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2.
Plant Physiol Biochem ; 111: 59-66, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-27914320

RESUMEN

Cocoa butter (CB) is composed of 96% palmitic, stearic, oleic, linoleic and linolenic fatty acids that are responsible for the hardness, texture and fusion properties of chocolate. Through in vitro plant cell culture it is possible to modify CB lipid profiles and to study the fatty acid biosynthesis pathway on a subcellular level, evaluating fundamental aspects to enhance in vitro fatty acid production in a specific and controlled way. In this research, culture media was supplemented with acetate, biotin, pyruvate, bicarbonate and glycerol at three different concentrations and the effects on the biomass production (g/L), cell viability, and fatty acids profile and production was evaluated in in vitro cell suspensions culture. It was found that biotin stimulated fatty acid synthesis without altering cell viability and cell growth. It was also evident a change in the lipid profile of cell suspensions, increasing middle and long chain fatty acids proportion, which are unusual to those reported in seeds; thus implying that it is possible to modify lipid profiles according to the treatment used. According to the results of sucrose gradients and enzyme assays performed, it is proposed that cacao cells probably use the pentose phosphate pathway, mitochondria being the key organelle in the carbon flux for the synthesis of reductant power and fatty acid precursors.


Asunto(s)
Cacao/citología , Cacao/metabolismo , Ácidos Grasos/biosíntesis , Biomarcadores/metabolismo , Cacao/enzimología , Proliferación Celular , Tamaño de la Célula , Supervivencia Celular , Células Cultivadas , Medios de Cultivo/química , Orgánulos/metabolismo
3.
Int J Oral Maxillofac Surg ; 42(9): 1054-9, 2013 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-23684075

RESUMEN

Fracture fixation using adhesive is a promising alternative in craniofacial surgeries, replacing the plates and screws system. The advantages include the ease of application and avoidance of drilling holes that may weaken the bone and cause fractures. In this study the bond strengths of selected adhesives were evaluated and compared with resorbable plates and screws. Four adhesives, octyl-cyanoacrylate, N-butyl-cyanoacrylate, a novel methyl-methacrylate, and a novel cyanoacrylate derivative, were tested for their microtensile and shear bond strengths. The bone samples were cut into rectangular bars and bonded with selected adhesives for microtensile testing. For the shear bond test, paired bars were bonded at the overlap, while two other sets of bars were attached by a Lactosorb plate using either adhesive or screws. Data were analysed by analysis of variance (ANOVA). The microtensile bond strengths of N-butyl-cyanoacrylate, novel cyanoacrylate derivative, and novel methyl-methacrylate derivative were significantly greater than octyl-cyanoacrylate. When bone sections were fixed with resorbable plates and adhesives, shear bond strength was significantly greater for N-butyl-cyanoacrylate than plate and screws, while the bond strengths of other adhesives were comparable with the plate and screws. N-Butyl cyanoacrylate was shown to have the greatest potential for fixation of fractured bone in craniofacial surgical applications.


Asunto(s)
Cementos para Huesos/química , Implantes Absorbibles , Placas Óseas , Tornillos Óseos , Huesos/patología , Cadáver , Cianoacrilatos/química , Enbucrilato/química , Humanos , Ácido Láctico/química , Ensayo de Materiales , Metilmetacrilato/química , Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Resistencia al Corte , Estrés Mecánico , Resistencia a la Tracción
4.
Rev Enferm ; 30(6): 18-22, 2007 Jun.
Artículo en Español | MEDLINE | ID: mdl-17685141

RESUMEN

This article is a preview of a project whose objective is to carry out a bibliographical analysis of the articles bearing scientific information included in the "ROL de Enfermeria" magazine over the past five years. This magazine started publication in 1978 and has as its goal contributing to the spread of scientific knowledge in the Nursing field.


Asunto(s)
Bibliometría , Enfermería/estadística & datos numéricos , Edición/estadística & datos numéricos , Humanos
5.
Rev. Rol enferm ; 30(6): 418-422, jun. 2007. tab
Artículo en Español | IBECS | ID: ibc-79750

RESUMEN

Este artículo es un avance de un proyecto que tiene como objetivo realizar un análisis bibliométrico de la producción científica de la Revista ROL de Enfermería en los últimos cinco años. La Revista ROL de Enfermería comenzó en el año 1978 y tiene como objetivo contribuir a la difusión del conocimiento científico en Enfermería(AU)


This article is a preview of a project whose objective is to carry out a bibliographical analysis of the articles bearing scientific information included in the «ROL de Enfermeria» magazine over the past five years. This magazine started publication in 1978 and has as its goal contributing to the spread of scientific knowledge in the Nursing field(AU)


Asunto(s)
Humanos , 50088 , Publicaciones Periódicas como Asunto/estadística & datos numéricos , Enfermería/tendencias , Estudios de Evaluación como Asunto , Bibliometría , Bases de Datos Bibliográficas
6.
Curr Microbiol ; 43(3): 209-14, 2001 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-11400072

RESUMEN

Campylobacter jejuni has been reported to produce different toxins that may modulate the immune response in both animals and humans. The effect of C. jejuni enterotoxin on the immune response was investigated in two groups of Balb/c mice. One of them was inoculated intraperitoneally with 1010 colony forming units (CFU) of an enterotoxigenic strain (CCUG 7580), and the second one with a non-enterotoxigenic strain (CCUG 7440). The number of polymorphonuclear (PMN) cells from spleen increased in both enterotoxigenic and non-enterotoxigenic strains as a consequence of C. jejuni infection. Notwithstanding, lymphocyte proliferation stimulated by lipopolysaccharide (LPS) was increased by both enterotoxigenic and non-enterotoxigenic strains. Interleukin-2 (IL-2) production from splenic cells was increased significantly by infection with the enterotoxigenic strain. Both enterotoxigenic and non-enterotoxigenic strains reduced the splenic response to sheep erythrocytes; the response was significantly suppressed for immunoglobulin M (Ig M) and for immunoglobulin G (Ig G) synthesis. These results suggest that C. jejuni is able to modify some components of the immune response in mice, and also that the enterotoxigenic strain has more immunomodulating activity than the non-enterotoxigenic strain.


Asunto(s)
Formación de Anticuerpos , Infecciones por Campylobacter/inmunología , Campylobacter jejuni/inmunología , Enterotoxinas/fisiología , Leucocitos/inmunología , Animales , Toxinas Bacterianas/biosíntesis , Campylobacter jejuni/metabolismo , Campylobacter jejuni/patogenicidad , Enterotoxinas/biosíntesis , Inmunoglobulinas/biosíntesis , Interleucina-2/biosíntesis , Recuento de Leucocitos , Activación de Linfocitos , Linfocitos/inmunología , Ratones , Ratones Endogámicos BALB C , Monocitos/inmunología , Neutrófilos/inmunología , Bazo/citología , Bazo/inmunología
7.
Int J Antimicrob Agents ; 15(3): 193-9, 2000 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-10926441

RESUMEN

Recent studies have suggested that antibiotics may act as biological response modifiers. In this study we investigated the effect of aztreonam, a monobactam antibiotic, on different parameters of acquired immunity in BALB/c mice. Different dosages of aztreonam injected into mice induced an increase in the lymphoproliferative response to specific mitogens and in the production of interleukin-2 by splenic cells, as well as a decreased response of this immune population to sheep erythrocytes lower total blood cell counts and a lower percentage of monocytes than in untreated mice. These results show a modulatory action of aztreonam on different immune parameters, which is independent of its antimicrobial activity and that could be of interest in human therapy.


Asunto(s)
Aztreonam/farmacología , Inmunidad/efectos de los fármacos , Factores Inmunológicos/farmacología , Monobactamas/farmacología , Animales , Formación de Anticuerpos/efectos de los fármacos , Inmunidad Celular/efectos de los fármacos , Interleucina-2/biosíntesis , Leucocitos Mononucleares/inmunología , Masculino , Ratones , Ratones Endogámicos BALB C , Bazo/inmunología
8.
Inorg Chem ; 39(5): 975-9, 2000 Mar 06.
Artículo en Inglés | MEDLINE | ID: mdl-12526377

RESUMEN

A reinvestigation of the NMR spectra of the complexes (NBu4)2[M2(mu-LL)2R4] (M = Pd, Ni, Pt, LL = pyrazolate (pz), 3,5-dimethylpyrazolate (dmpz), 3-methylpyrazolate (mpz), indazolate (indz), R = C6F5; M = Pd, LL = pz, dmpz, mpz, indz, R = 2,4,6-C6F3H2) shows that the boat-shaped dimeric structures of their anions are quite stable in solution, and the previously proposed fast equilibria or dissociations to give species such as [R2M(N-N)(acetone)]-, [R2M(acetone)2] + 2dmpz-, or [R2M(N1-N2)(acetone)]- + [R2M(N2-N1)(acetone)]- in no case occur. A mixture of the two diastereoisomers (head-to-head, HH, and head-to-tail, HT) is present for the asymmetrically substituted azolates (mpz and indz), in a ratio ranging from 1:7 to 1:30 for the different complexes. Strong through-space coupling between the endo ortho fluorine nuclei of different MR2 fragments is observed in the 19F NMR spectra of these diastereoisomers whose boatlike structures place these atoms at short distances.

9.
J Antimicrob Chemother ; 44(4): 561-4, 1999 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-10588322

RESUMEN

The immunomodulating properties of antimicrobial drugs may have important implications for clinical practice, particularly for those patients whose immune system has been compromised. In this study, we assessed the influence of different treatments with a beta-lactam antibiotic (imipenem/cilastatin) on several acquired immune responses of BALB/c mice; splenocyte responses to specific mitogens and to sheep red blood cells, IL-2 production and proportions of the different lympho-monocytic populations. Impenem/cilastatin was shown to modify some lymphocyte-associated immune functions and it would be useful to investigate whether immunomodulatory effects also occur in humans.


Asunto(s)
Quimioterapia Combinada/farmacología , Inmunidad/efectos de los fármacos , Animales , Cilastatina/farmacología , Combinación Cilastatina e Imipenem , Combinación de Medicamentos , Imipenem/farmacología , Interleucina-2/biosíntesis , Activación de Linfocitos/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos BALB C
10.
Int J Antimicrob Agents ; 13(1): 41-6, 1999 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-10563409

RESUMEN

The influence of the dose and the duration of treatment with aztreonam, a monocyclic beta-lactam antibiotic, on the natural immune response of mice has been investigated. The results show the effects induced by the antibiotic on several immune parameters were affected by the duration of treatment. Thus, treatment with 28 mg/kg per day of aztreonam over 14 days increased every immune parameter tested, while treatment with 57 mg/kg per day of aztreonam for 7 days only enhanced the natural killer (NK) activity of splenocytes. Since aztreonam does not apparently impair the innate immune response, it might be a suitable therapy for the treatment of patients who are immunosuppressed.


Asunto(s)
Aztreonam/farmacología , Inmunidad Innata/efectos de los fármacos , Monobactamas/farmacología , Animales , Interleucina-1/biosíntesis , Células Asesinas Naturales/efectos de los fármacos , Células Asesinas Naturales/metabolismo , Masculino , Ratones , Ratones Endogámicos BALB C , Fagocitos/efectos de los fármacos , Fagocitos/metabolismo
11.
Curr Microbiol ; 39(3): 129-33, 1999 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-10441725

RESUMEN

The effect of several Campylobacter jejuni strains on the immune response was analyzed in mice after intraperitoneal inoculation with 10(10) colony forming units (CFU). Three C. jejuni strains were assayed: CCUG 6968 (enterotoxigenic), CCUG 7580 (enterotoxigenic), and CCUG 7440 (non-enterotoxigenic). These C. jejuni strains induced a peritoneal inflammatory response and an important increase in the peritoneal phagocyte oxidative activity measured by chemiluminescence assay, as well as an increase in the number of peritoneal cells. Both interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) production by peritoneal cells were not modified. However, C. jejuni 7440 caused a statistically significant increase in TNFalpha production. These results have demonstrated that different strains of C. jejuni induce an increase of the inflammatory response without a significant cytokine release. However, these infectious microorganisms may be eliminated efficiently by murine macrophages after phagocytosis.


Asunto(s)
Infecciones por Campylobacter/inmunología , Campylobacter jejuni/inmunología , Citocinas/metabolismo , Animales , Líquido Ascítico/citología , Células CHO/efectos de los fármacos , Infecciones por Campylobacter/microbiología , Recuento de Células , Cricetinae , Enterotoxinas/metabolismo , Histocitoquímica , Interleucina-1/metabolismo , Ratones , Ratones Endogámicos BALB C , Fagocitosis , Organismos Libres de Patógenos Específicos , Factores de Tiempo , Factor de Necrosis Tumoral alfa/metabolismo
12.
FEMS Immunol Med Microbiol ; 24(1): 35-42, 1999 May.
Artículo en Inglés | MEDLINE | ID: mdl-10340710

RESUMEN

The effect of nisin on the immune response of mice was studied. Nisin (in the form of the commercial preparation Nisaplin) was incorporated in the diet of experimental mice which were fed for 30, 75 or 100 days. Short-term administration of diets containing Nisaplin induced an increase of both CD4 and CD8 T-lymphocyte cell counts and also a decrease of B-lymphocyte counts. After prolonged diet administration, T-cell counts returned to control levels. Normal levels of B-lymphocytes were also reached after prolonged administration of the lower (but not the higher) Nisaplin concentration. The macrophage/monocyte fraction isolated from peripheral blood became significantly increased after long-term administration (100 days) of Nisaplin-containing diets in a concentration-dependent way. Although the number of peritoneal cells was not affected by the diets, the phagocytic activity of peritoneal cells decreased after prolonged administration of low (but not high) Nisaplin doses.


Asunto(s)
Conservantes de Alimentos , Nisina/inmunología , Animales , Relación CD4-CD8/efectos de los fármacos , Relación CD4-CD8/métodos , Relación Dosis-Respuesta Inmunológica , Citometría de Flujo , Recuento de Leucocitos/efectos de los fármacos , Leucocitos Mononucleares/efectos de los fármacos , Leucocitos Mononucleares/inmunología , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/inmunología , Ratones , Ratones Endogámicos BALB C , Fagocitos/inmunología , Fagocitos/fisiología , Factores de Tiempo
13.
J Nutr Sci Vitaminol (Tokyo) ; 44(1): 57-67, 1998 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-9591234

RESUMEN

Previous studies have demonstrated that dietary lipid manipulation may modify immune response by affecting lymphocyte proliferation, phagocytosis, cytokine production, etc. In this paper, we investigated the effect of olive oil (OO) on the phagocytic activity and cytokine production by murine peritoneal cells. These results were compared with those obtained from mice fed diets containing sunflower oil (SO) or hydrogenated coconut oil (HCO). Balb/c mice were divided into three groups and fed diets containing 15% by weight of either OO, SO or HCO for 5, 15, 30, 60 or 90 d. Phagocytic activity and interleukin-1 (IL-1) production were increased in OO-fed mice as compared to the other groups. On the contrary, no significant differences were observed in the levels of tumor necrosis factor (TNF) production, although the levels of this cytokine were slightly increased in mice fed the OO diet. These observations suggest that OO is able to modify the immune response and therefore, it may be used as an immunomodulatory agent.


Asunto(s)
Citocinas/biosíntesis , Grasas Insaturadas en la Dieta/administración & dosificación , Ácidos Grasos/administración & dosificación , Peritoneo/citología , Peritoneo/inmunología , Fagocitosis , Animales , Peso Corporal , Recuento de Células , Interleucina-1/biosíntesis , Ratones , Ratones Endogámicos BALB C , Aceite de Oliva , Aceites de Plantas , Factor de Necrosis Tumoral alfa/biosíntesis
14.
Folia Biol (Praha) ; 44(3): 117-21, 1998.
Artículo en Inglés | MEDLINE | ID: mdl-10730853

RESUMEN

A mixture of Ficoll 400 and sodium diatrizoate (Hypaque) at a density of 1.077 g/ml has been used to isolate the mononuclear cells from the remaining haematic cells. A simple, inexpensive and classical method was established to obtain substantially erythrocyte-free polymorphonuclear cell preparations from mouse peripheral blood, using a mixture of the same substances but at a density of 1.119 g/ml. This method along with that at a density of 1.077 g/ml allows two cellular bands to appear which contain mononuclear and polymorphonuclear (PMN) cells, respectively. Using this method, the counts of monocytes isolated from peripheral blood are significantly greater than those obtained by a one-step Ficoll-Hypaque procedure. On the contrary, the counts of PMN cells are significantly smaller than when sedimentation in dextran (6% solution) is used after gradient centrifugation. In this paper, chemiluminescence assay has been used to analyze the possible variations in phagocytic activity of cells isolated by both procedures, since it appears to be one of the most sensitive methods available for this purpose. The results obtained show a slightly greater activation in monocytes and PMN cells isolated by one-step Ficoll-Hypaque procedure, in comparison with another method which uses both Ficoll-Hypaque 1077 and Ficoll-Hypaque 1119, although statistical differences were not significant.


Asunto(s)
Separación Celular/métodos , Fagocitos/citología , Animales , Centrifugación por Gradiente de Densidad , Diatrizoato , Ficoll , Técnicas In Vitro , Mediciones Luminiscentes , Ratones , Ratones Endogámicos BALB C , Monocitos/citología , Monocitos/fisiología , Neutrófilos/citología , Neutrófilos/fisiología , Fagocitos/fisiología , Fagocitosis
15.
J Antibiot (Tokyo) ; 50(6): 502-8, 1997 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-9268007

RESUMEN

The imipenem/cilastatin constitutes a broad spectrum beta-lactam antibiotic formulation, especially used in pre and post-operatory treatments for transplanted or drug-immunosuppresed patients. The effect of the dose and the duration of the treatment with imipenem/cilastatin on some parameters of natural immunity in BALB/c mice were examined. The treatment by intraperitoneal route with 1 or 2 g/70 kg/day during 7 days did not alter significantly the parameters tested, whereas the greater dose used (4 g/70 kg/day) had an inhibitory effect on peritoneal cell counts and phagocytic activity, as well as it caused an increase on IL-1 production and natural killer activity. The greater stimulating effect of innate immunity was obtained with the lowest imipenem/cilastatin dose used (0.5 g/70 kg/day). Since this antibiotic apparently does not impair the studied innate immune responses at 1 or 2 g/70 kg/day, it seems to be especially suited for the therapy of systemic bacterial infections in immunocompromised patients.


Asunto(s)
Antibacterianos/farmacología , Quimioterapia Combinada/farmacología , Inmunidad Innata/efectos de los fármacos , Animales , Recuento de Células/efectos de los fármacos , Cilastatina/farmacología , Combinación Cilastatina e Imipenem , Relación Dosis-Respuesta a Droga , Combinación de Medicamentos , Imipenem/farmacología , Interleucina-2/biosíntesis , Células Asesinas Naturales/efectos de los fármacos , Células Asesinas Naturales/inmunología , Leucocitos/efectos de los fármacos , Leucocitos/inmunología , Leucocitos/metabolismo , Macrófagos Peritoneales/citología , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/inmunología , Masculino , Ratones , Ratones Endogámicos BALB C , Cavidad Peritoneal/citología , Fagocitosis/efectos de los fármacos
17.
Microbiol Immunol ; 38(2): 89-95, 1994.
Artículo en Inglés | MEDLINE | ID: mdl-8041305

RESUMEN

After intraperitoneal inoculation with Campylobacter jejuni BALB/c, Swiss and DBA mice show a peritoneal inflammatory response of different intensity. Only BALB/c mice have a strong peritoneal response. Simultaneous intraperitoneal inoculation of C. jejuni plus FeCl3 increase both inflammatory response and phagocytic activity in Swiss mice, without production of diarrhea. Some thermostable compounds of C. jejuni have a very strong chemotactic activity against peritoneal cells of mice, whereas a diffusible, thermolabile and glutaraldehyde-resistant factor has an inhibitory effect over murine peritoneal cell phagocytosis. Bactericidal activity of peritoneal cells increased after in vitro re-challenge with C. jejuni. Bacteremia is present in all the mice strains tested, but the clearance is quick in DBA and slow in BALB/c and Swiss mice. These experiments confirm that in mice, peritoneal non-specific mechanisms of defense, such as macrophages, play an important role in order to control C. jejuni infection.


Asunto(s)
Infecciones por Campylobacter/inmunología , Campylobacter jejuni , Animales , Bacteriemia/etiología , Infecciones por Campylobacter/etiología , Infecciones por Campylobacter/prevención & control , Campylobacter jejuni/inmunología , Campylobacter jejuni/patogenicidad , Cloruros , Modelos Animales de Enfermedad , Compuestos Férricos/toxicidad , Inmunidad Celular , Macrófagos Peritoneales/inmunología , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos DBA , Peritonitis/etiología , Peritonitis/inmunología , Peritonitis/prevención & control , Fagocitosis , Especificidad de la Especie
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