RESUMEN
BACKGROUND: Radiotherapy is a commonly used treatment modality in head and neck cancer; however, it also negatively affects healthy structures. Direct damage to oral soft and hard tissue frequently occurs with radiotherapy. In this study, we aimed to evaluate the effect of radiotherapy on bone surrounding titanium dental implants via biomechanical and molecular methods. MATERIALS AND METHODS: Fifty-four implants were inserted in the left tibiae of 18 adult male New Zealand rabbits (3 implants in each rabbit). After 4 weeks of the implant surgery, the left tibiae of 12 rabbits were subjected to a single dose of irradiation (15 Gy or 30 Gy). Four weeks after the irradiation, rabbits were sacrificed and removal torque test was done for the biomechanical evaluation. Bone morphogenetic protein-2 (Bmp-2) and fibroblast growth factor-2 (Fgf-2) expression analyses were performed with Real-time PCR. Statistical analysis was done using SPSS. RESULTS: The control group showed significantly higher removal torque value than the 15 and 30 Gy irradiation groups, and the 15 Gy irradiation group had higher removal torque value than the 30 Gy irradiation group (p < .001). The 15 Gy and 30 Gy irradiation groups had significantly lower Bmp-2 and Fgf-2 mRNA expressions than the control group (p < .001). In addition, the 30 Gy irradiation group had significantly lower Bmp-2 (p < .01) and Fgf-2 mRNA expressions (p < .001) than the 15 Gy group. CONCLUSION: Radiotherapy with 15 and 30 Gy doses can adversely affect osseointegration of implants by reducing the quality of bone and impairing the bone-to-implant contact. The mechanism of action seems to be related to alterations in Bmp-2 and Fgf-2 mRNA expressions.
Asunto(s)
Proteína Morfogenética Ósea 2/efectos de la radiación , Implantes Dentales , Materiales Dentales/efectos de la radiación , Titanio/química , Animales , Materiales Dentales/química , Relación Dosis-Respuesta en la Radiación , Masculino , Oseointegración/efectos de la radiación , Conejos , Propiedades de Superficie , TorqueRESUMEN
Fetuin-A is a potent inhibitor of calcium-phosphate precipitation and of the calcification process, therefore it can also be related with dental calculus. Thus, we aimed to investigate a possible relationship between fetuin-A gene polymorphism and the presence of dental calculus. A possible relationship between serum, saliva and gingival crevicular fluid (GCF) levels of fetuin-A was also investigated. Fetuin-A c.742C > T and c.766C > G polymorphisms were investigated in 103 patients with or without dental calculus. Additionally, serum, saliva and GCF fetuin-A levels of patients were compared according to dental calculus presence. A significant difference was not observed in the distribution of the fetuin-A c.742C > T and c.766C > G polymorphisms between patients with or without dental calculus. Saliva and GCF fetuin-A concentrations of patients with dental calculus were statistically higher than those without dental calculus (P=0.001, P=0.036 respectively). According to our results, fetuin-A c.742C > T and c.766C > G polymorphisms were not associated with presence of dental calculus. However, higher GCF and saliva fetuin-A levels were detected in patients with dental calculus than in patients without dental calculus, which may result from an adaptive mechanism to inhibit mineral precipitation and eventually calculus formation.
Asunto(s)
Cálculos Dentales/química , Líquido del Surco Gingival/química , Polimorfismo Genético , Saliva/química , alfa-2-Glicoproteína-HS/análisis , alfa-2-Glicoproteína-HS/genética , Adulto , Análisis de Varianza , Estudios de Casos y Controles , Cálculos Dentales/genética , Cálculos Dentales/fisiopatología , Placa Dental/química , Ensayo de Inmunoadsorción Enzimática , Femenino , Estudios de Asociación Genética , Genotipo , Líquido del Surco Gingival/fisiología , Humanos , Masculino , Persona de Mediana Edad , Valores de Referencia , Saliva/fisiología , Estadísticas no Paramétricas , Adulto JovenRESUMEN
AIM: Matrix-Gla Protein (MGP) is one of the major Gla-containing protein associated with calcification process. It also has a high affinity for Ca2+ and hydroxyapatite. In this study we aimed to evaluate the MGP rs4236 [A/G] gene polymorphism in association with subgingival dental calculus. Also a possible relationship between MGP gene polymorphism and serum and GCF levels of MGP were examined. MATERIAL AND METHODS: MGP rs4236 [A/G] gene polymorphism was investigated in 110 patients with or without subgingival dental calculus, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. Additionally, serum and GCF levels of MGP of the patients were compared according to subgingival dental calculus. RESULTS: Comparison of patients with and without subgingival dental calculus showed no statistically significant difference in MGP rs4236 [A/G] gene polymorphism (p=0.368). MGP concentrations in GCF of patients with subgingival dental calculus were statistically higher than those without subgingival dental calculus (p=0.032). However, a significant association was not observed between the genotypes of AA, AG and GG of the MGP rs4236 gene and the serum and GCF concentrations of MGP in subjects. CONCLUSION: In this study, it was found that MGP rs4236 [A/G] gene polymorphism was not to be associated with subgingival dental calculus. Also, that GCF MGP levels were detected higher in patients with subgingival dental calculus than those without subgingival dental calculus independently of polymorphism, may be the effect of adaptive mechanism to inhibit calculus formation.
Asunto(s)
Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/metabolismo , Proteínas de la Matriz Extracelular/genética , Proteínas de la Matriz Extracelular/metabolismo , Líquido del Surco Gingival/metabolismo , Enfermedades Periodontales/metabolismo , Adulto , Proteínas de Unión al Calcio/sangre , Cálculos Dentales/sangre , Cálculos Dentales/genética , Cálculos Dentales/metabolismo , Proteínas de la Matriz Extracelular/sangre , Femenino , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Enfermedades Periodontales/sangre , Enfermedades Periodontales/genética , Reacción en Cadena de la Polimerasa/métodos , Polimorfismo Genético , Polimorfismo de Nucleótido Simple , Calcificación de Dientes , Proteína Gla de la MatrizRESUMEN
OBJECTIVE: The present study was aimed to compare the clinical and biochemical effectiveness of guided tissue regeneration (GTR) alone and combined with low-level laser therapy (LLLT) application in the treatment of furcation II periodontal defects, over a period of 6 months. MATERIAL AND METHODS: Thirty-three furcation defects were included in the study. Seventeen of these defects were treated with GTR plus LLLT, and sixteen of them were treated with GTR alone. Probing pocket depth (PPD), clinical attachment level (CAL), horizontal probing depth (HPD), and alkaline phosphatase (ALP) and osteocalcin (OC) levels in the gingival crevicular fluid (GCF) were recorded at baseline and at postoperative 3rd and 6th months. RESULTS: Healing was uneventful in all cases. At the 3rd and 6th months, both treatment modalities-GTR and GTR plus LLLT--showed improved PPD, CAL, and HPD values compared to their baseline values. ALP and OC levels in GCF increased after the treatment in both groups (p < 0.05). When compared the two groups, at the 6th month, PPD, CAL, HPD, and ALP values showed significantly more improvement in laser group than non-laser group (p < 0.05). CONCLUSIONS: The results of this study showed that both treatments led to significantly favorable clinical improvements in furcation periodontal defects. LLLT plus GTR may be a more effective treatment modality compared to GTR alone.
Asunto(s)
Defectos de Furcación/terapia , Regeneración Tisular Dirigida/métodos , Terapia por Luz de Baja Intensidad/métodos , Cicatrización de Heridas , Adulto , Fosfatasa Alcalina/análisis , Terapia Combinada , Femenino , Defectos de Furcación/radioterapia , Líquido del Surco Gingival/química , Humanos , Masculino , Persona de Mediana Edad , Osteocalcina/análisis , Estudios ProspectivosRESUMEN
PURPOSE: To evaluate the association between cytokines IL-6 and IL-10 in saliva, obesity and periodontal disease. METHODS: The study was carried out in 40 subjects: 20 obese subjects with gingivitis and 20 non-obese subjects with gingivitis (controls). Periodontal parameters such as gingival index (GI) and plaque index (PI) of subjects were recorded. Saliva samples were used for measuring IL-6 and IL-10 levels by ELISA method. RESULTS: Saliva IL-6 levels were significantly higher in obese subjects than those of non-obese subjects (P= 0.002). When total groups were evaluated, negative significant correlation between GI and salivary IL-10 levels (r= -0.452, P= 0.003) and positive correlations between salivary IL-6 level and body mass index (BMI) (r= 0.369, P= 0.019) were found. There was a negative correlation between the GI and salivary IL-10 levels in obese subjects (r= -0.548, P= 0.012). Also there was a positive correlation between the salivary level of IL-6 and IL-10 in obese subjects (r= 0.594, P= 0.006). CLINICAL SIGNIFICANCE: Obesity and adipose tissues can affect periodontal health by altering the levels of IL-6 and IL-10. IL-6 in saliva clearly increased in subjects with obesity compared to subjects without obesity. Also negative correlations between saliva IL-10 levels and GI were found in the total group and obese subjects.
Asunto(s)
Gingivitis/metabolismo , Interleucina-10/metabolismo , Interleucina-6/metabolismo , Obesidad/metabolismo , Saliva/química , Adulto , Estudios de Casos y Controles , Índice de Placa Dental , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Masculino , Persona de Mediana Edad , Índice PeriodontalRESUMEN
ABSTRACT: Fetuin-A is a potent inhibitor of calcium-phosphate precipitation and of the calcification process, therefore it can also be related with dental calculus. Thus, we aimed to investigate a possible relationship between fetuin-A gene polymorphism and the presence of dental calculus. A possible relationship between serum, saliva and gingival crevicular fluid (GCF) levels of fetuin-A was also investigated. Fetuin-A c.742C > T and c.766C > G polymorphisms were investigated in 103 patients with or without dental calculus. Additionally, serum, saliva and GCF fetuin-A levels of patients were compared according to dental calculus presence. A significant difference was not observed in the distribution of the fetuin-A c.742C > T and c.766C > G polymorphisms between patients with or without dental calculus. Saliva and GCF fetuin-A concentrations of patients with dental calculus were statistically higher than those without dental calculus (P=0.001, P=0.036 respectively). According to our results, fetuin-A c.742C > T and c.766C > G polymorphisms were not associated with presence of dental calculus. However, higher GCF and saliva fetuin-A levels were detected in patients with dental calculus than in patients without dental calculus, which may result from an adaptive mechanism to inhibit mineral precipitation and eventually calculus formation.
Asunto(s)
Humanos , Masculino , Femenino , Adulto , Adulto Joven , Polimorfismo Genético , Saliva/química , Cálculos Dentales/química , Líquido del Surco Gingival/química , alfa-2-Glicoproteína-HS/análisis , alfa-2-Glicoproteína-HS/genética , Valores de Referencia , Saliva/fisiología , Ensayo de Inmunoadsorción Enzimática , Cálculos Dentales/fisiopatología , Cálculos Dentales/genética , Estudios de Casos y Controles , Análisis de Varianza , Líquido del Surco Gingival/fisiología , Estadísticas no Paramétricas , Placa Dental/química , Estudios de Asociación Genética , Genotipo , Persona de Mediana EdadRESUMEN
OBJECTIVE: The purpose of the present study was to evaluate the clinical results of guided tissue regeneration (GTR) after the application of equine bone and membrane alone or combined with low-level laser therapy (LLLT) for the treatment of periodontal defects. MATERIALS AND METHODS: This study was an intra-individual longitudinal study of 6 months' duration conducted using a split-mouth and randomized design. In 13 periodontitis patients with bilateral intrabony periodontal defects, while one defect site was treated with GTR plus LLLT (1064 nm, 100 mW, with energy density of 4 J/cm(2)), the contralateral defect site was treated with guided GTR alone. GTR was performed with a combination of equine bone and membrane. LLLT was used both intra- and postoperatively. Clinical probing depth (PPD), clinical attachment level (CAL), clinical gingival recession level (REC), plaque index (PI) score, and sulcus blooding index (SBI) score were recorded at the time of surgery, and at the 3rd and 6th months after operation. RESULTS: The treatment of periodontal intrabony defects with equine bone and membrane in the operation of GTR alone or GTR plus LLLT in combination led to statistically significant PPD reduction, CAL gain, and lower SBI score at the end of the study (p<0.05). In addition, between the two groups, GTR plus LLLT resulted in statistically significant lower REC (p=0.025), lower SBI (p=0.008) score, more reduction of PPD (p=0.009) and CAL gain (p=0.002) compared with GTR alone at 6th month control. CONCLUSIONS: This study showed that GTR is an effective treatment for periodontal regeneration, and that LLLT may improve the effects of GTR in the treatment of periodontal defects.