RESUMEN

Escherichia coli (E. coli) that expresses galactose-reactive lectins, like K88 adhesin, causes high mortality among piglets. Carbohydrates that compete for adhesion could serve as an alternative for disease prevention. Porcine serum albumin (PSA) was modified by non-enzymatic glycation with lactose to produce PSA-Lac or PSA-Glc beta (1-4) Gal, as confirmed by reduction of available free amino groups, increased molecular mass and by Ricinus communis lectin recognition. E. coli K88 binds to PSA-Lac treatments containing three and four lactoses, respectively. In addition, PSA-Lac partially inhibited K88 strain adherence to mucins. These results suggest that neoglycoconjugates obtained by non-enzymatic glycation of proteins may serve in the prophylaxis of piglets' diarrhea.

Asunto(s)

Adhesinas de Escherichia coli/metabolismo , Antígenos Bacterianos/metabolismo , Proteínas de Escherichia coli/metabolismo , Proteínas Fimbrias/metabolismo , Albúmina Sérica/metabolismo , Adhesinas de Escherichia coli/química , Aminas , Animales , Antígenos Bacterianos/química , Adhesión Bacteriana/efectos de los fármacos , Electroforesis , Escherichia coli/citología , Escherichia coli/efectos de los fármacos , Escherichia coli/metabolismo , Proteínas de Escherichia coli/química , Proteínas Fimbrias/química , Glicosilación/efectos de los fármacos , Lactosa/metabolismo , Peso Molecular , Lectinas de Plantas/farmacología , Albúmina Sérica/química , Albúmina Sérica/aislamiento & purificación , Albúmina Sérica/farmacología , Espectrometría de Fluorescencia , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Sus scrofa , Triptófano/metabolismo