Crystallization and preliminary crystallographic investigation of porcine quinolinate phosphoribosyltransferase.

Moore, D; Bice, T; Jin, L; Grandhi, A; DeLucas, L J; Narayana, S V

Moore, D; Bice, T; Jin, L; Grandhi, A; DeLucas, L J; Narayana, S V.

Afiliación

Moore D; Center for Macromolecular Crystallography, University of Alabama at Birmingham, AL 35294, USA.

Acta Crystallogr D Biol Crystallogr ; 54(Pt 1): 119-20, 1998 Jan 01.

Article en En | MEDLINE | ID: mdl-9761830

RESUMEN

Quinolinate phosphoribosyltransferase (QPRT), purified from hog liver, has been crystallized using PEG 8000 as the precipitant. The crystals form long hexagonal rods in the space group P6322 with cell dimensions a = b = 121.7, c = 94.5 A. Based on the unit-cell dimensions and the calculated molecular mass of 33 500 Da, the Matthews coefficient suggests one molecule per asymmetric unit (Vm = 3.45 A3 Da-1; 64% solvent). Three native data sets were collected to a resolution of 2.5 A and merged to provide a set that is 94.7% complete, with an Rsym value of 9.6%.

Asunto(s)

Pentosiltransferasa/química; Animales; Cristalización; Cristalografía por Rayos X; Porcinos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pentosiltransferasa Límite: Animals Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Año: 1998 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links