RESUMEN
Thirty nine milk handlers from a factory of dairy products in the Province of Buenos Aires were examined for their nasal carriage of S. aureus strains capable of producing toxic-shock-syndrome toxin-1 (TSST-1). In addition, chance samples of handled foods, crude milk and milky fermented derivates (MFD) were studied. Strain isolation was made on Mannitol Salt Agar and on Baird-Parker Agar. Typical colonies were identified by their biochemical properties. Cultures that were found to be S. aureus were selected for analysis of the TSST-1 production. Eight milk handlers (20.5%) were carriers of S. aureus strains. Seven isolates (87.5%) were classified as biotype A (human ecovar) and 1(12.5%) was classified as biotype B (swine and poultry ecovar). Three out of 8 S. aureus biotype A isolates (37.5%), produced TSST-1. Taking into account the number of milk food handlers sampled (39), the carried rate of toxigenic strains was 7.6%. Three S. aureus strains were isolated from crude milk; 1(33.3%) was classified as biotype B and 2(66.6%) as biotype C (cattle and sheep ecovar). Thirteen S. aureus strains were isolated from MDF; 5(38.0%) were classified as biotype A, 1(7.7%) as belonging to biotype B and 7(53.8%) as belonging to biotype C. None of them had the ability to produce TSST-1.
Asunto(s)
Toxinas Bacterianas , Portador Sano/epidemiología , Productos Lácteos , Enterotoxinas/aislamiento & purificación , Contaminación de Alimentos , Manipulación de Alimentos , Microbiología de Alimentos , Infecciones Estafilocócicas/transmisión , Staphylococcus aureus/aislamiento & purificación , Superantígenos , Animales , Argentina/epidemiología , Humanos , Leche , Cavidad Nasal/microbiología , Staphylococcus aureus/metabolismoRESUMEN
Thirty nine milk handlers from a factory of dairy products in the Province of Buenos Aires were examined for their nasal carriage of S. aureus strains capable of producing toxic-shock-syndrome toxin-1 (TSST-1). In addition, chance samples of handled foods, crude milk and milky fermented derivates (MFD) were studied. Strain isolation was made on Mannitol Salt Agar and on Baird-Parker Agar. Typical colonies were identified by their biochemical properties. Cultures that were found to be S. aureus were selected for analysis of the TSST-1 production. Eight milk handlers (20.5
) were carriers of S. aureus strains. Seven isolates (87.5
) were classified as biotype A (human ecovar) and 1(12.5
) was classified as biotype B (swine and poultry ecovar). Three out of 8 S. aureus biotype A isolates (37.5
), produced TSST-1. Taking into account the number of milk food handlers sampled (39), the carried rate of toxigenic strains was 7.6
. Three S. aureus strains were isolated from crude milk; 1(33.3
) was classified as biotype B and 2(66.6
) as biotype C (cattle and sheep ecovar). Thirteen S. aureus strains were isolated from MDF; 5(38.0
) were classified as biotype A, 1(7.7
) as belonging to biotype B and 7(53.8
) as belonging to biotype C. None of them had the ability to produce TSST-1.
RESUMEN
Staphylococcus sp was investigated in the female lower genital tract of 102 healthy women aged between 18 and 48 years in San Luis, Argentina. Three hundred and six samples were obtained from labia, introitus and vagina (posterior fornix). Samples were plated on sheep blood, mannitol salt and Baird-Parker media. Strains were identified by tube coagulase test; thermonuclease, fibrinolysin, pigment and hemolysin production; glucose and mannitol utilization and novobiocin sensitivity. Antibiotic susceptibility was assayed. Strains were examined for their ability to produce staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 (TSST-1). Fourteen women (13.7%) had S. aureus in one or more samples: 10.7% labia, 3.9% introitus and 3.9% vaginal. All strains were sensitive to cephalotin, clindamycin, erythromycin, gentamycin and chloramphenicol; 21.0% were intermediate to methicillin; 15.7% were resistant to methicillin, 94.7% to penicillin and 21.0% to tetracycline. Three strains (15.7%) produced SEB, three (15.7%) SED, one (5.7%) SEC and three (15.7%) TSST-1. Only one strain (5.7%) produced both SEB and TSST-1. All strains produced hemolysins. Coagulase negative staphylococci were found in 40.1% of vaginal samples: S. epidermidis (32.2%) and S. saprophyticus (9.8%) were identified.
Asunto(s)
Toxinas Bacterianas , Enterotoxinas/análisis , Staphylococcus aureus/aislamiento & purificación , Superantígenos , Vagina/microbiología , Vulva/microbiología , Adolescente , Adulto , Femenino , Humanos , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Staphylococcus aureus/metabolismoRESUMEN
Staphylococcus sp was investigated in the female lower genital tract of 102 healthy women aged between 18 and 48 years in San Luis, Argentina. Three hundred and six samples were obtained from labia, introitus and vagina (posterior fornix). Samples were plated on sheep blood, mannitol salt and Baird-Parker media. Strains were identified by tube coagulase test; thermonuclease, fibrinolysin, pigment and hemolysin production; glucose and mannitol utilization and novobiocin sensitivity. Antibiotic susceptibility was assayed. Strains were examined for their ability to produce staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 (TSST-1). Fourteen women (13.7
) had S. aureus in one or more samples: 10.7
labia, 3.9
introitus and 3.9
vaginal. All strains were sensitive to cephalotin, clindamycin, erythromycin, gentamycin and chloramphenicol; 21.0
were intermediate to methicillin; 15.7
were resistant to methicillin, 94.7
to penicillin and 21.0
to tetracycline. Three strains (15.7
) produced SEB, three (15.7
) SED, one (5.7
) SEC and three (15.7
) TSST-1. Only one strain (5.7
) produced both SEB and TSST-1. All strains produced hemolysins. Coagulase negative staphylococci were found in 40.1
of vaginal samples: S. epidermidis (32.2
) and S. saprophyticus (9.8
) were identified.