RESUMEN

CDC25Mm, a mouse Ras-Guanine nucleotide Exchange Factor, is specifically expressed as a product of 140 kDa (p140) in the postnatal and adult brain. Immunohistochemical analysis indicates that it is present throughout the brain particularly concentrated in discrete punctate structures. Subcellular fractionation of the mouse brain shows that p140 is present in synaptosomes but not in highly purified synaptic vesicles. Moreover, isolated postsynaptic densities (PSDs) are largely enriched in CDC25Mm. This protein can be phosphorylated by calcium/calmodulin kinase II, the most abundant protein in PSDs. Altogether these results suggest that CDC25Mm is present at synaptic junctions and that it may be involved in synaptic signal transduction leading to Ras activation.

Asunto(s)

Encéfalo/citología , Proteínas de Ciclo Celular/análisis , Proteínas de Unión al GTP/análisis , Fosfoproteínas Fosfatasas/análisis , Sinapsis/ultraestructura , Animales , Encéfalo/fisiología , Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Proteínas de Ciclo Celular/metabolismo , Fraccionamiento Celular , Immunoblotting , Inmunohistoquímica , Ratones , Fosfoproteínas Fosfatasas/metabolismo , Fosforilación , Transducción de Señal , Sinapsis/fisiología , Vesículas Sinápticas/ultraestructura , Sinaptosomas/ultraestructura , ras-GRF1