RESUMEN

Treated silica xerogel with protic ionic liquid (PIL) and bifunctional agents (glutaraldehyde and epichlorohydrin) is a novel support strategy used in the effective immobilization of lipase from Burkholderia cepacia (LBC) by covalent binding. As biocatalysts with the highest activity recovery yields, LBC immobilized by covalent binding with epichlorohydrin without (203%) and with PIL (250%), was assessed by the following the hydrolysis reaction of olive oil and characterized biochemically (Michaelis⁻Menten constant, optimum pH and temperature, and operational stability). Further, the potential transesterification activity for three substrates: sunflower, soybean, and colza oils, was also determined, achieving a conversion of ethyl esters between 70 and 98%. The supports and the immobilized lipase systems were characterized using Fourier transform infrared spectra (FTIR), scanning electron microscopy (SEM), elemental analysis, and thermogravimetric (TG) analysis.

Asunto(s)

Proteínas Bacterianas/química , Enzimas Inmovilizadas/química , Líquidos Iónicos/química , Lipasa/química , Aceite de Oliva/química , Aceite de Soja/química , Aceite de Girasol/química , Proteínas Bacterianas/aislamiento & purificación , Biocombustibles/provisión & distribución , Burkholderia cepacia/química , Burkholderia cepacia/enzimología , Reactivos de Enlaces Cruzados/química , Enzimas Inmovilizadas/aislamiento & purificación , Epiclorhidrina/química , Esterificación , Geles , Glutaral/química , Humanos , Concentración de Iones de Hidrógeno , Lipasa/aislamiento & purificación , Dióxido de Silicio/química , Temperatura