Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor beta1 (TGF-beta1) in rotator cuff tears
Acta cir. bras.
; 32(12): 1045-1055, dez. 2017. ilus, graf
Article
em En
| VETINDEX
| ID: vti-728520
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
Purpose:
To evaluate the effect of transforming growth factor 1 (TGF-1) on tendon-to-bone reconstruction of rotator cuff tears.Methods:
Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain. Collagen type III was observed by picric acid sirius red staining under polarized light. The level of insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was measured by the enzyme-linked immunosorbent assay (ELISA) method.Results:
Collagen type III of the H-dose group had a significant difference in histology structure compared with the L-dose group (P 0.05). The maximum load and stiffness decreased significantly in the control group compared with the values of the L-dose and H-dose groups. The stiffness among the three groups differed significantly at the same postoperative time (P 0.05). Interestingly, progressive reestablishment of collagen type III affected tendon-to-bone healing significantly in the later stages.Conclusion:
The H-dose was associated with an increased collagen type III morphology stimulated by TGF-1.(AU)Palavras-chave
Texto completo:
1
Base de dados:
VETINDEX
Assunto principal:
Colágeno Tipo III
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Fator de Crescimento Transformador beta1
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Lesões do Manguito Rotador
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras.
Ano de publicação:
2017
Tipo de documento:
Article
/
Project document