Expression of rat kidney anion exchanger 1 in type A intercalated cells in metabolic acidosis and alkalosis.
Am J Physiol
; 277(6): F841-9, 1999 12.
Article
en En
| MEDLINE
| ID: mdl-10600930
By enzyme-linked in situ hybridization (ISH), direct evidence is provided that acid-secreting intercalated cells (type A IC) of both the cortical and medullary collecting ducts of the rat kidney selectively express the mRNA of the kidney splice variant of anion exchanger 1 (kAE1) and no detectable levels of the erythrocyte AE1 (eAE1) mRNA. Using single-cell quantification by microphotometry of ISH enzyme reaction, medullary type A IC were found to contain twofold higher kAE1 mRNA levels compared with cortical type A IC. These differences correspond to the higher intensity of immunostaining in medullary versus cortical type A IC. Chronic changes of acid-base status induced by addition of NH(4)Cl (acidosis) or NaHCO3 (alkalosis) to the drinking water resulted in up to 35% changes of kAE1 mRNA levels in both cortical and medullary type A IC. These experiments provide direct evidence at the cellular level of kAE1 expression in type A IC and show moderate capacity of type A IC to respond to changes of acid-base status by modulation of kAE1 mRNA levels.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acidosis
/
Regulación de la Expresión Génica
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Empalme Alternativo
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Antiportadores
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Alcalosis
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Riñón
Límite:
Animals
Idioma:
En
Revista:
Am J Physiol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos