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BACKGROUND: Leptospirosis is the world's most common zoonotic disease. Mitigation and control rely on pathogen identification and understanding the roles of potential reservoirs in cycling and transmission. Underreporting and misdiagnosis obscure the magnitude of the problem and confound efforts to understand key epidemiological components. Difficulties in culturing hamper the use of serological diagnostics and delay the development of DNA detection methods. As a result, especially in complex ecosystems, we know very little about the importance of different mammalian host species in cycling and transmission to humans. METHODOLOGY/PRINCIPAL FINDINGS: We sampled dogs from five indigenous Kichwa communities living in the Yasuní National Park in the Ecuadorian Amazon basin. Blood and urine samples from domestic dogs were collected to assess the exposure of these animals to Leptospira and to identify the circulating species. Microscopic Agglutination Tests with a panel of 22 different serovars showed anti-leptospira antibodies in 36 sampled dogs (75%), and 7 serogroups were detected. Two DNA-based detection assays revealed pathogenic Leptospira DNA in 18 of 19 dog urine samples (94.7%). Amplicon sequencing and phylogenetic analysis of 16S rRNA and SecY genes from 15 urine samples revealed genetic diversity within two of three different Leptospira species: noguchii (n = 7), santarosai (n = 7), and interrogans (n = 1). CONCLUSIONS/SIGNIFICANCE: The high prevalence of antibodies and Leptospira DNA provides strong evidence for high rates of past and current infections. Such high prevalence has not been previously reported for dogs. These dogs live in the peridomestic environment in close contact with humans, yet they are free-ranging animals that interact with wildlife. This complex web of interactions may explain the diverse types of pathogenic Leptospira observed in this study. Our results suggest that domestic dogs are likely to play an important role in the cycling and transmission of Leptospira. Future studies in areas with complex ecoepidemiology will enable better parsing of the significance of genotypic, environmental, and host characteristics.
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Leptospira , Leptospirosis , Animales , Perros , Humanos , Ecosistema , Filogenia , ARN Ribosómico 16S/genética , Leptospirosis/epidemiología , Leptospirosis/veterinaria , Animales Salvajes , ADN , MamíferosRESUMEN
Background: Leptospirosis is the world's most common zoonotic disease. Mitigation and control rely on pathogen identification and understanding the roles of potential reservoirs in cycling and transmission. Underreporting and misdiagnosis obscure the magnitude of the problem and confound efforts to understand key epidemiological components. Difficulties in culturing hamper the use of serological diagnostics and delay the development of DNA detection methods. As a result, especially in complex ecosystems, we know very little about the importance of different mammalian host species in cycling and transmission to humans. Methodology/Principal Findings: We sampled five indigenous Kichwa communities living in the Yasuní National Park in the Ecuadorian Amazon basin. Blood and urine samples from domestic dogs were collected to assess the exposure of these animals to Leptospira, and to identify the circulating species. Microscopic Agglutination Tests with a panel of 22 different serovars showed anti-leptospira antibodies in 36 sampled dogs (75%), and 10 serotypes were detected. Two DNA-based detection assays revealed pathogenic Leptospira DNA in 18 of 19 dog urine samples (94.7%). Amplicon sequencing and phylogenetic analysis of 16s rDNA and SecY genes from 15 urine samples revealed genetic diversity within two of three different Leptospira species: noguchii (n=7), santarosai (n=7), and interrogans (n=1). Conclusions/Significance: The high prevalence of antibodies and Leptospira DNA provides strong evidence for high rates of past and current infections. Such high prevalence has not been previously reported for dogs. These dogs live in the peridomestic environment in close contact with humans, yet they are free-ranging animals that interact with wildlife. This complex web of interactions may explain the diverse types of pathogenic Leptospira observed in this study. Our results suggest that domestic dogs are likely to play an important role in the cycling and transmission of Leptospira. Future studies in areas with complex ecoepidemiology will enable better parsing of the significance of genotypic, environmental, and host characteristics.
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BACKGROUND: This research aimed to determine the porosity and particle size distribution in canned Vienna-type sausages using digital image analysis (DIA) on photographs captured with a digital camera and applying a Monte Carlo simulation. The methodology determined morphometric parameters (area and Feret diameter) by DIA of transverse and longitudinal sections of canned sausages. Those images were previously contrast enhanced, color threshold adjusted, and binarized. Subsequently, the estimation of the pore volume was carried out from the inverse Gaussian distributions of Feret diameter and area, as well as the porosity, using Monte Carlo simulation. RESULTS: The pores had an average Feret diameter of 0.335 mm and an average area of 0.085 mm2 . The highest estimated bivariate kernel density was presented for the smallest pores (around 0.02 mm2 in area and 0.25 mm in diameter). Simulation average values of pore volume, assumed as a cylinder, and porosity were 1.455 mm3 and 0.737 respectively. The average porosity value was consistent with the value experimentally estimated by the indirect method, in concordance with the definition of porosity, which was 0.715, presenting a mean relative percentage error of 3.08% concerning the estimated experimental value as well. CONCLUSION: This research presents interesting perspectives for the quantitative analysis of the microstructure of food and biological materials through a novel, low-cost, reliable, and fast proposal. Moreover, this is the first study to report the porosity determination in canned sausages by DIA. © 2022 Society of Chemical Industry.
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Porosidad , Método de Montecarlo , Simulación por ComputadorRESUMEN
BACKGROUND: A good treatment for type 1 diabetes mellitus (T1DM) requires accurate measurements of blood glucose levels. Continuous glucose monitors (CGM) measure the glucose concentration in the interstitial fluid of the abdominal subcutaneous adipose tissue. However, glucose measured in the abdominal interstitial fluid does not represent blood glucose concentrations accurately due to the complex blood transport through the body and glucose diffusion in interstitial fluid. METHODS: To gain insight into this problem, a phenomenological-based semiphysical model (PBSM) of glucose transport by volumetric flow and diffusion from the bloodstream to interstitial fluid was constructed. A published 10-step modeling procedure was used to obtain a model for glucose transport time through the blood vessels and from the blood capillaries to the interstitial fluid, glucose diffusion within the interstitial fluid, and glucose diffusion through the semipermeable coating of the sensor needle. For this model, a healthy person is considered at rest with average parameters. RESULTS: The simulations performed using the PBSM allow obtaining the glucose transport time from the liver to the sensor needle. In this way, it is possible to reconstruct an accurate dynamic measurement of blood glucose from the measurements in the interstitial fluid of the abdominal subcutaneous adipose tissue. CONCLUSIONS: PBSMs with parameters interpretability illustrate the connection of glucose concentrations in the interstitial fluid with that currently in the blood. Implementing this model in a CGM will result in more reliable measurements of blood glucose levels for T1DM treatment.
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Diabetes Mellitus Tipo 1 , Grasa Subcutánea Abdominal , Tejido Adiposo , Glucemia , Glucosa , Humanos , HígadoRESUMEN
This study aims to build a model for predicting the hardness of meat products by considering their protein fractions and protein structural changes during production. Protein solubility is considered an indicator of protein structural changes. The obtained model results show that structural changes of sarcoplasmic and myofibrillar proteins occur during production. The gelling capacity is formed by the effect of the three protein fractions, namely myofibrillar, sarcoplasmic and stromal. The obtained model allows the prediction of the hardness of meat products based on their protein fraction contents with error below 5%, thus reaching a significant adjustment between real process data and the simulated model.
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BACKGROUND: The efficacy of non-narcotic analgesics is mostly supported by randomized, placebo-controlled trials with no comparison with ordinary practice. Additionally, systematic reviews of these placebo-controlled trials have failed to determine clinically meaningful dose-response effect. METHODS: In this double-blind, randomized trial, patients undergoing elective inguinal, umbilical or epigastric herniorrhaphy under general anesthesia were assigned to receive 15 mg/kg (D15 group) vs. 40 mg/kg (D40 group) of dipyrone intravenously during surgery. The primary outcome was the incidence of moderate to severe pain with movement during the recovery room phase. The secondary outcomes were morphine consumption, incidence of vomiting, and Ramsay score (sedation scale). RESULTS: One hundred sixty-two patients were enrolled and analyzed for the primary and secondary outcomes. Relative to the D15 group, the D40 group showed a lower incidence of moderate to severe pain in the first 30 minutes (61% and 40%; P value < 0.05); lower cumulative morphine consumption during the recovery period (3.85 vs. 2.55 mg, P value < 0.006) as well as a lower incidence of vomiting (15.8% vs. 2.5%, P value < 0.005). In addition, more cases of sedation were recorded in the D15 group than in the D40 group (17 vs. 10 cases). There were no serious adverse effects attributed to dipyrone in either group. CONCLUSION: This trial shows a dose-response effect of 40 mg/kg over 15 mg/kg of intravenous dipyrone based on better movement-induced pain control, lower morphine consumption and fewer opioid-related side effects.
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Analgésicos no Narcóticos/uso terapéutico , Dipirona/uso terapéutico , Herniorrafia , Dolor Postoperatorio/tratamiento farmacológico , Adolescente , Adulto , Anciano , Analgésicos no Narcóticos/efectos adversos , Dipirona/efectos adversos , Método Doble Ciego , Femenino , Humanos , Masculino , Persona de Mediana Edad , Morfina/efectos adversos , Morfina/uso terapéutico , Adulto JovenRESUMEN
Feeding habits and trophic ecology of the fish Lutjanus griseus (Pisces: Lutjanidae) associated with submerged vegetation in Laguna de Términos, Campeche, Mexico. In Campeche, Mexico, the Laguna de Términos has Thalassia testudinum beds inhabited by the grey snapper, Lutjanus griseus (Linnaeus 1758). Along one year, we collected 994 individuals and 672 had food in their stomachs; we recorded number, weight, and frequency of trophic groups, and the index of relative importance. Dominant food components were Farfantepenaeus duorarum and Palemonetes octaviae, Eucinostomus gula (Cuvier 1830) and Libinia dubia. Salinity and temperature were related to the number of individuals collected and with greater fish consumption by large L. griseus. The trophic niche breadth index was calculated for six size-classes of fish. The smallest and the largest fish had the lowest index values, whereas medium-size snappers had higher values. This snapper has a preference for habitats with submerged vegetation. The percentage of fish with food in their stomachs was higher during dark hours, providing evidence of the nocturnal habits of this fish. Rev. Biol. Trop. 55 (3-4): 989-1004. Epub 2007 December, 28.
Se presentan los resultados de un estudio de los hábitos alimenticios de Lutjanus griseus asociado a la vegetación sumergida en El Cayo, Laguna de Términos, Campeche, México. La investigación se realizó siguiendo un diseño de muestreo intensivo tanto en escala espacial como temporal. Se recolectaron un total de 994 individuos, de los cuales 672 tenía alimento en sus estómagos. Se analizaron los porcentajes en número, peso y frecuencia de los grupos tróficos, así como el índice de importancia relativa. Los componentes alimenticios dominantes fueron Farfantepenaeus duorarum, Palaemonetes octavie, Eucinostomus gula y Libinia dubia. La salinidad y la temperatura se relacionaron con el número de individuos colectados así como con un incremento en el consumo de peces por los individuos de mayor talla. Se calculó el índice de diversidad de dieta para seis clases de talla de los peces, examinándose su variación a través del año. Los peces de menor y mayor talla mostraron los valores más bajos de este índice, mientras que los organismos de las tallas intermedias presentaron los valores más altos. Los resultados mostraron que L. griseus tiene preferencia por hábitats con vegetación sumergida. El porcentaje de peces con alimento en sus estómagos fue mayor durante la noche que durante el día, evidenciando los hábitos nocturnos de esta especie.
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Animales , Ecosistema , Peces/fisiología , Conducta Alimentaria/fisiología , Agua Dulce , Peces/clasificación , Contenido Digestivo , México , Estaciones del AñoRESUMEN
An extended dynamical model for growth and sporulation of Bacillus thuringiensis subsp. kurstaki in an intermittent fed-batch culture with total cell retention is proposed. This model differs from reported models, by including dynamics for natural death of cells and substrate consumption for cell maintenance. The proposed model uses sigmoid functions to describe these kinetic parameters. Equations for time evolution of substrate, vegetative, sporulated and total cell concentration were taken from previous works. Model parameters were determined from batch experimental data obtained in pilot plant. Parameter identification was developed in two stages: (1) coarse identification using a multivariable optimization with constraints algorithm, (2) fine identification by heuristic fit of model parameters looking for a minimal model error. The proposed model estimates adequate time evolution of the process variables with a mean error of 2.6% on substrate concentration and 6.7% on biomass concentration.
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Bacillus thuringiensis/crecimiento & desarrollo , Bacillus thuringiensis/fisiología , Modelos Biológicos , Reactores Biológicos , Fermentación , Cinética , Proyectos Piloto , Esporas Bacterianas/fisiología , Biología de SistemasRESUMEN
La calidad de vida relativa a salud se está constituyendo en un instrumento fiable para evaluar la calidad de vida relativa a la función visual en pacientes con afecciones oculares. En este estudio esta se evalúa mediante el cuestionario de calidad de vida relativa a la visión (VQOL-15) en pacientes con diagnóstico de catarata senil, previo a la cirugía y 4 meses después de ella. Se realizó un estudio de intervención prospectivo comparativo al total de pacientes operados de cirugía de cataratas en el período comprendido entre 1 de julio de 2005 y 1 de julio de 2006. Se encontró que la edad media de los pacientes estudiados fue de 73 años, el color de la piel predominante fue la blanca y el sexo, el masculino. Se demostró que la calidad de vida previa a la cirugía marcó una media de 44, 1 y después de esta 80,6 con un valor p = 0,0000. Se concluye que el VQOL-15 es un instrumento de gran utilidad y que se ajusta a nuestro medio, para medir calidad de vida relativa a visión en pacientes con cataratas(AU)
In recent times, the health-related quality of life is a reliable tool to evaluate vision-related quality of life in patients with ocular affections. In the present study, the vision-related quality of life questionnaire (VQOL-15) was used to evaluate this important aspect in patients with senile cataract before surgery and 4 months after. A comparative prospective intervention study was performed on patients operated from cataract, before and after surgery, in the period of July 1st, 2005 to July 1st, 2006. It was found that the average age was 73 years and they were mostly Caucasians and males. It was demonstrated that the quality of life before surgery was 44,1 and afterwards, it was 80,6, with p=0.0000. The conclusion was that VQOL-15 is a very useful tool that may be adjusted to our context in order to measure vision-related quality of life in patients with cataract(AU)
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Humanos , Estudios Prospectivos , Calidad de Vida , Extracción de CatarataRESUMEN
La calidad de vida relativa a salud se está constituyendo en un instrumento fiable para evaluar la calidad de vida relativa a la función visual en pacientes con afecciones oculares. En este estudio esta se evalúa mediante el cuestionario de calidad de vida relativa a la visión (VQOL-15) en pacientes con diagnóstico de catarata senil, previo a la cirugía y 4 meses después de ella. Se realizó un estudio de intervención prospectivo comparativo al total de pacientes operados de cirugía de cataratas en el período comprendido entre 1 de julio de 2005 y 1 de julio de 2006. Se encontró que la edad media de los pacientes estudiados fue de 73 años, el color de la piel predominante fue la blanca y el sexo, el masculino. Se demostró que la calidad de vida previa a la cirugía marcó una media de 44, 1 y después de esta 80,6 con un valor p = 0,0000. Se concluye que el VQOL-15 es un instrumento de gran utilidad y que se ajusta a nuestro medio, para medir calidad de vida relativa a visión en pacientes con cataratas.
In recent times, the health-related quality of life is a reliable tool to evaluate vision-related quality of life in patients with ocular affections. In the present study, the vision-related quality of life questionnaire (VQOL-15) was used to evaluate this important aspect in patients with senile cataract before surgery and 4 months after. A comparative prospective intervention study was performed on patients operated from cataract, before and after surgery, in the period of July 1st, 2005 to July 1st, 2006. It was found that the average age was 73 years and they were mostly Caucasians and males. It was demonstrated that the quality of life before surgery was 44,1 and afterwards, it was 80,6, with p=0.0000. The conclusion was that VQOL-15 is a very useful tool that may be adjusted to our context in order to measure vision-related quality of life in patients with cataract.
Asunto(s)
Humanos , Extracción de Catarata , Estudios Prospectivos , Calidad de VidaRESUMEN
In Campeche, Mexico, the Laguna de Términos has Thalassia testudinum beds inhabited by the grey snapper, Lutjanus griseus (Linnaeus 1758). Along one year, we collected 994 individuals and 672 had food in their stomachs; we recorded number, weight, and frequency oftrophic groups, and the index of relative importance. Dominant food components were Farfantepenaeus duorarum and Palemonetes octaviae, Eucinostomus gula (Cuvier 1830) and Libinia dubia. Salinity and temperature were related to the number of individuals collected and with greater fish consumption by large L. griseus. The trophic niche breadth index was calculated for six size-classes of fish. The smallest and the largest fish had the lowest index values, whereas medium-size snappers had higher values. This snapper has a preference for habitats with submerged vegetation. The percentage of fish with food in their stomachs was higher during dark hours, providing evidence of the nocturnal habits of this fish.
Asunto(s)
Ecosistema , Conducta Alimentaria/fisiología , Peces/fisiología , Animales , Peces/clasificación , Agua Dulce , Contenido Digestivo , México , Estaciones del AñoRESUMEN
En un estudio sobre 70 ojos operados de glaucoma, a los que se les realizó una trabeculectomía convencional con uso de 5-fluorouracilo transoperatorio, se excluyeron los ojos con alto riesgo de cierre posoperatorio de la fistula y se les realizaron varios controles posoperatorios hasta el 1½ año. Se observaron complicaciones propias de la cirugía filtrante sin antimetabolitos, que mejoraron con el tratamiento habitual, y se logró un control adecuado de la presión intraocular sin necesidad de reoperaciones o de medicación hipotensora, lo que hace pensar en resultados prometedores con este tipo de cirugía(AU)
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Humanos , Masculino , Femenino , Trabeculectomía/métodos , Glaucoma/cirugía , Fluorouracilo/uso terapéutico , Cuidados Intraoperatorios , Cirugía Filtrante , Factores de Riesgo , Procedimientos Quirúrgicos Oftalmológicos/métodosRESUMEN
En un estudio sobre 70 ojos operados de glaucoma, a los que se les realizó una trabeculectomía convencional con uso de 5-fluorouracilo transoperatorio, se excluyeron los ojos con alto riesgo de cierre posoperatorio de la fistula y se les realizaron varios controles posoperatorios hasta el 1½ año. Se observaron complicaciones propias de la cirugía filtrante sin antimetabolitos, que mejoraron con el tratamiento habitual, y se logró un control adecuado de la presión intraocular sin necesidad de reoperaciones o de medicación hipotensora, lo que hace pensar en resultados prometedores con este tipo de cirugía
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Humanos , Masculino , Femenino , Cirugía Filtrante , Fluorouracilo , Glaucoma , Cuidados Intraoperatorios , Procedimientos Quirúrgicos Oftalmológicos/métodos , Factores de Riesgo , TrabeculectomíaRESUMEN
BACKGROUND: There is a 7% up to 32% prevalence of psychiatric problems in the pediatric population. AIM: To study the epidemiological features of children and adolescents attended in a psychiatric outpatient clinic. MATERIAL AND METHODS: All admissions to a psychiatric outpatient clinic, of people aged 18 years old or less, during 1998, were reviewed. The causes of consultation and the diagnoses were classified according to diagnostic manuals (ICPC-WONCA and DSM-IV respectively), by three experienced psychiatrists. RESULTS: The files of 376 patients were reviewed (200 males, mean age of females 12 years old, mean age of males 10.5 years old). Seventy three percent consulted spontaneously. The main causes of consultation were anxiety disorders and adolescence problems (28%). The main diagnoses were attention deficit disorder and disruptive behavior, adaptative problems, mood and anxiety disorders. There were gender differences in the diagnoses. Women attended a mean of 6.3 sessions and men attended a mean of 4 sessions. Fifty nine percent abandoned treatment. CONCLUSIONS: There is a limited access to mental health care. The low compliance with treatments must be studied and improved.
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Trastornos Mentales/epidemiología , Adolescente , Instituciones de Atención Ambulatoria , Niño , Preescolar , Chile/epidemiología , Femenino , Humanos , Lactante , Masculino , Trastornos Mentales/diagnóstico , Prevalencia , Sector Privado , Estudios RetrospectivosRESUMEN
Realizamos un estudio comparativo de 2 técnicas quirúrgicas para la cirugía del Pterigión Primario. Nuestra muestra estuvo conformada por 100 pacientes portadores del Pterigión Primario Grado II, que fueron enviados a una consulta creada al efecto, según los criterios de inclusión y exclusión establecidos. Les fue llenada una encuesta. Según el método de muestreo aleatorio por conglomerados en el tiempo del Programa ALE de la Facultad de Ciencias Médicas de Cienfuegos. A 50 de estos pacientes se les realizó la autoplastia conjuntival y al resto la exéresis simple; estos pacientes se siguieron en consulta evolutivamente durante 6 meses. Comparamos los resultados obtenidos con la utilización de las 2 técnicas y encontramos que la autoplastia conjuntival resultó ser más eficaz; pues la frecuencia de complicaciones fue solamente del 6,0 porciento; mientras que con la técnica convencional la recidiva es del 20,0 porciento; por lo que esperamos resultados alentadores en cuanto al tratamiento del Pterigión Primario(AU)
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Humanos , Masculino , Femenino , Pterigion/cirugía , Procedimientos Quirúrgicos Oftalmológicos/métodosRESUMEN
Realizamos un estudio comparativo de 2 técnicas quirúrgicas para la cirugía del Pterigión Primario. Nuestra muestra estuvo conformada por 100 pacientes portadores del Pterigión Primario Grado II, que fueron enviados a una consulta creada al efecto, según los criterios de inclusión y exclusión establecidos. Les fue llenada una encuesta. Según el método de muestreo aleatorio por conglomerados en el tiempo del Programa ALE de la Facultad de Ciencias Médicas de Cienfuegos. A 50 de estos pacientes se les realizó la autoplastia conjuntival y al resto la exéresis simple; estos pacientes se siguieron en consulta evolutivamente durante 6 meses. Comparamos los resultados obtenidos con la utilización de las 2 técnicas y encontramos que la autoplastia conjuntival resultó ser más eficaz; pues la frecuencia de complicaciones fue solamente del 6,0 porciento; mientras que con la técnica convencional la recidiva es del 20,0 porciento; por lo que esperamos resultados alentadores en cuanto al tratamiento del Pterigión Primario
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Humanos , Masculino , Femenino , Procedimientos Quirúrgicos Oftalmológicos/métodos , PterigionRESUMEN
Este es el primer y único y primer trabajo en Chile sobre la salud mental en niños y adolescente usuarios del sistema institucional privado. El objetivo de este trabajo es conocer algunas características epidemiológicas de la población consultante. Se revisaron y analizaron en forma retrospectiva todos los ingresos realizados durante el año 1998. El protocolo de recolección de datos fue procesado mediante un programa mediante un programa Epi Info. Los motivos de consulta y diagnósticos fueron clasificados según el ICPC-WONCA y el DSM-IV respectivamente, todo esto realizado por tres psiquiatras capacitados. La muestra consistió en 376 personas entre 1 y 18 años de edad, siendo el promedio de 12 años para las niñas y 10,5 para los niños. El mayor porcentaje de consultas se concentra en el período otoño-invierno y la principal fuente de derivación fue la consulta espontánea, seguida por la escolar. Los motivos de consultas son diversos destacando las conductas ansiosas y problemas de los adolescentes. Entre los diagnósticos destacan los Transtornos por Déficit Atencional, los Transtornos Adaptativos y luego vienen los Transtornos de Ansiedad y los Transtornos Afectivos por su frecuencia. Se realizan comparaciones con la escasa información disponibles en esta área, a pesar de no ser metodológicamente comparables. Los resultados no concuerdan en su totalidad con lo encontrado. Es necesaria mayor investigación en el área
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Humanos , Masculino , Adolescente , Femenino , Recién Nacido , Lactante , Preescolar , Salud Mental , Sector Privado , Derivación y Consulta/estadística & datos numéricos , Trastornos de Ansiedad , Trastorno por Déficit de Atención con Hiperactividad , Chile , Composición Familiar , Servicios de Salud Mental , Trastornos del Humor , Síntomas Psíquicos , Estudios Retrospectivos , Trastornos de Adaptación/epidemiologíaRESUMEN
Se realizó la evaluación del impacto de la estrategia de canalización ampliada en la inducción de la demanda de actividades de promoción y prevención de los programas Control Prenatal (CP), carcinoma de cerviz, carcinoma de mama, y planificación, durante el periodo comprendido entre febrero y noviembre de 1999...
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Femenino , Embarazo , Promoción de la Salud , Salud de la Mujer , Servicios de Salud Reproductiva , Mujeres , Necesidades y Demandas de Servicios de SaludRESUMEN
Se realizó un estudio descriptivo a un grupo de niños que asistieron a consulta de oftalmología del Hospital Pediátrico Provincial de Cienfuegos, durante un período de dos meses con Xeroftalmía demostrada por la prueba de Citología de Impresión Conjuntival, cuyas edades estaban comprendidas entre 5 y 14 años. Se les realizó un examen oftalmológico detallado de anexos y córnea, incluyendo la tinción con fluoresceína y la prueba del tiempo de desintegración de la película de lágrimas y se relacionó ese grupo con otras posibles afecciones sistémicas causadas por el déficit de vitamina A. Sólo se diagnosticó xerosis conjuntival y corneal ligera asociada en la mayoría de los casos a enfermedad respiratoria aguda y parasitismo intestinal.
A descriptive study of a group of children aged 5-14 that attended the Ophthalmology Department of the Provincial Pediatric Hospital of Cienfuegos during months with xerophthalmia demostrated by the test of Conjunctival Impression Cytology was carried out. A detailled ophthalmological examination of adnexa and cornea was made, including the staining with fluorescein and the test of the of desintegration of the lacrimal pellicle. This group was related to other possible systemic caused by vitamin A deficiency. Only mild conjunctival and croneal xerosis was diagnosed. It was mostly associated with acute respiratory disease and intestinal parasitism.