RESUMEN
STUDY DESIGN: This is a clinical trial (phase 1). OBJECTIVES: The objective of this study was to asses the safety and feasibility of bone marrow mesenchymal stem cell (MSC) and Schwann cell (SC) co-injection through cerebral spinal fluid (CSF) for the treatment of patients with chronic spinal cord injury. METHODS: Six subjects with complete spinal cord injury due to trauma according to International Standard of Neurological Classification for Spinal Cord Injury (ISNCSCI) developed by the American Spinal Injury Association were enrolled. They received autologous co-transplantation of MSC and SC through lumbar puncture. Neurological status of the patients was determined by ISNCSCI, as well as by assessment of functional status by Spinal Cord Independent Measure. Before and after cell transplantation, magnetic resonance imaging (MRI) was performed for all the patients. Before the procedure, all the patients underwent electromyography, urodynamic study (UDS) and MRI tractograghy. After transplantation, these assessments were performed in special cases when the patients reported any changes in motor function or any changes in urinary sensation. RESULTS: Over the mean 30 months of follow-up, the radiological findings were unchanged without any evidence of neoplastic tissue overgrowth. American Spinal Injury Association class in one patient was changed from A to B, in addition to the improvement in indexes of UDS, especially bladder compliance, which was congruous with axonal regeneration detected in MRI tractography. No motor score improvement was observed among the patients. CONCLUSION: No adverse findings were detected at a mean of 30 months after autologous transplantation of the combination of MSCs and SCs through CSF. It may suggest the safety of this combination of cells for spinal cord regeneration.
Asunto(s)
Trasplante de Células Madre Mesenquimatosas/efectos adversos , Células de Schwann/trasplante , Traumatismos de la Médula Espinal/líquido cefalorraquídeo , Traumatismos de la Médula Espinal/terapia , Regeneración de la Medula Espinal , Adulto , Líquido Cefalorraquídeo/citología , Enfermedad Crónica , Terapia Combinada/efectos adversos , Terapia Combinada/métodos , Estudios de Factibilidad , Femenino , Humanos , Masculino , Trasplante de Células Madre Mesenquimatosas/métodos , Persona de Mediana Edad , Células de Schwann/citología , Traumatismos de la Médula Espinal/diagnóstico , Trasplante Autólogo/efectos adversos , Trasplante Autólogo/métodos , Resultado del TratamientoRESUMEN
In many texts, both theoretical and experimental studies on molecular structure and spectroscopic assignments of anticancer medicines have been reported. Molecular geometry parameters have been experimentally obtained by x-ray structure determination method and optimized using computational chemistry method like density functional theory. In this review, we consider calculations based on density function theory at B3LYP/6-31G (d,p) and B3LYP/6-311++G (d,p) levels of theory. Based on optimized geometric parameters of the molecules, molecular structures (length of bonds, bond angles and torsion angles) and vibrational assignments have been obtained. Molecular stability and bond strength have been investigated by applying natural bond orbital (NBO) analysis. Other molecular properties such as mulliken population analysis, thermodynamic properties and polarizabitities of these drugs have been reported. Calculated energies of HOMO and LUMO show that charge transfer occurs in the molecular. Information about the size, shape, charge density distribution and site of molecular chemical reactivity has been obtained by mapping electron density isosurface of electrostatic and compared with experiment data.
Asunto(s)
Antineoplásicos/química , Modelos Químicos , Enlace de Hidrógeno , Modelos Moleculares , Estructura Molecular , Teoría Cuántica , Espectroscopía Infrarroja por Transformada de Fourier , Espectrometría Raman , Electricidad Estática , Termodinámica , VibraciónRESUMEN
BACKGROUND: NLRP7 (NALP7) has recently been identified as the causative gene for familial recurrent hydatidiform mole (FRHM), a rare autosomal recessive condition in which affected women have recurrent molar pregnancies of diploid biparental origin. To date only a small number of affected families have been described. Our objectives were to investigate the diversity of mutations and their localisation to one or both isoforms of NLRP7, by screening a large series of women with FRHM and to examine the normal expression of NLRP7 in ovarian tissue. METHODS: Fluorescent microsatellite genotyping of molar tissue was used to establish a diagnosis of FRHM. Twenty families were subsequently screened for mutations in NLRP7 using DNA sequencing. Expression of NLRP7 in the ovary was examined by immunohistochemical staining. RESULTS: 16 different mutations were identified in the study, 13 of which were novel. Missense mutations were found to be present in transcript variant 2 of NLRP7 and cluster in the leucine-rich region (LRR). A man with two affected sisters and homozygous for the p.R693P mutation had normal reproductive outcomes. In the normal human ovary, NLRP7 expression is confined to the oocytes and present at all stages from primordial to tertiary follicles. CONCLUSION: 13 novel mutations in NLRP7 were identified. We confirm that mutations in NLRP7 affect female but not male reproduction, and provide evidence that transcript variant 2 of NLRP7 is the important isoform in this condition. The mutation clustering seen confirms that the LRR is critical for normal functioning of NLRP7.
Asunto(s)
Proteínas Adaptadoras Transductoras de Señales/genética , Mola Hidatiforme/genética , Mutación Missense , Proteínas Adaptadoras Transductoras de Señales/metabolismo , Adulto , Familia , Femenino , Histocitoquímica , Humanos , Leucina , Masculino , Repeticiones de Microsatélite , Ovario/metabolismo , Polimorfismo de Nucleótido Simple , Embarazo , RecurrenciaRESUMEN
ABSTRACT This study investigated whether breast arterial calcification (BAC) has an association with coronary artery diseases (CAD) in young premenopausal women and evaluated the association of BAC with carotid intima-media thickness and standard CAD risk factors. Among 84 premenopausal women aged < 55 years who were referred for coronary angiography, 34 (40.5%) had abnormal angiographic findings and 6 (7.1%) showed BAC in their mammograms. The body mass index of patients with BAC was significantly higher than those without BAC. BAC had no significant association with angiography-confirmed CAD.
Asunto(s)
Calcinosis/epidemiología , Enfermedades de las Arterias Carótidas/epidemiología , Enfermedad de la Arteria Coronaria/epidemiología , Arterias Mamarias , Premenopausia , Túnica Media/patología , Adulto , Índice de Masa Corporal , Calcinosis/complicaciones , Calcinosis/diagnóstico por imagen , Enfermedades de las Arterias Carótidas/complicaciones , Enfermedades de las Arterias Carótidas/diagnóstico por imagen , Distribución de Chi-Cuadrado , Angiografía Coronaria , Enfermedad de la Arteria Coronaria/complicaciones , Femenino , Humanos , Irán/epidemiología , Modelos Logísticos , Mamografía , Tamizaje Masivo , Persona de Mediana Edad , Prevalencia , Factores de Riesgo , Estadísticas no Paramétricas , Ultrasonografía DopplerRESUMEN
This study investigated whether breast arterial calcification [BAC] has an association with coronary artery diseases [CAD] in young premenopausal women and evaluated the association of BAC with carotid intima-media thickness and standard CAD risk factors. Among 84 premenopausal women aged < 55 years who were referred for coronary angiography, 34 [40.5%] had abnormal angiographic findings and 6 [7.1%] showed BAC in their mammograms. The body mass index of patients with BAC was significantly higher than those without BAC. BAC had no significant association with angiography-confirmed CAD
Asunto(s)
Arterias Carótidas , Calcinosis , Factores de Riesgo , Premenopausia , Angiografía Coronaria , Índice de Masa Corporal , Mamografía , Mama , Encuestas y Cuestionarios , Enfermedad CoronariaRESUMEN
BACKGROUND: Polyglandular Autoimmune syndromes (PGAs) or polyendocrinopathies are immune mediated multiple endocrine gland failure sometimes accompanied by nonendocrine autoimmune disorders with varieties of presentations. CASE REPORT: We describe a case of a middle aged man with severe cognitive dysfunction, brain atrophy, adrenal insufficiency, hypothyroidism, renal failure, thrombocytopenia, and positive antiphospholipid antibodies, with significant renal and cognitive improvement after hormone replacement. CONCLUSIONS: PGAs may present with a broad spectrum of manifestations related to different organs like nervous,renal,cardiac and hematopoietic systems, sometimes challenging both to physician and the patient.