Water-induced hypo-osmotic test for the evaluation of canine sperm membrane integrity
Anim. Reprod.
; 7(2): 70-74, 2010. tab
Article
em En
| VETINDEX
| ID: vti-9346
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
The purpose of this study was to investigate the efficiency of the water test to evaluate the functional membrane integrity of ejaculated canine sperm after collecting, cooling, and freezing-thawing. Twenty-five ejaculates were obtained from 12 stud dogs by digital manipulation. Sperm-rich fractions were evaluated, diluted, cooled at 5ºC and frozen-thawed. A conventional hypo-osmotic swelling test (HOST) using a fructose solution (60 mOsm/l) was compared with a HOST using distilled water (0 mOsm/l), for evaluation of the functional integrity of the plasma membrane in fresh, cooled, and frozen-thawed semen. Distilled water detected a higher percentage of reacted sperm in fresh semen (91.2 ± 0.1%) than the conventional HOST (90.6 ± 0.1%; P < 0.05). For cooled and frozen-thawed semen, the water test was as efficient as the conventional HOST (81.2 ± 0.3% and 80.3 ± 0.3%, respectively), for detecting functional membrane integrity (P > 0.05). Regardless of the test used, there was a decrease in the mean percentage of reacted sperm after equilibrium and freezing-thawing (P < 0.05). In conclusion, this study demonstrated that water can efficiently replace conventional fructose-based hypo-osmotic media for evaluation of the functional integrity of the plasma membrane of ejaculated fresh, cooled, and frozenthawed canine sperm.(AU)
Palavras-chave
Texto completo:
1
Base de dados:
VETINDEX
Assunto principal:
Análise do Sêmen
/
Congelamento
Limite:
Animals
Idioma:
En
Revista:
Anim. Reprod.
/
Anim. Reprod. (Online)
Ano de publicação:
2010
Tipo de documento:
Article