RESUMEN

In the present work, tomato peels were pre-treated using combination of ultrasound and enzyme co-immobilized amino-functionalized magnetic nanoparticles (AMNPs) for the efficient release of lycopene. To achieve maximum activity of enzymes in the co-immobilized form, optimization of several parameters were carried out. Moreover, the influence of ultrasound and enzyme co-immobilized magnetic nanoparticles on lycopene release was studied. Maximum lycopene release was obtained at 3% (w/w) enzyme co-immobilized AMNPs, pH 5.0, temperature of 50 °C, at 10 W ultrasound power and 20 min incubation time. After enzymatic pre-treatment, lycopene from the pre-treated mixture was extracted and separated using tri-solvent extraction method. Maximum recovery of lycopene using solvent extraction was obtained at 50 °C, 90 min of incubation time and agitation speed of 150 rpm. The presence of lycopene in the extract was confirmed by FT-IR, UV-vis spectroscopy and HPLC analysis. The co-immobilized bio-catalyst showed excellent reusability giving more than 50% lycopene yield even after 6th cycles of reuse.

Asunto(s)

Carotenoides/aislamiento & purificación , Celulasa/metabolismo , Enzimas Inmovilizadas/metabolismo , Poligalacturonasa/metabolismo , Solanum lycopersicum/química , Sonicación/métodos , Biocatálisis , Cromatografía Líquida de Alta Presión , Almacenamiento de Alimentos , Calor , Concentración de Iones de Hidrógeno , Licopeno , Magnetismo , Nanopartículas , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de Fourier , Factores de Tiempo