RESUMEN

OBJECTIVE: To determine and compare the immunolocalization of functionally important antigens in human spermatozoa in an unexplained infertility (UI) group. MATERIALS AND METHODS: In this study, the sperm samples of 20 patients undergoing evaluation belonging to normozoospermic group, whose primary reason of infertility was under investigation for this purpose, were screened. CD46, CD55 and CD52, CD69, CD98, fMLP, HI307, and 80280 were stained on the spermatozoa through indirect immunofluorescence technique. RESULTS: In addition to CD46, CD55, and CD52 antigens, which are known to be localized on human spermatozoa, significant immunolocalization of several novel antigens including: CD52, CD69, CD98, fMLP, HI307, and 80280 were determined on the spermatozoa of the unexplained infertility group, possibly reflecting important roles in the pathophysiology of such unresolved clinical situations. CONCLUSION: Identification and characterization of antigens present on sperm cells is crucial for understanding of the diagnosis and treatment of unexplained infertility. Further studies were conducted to evaluate a possible correlation between the expression of these antigens and clinical outcomes in different well-defined infertility groups.

Asunto(s)

Antígenos/análisis , Infertilidad/inmunología , Espermatozoides/inmunología , Antígenos/inmunología , Antígenos CD/análisis , Antígenos de Diferenciación de Linfocitos T/análisis , Antígenos de Neoplasias/análisis , Antígeno CD52 , Antígeno CD56/análisis , Femenino , Técnica del Anticuerpo Fluorescente Indirecta , Proteína-1 Reguladora de Fusión/análisis , Glicoproteínas/análisis , Humanos , Lectinas Tipo C/análisis , Masculino , Proteína Cofactora de Membrana/análisis , Receptores de Formil Péptido/análisis

RESUMEN

Pharmacobehavioral studies in experimental animals, and imaging studies in humans, indicate that serotonergic transmission in the amygdala plays a key role in emotional processing, especially for anxiety-related stimuli. The lateral and basolateral amygdaloid nuclei receive a dense serotonergic innervation in all species studied to date. We investigated interrelations between serotonergic afferents and neuropeptide Y (NPY)-producing neurons, which are a subpopulation of inhibitory interneurons in the rat lateral and basolateral nuclei with particularly strong anxiolytic properties. Dual light microscopic immunolabeling showed numerous appositions of serotonergic afferents on NPY-immunoreactive somata. Using electron microscopy, direct membrane appositions and synaptic contacts between serotonin-containing axon terminals and NPY-immunoreactive cellular profiles were unequivocally established. Double in situ hybridization documented that more than 50 %, and about 30-40 % of NPY mRNA-producing neurons, co-expressed inhibitory 5-HT1A and excitatory 5-HT2C mRNA receptor subtype mRNA, respectively, in both nuclei with no gender differences. Triple in situ hybridization showed that individual NPY mRNA-producing interneurons co-express both 5-HT1A and 5-HT2C mRNAs. Co-expression of NPY and 5-HT3 mRNA was not observed. The results demonstrate that serotonergic afferents provide substantial innervation of NPY-producing neurons in the rat lateral and basolateral amygdaloid nuclei. Studies of serotonin receptor subtype co-expression indicate a differential impact of the serotonergic innervation on this small, but important, population of anxiolytic interneurons, and provide the basis for future studies of the circuitry underlying serotonergic modulation of emotional stimulus processing in the amygdala.

Asunto(s)

Amígdala del Cerebelo/metabolismo , Interneuronas/metabolismo , Neuropéptido Y/metabolismo , Receptores de Serotonina/metabolismo , Neuronas Serotoninérgicas/metabolismo , Amígdala del Cerebelo/citología , Animales , Conducta Animal , Femenino , Inmunohistoquímica , Hibridación in Situ , Interneuronas/ultraestructura , Masculino , Microscopía Electrónica , Neuropéptido Y/genética , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Receptor de Serotonina 5-HT1A/metabolismo , Receptor de Serotonina 5-HT2C/metabolismo , Receptores de Serotonina/genética , Receptores de Serotonina 5-HT3/metabolismo , Neuronas Serotoninérgicas/ultraestructura , Serotonina/metabolismo , Proteínas de Transporte de Serotonina en la Membrana Plasmática/metabolismo

RESUMEN

OBJECTIVE: Intra-articular local anesthetics are often used for prevention of pain after arthroscopic knee surgery. However, the effect of local anesthetics other than bupivacaine on articular cartilage and synovium has not been studied. Also, complications associated with the injection of intra-articular bupivacaine have appeared in the literature. The aim of this study was to evaluate the effects of levobupivacaine on the articular cartilage and the synovium in rats. METHODS: Under aseptic conditions 0.25 ml (5 mg/ml) of levobupivacaine was injected into the right knee joint while 0.25 ml of saline was simultaneously injected into the left knee joint of 20 adult Sprague-Dawley rats. The purpose of saline injections was to serve as a control group. Groups of five rats were killed on days 1, 7, 14 and 21 after administration of injections. The knee joint samples were evaluated for the presence of inflammation in the articular and periarticular tissues and the synovium. RESULTS: There were no significant differences between the levobupivacaine and control groups with respect to inflammation in the articular and periarticular tissues and the synovium. CONCLUSIONS: Although more studies are needed before final recommendations can be made, by evaluating the results obtained from this study, the clinical use of intra-articular levobupivacaine can be recommended for arthroscopic knee surgery.

Asunto(s)

Anestésicos Locales/administración & dosificación , Anestésicos Locales/toxicidad , Cartílago Articular/efectos de los fármacos , Membrana Sinovial/efectos de los fármacos , Animales , Bupivacaína/administración & dosificación , Bupivacaína/análogos & derivados , Bupivacaína/toxicidad , Cartílago Articular/patología , Inflamación/inducido químicamente , Inflamación/patología , Inyecciones Intraarticulares , Articulaciones/patología , Levobupivacaína , Ratas , Ratas Sprague-Dawley , Membrana Sinovial/patología

RESUMEN

Low expression of the human serotonin transporter (5-HTT) gene presumably interacts with stressful life events enhancing susceptibility for affective disorders. 5-Htt knockout (KO) mice display an anxious phenotype, and behavioural differences compared to wild-type (WT) mice are exacerbated after repeated loser experience in a resident-intruder stress paradigm. To assess whether genotype-dependent and stress-induced behavioural differences are reflected in alterations of neuronal morphology in limbic areas, we studied dendritic length and complexity of pyramidal neurons in the anterior cingulate and infralimbic cortices (CG, IL), hippocampus CA1 region, and of pyramidal neurons and interneurons in the lateral (La) and basolateral (BL) amygdaloid nuclei in Golgi-Cox-stained brains of male WT and 5-Htt KO control and loser mice. Spine density was analysed for IL apical and amygdaloid apical and basal pyramidal neuron dendrites. While group differences were absent for parameters analysed in CG, CA1 and amygdaloid interneurons, pyramidal neurons in the IL displayed tendencies to shorter and less spinous distal apical dendrites in 5-Htt KO controls, and to extended proximal dendrites in WT losers compared to WT controls. In contrast, spine density of several dendritic compartments of amygdaloid pyramids was significantly higher in 5-Htt KO mice compared to WT controls. While a tendency to increased spine density was observed in the same dendritic compartments in WT after stress, changes were lacking in stressed compared to control 5-Htt KO mice. Our findings indicate that disturbed 5-HT homeostasis results in alterations of limbic neuronal morphology, especially in higher spinogenesis in amygdaloid pyramidal neurons. Social stress leads to similar but less pronounced changes in the WT, and neuroplasticity upon stress is reduced in 5-Htt KO mice.

Asunto(s)

Sistema Límbico/patología , Plasticidad Neuronal/fisiología , Neuronas/patología , Proteínas de Transporte de Serotonina en la Membrana Plasmática/deficiencia , Estrés Psicológico/genética , Estrés Psicológico/patología , Animales , Dendritas/patología , Dendritas/ultraestructura , Espinas Dendríticas/patología , Espinas Dendríticas/ultraestructura , Modelos Animales de Enfermedad , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Neuronas/clasificación , Neuronas/ultraestructura , Proteínas de Transporte de Serotonina en la Membrana Plasmática/genética , Tinción con Nitrato de Plata

RESUMEN

Our aim in this study was to compare reflection contrast microscopy (RCM) with transmission electron microscopy (TEM) to understand whether RCM could be used in the histopathological diagnosis of various kidney diseases as a less expensive and an easier alternative to TEM. The diagnoses of kidney pathologic lesions included Alport syndrome, thin membrane disease, Ig A nephropathy. RCM is a form of light microscope that works in the reflected mode, suitable to observe ultrathin (50-100 nm) plastic sections that is also used in TEM. Our findings showed that RCM showed similar results compared with TEM on these lesions described earlier.

Asunto(s)

Enfermedades Renales/diagnóstico , Microscopía Electrónica de Transmisión/métodos , Microscopía de Contraste de Fase/métodos , Glomerulonefritis por IGA/diagnóstico , Humanos , Riñón/patología , Riñón/ultraestructura , Túbulos Renales/patología , Túbulos Renales/ultraestructura , Nefritis Hereditaria/diagnóstico , Reproducibilidad de los Resultados , Sensibilidad y Especificidad

RESUMEN

Current surgical treatment of spinal root injuries aims at reconnecting ventral roots to the spinal cord while severed dorsal roots are generally left untreated. Reactive changes in dorsal root ganglia (DRGs) and in injured dorsal roots after such complex lesions have not been analysed in detail. We studied dorsal root remnants and lesioned DRGs 6 months after C7 dorsal rhizotomy, ventral root avulsion and immediate ventral root replantation in adult rabbits. Replanted ventral roots were fixed to the spinal cord with fibrin glue only or with glue containing ciliary neurotrophic factor and/or brain-derived neurotrophic factor. Varying degrees of degeneration were observed in the deafferented dorsal spinal cord in all experimental groups. In cases with well-preserved morphology, small myelinated axons extended into central tissue protrusions at the dorsal root entry zone, suggesting sprouting of spinal neuron processes into the central dorsal root remnant. In lesioned DRGs, the density of neurons and myelinated axons was not significantly altered, but a slight decrease in the relative frequency of large neurons and an increase of small myelinated axons was noted (significant for axons). Unexpectedly, differences in the degree of these changes were found between control and neurotrophic factor-treated animals. Central axons of DRG neurons formed dorsal root stumps of considerable length which were attached to fibrous tissue surrounding the replanted ventral root. In cases where gaps were apparent in dorsal root sheaths, a subgroup of dorsal root axons entered this fibrous tissue. Continuity of sensory axons with the spinal cord was never observed. Some axons coursed ventrally in the direction of the spinal nerve. Although the animal model does not fully represent the situation in human plexus injuries, the present findings provide a basis for devising further experimental approaches in the treatment of combined motor/sensory root lesions.

Asunto(s)

Ganglios Espinales/patología , Raíces Nerviosas Espinales/lesiones , Raíces Nerviosas Espinales/patología , Animales , Axones/patología , Femenino , Ganglios Espinales/cirugía , Procesamiento de Imagen Asistido por Computador , Microscopía Fluorescente , Modelos Animales , Neuronas/patología , Periodo Posoperatorio , Conejos , Reimplantación/métodos , Rizotomía , Raíces Nerviosas Espinales/cirugía , Coloración y Etiquetado

RESUMEN

Ciliary neurotrophic factor (CNTF) has been implicated in processes of neuroprotection, axonal regeneration and synaptogenesis in the lesioned CNS. In the olfactory system, which is characterized by particularly robust neuroplasticity throughout life, the concentration of CNTF is high even under physiological conditions. In the present study, the cellular localization of CNTF-immunoreactivity was studied in the rat and mouse olfactory epithelium. In both species, individual olfactory sensory neurons (ONs) displayed intense CNTF-immunoreactivity. The number of CNTF-ir ONs varied interindividually in rats and was lower in mice than in rats. In olfactory epithelia of mice expressing beta-galactosidase under control of the CNTF promoter, cells of the ON layer were immunoreactive for the reporter protein. CNTF-ir ONs were olfactory marker protein-positive and growth associated protein 43-negative. CNTF-ir ONs lacked apoptotic markers, and the number of specifically labeled ONs was apparently unchanged after light chemical lesioning of the epithelium, indicating that CNTF-immunoreactivity was not associated with ON death. Electron microscopy of CNTF-ir ON axons in innervated olfactory bulb glomeruli documented that they formed typical ON axonal synapses with target neurons. Three dimensional reconstructions of bulb pairs showed a striking similarity of the positions of glomeruli innervated by CNTF-ir ON axons in left and right bulbs of individual animals and interindividually. The number of innervated glomeruli differed interindividually in rats and was lower in mice than in rats. The results show that in rodents CNTF-immunoreactivity occurs in a subset of mature, functionally competent ONs. The localization of target glomeruli suggests that CNTF-immunoreactivity may be associated with the expression and/or activation of specific olfactory receptor proteins.

Asunto(s)

Factor Neurotrófico Ciliar/metabolismo , Bulbo Olfatorio/citología , Vías Olfatorias/citología , Neuronas Receptoras Olfatorias/citología , Neuronas Receptoras Olfatorias/metabolismo , Animales , Apoptosis/fisiología , Femenino , Imagenología Tridimensional , Inmunohistoquímica , Etiquetado Corte-Fin in Situ , Masculino , Ratones , Ratones Noqueados , Microscopía Electrónica de Transmisión , Bulbo Olfatorio/patología , Vías Olfatorias/metabolismo , Vías Olfatorias/patología , Neuronas Receptoras Olfatorias/ultraestructura , Ratas , Ratas Wistar

RESUMEN

In order to determine the effect of nerve root replantation on motoneuron survival and regeneration, we have avulsed and replanted C7 ventral rootlets in adult rabbits under various conditions. Intraspinal alterations and exact positions of ventrolateral replantations were studied in each animal, and the effects of BDNF and/or CNTF administration during replantation investigated in different experimental groups. Six months after lesion, about 70% of motoneurons were lost on the lesioned sides in the C7 segment, without significant differences between groups. Retrograde fluorescent tracing and histological analysis documented that many axons had regrown through the original ventral exit zones or had exited the spinal cord at the lateral replantation site. However, many laterally exiting axons had not grown out directly from the ventral horn through the lateral white matter but had elongated vertically before leaving the spinal cord. The mean axonal diameter was significantly higher in regenerated axons that had exited through the original ventral exit zones in comparison with axons which had grown out laterally. Application of BDNF and/or CNTF did not show any effects on the pathways of regeneration into the replanted root. The results indicate that motoneuron survival cannot be significantly improved by a single dose of neurotrophic factors applied to a ventrolateral replantation site. However, a significant number of myelinating axons are found in replanted roots, and regeneration may be more efficient when outgrowth through the original ventral exit zone is supported.

Asunto(s)

Neuronas Motoras/fisiología , Factores de Crecimiento Nervioso/farmacología , Regeneración Nerviosa/fisiología , Radiculopatía/terapia , Reimplantación/métodos , Raíces Nerviosas Espinales/fisiología , Animales , Factor Neurotrófico Derivado del Encéfalo/farmacología , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/fisiología , Factor Neurotrófico Ciliar/farmacología , Desnervación , Modelos Animales de Enfermedad , Femenino , Conos de Crecimiento/efectos de los fármacos , Conos de Crecimiento/fisiología , Neuronas Motoras/efectos de los fármacos , Factores de Crecimiento Nervioso/uso terapéutico , Regeneración Nerviosa/efectos de los fármacos , Procedimientos Neuroquirúrgicos , Conejos , Radiculopatía/fisiopatología , Médula Espinal/efectos de los fármacos , Médula Espinal/fisiología , Médula Espinal/cirugía , Raíces Nerviosas Espinales/efectos de los fármacos , Raíces Nerviosas Espinales/cirugía , Resultado del Tratamiento

RESUMEN

Microvillar cells (MCs) have been identified in the olfactory epithelium of various mammalian species from rodents to humans. Studies on properties and functions of MCs to date have yielded partially controversial results, supporting alternatively an epithelial or a neuronal nature of these cells. In the present study, single and double immunolabeling investigations were carried out using antibodies against cytoskeletal and integral membrane proteins in order to further characterize MCs in rat and mouse olfactory epithelium. Application of antibodies against ankyrin (ANK), a protein that links integral membrane proteins to the submembrane cytoskeleton, led to intense labeling of the basolateral membranes of numerous cells with characteristic MC morphology. ANK-immunoreactive (ir) cells bore an apical tuft of beta-actin-ir microvilli, were filled with cytokeratin 18 (CK18)-ir filamentous network, and extended a basal process that appeared to end above the basal membrane. Immunoreactions for villin, an actin-crosslinking protein particularly prominently expressed in brush cells in the gastrointestinal and respiratory tract epithelia, and for the alpha-subunit of sodium-potassium ATPase (Na(+), K(+)-ATPase), revealed that ANK-ir MCs fall into two subpopulations. The less frequent type I MCs displayed villin immunoreactivity in their apical microvilli and underneath the basolateral membranes; the more numerous type II MCs were negative for villin but possessed intense basolateral immunoreactivity for Na(+), K(+)-ATPase. Strong reactivity for the epithelial-type integral membrane protein of adherens junctions, E-Cadherin, was localized in basolateral membranes of both types of MCs. Our results support an epithelial nature of ANK-ir MCs in rat and mouse olfactory epithelium. Type I MCs strongly resemble brush cells in their immunocytochemical characteristics, namely, their ANK reactivity, CK18 reactivity, and villin reactivity. The intense Na(+), K(+)-ATPase reactivity of type II MCs implicates these cells in transport processes.

Asunto(s)

Ancirinas/análisis , Mucosa Olfatoria/química , Animales , Femenino , Inmunohistoquímica , Queratinas/análisis , Masculino , Ratones , Ratones Endogámicos C57BL , Proteínas de Microfilamentos/análisis , Microvellosidades/química , Mucosa Olfatoria/citología , Mucosa Olfatoria/ultraestructura , Ratas , Ratas Wistar , ATPasa Intercambiadora de Sodio-Potasio/análisis

RESUMEN

Corticotropin-releasing-factor (CRF) containing systems and monoaminergic afferents of the central amygdaloid nucleus (Ce) are crucial players in central nervous stress responses. For functional analyses of specific roles of these systems, numerous mouse models have been generated which lack or overexpress individual signal transduction components. Since data concerning system morphologies in murine brain are rarely available, mouse studies are usually designed and interpreted based on previous findings in rats, although interspecies differences are frequent. In the present study, in situ hybridization for CRF mRNA and correlative immunocytochemistry for CRF and monoaminergic afferents revealed numerous CRF mRNA-reactive neurons in the lateral Ce subnucleus (CeL) codistributed with dense dopaminergic fiber plexus in mice as has been demonstrated in rats. However, while in rats the lateral capsular Ce (CeLc) displays only scarce CRF immunoreactive (CRF-ir) innervation, particularly dense CRF-ir fiber plexus were observed in the CeLc in mice, with differences in labeling densities between different strains. CRF-ir terminal fibers overlap with the moderate serotonergic innervation of this subnucleus in mice. Additionally, CRF mRNA-reactive neurons were found immediately dorsal to the amygdala in the region of the interstitial nucleus of the posterior limb of the anterior commissure/amygdalostriatal transition area in both species. In mice, this region displayed dense CRF-ir fiber plexus, with variations between the strains. The results indicate that in mice and rats dopaminergic afferents represent the primary monoaminergic input to the CRF neurons in the CeL. In mice only, CRF-ir afferents provide dense innervation of CeLc neurons. Since the CeLc lacks dopaminergic input in both species but possesses moderate serotonergic afferents, CRF/serotonin interactions may occur selectively in mouse CeLc. The observed interspecies and interstrain differences in CRF input and CRF/monoaminergic interactions may influence the interpretation of findings concerning Ce functions in stress and fear in mouse models.

Asunto(s)

Amígdala del Cerebelo/metabolismo , Monoaminas Biogénicas/metabolismo , Hormona Liberadora de Corticotropina/metabolismo , Neuronas Aferentes/metabolismo , Amígdala del Cerebelo/citología , Animales , Inmunohistoquímica , Hibridación in Situ , Ratones , Ratones Endogámicos , Terminaciones Nerviosas/metabolismo , Fibras Nerviosas/metabolismo , Sondas de Oligonucleótidos , ARN Complementario/biosíntesis , ARN Complementario/genética , Ratas , Ratas Wistar , Especificidad de la Especie , Fracciones Subcelulares

RESUMEN

A 17-month-old girl with type I classical citrullinaemia (CTLN1) presenting with early cirrhosis and unusual ultrastructural features of the liver is reported. The patient is homozygous for a splicing mutation in intron 15 of the argininosuccinate synthase gene.

Asunto(s)

Citrulinemia/complicaciones , Cirrosis Hepática/diagnóstico , Cirrosis Hepática/etiología , Argininosuccinato Sintasa/deficiencia , Argininosuccinato Sintasa/genética , Citrulinemia/genética , Citrulinemia/patología , Femenino , Homocigoto , Humanos , Lactante , Intrones/genética , Mutación , Empalme del ARN/genética

RESUMEN

Ciliary neurotrophic factor (CNTF) is primarily regarded as an astrocytic lesion factor, promoting neuronal survival and influencing plasticity processes in deafferented areas of the CNS. Postnatal loss of neurons in CNTF-deficient mice indicates a function of the factor also under physiological conditions. In the olfactory bulb, where neurogenesis, axo- and synaptogenesis continue throughout life, CNTF content is constitutively high. The cellular localization of CNTF in the rat olfactory bulb is not fully resolved, and species differences between mouse and rat are not yet characterized. In the present study, four different CNTF antibodies and double immunolabeling with specific markers for glial and neuronal cells were used to study the cellular localization of CNTF in rat and mouse olfactory bulb. Specificity of the detection was checked with tissue from CNTF-deficient mice, and investigations were complemented by immunolocalization of reporter protein in mice synthesizing beta-galactosidase under control of the CNTF promoter (CNTF lacZ-knock-in mice). In both species, CNTF localized to ensheathing cell nuclei, cell bodies and axon-enveloping processes. Additionally, individual axons of olfactory neurons were CNTF immunoreactive. Both CNTF protein content and immunoreaction intensity were lower in mice than in rats. Scattered lightly CNTF-reactive cells were found in the granular and external plexiform layers in rats. Some CNTF-positive cells were associated with immunoreactivity for the polysialylated form of the neural cell adhesion molecule, which is expressed by maturing interneurons derived from the rostral migratory stream. In CNTF lacZ-knock-in mice, beta-galactosidase reactivity was found in ensheathing cells of the olfactory nerve layer, and in cells of the glomerular, external plexiform and granular layers. The study proves that CNTF is localized in glial and neuronal structures in the rodent olfactory bulb. Results in mice provide a basis for investigations concerning the effects of a lack of the factor in CNTF-deficient mice.

Asunto(s)

Factor Neurotrófico Ciliar/análisis , Factor Neurotrófico Ciliar/deficiencia , Bulbo Olfatorio/química , Animales , Factor Neurotrófico Ciliar/genética , Femenino , Masculino , Ratones , Ratones Noqueados , Bulbo Olfatorio/ultraestructura , Ratas , Ratas Wistar

RESUMEN

Microanatomical compartments of the human spleen are yet under evaluation as most of the present information comes from experiments on animals with different anatomical structures. Immune staining of stromal and blood-born cells by cell surface antigens facilitates the differentiation of functional microanatomical compartmentalization of immune organs, including the spleen. Twenty-two specimens from healthy adult subjects with the average age of 35.6 +/- 13.8 (Range 17 to 58) years were included in this study. Monoclonal antibodies used in this study were supplied from the 5th, 6th and 7th International Workshops and Conferences on Human Leukocyte Differentiation Antigens. Tetraspan antigens presented a rather unique staining pattern in the human spleen, suggesting special roles for each (CD9, CD53, CD63, CD151 and CD231) in certain locations. Sinus lining cells presented a distinctive antigenic profile, sharing both endothelial cell (CD31, CD36, CD54, CD62P, CD102, CD105, CD106 and CD146) and macrophage lineage characteristics. The sheathed capillaries were not restricted to the perifollicular zone alone. Extracellular matrix receptors (CD49 a, CD49 b, CD49 c, CD49 e, CD49f, CD29 and CD44) stained the penicillary arterioles and vascular smooth muscle. These molecules were also found on the vascular endothelium. Leukocyte antigens (CD11a, CD11b, CD22, CD43, CD45, CD45RB, CD45RO and CD50) were mainly expressed in the white and red pulp of the spleen at different intensities, excluding the penicillary arterioles. Activation antigens (CD26, CD71 and CD98) presented a diffuse and broad staining pattern. In conclusion, microanatomical compartmentalization, microcirculation and function of the human spleen were evaluated using a wide panel of monoclonal antibodies.

Asunto(s)

Antígenos CD/análisis , Linfocitos/inmunología , Bazo/inmunología , Técnica del Anticuerpo Fluorescente Indirecta , Humanos , Inmunofenotipificación , Integrinas/análisis , Linfocitos/citología , Macrófagos/citología , Macrófagos/inmunología , Bazo/citología

RESUMEN

Rapid uptake of synaptically released glutamate via the high affinity glutamate transporter 1 (GLT1; EAAT2) is important for limiting transmitter signalling and prevents a harmful receptor overstimulation. So far, in the adult brain GLT1 protein has only been detected in astrocytes. Here, we describe the cDNA cloning of a variant of GLT1 from rat brain which is generated by alternative splicing at the 3'-end of the GLT1 cDNA. Reverse transcription-polymerase chain reaction revealed that the GLT1 variant message was present not only in brain, but also in peripheral organs. Northern blot analysis showed that in brain the mRNA of GLT1 (approximately 11 kb) is predominant while in the retina the mRNA of GLT1 variant (approximately 12.5 kb) prevails. In situ hybridization using cRNA and oligonucleotide probes, and immunocytochemistry using an antibody against a synthetic GLT1v peptide were applied in order to identify the cell types expressing GLT1 variant in the adult rat nervous system. GLT1 variant is preferentially expressed in neurons of the CNS and PNS, but is also detected in glial cells (oligodendrocytes, ependymal cells, epithelial cells of the plexus choroideus, satellite cells, and Schwann cells). In contrast to GLT1, GLT1 variant was only occasionally detected in astrocytes. Immunolabelling revealed a preferentially cytoplasmic (frequently granular) staining of neurons and glial cells, suggesting a localization of GLT1 variant protein in vesicle membranes. The studies provide evidence that the cellular expression of the GLT1 variant in the CNS is almost complementary to that of GLT1 and that the GLT1 variant does not seem to be restricted to the CNS.

Asunto(s)

Empalme Alternativo/genética , Transportador 2 de Aminoácidos Excitadores/genética , Transportador 2 de Aminoácidos Excitadores/metabolismo , Ácido Glutámico/metabolismo , Sistema Nervioso/metabolismo , Neuroglía/metabolismo , Neuronas/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Encéfalo/citología , Encéfalo/metabolismo , Plexo Coroideo/citología , Plexo Coroideo/metabolismo , Clonación Molecular , Ganglios Espinales/citología , Ganglios Espinales/metabolismo , Inmunohistoquímica , Masculino , Datos de Secuencia Molecular , Plexo Mientérico/citología , Plexo Mientérico/metabolismo , Sistema Nervioso/citología , Neuroglía/citología , Neuronas/citología , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Retina/citología , Retina/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células de Schwann/citología , Células de Schwann/metabolismo , Nervio Ciático/citología , Nervio Ciático/metabolismo , Médula Espinal/citología , Médula Espinal/metabolismo

RESUMEN

In brain, signaling pathways initiated by atrial natriuretic peptide, or transmitters which stimulate nitric oxide synthesis, increase cGMP as their second messenger. One important class of target molecules for cGMP is cGMP-dependent protein kinases, and in the present study, biochemical and immunocytochemical analyses demonstrate the widespread distribution of type II cGMP-dependent protein kinase in rat brain, from the cerebral cortex to the brainstem and cerebellum. Also, colocalization of cGMP-dependent protein kinase type II with its activator, cGMP, was found in several brain regions examined after in vitro stimulation of brain slices with sodium nitroprusside. In western blots, cGMP-dependent protein kinase type II was observed in all brain regions examined, although cerebellar cortex and pituitary contained comparatively less of the kinase. Immunocytochemistry revealed cGMP-dependent protein kinase type II in certain neurons, and occasionally in putative oligodendrocytes and astrocytes, however, its most striking and predominant localization was in neuropil. Electron microscopy examination of neuropil in the medial habenula showed localization of the kinase in both axon terminals and dendrites. As a membrane-associated protein, cGMP-dependent protein kinase type II often appeared to be transported to cell processes to a greater extent than being retained in the cell body. Thus, immunocytochemical labeling of cGMP-dependent protein kinase type II often did not coincide with the localization of kinase mRNA previously observed by others using in situ hybridization. We conclude that in contrast to cGMP-dependent protein kinase type I, which has a very restricted localization to cerebellar Purkinje cells and a few other sites, cGMP-dependent protein kinase type II is a very ubiquitous brain protein kinase and thus a more likely candidate for relaying myriad cGMP effects in brain requiring protein phosphorylation.

Asunto(s)

Encéfalo/enzimología , Isoenzimas/metabolismo , Animales , Western Blotting , Encéfalo/metabolismo , GMP Cíclico/metabolismo , Proteínas Quinasas Dependientes de GMP Cíclico/metabolismo , Inmunohistoquímica , Masculino , Óxido Nítrico/fisiología , Ratas , Ratas Endogámicas WKY , Sensibilidad y Especificidad , Coloración y Etiquetado , Distribución Tisular

RESUMEN

BACKGROUND: Inhabitants of the southeast of Turkey (ST) have been exposed since childhood to inhalation of asbestos, from a material containing tremolite, used for whitewashing. This has resulted in an increased incidence of malignant pleural mesothelioma (MPM). OBJECTIVES: To review the epidemiological features of MPM cases in ST; to calculate and compare the incidence with the previously reported ones. SUBJECTS AND METHODS: The study included 176 MPM cases from different places in ST. The incidence of MPM was calculated for those places according to the distribution of the cases. RESULTS: In the previously identified regions of asbestos (region 1) where the population had been informed of the danger with the soil some decades ago, the MPM incidence was decreased, as compared to the previous reports. The annual incidence of MPM in these places was found to be 42.9 per million in this study while it had been reported to be 105.5 per million in the previous studies. In contrast, the incidence that was reported previously to be 2.75 per million in the regions where asbestos exposure had not been identified before (region 2) was found to be 8.6 per million in this study. In region 2 the incidence of MPM increased even in the second half of the last decade (5.9 versus 11.9 per million). CONCLUSIONS: Use of asbestos-containing soil continues in different places in ST. Even if the use of this soil is abandoned today, MPM will be an important health problem in this region till the third or fourth decades of this century. Informing the villagers of the danger and preventing the use of this soil may result in a considerable decrease in the incidence of MPM.

Asunto(s)

Amianto/efectos adversos , Carcinógenos/efectos adversos , Exposición a Riesgos Ambientales/efectos adversos , Mesotelioma/epidemiología , Mesotelioma/etiología , Neoplasias Pleurales/epidemiología , Neoplasias Pleurales/etiología , Adulto , Distribución por Edad , Anciano , Amianto/análisis , Carcinógenos/análisis , Femenino , Humanos , Incidencia , Masculino , Mesotelioma/diagnóstico por imagen , Persona de Mediana Edad , Neoplasias Pleurales/diagnóstico por imagen , Radiografía , Factores de Tiempo , Turquía/epidemiología

RESUMEN

Trauma is a common cause of pulpal damage. In traumatic injuries, the first priority is to protect the vitality of pulps. But the time between the trauma and treatment must be short to preserve vital, noninflamed pulps. The aim of this study was to investigate the histopathological changes in pulpal tissues at different time periods after crown fractures. Twenty-three teeth with enamel and dentin fractures, with and without pulp exposure were evaluated. The reasons for seeking dental treatment were aesthetic consideration, pain, or discomfort. The extirpated pulps were histologically prepared for microscopical evaluation. There was myelin degeneration surrounding the axons and edema in the early posttraumatic stages (17 h). In the later stages (4 to 20 days), the tissues showed varying degrees of inflammation, and neuronal degeneration such as intramyelin edema, aberrant myelin synthesis, and axonal swelling.

Asunto(s)

Pulpa Dental/patología , Corona del Diente/lesiones , Fracturas de los Dientes/patología , Axones/patología , Colágeno/ultraestructura , Esmalte Dental/lesiones , Pulpa Dental/irrigación sanguínea , Pulpa Dental/inervación , Exposición de la Pulpa Dental/etiología , Exposición de la Pulpa Dental/patología , Dentina/lesiones , Edema/patología , Estética Dental , Humanos , Vaina de Mielina/patología , Neovascularización Fisiológica , Degeneración Nerviosa/patología , Neuronas/patología , Pulpitis/patología , Factores de Tiempo , Odontalgia/etiología

RESUMEN

In this study we examined the chorionic villi of 5 normal human placentas at 12-14 weeks of gestation ultrastructurally with regard to differentiation of the vascular components. The aim of the present report is to discuss the factors influencing vasculogenesis (in situ formation of blood vessels) at the ultrastructural level. Our observations have led us to think that the cytotrophoblast influences vasculogenesis in human chorionic villi. Mesenchymal-preendothelial cell groups were always found in very close association with the cytotrophoblast at the periphery of the villi, forming blood vessels. The cytotrophoblast probably attracts mesenchymal cells towards the margin of the villi by secreting vascular endothelial growth factor (VEGF). Once cells attach to the trophoblastic basement membrane they begin to differentiate into endothelial cells. This close structural relation between two cell types (cytotrophoblast and mesenchymal cells) may not be the only mechanism controlling vasculogenesis, but it seems to be one of the factors influencing the differentiation of mesenchymal cells into the endothelial cells of blood vessels in early human chorionic villi.

Asunto(s)

Vasos Sanguíneos/ultraestructura , Vellosidades Coriónicas/irrigación sanguínea , Vellosidades Coriónicas/ultraestructura , Neovascularización Fisiológica , Primer Trimestre del Embarazo , Vasos Sanguíneos/fisiología , Vellosidades Coriónicas/fisiología , Muestra de la Vellosidad Coriónica , Femenino , Humanos , Mesodermo/ultraestructura , Microscopía Electrónica , Embarazo , Trofoblastos/ultraestructura

RESUMEN

By enzyme-linked in situ hybridization (ISH), direct evidence is provided that acid-secreting intercalated cells (type A IC) of both the cortical and medullary collecting ducts of the rat kidney selectively express the mRNA of the kidney splice variant of anion exchanger 1 (kAE1) and no detectable levels of the erythrocyte AE1 (eAE1) mRNA. Using single-cell quantification by microphotometry of ISH enzyme reaction, medullary type A IC were found to contain twofold higher kAE1 mRNA levels compared with cortical type A IC. These differences correspond to the higher intensity of immunostaining in medullary versus cortical type A IC. Chronic changes of acid-base status induced by addition of NH(4)Cl (acidosis) or NaHCO3 (alkalosis) to the drinking water resulted in up to 35% changes of kAE1 mRNA levels in both cortical and medullary type A IC. These experiments provide direct evidence at the cellular level of kAE1 expression in type A IC and show moderate capacity of type A IC to respond to changes of acid-base status by modulation of kAE1 mRNA levels.

Asunto(s)

Acidosis/metabolismo , Alcalosis/metabolismo , Empalme Alternativo , Antiportadores/genética , Regulación de la Expresión Génica , Riñón/metabolismo , Cloruro de Amonio/farmacología , Animales , Antiportadores/análisis , Antiportadores de Cloruro-Bicarbonato , Cloruros/metabolismo , Eritrocitos/metabolismo , Regulación de la Expresión Génica/efectos de los fármacos , Variación Genética , Hibridación in Situ , Corteza Renal/metabolismo , Médula Renal/metabolismo , Túbulos Renales Colectores/metabolismo , Masculino , ARN Mensajero/genética , Ratas , Ratas Wistar , Bicarbonato de Sodio/farmacología , Transcripción Genética

RESUMEN

Dopamine, a major neurotransmitter in the vertebrate retina, is released from interplexiform cells and a restricted subset of amacrine cells. Dopamine effects vary between different retinal cell types, most likely due to differences in cell-specific receptor subtype expression. Identification of cells expressing receptors of the D2-subfamily (D2R, D3R, D4R) on a light microscopical level has rendered equivocal results, and no information is as yet available concerning the subcellular distribution of receptor protein. In the present study, D2R and D2/3R subtype-specific antisera, and D2R-, D3R- and D4R-specific oligonucleotide probes were used for ultrastructural and in situ hybridization analyses of the receptor subtype distribution in the rat retina. Light and electron microscopy showed that in addition to the known localization of intense D2R-immunoreactivity in all dopaminergic cells immunoreactive for tyrosine hydroxylase (TH), homogeneous, less intense D2R-immunoreactivity was also seen throughout the inner plexiform layer (IPL). Ultrastructurally, many additional amacrine cell processes devoid of TH-immunoreactivity at all levels of the inner plexiform layer were immunoreactive. D2R-immunoreactivity was found mainly on intracellular vesicles, and immunoreactivity associated with the plasma membrane was always extrasynaptic. No D2R-immunoreactivity was found in amacrine cell somata postsynaptic to the so-called dopaminergic 'ring endings'. Many D2R-mRNA reactive cells were observed throughout the inner nuclear layer. Morphologically, labelled cells resemble amacrines and bipolars but not horizontal cells. Reactivity with splice variant-specific oligonucleotide probes suggested that the D2LR variant is the predominant if not the only D2R isoform in the rat retina. D2R-mRNA reactivity was not observed in other retinal layers, in particular not in photoreceptor inner segments, which displayed D4R-mRNA reactivity. D3R-mRNA reactivity was not detected. The results indicate that D2-like responses are mediated through the D2R subtype, by an autoreceptor mechanism in dopaminergic cells, and by volume transmission in non-dopaminergic cells of the inner retina. D2-like responses in photoreceptors probably represent D4R activation.

Asunto(s)

Familia de Multigenes , Receptores de Dopamina D2/genética , Retina/química , Animales , Inmunohistoquímica , Hibridación in Situ , Microscopía Inmunoelectrónica , Sondas de Oligonucleótidos , ARN Mensajero/biosíntesis , Ratas , Ratas Wistar , Tirosina 3-Monooxigenasa/análisis