Diazoxide increases glutamate uptake by astrocytes via activating mitochondrial ATP-sensitive potassium channels / 中国临床药理学与治疗学
Article
em Zh
| WPRIM
| ID: wpr-558596
Biblioteca responsável:
WPRO
ABSTRACT
AIM: To demonstrate the effect of mitochondrial ATP-sensitive potassium channel opener,Diazoxide,on glutamate uptake activity of astrocyte. METHODS: Primary astrocytic cultures were prepared from cerebral cortices of rats within 3 days old.Glutamate uptake activity of astrocytes was measured using isotopic techniques to detect intracellular -labeled D,L-glutamate concentration.RESULTS: Diazoxide increased -glutamate uptake in a concentration-dependent manner,and could significantly reverse MPP~+-induced glutamate uptake inhibition.The above effects of Diazoxide were blocked by 5-hydroxydecanoate,a selective mitochondrial ATP-sensitive potassium channel blocker.CONCLUSION: Diazoxide increase the activity of glutamate transporters in astrocytes by opening mitochondrial ATP-sensitive potassium channel.
Texto completo:
1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Clinical Pharmacology and Therapeutics
Ano de publicação:
1999
Tipo de documento:
Article