RESUMEN
Mitochondrial sequence variants have been associated with many human diseases, including cancer. A well-established experimental strategy to assess the impact of mitochondrial sequence variants is to generate cytoplasmic hybrids (cybrids). Cybridization facilitates the study of mitochondrial DNA (mtDNA) mutations under a controlled nuclear genetic background. However, in generating cybrids, it is important to select most suitable mtDNA donor and recipient cells so that any change in recipient's cellular phenotype may be attributed to the introduction, through the donor, of mutations in the gene of interest. Here we catalog mitochondrial sequence variants, gene expression, and haplogroups across 937 publicly available cancer cell lines curated by the Cancer Cell Line Encyclopedia (CCLE). We then present DoReMi (Donor, Recipient Mitochondrial DNA matching) as a method to score candidate donor and recipient cell lines based on their mtDNA mutational profiles, including their heteroplasmy levels. DoReMi will allow researchers to design successful cybrid experiments for querying the role of mutations in their mitochondrial-encoded gene of interest. Researchers may also apply DoReMi to study their own cell lines. DoReMi and the other resources provided by this study help optimize cybridization experiments. This software is available for use at http://mendel.gene.cwru.edu/laframboiselab/software.php.
Asunto(s)
Técnicas Citológicas/métodos , ADN Mitocondrial/genética , Neoplasias/genética , Hibridación de Ácido Nucleico/métodos , Línea Celular Tumoral , Variación Genética , Haplotipos , Humanos , MutaciónRESUMEN
Quinolinate phosphoribosyltransferase (QPRT), purified from hog liver, has been crystallized using PEG 8000 as the precipitant. The crystals form long hexagonal rods in the space group P6322 with cell dimensions a = b = 121.7, c = 94.5 A. Based on the unit-cell dimensions and the calculated molecular mass of 33 500 Da, the Matthews coefficient suggests one molecule per asymmetric unit (Vm = 3.45 A3 Da-1; 64% solvent). Three native data sets were collected to a resolution of 2.5 A and merged to provide a set that is 94.7% complete, with an Rsym value of 9.6%.
Asunto(s)
Pentosiltransferasa/química , Animales , Cristalización , Cristalografía por Rayos X , PorcinosRESUMEN
The aqueous suspensions of roots of an Indian drug Ashwagandha and the Korean drug Ginseng were tested comparatively for 2 pharmacological activities, namely the anti-stress activity by the 'mice swimming endurance test' and anabolic activity by noting gain in body weights and levator ani muscle in rats. A significant increase in mice swimming time was shown by Ginseng (P < 0.001) and Ashwagandha (P < 0.01) as compared to the control group. Significant increase in body weights in the Ashwagandha treated group (P < 0.05) was better than Ginseng (P < 0.5). Gain in wet weights of the levator ani muscle were also significant in Ginseng (P < 0.001) and Ashwagandha (P < 0.01) treated groups, however, the weight gain of dried levator ani muscles showed comparable results for both these drugs (P < 0.01).