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Lipase Immobilization on Silica Xerogel Treated with Protic Ionic Liquid and its Application in Biodiesel Production from Different Oils.
Carvalho, Nayára B; Vidal, Bruna T; Barbosa, Anderson S; Pereira, Matheus M; Mattedi, Silvana; Freitas, Lisiane Dos S; Lima, Álvaro S; Soares, Cleide M F.
Afiliación
  • Carvalho NB; Institute of Technology and Research, Avenida Murilo Dantas 300, Aracaju 49032-490, Sergipe, Brazil. nayara.eng@hotmail.com.
  • Vidal BT; Postgraduate in Process Engineering, Tiradentes University, Avenida Murilo Dantas 300, Aracaju 49032-490, Sergipe, Brazil. nayara.eng@hotmail.com.
  • Barbosa AS; Institute of Technology and Research, Avenida Murilo Dantas 300, Aracaju 49032-490, Sergipe, Brazil. brunabtv@hotmail.com.
  • Pereira MM; Institute of Technology and Research, Avenida Murilo Dantas 300, Aracaju 49032-490, Sergipe, Brazil. andinhobarbosa@hotmail.com.
  • Mattedi S; Institute of Technology and Research, Avenida Murilo Dantas 300, Aracaju 49032-490, Sergipe, Brazil. matheus.pereira@ua.pt.
  • Freitas LDS; Department of Chemical Engineering, Federal University of Bahia, Rua Prof. Aristides Novis, 02, Federação, Salvador 40210-630, Bahia, Brazil. silvana@ufba.br.
  • Lima ÁS; Department of Chemistry, Federal University of Sergipe, Av. Marechal Rondon, s/n, Jd. Rosa Elze, São Cristóvão 49100-000, Sergipe, Brazil. lisiane_santos_freitas@yahoo.com.br.
  • Soares CMF; Institute of Technology and Research, Avenida Murilo Dantas 300, Aracaju 49032-490, Sergipe, Brazil. aslima2001@yahoo.com.br.
Int J Mol Sci ; 19(7)2018 Jun 21.
Article en En | MEDLINE | ID: mdl-29933608
Treated silica xerogel with protic ionic liquid (PIL) and bifunctional agents (glutaraldehyde and epichlorohydrin) is a novel support strategy used in the effective immobilization of lipase from Burkholderia cepacia (LBC) by covalent binding. As biocatalysts with the highest activity recovery yields, LBC immobilized by covalent binding with epichlorohydrin without (203%) and with PIL (250%), was assessed by the following the hydrolysis reaction of olive oil and characterized biochemically (Michaelis⁻Menten constant, optimum pH and temperature, and operational stability). Further, the potential transesterification activity for three substrates: sunflower, soybean, and colza oils, was also determined, achieving a conversion of ethyl esters between 70 and 98%. The supports and the immobilized lipase systems were characterized using Fourier transform infrared spectra (FTIR), scanning electron microscopy (SEM), elemental analysis, and thermogravimetric (TG) analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Aceite de Soja / Enzimas Inmovilizadas / Líquidos Iónicos / Aceite de Oliva / Aceite de Girasol / Lipasa Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Aceite de Soja / Enzimas Inmovilizadas / Líquidos Iónicos / Aceite de Oliva / Aceite de Girasol / Lipasa Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza