to distant markets and more appreciated by the peach industries due to their highest phenolic acid content, best antioxidant enzyme activities, and lowest oxidative stress indicator.

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This study evaluated the effects of the exogenous application of ethylenediaminetetraacetic acid (EDTA), indole-3-acetic acid (IAA) and iron sulfate (FeSO4) upon the phytochemical mechanisms of fenugreek grown under Pb-excess (2000 mg L-1 PbCl2). The results showed that chemical additives of EDTA and IAA as well as FeSO4 improved fenugreek germination parameters. The radicle length and the amylase activity were significantly improved under IAA treatment compared to EDTA and FeSO4. Exogenous FeSO4 was more effective to improving growth parameters. Moreover, the decrease in hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels was noted under all chemical additives especially under IAA application. In addition, it was more effective than EDTA and Fe in increasing catalase, glutathione (GSH), ascorbate peroxidase (APX), flavonoids and phenols while the increment superoxide dismutase (SOD) production was more pronounced under EDTA addition to Pb than other chelators. HPLC analysis revealed that the gallic was the major phenol produced under all chelators addition especially with IAA. In addition, the syringic acid was only produced with exogenous IAA while the quercetin was only detected under EDTA addition. Our results exhibited a higher IAA efficiency than EDTA and FeSO4 in mitigating Pb stress in fenugreek through up-regulated mechanisms of the antioxidant system for reducing reactive oxygen species (ROS) activities and enhancing special phenols.

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Regulated deficit irrigation (RDI) strategies may greatly contribute to save irrigation water, especially in low water availability conditions. The effects of RDI on the growth process, photosynthesis, fruit yield, and some quality traits were assessed for two years on three plum (Prunus salicina Lindl.) cultivars ('Black Diamond', 'Black Gold' and 'Black Star') grown in Midwest Tunisia. The experiment was conducted during two successive seasons (2011-2012). Two water regimes were applied per cultivar during the phase of fruit growth until fruit ripening. Stressed trees receive 50% of the amount of irrigation compared to controls (CI). Several eco-physiological parameters and some pomological criteria were measured, based on the climatic condition (ETo, ETc, and VDP). Results showed that the three cultivars had an aptitude for tolerance for moderate stress with varying degrees of response time to drought stress. Globally, a slight decrease in the gas exchange rate (AN and gs) and the water status (RWC, Ψmin, and Ψos) was registered. Tree yields, size and weight show a slight decrease under water stress. Nevertheless, there was an improvement in the total soluble solid content (SSC) and firmness in same cultivars. Our results proved that the Black Star cultivar was the most tolerant to deficit irrigation, in reason that it maintains a good water status and a high photosynthetic activity.

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The present work was designed to evaluate the effects of two water shortage strategies on the phenolic profile and antioxidants activities of four Prunus persica L. cultivars (Flordastar, Early May crest, Rubirich and O'Henry). Over the course of two successive seasons (2016 and 2017), three different irrigation strategies were tested: full irrigation (FI: 100 % crop evapotranspiration (Etc)), sustained deficit irrigation (SDI: 50 % ETc), and cyclic deficit irrigation (CDI: irrigation at 100 % field capacity with a soil moisture of 50 % field capacity). HPLC-UV/VIS profile of phenolic compounds, enzymatic and non-enzymatic antioxidant activities were assessed in exocarp and mesocarp. The results showed that deficit irrigation improved the content of phenolic compounds and the antioxidant activities. In O'Henry, ascorbate peroxidase activity increased significantly under CDI in exocarp (249 %). In conclusion, most cultivars showed an improvement of the fruit quality under SDI, whereas O'Henry fruits gathered the highest phenolic amounts and displayed the best antioxidant activity under CDI.

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Wheat, durum wheat, is the first cereal cultivated and consumed in Tunisia. Because the dominance of calcareous soils in its agroecological systems, known by their low availability of iron (Fe) inducing Fe chlorosis and limiting crop production, its yield remains low. Therefore, the search for tolerant genotypes is always current. In this context, the physiological behavior of six Tunisian genotypes of durum wheat (salim, karim, razek, khiar, inrat100, and maali) cultivated on calcareous and fertile soils for 2 months in a pot experiment was investigated. A greenhouse was used to conduct experiments under natural light. Plant growth, SPAD index, Fe nutrition, Fe distribution, and photosynthesis were monitored and used to evaluate and discriminate their respective physiological responses. On calcareous soil, results revealed reduced plant growth, active Fe, SPAD index, and net photosynthesis. Genotypic differences in the response of wheat to calcareous-induced Fe deficiency were observed and allowed to classify the genotypes Salim and Karim as relatively tolerant. These genotypes expressed Fe translocation capacity (FeT) up to 3 times, Fe use efficiency for photosynthesis (FeUEAn) up to 1.6 times, and chlorophyll use efficiency for photosynthesis (ChlUEAn) up to 3.5 times greater than that expressed by the other genotypes, particularly inrat100 and maali. Thus, the relative tolerance of Salim and Karim is the result of the high ability of Fe uptake and translocation to shoots to support chlorophyll biosynthesis, photosynthesis, and plant growth as well as an important Fe and chlorophyll use efficiency.

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BACKGROUND: Potato yields and tuber compositions are linked to mechanisms adopted by plants to cope with salinity and often can change after cooking. The current study aimed to evaluate the effects of salinity, variety and cooking method in the composition of potato tubers. Three potato varieties (Spunta, Bellini and Alaska) grown under distinct salt levels (T1: 2.2 ms cm-1 EC, T2: 8.5 ms cm-1 EC before electromagnetic treatment and 6.3 ms cm-1 EC after electromagnetic treatment, T3: 8.5 ms cm-1 EC) were studied. Yield and tuber quality attributes (starch, dry matter, specific density and tuber size) were evaluated. Carotenoids, total and individual phenolics determined by high-performance liquid chromatography (HPLC), relative antioxidant capacity (RAC) and ions content were analyzed, in both raw and water boiled tubers. RESULTS: Tuber yield, starch, dry matter, ions and antioxidants were significantly influenced by the salinity level and variety. The least production and the highest antioxidants were obtained under T3. Antioxidants were influenced by cooking method, the interactions treatment × cooking method and variety × cooking method. Individual phenolic compounds exhibited different response to cooking as quercetin, caffeic acid and catechin decreased significantly after boiling. However, coumaric acid increased in Alaska tubers. CONCLUSION: Salinity level, variety and cooking method are important determinants of tuber yield and composition. Electromagnetic water may be useful to enhance potato production and tuber quality in areas suffering from water salinization. © 2020 Society of Chemical Industry.

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Carotenoids and phenolic profile, antioxidant activity as well as concentrations of selected macronutrients (K, N, Mg, Ca and Na) and micronutrients (Zn, Cu and Mn) in flesh and peel of peach fruit were recorded at two harvest dates. Predominant mineral was potassium, followed by calcium, magnesium and sodium. The concentration of most micronutrients was greater in the peel than in the flesh especially in early season. The concentration of most elements in flesh and peel decreased during fruit maturation. Total carotenoids content varied with respect to the cultivar. ß-cryptoxanthin and ß-carotene were the major carotenoids in both tissues and flesh contain the lowest amounts. Neochlorogenic acid, chlorogenic acid, catechin, epicatechin, gallic acid, rutin, quercetin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, were detected in both peel and flesh, with chlorogenic acid and catechin being the predominant components. Peel extracts showed markedly higher antioxidant activities, when estimated by ABTS or DPPH assays, than the flesh counterparts, consistent with the observed higher phenolic content. Overall, total phenolics levels increased at full ripening stage in both peel and flesh. The results found herein provide important data on carotenoids, phenolic and macro- and micronutrient changes during fruit growth, and emphases peach fruit as a potential functional food.

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Plants can synthesize tens to hundreds of thousands of primary and secondary metabolites with diverse biological properties and functions. Fatty acids (FA), phenolic compounds (PC) and volatile compounds (VC) of flesh and peel from three Prunus persica cultivars were evaluated at the Regional Centre of Agricultural Research--Experimental Farm (Sidi Bouzid, Tunisia) during two maturation stages. Palmitic, oleic and linoleic acids are the most abundant FA in Prunus persica cultivars. A genetic effect on FA composition was observed throughout the two sampling periods. Peel was rich in oleic acid with the highest content (31.3% on total FA) in 'O'Henry' cultivar at the commercial ripening date; flesh was rich in linoleic acid with the highest content (44.7% on total FA) in 'Sweet Cap' cultivar at the full ripening date. The monounsaturated/polyunsaturated fatty acids ratios were higher in the commercial ripe than in the full ripe fruits. The analysis of the composition of the VC led to the characterization of 98 different compounds, showing a very high variability among the cultivars. The full ripe fruit (peel and flesh) exhibited the highest total number of terpenoids. Commercial ripe peels were richest in the percentage of hydrocarbons. Comparing cultivars, 'Sweet Cap' cultivar showed the lowest contents of alcohols in peel and flesh of full ripe fruit but highest in peel of commercial ripe fruit, and lowest content of aldehydes in peel and flesh of commercial ripe fruit but highest in peel of ripe ones and the highest ones of lactones. Among PC, the highest contents were observed for o-diphenols and the values showed varietal influence. Total phenols contents decreased during ripening process (p < 0.05) in both peel and flesh tissues, except found for 'Sweet Cap' cultivar. In conclusion, to achieve better FA composition and greater VC and PC production of the peach fruit, P. persica cultivars should be harvested at the commercial ripening date.

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