RESUMEN
Fumonisin B1 (FB1) and aflatoxin B1 (AFB1) are fungal metabolites that frequently co-occur in foodstuffs and are responsible for mycotoxicosis and several primary cancers. Cinnamon essential oil (CEO) has a spacious range of benefit effects but also has some limitations owing to its strong taste or its interaction with some drugs. This study aimed to use the cinnamon oil emulsion droplets (COED) for the protection against oxidative stress, cytotoxicity, and reproductive toxicity in male Sprague-Dawley rats sub-chronically exposed to FB1 and/or AFB1. The composition of CEO was identified using GC-MS then was encapsulated using whey protein as wall material. Male rats were divided into eight groups and treated orally for 8 weeks as follows: control group, AFB1-trreated group (80 µg/kg b.w), FB1-treated group (100 mg/kg b.w), FB1 plus AFB1-treated group, and the groups treated with COED plus FB1 and/or AFB1. Blood and samples of the kidney, liver, and testis were collected for different analysis and histopathological examination. The GC-MS analysis revealed that cinnamaldehyde, α-copaene, trans-cinnamaldehyde, caryophyllene, and delta-cadinene were the main compounds in COE. The average size of COED was 235 ± 1.4 nm and the zeta potential was - 6.24 ± 0.56. Treatment with FB1 and/or AFB1 induced significant disturbances in the serum biochemical analysis, oxidative stress parameters, DNA fragmentation, gene expression, and testosterone and severe pathological changes in the tested organs. Moreover, treatment with both mycotoxins induced synergistic toxic effects. COED did not induce toxic effects and could normalize the majority of the tested parameters and improve the histological picture in rats treated with FB1 and/or AFB1. It could be concluded that COED induce potential protective effects against the single or combined exposure to FB1 and AFB1.
Asunto(s)
Aflatoxina B1/toxicidad , Cinnamomum zeylanicum/química , Fumonisinas/toxicidad , Aceites de Plantas/farmacología , Sustancias Protectoras/farmacología , Animales , Fragmentación del ADN , Cromatografía de Gases y Espectrometría de Masas , Riñón/efectos de los fármacos , Hígado/efectos de los fármacos , Masculino , Aceites Volátiles/análisis , Aceites Volátiles/química , Aceites Volátiles/farmacología , Estrés Oxidativo/efectos de los fármacos , Aceites de Plantas/análisis , Aceites de Plantas/química , Ratas Sprague-Dawley , Testículo/efectos de los fármacos , Pruebas de Toxicidad Subcrónica , Proteína de Suero de Leche/químicaRESUMEN
Production and evaluation of different diet formulas fortified with oyster shell for the prevention and treatment of osteoporosis. Eighty-eight female albino rats were recruited and divided into 11 groups (8 rats each). Group 1 represented negative control while the remaining groups were ovariectomized. Group 2 acted as positive control. Groups 3-5 were fed on basal diet. Groups 6-8 were fed on lentil soup while groups 9-11 were fed on vegetable soup. Group 4, 7, 10 were fed on diets fortified with oyster shell. Groups 5, 8 and 11 were fed on diet formulas fortified with calcium citrate. All calcium fortified diet formulas, especially lentil soup, have minimized risk factors associated with osteoporosis as indicated from the significant increase in tibial weight, total protein, total calcium and phosphorus with noticeable reduction in ALP activity compared to positive group. Maximum recovery was observed for diet fortified with oyster shell. These data suggest that food products fortified with oyster shell as natural and inexpensive source could be beneficial for the prevention and treatment of osteoporosis.
RESUMEN
BACKGROUND/AIMS: The aim of the present study is to probe the potential association between previously-reported GARP2 mutations and retinitis pigmentosa (RP) using Scottish RP patients and controls. METHODS: Exons 4, 5 and 8 in DNA from blood or buccal samples (130 autosomal recessive and simplex RP patients, 31 controls) were amplified and analysed for single-strand conformational polymorphism by capillary electrophoresis (CE-SSCP) and confirmed by sequencing. RESULTS: The p.Arg86Gln mutation in exon 4 was found in just one patient (out of 130), and in 10 of the 31 unaffected subjects. All of these occurrences were in people of West African origin (patient and controls). Two polymorphisms in exon 5, p.His100Arg and p.Gly109Gly, and a c.534+20A>G change in the intronic region flanking the 3' end of exon 8 were also found not to be associated with RP. CONCLUSIONS: The Scottish population examined here had no mutations in the GARP2 exons surveyed that could be associated with RP. The p.Arg86Gln mutation actually appears to be a polymorphism common in ethnic West Africans and not associated with RP. This change may provide a useful marker for West African ancestry.
Asunto(s)
Sustitución de Aminoácidos , Población Negra/genética , Polimorfismo de Nucleótido Simple , África Occidental , Secuencia de Aminoácidos , Secuencia de Bases , Estudios de Casos y Controles , Canales Catiónicos Regulados por Nucleótidos Cíclicos , Exones , Humanos , Datos de Secuencia Molecular , Retinitis Pigmentosa/genética , Escocia , Alineación de Secuencia , Población Blanca/genéticaRESUMEN
DNA microarrays are considered by many researchers to be the platform of choice for the high-throughput analysis of nucleic acids. Since the past two decades, they have been used constantly as powerful tools in differential gene expression, SNP genotyping, DNA sequencing, gene discovery, disease diagnostic and pathways reconstruction. Several methods have been developed to enable samples of limited amounts of RNA to be quantified. Here we evaluate classical and up-to-date assays made available for labelling those samples. This review also sheds light on the recently developed strategies that ensure high sensitivity such as sample and signal amplification, quantum dot, surface plasmom resonance, nanoparticles and cationinc polythiophenes.