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Structure of xanthan gum and cell ultrastructure at different times of alkali stress
Luvielmo, Márcia de Mello; Borges, Caroline Dellinghausen; Toyama, Daniela de Oliveira; Vendruscolo, Claire Tondo; Scamparini, Adilma Regina Pippa.
Afiliação
  • Luvielmo, Márcia de Mello; Federal University of Rio Grande. School of Chemistry and Food. Rio Grande. Brazil
  • Borges, Caroline Dellinghausen; Universidade Federal de Pelotas. Center of Chemical, Pharmaceutical and Food Sciences. Pelotas. Brazil
  • Toyama, Daniela de Oliveira; Universidade Estadual de Campinas. Faculdade de Engenharia de Alimentos. Department of Food Sciences. Campinas. Brazil
  • Vendruscolo, Claire Tondo; Universidade Federal de Pelotas. Center of Technological Development, Biopolymeres. Pelotas. Brazil
  • Scamparini, Adilma Regina Pippa; Universidade Estadual de Campinas. Faculdade de Engenharia de Alimentos. Department of Food Sciences. Campinas. Brazil
Braz. J. Microbiol. ; 47(1): 102-109, 2016. ilus, tab, graf
Article em En | VETINDEX | ID: vti-688325
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
The effect of alkali stress on the yield, viscosity, gum structure, and cell ultrastructure of xanthan gum was evaluated at the end of fermentation process of xanthan production by Xanthomonas campestris pv. manihotis 280-95. Although greater xanthan production was observed after a 24 h-alkali stress process, a lower viscosity was observed when compared to the alkali stress-free gum, regardless of the alkali stress time. However, this outcome is not conclusive as further studies on gum purification are required to remove excess sodium, verify the efficiency loss and the consequent increase in the polymer viscosity. Alkali stress altered the structure of xanthan gum from a polygon-like shape to a star-like form. At the end of the fermentation, early structural changes in the bacterium were observed. After alkali stress, marked structural differences were observed in the cells. A more vacuolated cytoplasm and discontinuities in the membrane cells evidenced the cell lysis. Xanthan was observed in the form of concentric circles instead of agglomerates as observed prior to the alkali stress. (AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Adesivos / Xanthomonas campestris / Concentração de Íons de Hidrogênio Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Adesivos / Xanthomonas campestris / Concentração de Íons de Hidrogênio Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2016 Tipo de documento: Article