RESUMEN
A latex agglutination test to detect urinary antigens for visceral leishmaniasis (VL) was studied. In 204 patients with suspected VL, KAtex had a sensitivity of 95.2% with good agreement with microscopy smears but poor agreement with 4 different serology tests. It was also positive in 2 confirmed VL cases co-infected with HIV. In all K4tex-positive confirmed cases actively followed up after treatment, the test became negative 1 month after completion of treatment. While IC4tex had a specificity of 100% in healthy endemic and non-endemic controls, the direct agglutination test (DAT) was positive in 14% of the KAtex-negative healthy endemic controls. KAtex is a simple addition to the diagnostics of VL particularly at field level and as a complementary test for the diagnosis of VL in smear-negative cases with positive DAT results.
Asunto(s)
Antígenos de Protozoos/orina , Pruebas de Fijación de Látex/métodos , Leishmania donovani/inmunología , Leishmaniasis Visceral/diagnóstico , Leishmaniasis Visceral/orina , Adolescente , Adulto , Animales , Estudios de Casos y Controles , Niño , Preescolar , Enfermedades Endémicas/estadística & datos numéricos , Ensayo de Inmunoadsorción Enzimática/normas , Femenino , Técnica del Anticuerpo Fluorescente Indirecta/normas , Humanos , Immunoblotting/normas , Lactante , Pruebas de Fijación de Látex/normas , Leishmaniasis Visceral/epidemiología , Leishmaniasis Visceral/inmunología , Masculino , Persona de Mediana Edad , Parasitología/normas , Pronóstico , Sensibilidad y Especificidad , Sudán/epidemiologíaRESUMEN
A latex agglutination test to detect urinary antigens for visceral leishmaniasis [VL] was studied. In 204 patients with suspected VL, KAtex had a sensitivity of 95.2% with good agreement with microscopy smears but poor agreement with 4 different serology tests. It was also positive in 2 confirmed VL cases co-infected with HIV. In all KAtex-positive confirmed cases actively followed up after treatment, the test became negative 1 month after completion of treatment. While KAtex had a specificity of 100% in healthy endemic and non-endemic controls, the direct agglutination test [DAT] was positive in 14% of the KAtex-negative healthy endemic controls. KAtex is a simple addition to the diagnostics of VL particularly at field level and as a complementary test for the diagnosis of VL in smear-negative cases with positive DAT results