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1.
Cell Death Differ ; 23(10): 1658-69, 2016 10.
Artículo en Inglés | MEDLINE | ID: mdl-27315298

RESUMEN

Myogenesis is an important biological process that occurs during both skeletal muscle regeneration and postnatal growth. Growing evidence points to the critical role of microRNAs (miRNAs) in myogenesis. Our analysis of miRNA expression patterns reveal that miRNAs of miR-17-92 cluster are dramatically downregulated in C2C12 cells after myogenesis stimulation, are strongly induced in mouse skeletal muscle after injury and decrease steadily thereafter and are downregulated with age in skeletal muscle during mouse and porcine postnatal growth. However, their roles in muscle developmental processes remain elusive. We show that the miR-17-92 cluster promotes mouse myoblast proliferation but inhibits myotube formation. miR-17, -20a and -92a target the actin-associated protein enigma homolog 1 (ENH1). The silencing of ENH1 increased the nuclear accumulation of the inhibitor of differentiation 1 (Id1) and represses myogenic differentiation. Furthermore, the injection of adenovirus expressing miR-20a into the tibialia anterior muscle downregulates ENH1 and delays regeneration. In addition, the downregulation of miR-17-92 during myogenesis is transcriptionally regulated by E2F1. Overall, our results reveal a E2F1/miR-17-92/ENH1/Id1 regulatory axis during myogenesis.


Asunto(s)
Proteínas Adaptadoras Transductoras de Señales/metabolismo , Diferenciación Celular , Proteína 1 Inhibidora de la Diferenciación/metabolismo , MicroARNs/metabolismo , Proteínas de Microfilamentos/metabolismo , Mioblastos/citología , Mioblastos/metabolismo , Transducción de Señal , Regiones no Traducidas 3'/genética , Animales , Animales Recién Nacidos , Proliferación Celular , Regulación hacia Abajo/genética , Factor de Transcripción E2F1/metabolismo , Humanos , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Modelos Biológicos , Desarrollo de Músculos/genética , Músculo Esquelético/metabolismo , Regeneración , Fracciones Subcelulares/metabolismo
2.
J Dairy Sci ; 98(12): 9001-14, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26476938

RESUMEN

In nonruminants it has been demonstrated that microRNA-24 (miR-24) is involved in preadipocyte differentiation, hepatic lipid, and plasma triacylglycerol synthesis. However, its role in ruminant mammary gland remains unclear. In this study we measured miR-24 expression in goat mammary gland tissue at 4 different stages of lactation and observed that it had highest expression at peak lactation when compared with the dry period. Overexpression or downregulation of miR-24 in goat mammary epithelial cells (GMEC) strongly affected fatty acid profiles; in particular, miR-24 enhanced unsaturated fatty acid concentration. Additional effects of miR-24 included changes in triacylglycerol content and the expression of fatty acid synthase, sterol regulatory element binding transcription protein 1, stearoyl-CoA desaturase, glycerol-3-phosphate acyltransferase mitochondrial, and acetyl-CoA carboxylase. Luciferase reporter assay confirmed that fatty acid synthase is a target of miR-24. Taken together, these results not only highlight the physiological importance of miR-24 in fatty acid metabolism in GMEC, but also laid the foundation for further research on regulatory mechanisms among miR-24 and other microRNA expressed in GMEC.


Asunto(s)
Células Epiteliales/metabolismo , Ácido Graso Sintasas/genética , Glándulas Mamarias Animales/metabolismo , MicroARNs/genética , Triglicéridos/biosíntesis , Acetil-CoA Carboxilasa/genética , Acetil-CoA Carboxilasa/metabolismo , Secuencia de Aminoácidos , Animales , Células Cultivadas , Regulación hacia Abajo , Ácido Graso Sintasas/metabolismo , Femenino , Glicerol-3-Fosfato O-Aciltransferasa/genética , Glicerol-3-Fosfato O-Aciltransferasa/metabolismo , Cabras , Lactancia , Metabolismo de los Lípidos , MicroARNs/metabolismo , Datos de Secuencia Molecular , Estearoil-CoA Desaturasa/genética , Estearoil-CoA Desaturasa/metabolismo , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/genética , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/metabolismo
3.
Arch Biochem Biophys ; 452(2): 119-28, 2006 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-16884678

RESUMEN

Malaria is one of the major parasitic diseases. Current treatment of malaria is seriously hampered by the emergence of drug resistant cases. A once-effective drug chloroquine (CQ) has been rendered almost useless. The mechanism of CQ resistance is complicated and largely unknown. Recently, a novel transmembrane protein, Plasmodium falciparum chloroquine resistance transporter (PfCRT), has fulfilled all the requirements of being the CQ resistance gene. In order to elucidate the mechanism how PfCRT mediates CQ resistance, we have cloned the cDNA from a CQ sensitive parasite (3D7) and tried to express it in Pichia pastoris (P. pastoris) but with unsuccessful results due to AT-rich sequences in the malaria genome. We have therefore, based on the codon usage in P. pastoris, chemically synthesized a codon-modified pfcrt with an overall 55% AT content. This codon-modified pfcrt has now been successfully expressed in P. pastoris. The expressed PfCRT has been purified with immuno metal affinity chromatography (IMAC) and then reconstituted into proteoliposome. It was found that proteoliposomes have a saturable, concentration and time-dependent CQ transport activity. In addition, we found that proteoliposomes with resistant PfCRT(r) (K76T or K76I) showed an increased CQ transport activity compared to liposomes with lipid alone, or proteoliposomes reconstituted with sensitive PfCRT(s) (K76) protein. This activity could be inhibited by nigericin and decreased with the removal of Cl(-). This work suggests that PfCRT is mediating CQR in P. falciparum by virtue of its changes in CQ transport activity depending on pH gradient and chloride ion in the food vacuole.


Asunto(s)
Proteínas de la Membrana/aislamiento & purificación , Proteínas de la Membrana/metabolismo , Pichia/genética , Pichia/metabolismo , Plasmodium falciparum/fisiología , Ingeniería de Proteínas/métodos , Proteínas Recombinantes/aislamiento & purificación , Animales , Clonación Molecular/métodos , Proteínas de Transporte de Membrana , Proteínas Protozoarias
4.
Gene ; 278(1-2): 141-7, 2001 Oct 31.
Artículo en Inglés | MEDLINE | ID: mdl-11707331

RESUMEN

Development and differentiation studies of early human embryos have been severely impeded by general difficulties in obtaining suitable samples. In order to isolate and identify new genes expressed during early human development, we constructed and characterized a PCR-based cDNA library using a 4-week-old chorion-free human embryo. The constructed cDNA library contained 6.3 x 10(6) directional recombinants, and its insert size ranged from 0.4 to 1.8 kb. The cDNA library proportionally represents the mRNA population, containing beta-actin, tPA and LINE1 repetitive sequences at the expected frequencies as in other conventionally constructed and PCR-based cDNA libraries. PCR analyses of the library for specific genes have also revealed the presence of cDNAs for developmentally important genes such as CD59, MCP, Quox-1 and ZNF268. Among the 70 randomly selected cDNA clones, 53% encoded previously known genes, 26% matched with anonymous sequences, and 17% showed no sequence similarity and were designated as human early embryo-specific ESTs. These results demonstrate the sequence complexity and relatively low redundancy of our cDNA library. Furthermore, approximately 40% of those randomly analyzed clones contained full-length encoding regions. To our knowledge, this is the first description of the PCR-based cDNA library from a 4-week-old chorion-free human embryo, and the presence of novel sequences within this library makes it a valuable and unique resource for studying gene expression and regulatory mechanisms that underlie the early process of human embryogenesis.


Asunto(s)
ADN Complementario/genética , Embrión de Mamíferos/metabolismo , Biblioteca de Genes , Clonación Molecular , ADN Complementario/química , Bases de Datos de Ácidos Nucleicos , Electroforesis en Gel de Agar , Etiquetas de Secuencia Expresada , Regulación del Desarrollo de la Expresión Génica , Humanos , ARN Mensajero/genética , ARN Mensajero/aislamiento & purificación , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADN
5.
FEMS Immunol Med Microbiol ; 31(3): 203-9, 2001 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-11720816

RESUMEN

CD59 and membrane cofactor protein (MCP, CD46) are widely expressed cell surface glycoproteins that protect host cells from the effect of homologous complement attack. cDNAs encoding human CD59 and MCP cloned from Chinese human embryo were separately transfected into NIH/3T3 cells resulting in the expression of human CD59 and MCP protein on the cell surface. The functional properties of expressed proteins were studied. When the transfected cells were exposed to human serum as a source of complement and naturally occurring anti-mouse antibody, they were resistant to human complement-mediated cell killing. However, the cells remained sensitive to rabbit and guinea pig complement. Human CD59 and MCP can only protect NIH/3T3 cells from human complement-mediated lysis. These results demonstrated that complement inhibitory activity of these proteins is species-selective. The cDNAs of CD59 and MCP were also separately transfected into the endothelial cells (ECs) of the pigs transgenic for the human DAF gene to investigate a putative synergistic action. The ECs expressing both DAF and MCP proteins or both DAF and CD59 proteins exhibited more protection against cytolysis by human serum compared to the cells with only DAF expressed alone.


Asunto(s)
Antígenos CD/metabolismo , Antígenos CD55/metabolismo , Antígenos CD59/metabolismo , Proteínas del Sistema Complemento/inmunología , Citotoxicidad Inmunológica , Endotelio Vascular/metabolismo , Glicoproteínas de Membrana/metabolismo , Células 3T3 , Animales , Antígenos CD/genética , Antígenos CD55/genética , Antígenos CD59/genética , Proteínas Inactivadoras de Complemento/fisiología , Endotelio Vascular/citología , Cobayas , Humanos , Proteína Cofactora de Membrana , Glicoproteínas de Membrana/genética , Ratones , Plásmidos/genética , Conejos , Especificidad de la Especie , Porcinos , Transfección , Trasplante Heterólogo
6.
Biochim Biophys Acta ; 1518(3): 306-10, 2001 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-11311945

RESUMEN

With the aim of identifying genes involved in early human embryonic development, we have isolated a cDNA clone representing a novel human zinc finger gene ZNF268 from 3 week old human embryo cDNA library using a differential hybridization strategy. The complete cDNA sequence of ZNF268 contained an open reading frame of 2841 nucleotides that encodes a 947 amino acid protein with an N-terminal Krüppel-associated box (KRAB) domain and 24 C-terminal zinc fingers. Northern blot analysis showed that ZNF268 mRNA is mainly expressed in 3-5 week old human embryos suggesting it could play certain roles in the embryogenesis. The gene consists of six exons spanning about 22 kb of genomic DNA. According to the genomic sequence from the HTGS database, the ZNF268 gene is assigned to human chromosome 5.


Asunto(s)
Proteínas de Unión al ADN/genética , Regulación del Desarrollo de la Expresión Génica/genética , Proteínas Represoras , Factores de Transcripción/genética , Dedos de Zinc , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Colon/embriología , Biblioteca de Genes , Edad Gestacional , Corazón/embriología , Humanos , Factores de Transcripción de Tipo Kruppel , Datos de Secuencia Molecular , Alineación de Secuencia
7.
Shi Yan Sheng Wu Xue Bao ; 33(1): 27-34, 2000 Mar.
Artículo en Chino | MEDLINE | ID: mdl-12548849

RESUMEN

In order to isolate novel genes related to early human embryo development and differentiation, a directional cDNA library was constructed from 3-week-old human embryo. Single-pass DNA sequence analysis was used to sequence 47 randomly picked low-abundance cDNA clones. This approach led us to select a clone, L30, showing significant homology with the telomeric-associated DNA and zinc finger protein genes. It is about 3.8 kb in length and contains an open reading frame of notable length within 5'-region, and a tailing signal of AAUAAA and poly (A+) with 39 A in 3'-region. The gene was transcribed in human embryo by Northern blot hybridization and assigned to human chromosome 12 by in situ hybridization.


Asunto(s)
ADN Complementario/genética , Proteínas de Unión al ADN/genética , Telómero/genética , Dedos de Zinc/genética , Clonación Molecular , Sondas de ADN , Etiquetas de Secuencia Expresada , Femenino , Biblioteca de Genes , Humanos , Hibridación in Situ , Análisis de Secuencia de ADN
8.
Carbohydr Res ; 301(1-2): 69-76, 1997 Jun 11.
Artículo en Inglés | MEDLINE | ID: mdl-9228740

RESUMEN

We describe a sensitive and convenient immunoassay for larch arabinogalactan and demonstrate its specificity for larch arabinogalactan. Anti-larch arabinogalactan antiserum is about 10(4) and 10(6) times more selective for detecting larch arabinogalactan than antiserum binds to branch terminal disaccharides consisting of the terminal beta-D-galactosyl residue and the penultimate branch (1-->6)-beta-D-galactosyl residue. It does not bind L-arabinose. The sensitivity of the assay for larch arabinogalactan is less than 0.1 microgram/mL. The application of the assay for measuring arabinogalactan pharmacokinetics in rat blood is illustrated.


Asunto(s)
Galactanos/análisis , Galactanos/sangre , Radioinmunoensayo/métodos , Animales , Receptor de Asialoglicoproteína , Sitios de Unión de Anticuerpos , Unión Competitiva , Carbohidratos/farmacología , Reacciones Cruzadas , Galactanos/inmunología , Glicoproteínas/inmunología , Sueros Inmunes/inmunología , Lectinas , Lectinas de Plantas , Polisacáridos/inmunología , Conejos , Ratas , Receptores de Superficie Celular , Sensibilidad y Especificidad , Árboles
10.
Chin Med J (Engl) ; 108(12): 887-91, 1995 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-8728937

RESUMEN

Graft rejection is a baffling problem in organ transplantation which has so far no successful resolution. Present antirejection therapy is expensive, toxic, susceptible to infection, oncogenesis and atherosclerosis. With the use of 2 extremely histoincompatible strains of rats as model, it was found that when six donor splenocytes preconditioned by an extract from the culture medium of streptomyces caespitosus were injected into the host, after 2 weeks immune tolerance heterotopic transplanted donor hearts could survive normally more than 100 days without using any anti-rejection drug. Their mean survival time was 141.4 +/- 2.56 days, while that in the control group was only 11.7 +/- 3.48 days (P < 0.005). Thus, the present research offers some bright prospects in the area of organ transplantation.


Asunto(s)
Rechazo de Injerto/prevención & control , Trasplante de Corazón , Trasplante Heterotópico , Abdomen , Animales , Trasplante de Células , Supervivencia de Injerto , Tolerancia Inmunológica , Ratas , Ratas Sprague-Dawley , Ratas Wistar , Bazo/citología
11.
Biochemistry ; 33(38): 11586-97, 1994 Sep 27.
Artículo en Inglés | MEDLINE | ID: mdl-7918372

RESUMEN

A systematic effort was made to elucidate the mode of recognition at the inositol 1,4,5-trisphosphate-specific receptor. Eleven D-myo-inositol phosphates were synthesized and tested for Ca(2+)-mobilizing and receptor-binding activities, which included Ins(1,3,4,5,6)P5, Ins(1,2,5,6)P4, Ins(1,3,4,5)P4, Ins(1,3,4,6)P4, Ins(1,4,5,6)P4, Ins(3,4,5,6)P4, Ins(1,3,4)P4, Ins(1,4,5)P3, Ins(1,5,6)P3, Ins(1,4)P2, and Ins(4,5)P2. Of these, Ins(1,4,5)P3, Ins(1,3,4,6)P4, Ins(1,3,4,5)P4, Ins(1,4,5,6)P4, and Ins(4,5)P2 were able to elicit Ca2+ release from rat brain microsomes. Binding experiments suggest that the ability of these polyphosphates to effect Ca2+ mobilization arises from interactions with the Ins(1,4,5)P3-specific receptor. Accordingly, a model accounting for the ligand recognition is proposed. The Ins(1,4,5)P3-binding site is presumably composed of two domains. The anchoring domain binds the 4,5-bisphosphate 6-hydroxy motif. Disruption of this structural feature abolishes the agonist activity. The auxiliary domain exerts long-range interactions with the 1-phosphate, thus enhancing the binding affinity. The stereochemical requirement for this electrostatic interaction is, however, less stringent. Evidence suggests that Ca(2+)-mobilizing inositol phosphates are able to effect productive binding by assuming conformations displaying or mimicking these essential structural features.


Asunto(s)
Canales de Calcio/metabolismo , Cerebelo/metabolismo , Inositol 1,4,5-Trifosfato/metabolismo , Fosfatos de Inositol/metabolismo , Microsomas/metabolismo , Receptores Citoplasmáticos y Nucleares/metabolismo , Adenosina/análogos & derivados , Adenosina/química , Adenosina/metabolismo , Animales , Unión Competitiva , Calcio/metabolismo , Canales de Calcio/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Receptores de Inositol 1,4,5-Trifosfato , Fosfatos de Inositol/química , Fosfatos de Inositol/farmacología , Ligandos , Microsomas/efectos de los fármacos , Ratas , Receptores Citoplasmáticos y Nucleares/efectos de los fármacos , Estereoisomerismo
12.
Bioorg Med Chem ; 2(1): 7-13, 1994 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-7922120

RESUMEN

Two types of structural variants of D-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] were prepared by a chemoenzymatic route. These 6-O-substituted analogues retained the biological activity of Ins(1,4,5)P3, and were able to elicit Ca2+ release from porcine brain microsomes. Moreover, these derivatives allowed the preparation of Ins(1,4,5)P3-based immunogens and affinity matrix which were successfully applied to the preparation and purification of antibodies against Ins(1,4,5)P3. These antibodies displayed discriminative affinity towards Ins(1,4,5)P3, and provide a useful tool to study intracellular Ca2+ mobilization.


Asunto(s)
Calcio/metabolismo , Inositol 1,4,5-Trifosfato/análogos & derivados , Animales , Formación de Anticuerpos , Especificidad de Anticuerpos , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Cromatografía de Afinidad , Técnicas In Vitro , Inositol 1,4,5-Trifosfato/síntesis química , Inositol 1,4,5-Trifosfato/farmacología , Líquido Intracelular/metabolismo , Espectroscopía de Resonancia Magnética , Microsomas/efectos de los fármacos , Microsomas/metabolismo , Conejos , Porcinos
13.
Anal Biochem ; 212(1): 143-9, 1993 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-8368487

RESUMEN

A 13C NMR approach was taken to study the biotransformation of (R)- and (S)-[2-13C,2-2H]ibuprofen-CoA in isolated rat liver mitochondria. The 13C chemical shift induced by 2H substitution for the C-2 of [2-13C,2-2H]-ibuprofen-CoA is about 0.5 ppm relative to the corresponding 1H-bearing thioester. The C-2 resonances for [2-13C]- and [2-13C,2-2H]ibuprofen-CoA are 5 ppm downfield with respect to their acid counterparts. Consequently, this technique permits real time analyses of the metabolic fate of the doubly labeled substrate in a quantitative manner. The initial rate of the epimerization of (S)-[2-13C,2-2H]ibuprofen-CoA relative to the (R)-counterpart by intact mitochondria was estimated to be 1.5, which is in line with the equilibrium constant determined with the purified epimerase. Contrary to the reports by other groups, this 13C NMR study did not find spontaneous hydrolysis of the CoA-thioester upon being exposed to intact mitochondria. Current focus of this investigation is to extend this NMR methodology to the levels of whole cells and live animals to gain a better understanding of the enantioselective metabolism of ibuprofen.


Asunto(s)
Ibuprofeno/metabolismo , Mitocondrias Hepáticas/metabolismo , Animales , Biotransformación , Coenzima A/química , Ibuprofeno/química , Ibuprofeno/farmacocinética , Técnicas In Vitro , Espectroscopía de Resonancia Magnética , Masculino , Ratas , Ratas Wistar , Estereoisomerismo
14.
Biochem Biophys Res Commun ; 183(2): 910-6, 1992 Mar 16.
Artículo en Inglés | MEDLINE | ID: mdl-1550596

RESUMEN

We isolated a cDNA encoding type-1 angiotensin II receptor from a human liver cDNA library. The cDNA had an open reading frame encoding a protein of 359 amino acid residues with a relative Mr of 41,060. The deduced amino acid sequence of the human angiotensin II (Ang II) receptor was 95.3% and 94.2% identical to those of bovine and rat type-1 Ang II receptors, respectively, and had a significant similarity with the G protein-coupled receptor. The rank order of the binding to the receptor expressed in COS-7 cells was Ang II greater than Ang III greater than Ang I. The expression of the Ang II receptor mRNA was detected in human liver, lung, adrenal and adrenocortical adenomas but not in adrenomedullary tumor, pheochromocytoma, by Northern blot analysis.


Asunto(s)
Angiotensina II/genética , Regulación Neoplásica de la Expresión Génica , Receptores de Angiotensina/genética , Secuencia de Aminoácidos , Secuencia de Bases , Unión Competitiva , Clonación Molecular , Humanos , Datos de Secuencia Molecular , Mapeo Restrictivo , Transfección
15.
Front Med Biol Eng ; 4(3): 189-99, 1992.
Artículo en Inglés | MEDLINE | ID: mdl-1419918

RESUMEN

There have been considerable errors in non-invasive continuous beat-by-beat blood pressure (BP) measurement using the pulse wave velocity (PWV). To solve the problem, an in vivo study using 10 dogs was performed. The PWV-BP correlation under intact neuro-humoral factors was compared with that under the factors to be partly eliminated and the PWV-BP regularities were investigated using 10-beat average data. It was confirmed that the vasomotion and the viscoelasticity of vascular smooth muscles are the main elements influencing PWV-BP correlation through changing the elasticity of the arterial wall, and result in a 'shift' and 'zigzag swings' in the PWV-BP relationship so as to lower the PWV-BP correlation. By choosing large arteries and modifying the PWV algorithm, linear correlation coefficients of 0.97 +/- 0.027 and standard errors of the BP estimate of 5.29 +/- 2.84 mm Hg were obtained. The study suggests that the error could be further reduced by simultaneously measuring some related parameters.


Asunto(s)
Determinación de la Presión Sanguínea/métodos , Animales , Viscosidad Sanguínea , Interpretación Estadística de Datos , Perros , Elasticidad , Femenino , Masculino , Músculo Liso Vascular/fisiología , Flujo Pulsátil , Valores de Referencia , Sistema Renina-Angiotensina/fisiología , Reproducibilidad de los Resultados
16.
J Vet Med Sci ; 53(1): 43-8, 1991 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-1650597

RESUMEN

To detect Oncorhynchus masou virus (OMV) in salmonid fish, we prepared recombinant plasmids from two distinct BamHI digests of OMV DNA as a probe. Viral DNAs could be detected two weeks earlier before the virus was isolated from asymptomatic or dead fish. Using the probe, viral DNAs were detected from fish reared in fish hatcheries or fish captured in a river in Hokkaido. The sensitivity limit of detection was estimated to be 10 copies of viral DNA per cell.


Asunto(s)
ADN Viral/análisis , Enfermedades de los Peces/microbiología , Infecciones por Herpesviridae/veterinaria , Herpesviridae/aislamiento & purificación , Salmón , Animales , Autorradiografía , Southern Blotting , Sondas de ADN , Electroforesis en Gel de Agar , Herpesviridae/genética , Infecciones por Herpesviridae/microbiología , Hígado/microbiología , Hibridación de Ácido Nucleico , Mapeo Restrictivo
17.
Yao Xue Xue Bao ; 26(8): 619-21, 1991.
Artículo en Chino | MEDLINE | ID: mdl-1805525

RESUMEN

A new saponin, named smilageninoside, mp 265-7 degrees C, was isolated from rhizomes of Anemarrhena asphdeloides by conventional method. The structure of smilageninoside was identified on the basis of IR, 1HNMR, 13CNMR and FAB-MS. Its structure was smilagenin 3-O-[beta-D-glucopyranosyl (1----2)]-beta-D-mannopyranoside.


Asunto(s)
Medicamentos Herbarios Chinos/química , Manósidos/aislamiento & purificación , Espirostanos/aislamiento & purificación , Manósidos/química , Espirostanos/química
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