RESUMEN
The effects of formulation, starter culture and fermentation temperature on growth and synthesis of toxin A (SEA) and TNase by Staphylococcus aureus during fermentation and drying of Spanish chorizo were investigated. Inhibitory factors able to inhibit SEA synthesis in culture media were unable to prevent SEA production in chorizo fermented at 20 and 30°C, though a lower temperature and starter culture SP318 (Lactobacillus sake, Pediococcus pentosaceous and Staphylococcus xylosus) decreased staphylococcal growth and SEA formation. Reduction and even disappearance of the SEA during ripening was observed. In most batches, TNase was a reliable indicator of staphylococcal growth and SEA production. Dextrose added to the salchichón formulation repressed S. aureus growth during drying. Lactobacillus curvatus in combination with dextrose was an effective anti-staphylococcal agent during fermentation.
RESUMEN
The effect of meat cultures (non lactic acid bacteria) on the growth and production of enterotoxins and thermonuclease by Staphylococcus aureus was studied. Micrococcus varians did not affect growth nor the synthesis of metabolites. Levels of enterotoxins A, B and D produced by the respective S. aureus strains were reduced by S. xylosus, S. saprophyticus and S. carnosus. The two latter species prevented production of enterotoxin C(1) and S. xylosus markedly reduced the amount produced. The three coagulase-negative staphylococci showed little inhibitory effect on the growth of S. aureus. Penicillium nalgiovense did not show inhibitory activity against the four S. aureus strains. Debaryomyces hansenii slightly inhibited growth of the enterotoxin A-producing strain, but reduced enterotoxin synthesis at 30 °C. Thermonuclease was detected whenever enterotoxins were detected though the influence of the effector organism was dependent on the test strain.
RESUMEN
One hundred Gram-positive, catalase-positive strains were isolated from freshly dressed lamb carcasses. They were randomly selected from a non-selective medium and tested for 75 characters. Only nine cultures could be identified by conventional methods. A numerical taxonomic study was conducted on the whole population and 25 reference strains. At the 80% similarity level (Ssm), ten clusters were formed. Five of them were entirely composed of reference strains. Phena V and VIII contained seven isolates and two reference strains of Micrococcus. Phena VI (six unidentified isolates), VII (nine staphylococci) and IX (69 unidentified isolates) were more related to M. kristinae than to the remaining reference strains. Properties with possible implications in meat spoilage were: strong lipolytic activity (76%), anaerobic growth (85%), tolerance to 15% (w/v) NaCl (95%) and ability to grow at 15 degrees C (95%) and 4 degrees C (26%).